BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0579.0034.p (159 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC0451 [T] COG0642 Signal transduction histidine kinase 117 7e-27 BS_resE_4 [T] COG0642 Signal transduction histidine kinase 81 8e-16 lin1543 [T] COG0642 Signal transduction histidine kinase 80 2e-15 lin2727 [T] COG0642 Signal transduction histidine kinase 78 5e-15 BS_phoR_3 [T] COG0642 Signal transduction histidine kinase 77 1e-14 >CAC0451 [T] COG0642 Signal transduction histidine kinase Length = 416 Score = 117 bits (293), Expect = 7e-27, Method: Compositional matrix adjust. Identities = 67/160 (41%), Positives = 95/160 (59%), Gaps = 4/160 (2%) Query: 1 MDAKTIQFKKDRIPVKNLIQKALEPVMIPM-DIKGQTVSIAGDDDVSFAGDFNWTVEAVI 59 +DA I FK ++ ++ + +++ ++ PM +K Q VS+ D F D W +EA Sbjct: 256 LDAGAIDFKNEKRSIEETVCESI-SLLKPMYTLKNQKVSVESTDKAYFKHDKKWLLEAFS 314 Query: 60 NILKNGVEHTHEGGAITITFSENTLFTEIVIADNGKGIPKEDLPYIFKRFYKGKNA-GEG 118 NI+KN +EHT + G I I + + ++ I DNG GI K+DLP+IF+RFYKGK + Sbjct: 315 NIIKNCIEHTGDNGEIKIHVYRSPIMVKVTIEDNGSGISKDDLPHIFERFYKGKGVYNKS 374 Query: 119 SIGIGLAMAHSIIASQNGVIDVTSGSGKGTQFRIKF-YKQ 157 S GIGL+MA SII +Q G I V S GT F I F Y+Q Sbjct: 375 STGIGLSMAKSIIENQGGFITVKSAVSAGTIFNIVFLYEQ 414 >BS_resE_4 [T] COG0642 Signal transduction histidine kinase Length = 269 Score = 80.9 bits (198), Expect = 8e-16, Method: Compositional matrix adjust. Identities = 49/126 (38%), Positives = 70/126 (55%), Gaps = 10/126 (7%) Query: 38 SIAGDDDVS-----FAGDFNWTVEAVINILKNGVEHTHEGGAITITFSENTLFTEIVIAD 92 +IA D D+S F D + + N++ N + HT GG+++I+ +I I D Sbjct: 141 NIALDHDISLTEEEFMFDEDKMEQVFTNLIDNALRHTSAGGSVSISVHSVKDGLKIDIKD 200 Query: 93 NGKGIPKEDLPYIFKRFYKGKNA---GEGSIGIGLAMAHSIIASQNGVIDVTSGSGKGTQ 149 +G GIP+EDLP+IF+RFYK A G G+GLA+ +I+ + NG I V S KGT Sbjct: 201 SGSGIPEEDLPFIFERFYKADKARTRGRAGTGLGLAIVKNIVEAHNGSITVHSRIDKGTT 260 Query: 150 FRIKFY 155 F FY Sbjct: 261 F--SFY 264 >lin1543 [T] COG0642 Signal transduction histidine kinase Length = 182 Score = 79.7 bits (195), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 0/153 (0%), Positives = 0/153 (0%), Gaps = 5/153 (3%) Query: 6 IQFKKDRIPVKNLIQKALEPVMIPMDIKGQTVSIAGDDDVSFAGDFNWTVEAVINILKNG 65 Sbjct: 320 379 Query: 66 VEHTHEGGAITITFSENTLFTEIVIADNGKGIPKEDLPYIFKRFYKG----KNAGEGSIG 121 Sbjct: 380 - 438 Query: 122 IGLAMAHSIIASQNGVIDVTSGSGKGTQFRIKF 154 Sbjct: 439 471 >lin2727 [T] COG0642 Signal transduction histidine kinase Length = 182 Score = 78.2 bits (191), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 0/116 (0%), Positives = 0/116 (0%), Gaps = 3/116 (2%) Query: 44 DVSFAGDFNWTVEAVINILKNGVEHTHEGGAITITFSENTLFTEIVIADNGKGIPKEDLP 103 Sbjct: 343 402 Query: 104 YIFKRFYKG---KNAGEGSIGIGLAMAHSIIASQNGVIDVTSGSGKGTQFRIKFYK 156 Sbjct: 403 458 >BS_phoR_3 [T] COG0642 Signal transduction histidine kinase Length = 431 Score = 77.0 bits (188), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 8/130 (6%), Positives = 23/130 (17%), Gaps = 4/130 (3%) Query: 31 DIKGQTVSIAGDDDVSF-AGDFNWTVEAVINILKNGVEHTHEGGAITITFSENTLFTEIV 89 + + N + ++ + +TH+ + E Sbjct: 144 LNLLRYPEFTQYLKTRDFSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGA 203 Query: 90 IADNGKGIPKEDLPYIFKRFY---KGKNAGEGSIGIGLAMAHSIIASQNGVIDVTSGSGK 146 + + E + A+ +Q V Sbjct: 204 RRNFFANVSHELRTPLTVLQGYLEMMNEQPLEGAVREKALHTMREQTQRMEGLVKQLLTL 263 Query: 147 GTQFRIKFYK 156 + Sbjct: 264 SKIEAAPTHL 273 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.138 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 12,035,916 Number of extensions: 548498 Number of successful extensions: 1499 Number of sequences better than 1.0e-05: 157 Number of HSP's gapped: 1459 Number of HSP's successfully gapped: 157 Length of query: 159 Length of database: 38,378,431 Length adjustment: 98 Effective length of query: 61 Effective length of database: 27,075,699 Effective search space: 1651617639 Effective search space used: 1651617639 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 111 (47.4 bits)