BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0579.0024.p (433 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Cj0484 [GEPR] COG0477 Permeases of the major facilitator superfa... 113 8e-25 SPAC1002.16c [GEPR] COG0477 Permeases of the major facilitator s... 91 4e-18 dgoT [GEPR] COG0477 Permeases of the major facilitator superfamily 90 6e-18 SPAC11D3.18c [GEPR] COG0477 Permeases of the major facilitator s... 87 6e-17 BS_yhaU [P] COG0475 Kef-type K+ transport systems, membrane comp... 87 7e-17 >Cj0484 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 410 Score = 113 bits (282), Expect = 8e-25, Method: Compositional matrix adjust. Identities = 105/403 (26%), Positives = 186/403 (46%), Gaps = 19/403 (4%) Query: 3 NSNKGGFVASR--WLRLMPIVFVTYSLAYLDRANYSFGAAAGMAKDLHISAGMSSXXXXX 60 NS K V + W R++P++ + + L+ LDR N SF + + D I + Sbjct: 5 NSIKLELVCKKISW-RILPLIVLMFCLSMLDRTNISF-VKSHIEIDAGIGEAAYALGAGI 62 Query: 61 XXXXXXXXQVPGAHYAENKSAKKLVFGSLILWGLLATATGIVHNVHFLIIIRFCLGVVES 120 +VP + AK + +I WGL+ A + ++RF LG+ E+ Sbjct: 63 FFIGYAIFEVPSNLFLHKLGAKIWLSRIMITWGLVTMAMIFIQGEISFYVLRFLLGLTEA 122 Query: 121 AVMPAMLVFLSHWFTKKERSRANTFLILGNPVTVLWMSIVSGYLVSAV------GWRWMF 174 P ++++LS++F RS+A +G P+ ++ S++SG+++ W+WMF Sbjct: 123 GFSPGIILYLSYFFPAIYRSKAYGIYQMGVPIAFVFGSLISGFILDYTPNIYFKNWQWMF 182 Query: 175 IIEGLPPIIWAFFWWKLVNDKPSETNWLTSQEKKELESALHEEQKGLKPVKNYG--EAFR 232 +IEG ++ F ++ P + WL +EK L H E K K+Y + F+ Sbjct: 183 LIEGGITVLVGIFCLFYLDSHPKDAKWLDIKEKDILLK--HIEISNTKA-KDYSIKDIFK 239 Query: 233 SPLVIRLCLQYFFWSIGVYGFVMWLPSIIKAAPDMSM-VATGWLTSVPYVLAVIGMLTAS 291 S LV + YF + VYG + +LPS I +++ G L ++P++ I + + Sbjct: 240 SILVWKFVFVYFCIQLSVYGVLFYLPSKIAQILQINVGFEVGLLNAIPWIFVFIALPIFT 299 Query: 292 YFSDKTQNRKAFVWPFLMVGAVAFYASYLLGADHFWISFILLIIAGGAMYAPYGPFFAIM 351 +DK + FL++ +++ AS + ++ FI L G + P F+ + Sbjct: 300 SLADKKHSWNLHAILFLLLASLSMIASTFVTNLAAFLFFISLAAIGFIVIQPI--FWNLP 357 Query: 352 PEILPRNVSGGAMALINSMGALGSFAGSYIVGYL-NSFTGGFG 393 ++L + A+ALI S+G LG F + Y+ N F FG Sbjct: 358 TQVLKGKGAAAAIALIGSLGNLGGFVAPTLKTYIENHFGVEFG 400 >SPAC1002.16c [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 499 Score = 90.5 bits (223), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 79/320 (24%), Positives = 140/320 (43%), Gaps = 31/320 (9%) Query: 1 MNNSNKGGFVASRWLRLMPIVFVTYSLAYLDRANYSFGAAAGMAKDLHISAGMSSXXXXX 60 + S + + L L P + + Y +A+LDR+N AG+ +DL + + Sbjct: 43 LTKSERDSLLFRLDLVLAPTIMILYLVAFLDRSNIGNAKVAGLPEDLKLKGDQFNIIASV 102 Query: 61 XXXXXXXXQVPGAHYAENKSAKKLVFGSLILWGLLATATGIVHNVHFLIIIRFCLGVVES 120 ++P + K+++ +I + L TG HN L+ R LG E+ Sbjct: 103 FYVTFILFEMPTTLLMKKVQPKRMLAFIVISYSLTTIFTGFCHNFGGLLAARLVLGFCEA 162 Query: 121 