BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0579.0061.p (416 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0293 [GEPR] COG0477 Permeases of the major facilitator superfa... 62 1e-09 SPy0543 [GEPR] COG0477 Permeases of the major facilitator superf... 59 1e-08 all2673 [GEPR] COG0477 Permeases of the major facilitator superf... 58 2e-08 TM0342 [GEPR] COG0477 Permeases of the major facilitator superfa... 57 4e-08 PAB0724 [GEPR] COG0477 Permeases of the major facilitator superf... 57 8e-08 >BH0293 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 418 Score = 62.4 bits (150), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 76/378 (20%), Positives = 158/378 (41%), Gaps = 14/378 (3%) Query: 3 RQEKSTTAPSLLRNRFLQTILLSSVLLQIGIWVRNFAILLYVADRTNNDPYAISLISVAE 62 Q +S SLLRN+ + ++S+ + + YV D + + ++ +A Sbjct: 10 EQPQSQGLSSLLRNQKFLLLWVASIFSGLSFSIYFLTKSWYVVDELGLKTF-LGVVLMAM 68 Query: 63 FAPIFVFSFIGGTFADRWRPKRTMIWCDXXXXXXXXXXXXXXHYGSWHSVYLV---AFIS 119 P + IGG ADR+R + T+++C + +++ + AF Sbjct: 69 TVPRVLLMMIGGVLADRFR-RSTIMFCSLITRSVLLFGMFILQFLGVINIWTLIGFAFCI 127 Query: 120 AILSQFSQPSSMRLFKYHVAEEQLQQGMALFQSLMAIFMVLGPMLGTFVYSTFGLETSIA 179 IL F P++ + V ++ L + + QS + +++GPM+ ++ + E Sbjct: 128 GILDAFFWPANYAILPSIVKKDHLTRANSFLQSTNHLTLIIGPMIAGWLITITAYEWIFV 187 Query: 180 IMGVVFLLSALVLIRLPEDNLKPKTDAAKGQFRKEFIEGFRYVWNSKV-LRMLGLAFIXX 238 + + ++ A+++ R+ E KP+T K+ EG+ Y+ +S V L ++ ++ Sbjct: 188 TIMCLLIIGAILIFRVKEPFDKPETKRLPSPL-KDLTEGYSYLRHSPVLLSLMWMSVFVN 246 Query: 239 XXXXXXXXXXXXFIVTERLGKSKEFLQYLLMVNGAAMLIGGGVVAVFAKRVPPQLLLAIG 298 IV ERL L YL +++G ++ + R LL+ I Sbjct: 247 FFITGPGTMAIPLIVEERLKGDPLELSYLESTIAGGLILGAIMIGIINLRSKRGLLIIIR 306 Query: 299 MLAGAVCTIIVGYSTSVPVTLTVQFLNGLVFPCIHIGISTMILKWSHSSIVGRVNGVLN- 357 + V ++G S+ + V + G+ I ++ + + +GRV G+++ Sbjct: 307 LTVMGVAIALLGQVVSLWQAIFVLAILGMGISIGDIPARAVVQEKTDFDKIGRVMGMMST 366 Query: 358 ------PMFVGMMVISMS 369 P+ GM +++S Sbjct: 367 ASSGLIPLSYGMTSVALS 384 >SPy0543 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 402 Score = 58.9 bits (141), Expect = 1e-08, Method: Compositional matrix adjust. Identities = 53/219 (24%), Positives = 103/219 (47%), Gaps = 11/219 (5%) Query: 15 RNRFLQTILLSSVLLQIGIWVRNFAILLYVADRTNNDPYAISLISVAEFAPIFV---FSF 71 R + +Q ++ S V+ +IG + +FA ++A +P ++SL++V + + F+ Sbjct: 4 RKKNIQYLVYSKVIYRIGDVMFDFANNTFLAGL---NPASLSLVAVYQSLESVIGVLFNL 60 Query: 72 IGGTFADRWRPKRTMIWCDXXXXXXXXXXXXXXHYGSW--HSVYLVAFISAILSQFSQPS 129 GG AD ++ K+ +I + W +++ L I A +S FS PS Sbjct: 61 FGGVIADSFKRKKIIITTNILCGTACLVLSFLTK-EQWLVYAIVLTNVILAFMSAFSSPS 119 Query: 130 SMRLFKYHVAEEQLQQGMALFQSLMAIFMVLGPMLGTFVYSTFGLETSIAIMGVVFLLSA 189 K V ++ + Q +L ++ + V PM+ F+Y G+ + + G+ FL++A Sbjct: 120 YKAFTKEIVKKDSISQLNSLLETTSTVIKVTVPMVAIFLYKLLGIHGVLLLDGLSFLIAA 179 Query: 190 LVL-IRLP-EDNLKPKTDAAKGQFRKEFIEGFRYVWNSK 226 L++ LP D + K + + GF+YV++ K Sbjct: 180 LLISFILPVNDEVVIKEKVTIREIFNDLKIGFKYVYSHK 218 >all2673 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 437 Score = 58.2 bits (139), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 72/368 (19%), Positives = 142/368 (38%), Gaps = 13/368 (3%) Query: 17 RFLQTILLSSVLLQIGIWVRNFAILLYVADRTNNDPYAISLISVAEFAPIFVFSFIGGTF 76 R I L ++ IG ++ FA+ ++V D+T + A++L+ P + + G Sbjct: 2 RTFLIIWLGQIISTIGSFMTVFALTIWVWDKTGSAT-ALALVGFFAQMPKILITTFAGIA 60 Query: 77 ADRWRPKRTMIWCDXXXXXXXXXXXXX---XHYGSWHSVYLVAFISAILSQFSQPSSMRL 