BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0580.0016.p (293 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yjbV [H] COG0351 Hydroxymethylpyrimidine/phosphomethylpyrimid... 293 2e-79 lin0342 [H] COG0351 Hydroxymethylpyrimidine/phosphomethylpyrimid... 241 7e-64 SA1896 [H] COG0351 Hydroxymethylpyrimidine/phosphomethylpyrimidi... 232 6e-61 SP0726 [H] COG0351 Hydroxymethylpyrimidine/phosphomethylpyrimidi... 224 1e-58 BS_thiD [H] COG0351 Hydroxymethylpyrimidine/phosphomethylpyrimid... 216 3e-56 >BS_yjbV [H] COG0351 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase Length = 271 Score = 293 bits (751), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 149/262 (56%), Positives = 201/262 (76%) Query: 25 NVFKALTVAGSDSGGGAGIQADLKTFQELGVYGMSALTAVTAQNTLGVQSVYPLEGEAVA 84 +++KALT+AGSDSGGGAGIQAD+KTFQEL V+GMSA+TAVTAQNTLGV V+PL E + Sbjct: 2 SIYKALTIAGSDSGGGAGIQADIKTFQELDVFGMSAITAVTAQNTLGVHGVHPLTVETLR 61 Query: 85 AQLDSIGADLTPDAVKTGMLFSSGLIHTVAQKFRQYGWTKLVIDPVMVAKGGSPLLQQEA 144 Q+D++ DL PDAVKTGML+++ +I VA+K +YG+ ++++DPVM+AKGG+ LL+ E+ Sbjct: 62 QQIDAVAEDLRPDAVKTGMLWNADMIEEVARKIDEYGFNRVIVDPVMIAKGGASLLRDES 121 Query: 145 VQALIADLLPLSLAITPNIPEAEILTGMKISDMADREAAARLVHAMGPRHVILKGGHDEE 204 V L L+P S AITPN+PEAE LTGM IS + DR+ AA + MG +HVI+KGGH E Sbjct: 122 VATLKELLIPRSYAITPNVPEAETLTGMTISSLDDRKKAAEQLVKMGAQHVIIKGGHQPE 181 Query: 205 GALVTDLLYDGEKFIYLQSPRIATRHTHGTGCTFSAALTAGLAQGQRVPEAVHTAKAFIQ 264 +TDLL+DG F+ + P I T+HTHGTGCTF+AALTA A+G + +A A F++ Sbjct: 182 DNHITDLLFDGSMFMQITHPYINTKHTHGTGCTFAAALTAQTAKGDSIHQAFEVAANFVR 241 Query: 265 AAIEDGINIGAGNGPTNHFAYQ 286 A+E+ + IG+G+GPTNHFA++ Sbjct: 242 EAVENTLGIGSGHGPTNHFAFK 263 >lin0342 [H] COG0351 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase Length = 182 Score = 241 bits (616), Expect = 7e-64, Method: Compositional matrix adjust. Identities = 0/259 (0%), Positives = 0/259 (0%), Gaps = 1/259 (0%) Query: 28 KALTVAGSDSGGGAGIQADLKTFQELGVYGMSALTAVTAQNTLGVQSVYPLEGEAVAAQL 87 + + G + T S+ L + + Sbjct: 5 HVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKGQLLVTRRALSKKAWPGVWTN 64 Query: 88 DSIGADLTPDAVKTGMLFSSGLIHTVAQKFRQYGWTKLVIDPVMVAKGGSPLLQQEAVQA 147 G ++ + ++ V + + + + Sbjct: 65 SVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDFRYRATDPSGIVENEVCPVFAAR 124 Query: 148 LIADLLPLSLAITPNIPEAEILTGMKISDMADREAAARLVHAMGPRHVILKGGHDEEGAL 207 Sbjct: 125 - 183 Query: 208 VTDLLYDGEKFIYLQSPRIATRHTHGTGCTFSAALTAGLAQGQRVPEAVHTAKAFIQAAI 267 Sbjct: 184 243 Query: 268 EDGINIGAGNGPTNHFAYQ 286 Sbjct: 244 262 >SA1896 [H] COG0351 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase Length = 276 Score = 232 bits (591), Expect = 6e-61, Method: Compositional matrix adjust. Identities = 118/258 (45%), Positives = 163/258 (63%), Gaps = 1/258 (0%) Query: 29 ALTVAGSDSGGGAGIQADLKTFQELGVYGMSALTAVTAQNTLGVQSVYPLEGEAVAAQLD 88 ALT+AG+D GGAG+ ADLK+F GVYGM +T++ AQNTLGVQ ++ L + V QLD Sbjct: 7 ALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVAQNTLGVQHIHNLNHQWVDEQLD 66 Query: 89 SIGADLTPDAVKTGMLFSSGLIHTVAQKFRQYGWTKLVIDPVMVAKGGSPLLQQEAVQAL 148 S+ D P A+KTGM+ ++ + T+ Q+ VIDPVM+AK G L+ + Q L Sbjct: 67 SVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIPYVIDPVMLAKSGDSLMDNDTKQNL 126 Query: 149 IADLLPLSLAITPNIPEAEILTGMKISDMADREAAARL-VHAMGPRHVILKGGHDEEGAL 207 LLPL+ +TPN+PEAE +TG+ I A R+ ++ +G + +I+KGGH + + Sbjct: 127 QHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQAGRIFINEIGSKGIIIKGGHSNDTDI 186 Query: 208 VTDLLYDGEKFIYLQSPRIATRHTHGTGCTFSAALTAGLAQGQRVPEAVHTAKAFIQAAI 267 D L+ E ++ R T+HTHGTGCTFSA +TA LA+G+ + EAVH AK FI +I Sbjct: 187 AKDYLFTNEGVQTFENERFKTKHTHGTGCTFSAVITAELAKGRPLFEAVHKAKKFISMSI 246 Query: 268 EDGINIGAGNGPTNHFAY 285 + IG G GP NHFAY Sbjct: 247 QYTPEIGRGRGPVNHFAY 264 >SP0726 [H] COG0351 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase Length = 263 Score = 224 bits (571), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 125/271 (46%), Positives = 176/271 (64%), Gaps = 11/271 (4%) Query: 18 MTNHPITNVFKALTVAGSDSGGGAGIQADLKTFQELGVYGMSALTAVTAQNTLGVQSVYP 77 MT P+ ALT+AG+D GGAGI ADLK+FQ VYGM+ +T++ AQNT GVQ + Sbjct: 1 MTYLPV-----ALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVAQNTRGVQLIEH 55 Query: 78 LEGEAVAAQLDSIGADLTPDAVKTGMLFSSGLIHTVAQKFRQYGWTKLVIDPVMVAKGGS 137 + + + AQL+S+ +D+ P AVKTGML ++ ++ + Q + + V+DPVMVA G Sbjct: 56 VSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEII-QPYLKKLDCPYVLDPVMVATSGD 114 Query: 138 PLLQQEAVQALIADLLPLSLAITPNIPEAEILTGMKISDMADREAAARLV-HAMGPRHVI 196 L+ A L +LLPL+ ITPN+PEAE + G I D D + A RL+ GP+ V+ Sbjct: 115 ALIDSNARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRAGRLILKEFGPQSVV 174 Query: 197 LKGGHDEEGALVTDLLY-DGEKFIYLQSPRIATRHTHGTGCTFSAALTAGLAQGQRVPEA 255 +KGGH + GA D L+ E+F++ +SPRI T HTHGTGCTF+A +TA LA+G+ + +A Sbjct: 175 IKGGHLKGGA--KDFLFTKNEQFVW-ESPRIQTCHTHGTGCTFAAVITAELAKGKSLYQA 231 Query: 256 VHTAKAFIQAAIEDGINIGAGNGPTNHFAYQ 286 V AKAFI AI+D +G G+GP NH ++ Sbjct: 232 VDKAKAFITKAIQDAPQLGHGSGPVNHTTFK 262 >BS_thiD [H] COG0351 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase Length = 266 Score = 216 bits (551), Expect = 3e-56, Method: Compositional matrix adjust. Identities = 0/271 (0%), Positives = 0/271 (0%), Gaps = 5/271 (1%) Query: 25 NVFKALTVAGSDSGGGAGIQADLKTFQELGVYGMSALTAVTAQ--NTLGVQSVYPLEGEA 82 ALT+AG+D GGAGIQADLKTF LG YG S +TA+ AQ + + A Sbjct: 2 KRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVYRIEPDFVA 61 Query: 83 VAAQLDSIGADLTPDAVKTGMLFSSGLIHTVAQKFRQYGWTKLVIDPVMVAKGGSPLLQQ 142 + + + Q L + + Sbjct: 62 AQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNVVLDTVMLAKSGDPLLSPSA 121 Query: 143 EAVQALIADLLPLSLAITPNIPEAEILTGM-KISDMADREAAARLVHAMGPRHVILKGGH 201 A + A + + + + L G + Sbjct: 122 VATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLEQGRSLLAMGCGAVLMKGGHLD 181 Query: 202 DEEGALVTDLLYDGEKFIYLQSPRIATRHTHGTGCTFSAALTAGLAQGQRVPEAVHTAKA 261 DE+ ++F + T T T AAL + + + Sbjct: 182 DEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAALAALRPRHTNWADTVQEAKSWLS 241 Query: 262 FIQAAIEDGINIGAGNGPTNHFAYQTRLRGQ 292 A + + A+ Sbjct: 242 SALAQADTLEVGHGIGPVHHFHAW-- 270 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.133 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,493,739 Number of extensions: 892735 Number of successful extensions: 1891 Number of sequences better than 1.0e-05: 32 Number of HSP's gapped: 1849 Number of HSP's successfully gapped: 32 Length of query: 293 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 187 Effective length of database: 26,153,027 Effective search space: 4890616049 Effective search space used: 4890616049 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)