133 DR+ + MI + WH +Y++ + Q + Sbjct: 61 VDRFNRRFLMILAEVVAVLCTLAIAILYLNQQLQVWH-LYIIVMLYGGFGQLQVLAYSAS 119 Query: 134 FKYHVAEEQLQQGMALFQSLMAIFMVLGPMLGTFVYSTFGLETSIAIMGVVFLLS--ALV 191 V +EQ + +L ++ + P L F+Y GL+ I F + L+ Sbjct: 120 ISLLVPKEQFTRAESLGSAVNYSAAIFSPALAGFIYPRLGLQAIFWIDLTTFAAAFITLL 179 Query: 192 LIRLPEDNLKPKTDAAKGQFRKEFIEGFRYVWNSKVLRMLGLAFIXXXXXXXXXXXXXXF 251 ++++P+ ++P ++ GFRY+W + L + +AF Sbjct: 180 IVKIPQPAVEPNHRDNTESLWQKLTFGFRYIWANPSLTAMAIAFTLFAIPNDISKVLYTP 239 Query: 252 IVTERLGKSKEFLQYLLMVNGAAMLIGGGVVAV---FAKRVPPQLLLAIGMLAGAVCTII 308 ++ R G + E L + G +IG +++ F +R+ LL G + + + Sbjct: 240 MILARTGGNSEILGTVTTAAGIGGVIGAIALSIWGGFHRRIHGMLL---GFIGHGIFRTL 296 Query: 309 VGYSTSVPVTLTVQFLNGLVFPCIHIGISTMILKWSHSSIVGRVNGVLNPMFVGMMVISM 368 +G +V V + FL+ + P + + + + GRV + V + I+ Sbjct: 297 IGLGQTVSVWMGAHFLSSTLIPLFYSSSNAIWYATIPPGLQGRVLAADQLIGVTVAAITP 356 Query: 369 SFAGALKD 376 AG L D Sbjct: 357 LIAGFLAD 364 >TM0342 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 413 Score = 57.4 bits (137), Expect = 4e-08, Method: Compositional matrix adjust. Identities = 56/217 (25%), Positives = 96/217 (44%), Gaps = 5/217 (2%) Query: 15 RNRFLQTILLSSVLLQIGIWVRNFAILLYVADRTNNDPYAISLISVAEFAPIFVFSFIGG 74 RN FL + L + +G V+ AI L+V D T++ A++L++VA P +F+ I G Sbjct: 9 RNFFL--LALGRFVSILGSSVQAVAIPLFVLDLTHSGS-AVALMTVAYMVPRILFTPIAG 65 Query: 75 TFADRWRPKRTMIWCDXXXXXXXXXXXXXXHYGS--WHSVYLVAFISAILSQFSQPSSMR 132 DR K M+ D S +Y+ AI+ + Sbjct: 66 IVGDRGDRKFLMVSMDFLRGFLICFLALLAFRNSITLPVLYVSQIAMAIMDNLFDIPTGA 125 Query: 133 LFKYHVAEEQLQQGMALFQSLMAIFMVLGPMLGTFVYSTFGLETSIAIMGVVFLLSALVL 192 +F V ++ L + ++ + +LGP LG +Y +G+ + GV F+LSA+ Sbjct: 126 MFPDIVPQDFLMRANSIMGMVRIFPQILGPALGGVIYGFYGIAIVFLVNGVSFVLSAISE 185 Query: 193 IRLPEDNLKPKTDAAKGQFRKEFIEGFRYVWNSKVLR 229 I + K A Q ++ EGF ++WN +++R Sbjct: 186 IFIVYSRKVEKKPAKFSQILEDLKEGFLFIWNHELIR 222 >PAB0724 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 418 Score = 56.6 bits (135), Expect = 8e-08, Method: Compositional matrix adjust. Identities = 49/205 (23%), Positives = 98/205 (47%), Gaps = 4/205 (1%) Query: 30 QIGIWVRNFAILLYVADRTNNDPYAISLISVAEFAPIFVFSFIGGTFADRWRPKRTMIWC 89 Q+G ++ A+ LYV D T++ ++L +A+ P + + I G F DR+ K+ M+ Sbjct: 16 QLGWAIQEVALPLYVLDVTHSAKI-MTLFVLADLIPSIITTPIAGVFGDRYDRKKIMVGF 74 Query: 90 DXXXXXXXXXXXXXXHYGSWHSVYLVAFISAILSQFSQPSSMRLFKYHVAEEQLQQGMAL 149 D ++ + + +V I AI++ F + ++ V E+L++ ++ Sbjct: 75 DLIRGVLLFLVIAF-NFLTIRCLLVVQVIMAIMNAFFSAGTGAMYPDLVKPEELERANSI 133 Query: 150 FQSLMAIFMVLGPMLGTFVYSTFGLETSIAIMGVVFLLSALVLIRLPEDNLKPKTDAAKG 209 S I ++GP +G FVY G++ +I + + F S L I + + K + Sbjct: 134 VMSASIISRLVGPAIGGFVYGFGGIKLAILMNAISFFGSGLFEIFIKYE-WKTRNIEGFS 192 Query: 210 QFRKEFIEGFRYVW-NSKVLRMLGL 233 F ++ EG +++ NS + ++GL Sbjct: 193 DFVRDIKEGLKFIMSNSFISTLIGL 217 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.140 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,882,510 Number of extensions: 936039 Number of successful extensions: 2701 Number of sequences better than 1.0e-05: 9 Number of HSP's gapped: 2710 Number of HSP's successfully gapped: 9 Length of query: 416 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 307 Effective length of database: 25,807,025 Effective search space: 7922756675 Effective search space used: 7922756675 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 117 (49.7 bits)