AVMPAMLVFLSHWFTKKERSRANTFLILGNPVT----------VLWMSIVSGYLVSAVGW 170 + P + ++L+ +++ E + +L + ++ VL M V G+ GW Sbjct: 163 GLFPCLALYLTMIYSRVELAPRIAYLFASSALSGAFGGLFAYAVLHMDGVGGF----AGW 218 Query: 171 RWMFIIEGLPPIIWAFFWWKLVNDKPSETNWL--TSQE---KKELE-----SALHEEQKG 220 RW+FIIEGL + + ++ + ++ +L T QE K++LE A H + KG Sbjct: 219 RWLFIIEGLIGFVCGVAVYFIIPNDITKAWFLSKTHQEMMRKRQLERAADLEAAHFDWKG 278 Query: 221 LKPVKNYGEAFRSPLVIRLCLQYFFWSIGVYGFVMWLPSIIKAAPDMSMVATGWLTSVPY 280 +K AF V L F +YGF +LP+II S+ + ++T Y Sbjct: 279 VK------SAFTDFKVYLYALSEFGQDTCLYGFSTFLPAIISGMGYTSL-SVQYMTIPVY 331 Query: 281 VLAVIGMLTASYFSDKTQNR 300 +L + AS+ SD+ +R Sbjct: 332 ILGAATYIAASFLSDRFHHR 351 >dgoT [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 445 Score = 90.1 bits (222), Expect = 6e-18, Method: Compositional matrix adjust. Identities = 92/423 (21%), Positives = 189/423 (44%), Gaps = 28/423 (6%) Query: 3 NSNKGGFVASRWLRLMPIVFVTYSLAYLDRANYSFGAAAGMAKDLHISAGMSSXXXXXXX 62 N+ K G R+L L+ ++F+T + Y+DRAN + A+A + ++ I+ Sbjct: 21 NAAKPG--RRRYLTLV-MIFITVVICYVDRANLAV-ASAHIQEEFGITKAEMGYVFSAFA 76 Query: 63 XXXXXXQVPGAHYAENKSAKKLVFGSLILWGLLATATGIVHNVHFLIIIRFCLGVVESAV 122 Q+PG + + ++ F ++ W + G + LI +R G+ E+ Sbjct: 77 WLYTLCQIPGGWFLDRVGSRVTYFIAIFGWSVATLFQGFATGLMSLIGLRAITGIFEAPA 136 Query: 123 MPAMLVFLSHWFTKKERSRANTFLILGNPVTVLWMSIVSGYLVSAVGWRWMFIIEGLPPI 182 P ++ WF + ER+ A F G V + +++ + ++ + W W+FI+ G I Sbjct: 137 FPTNNRMVTSWFPEHERASAVGFYTSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGI 196 Query: 183 IWAFFWWKLVNDKPSETNWLTSQEKKEL---------ESALHEEQKGLKPVKNYGEAFRS 233 IW+ W+K V P T ++ E + ++ + +E + K++ F Sbjct: 197 IWSLIWFK-VYQPPRLTKGISKAELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHR 255 Query: 234 PLVIRLCLQYFFWSIGVYGFVMWLPSIIKAAPDMSMVATGWLTSVPYVLAVIGMLTASYF 293 L I + L F + ++ F+ W P+ + ++ + G++T+VP++ A +G+L + + Sbjct: 256 KL-IGVYLGQFAVASTLWFFLTWFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWV 314 Query: 294 SDKTQNRKAFVWPFLMVGAVA---FYASYLLGADHFWISFILLIIAGGAMYAPYGPFFA- 349 +D RK F F + ++ ++GA++ +++ + A +G FA Sbjct: 315 ADLLV-RKGFSLGFARKTPIICGLLISTCIMGANYTNDPMMIMCLMALAF---FGNGFAS 370 Query: 350 ----IMPEILPRNVSGGAMALINSMGALGSFAGSYIVGYLNSFTGGFGASYIFMAGSLLL 405 ++ + P + G + N G LG +VGYL GF + ++++ L+ Sbjct: 371 ITWSLVSSLAPMRLIGLTGGVFNFAGGLGGITVPLVVGYLAQ-GYGFAPALVYISAVALI 429 Query: 406 SAI 408 A+ Sbjct: 430 GAL 432 >SPAC11D3.18c [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 498 Score = 87.0 bits (214), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 79/314 (25%), Positives = 144/314 (45%), Gaps = 18/314 (5%) Query: 15 LRLMPIVFVTYSLAYLDRANYSFGAAAGMAKDLHISAGMSSXXXXXXXXXXXXXQVPGAH 74 L + PI+ Y +A+LDR+N A AGM + L + + + P Sbjct: 59 LVISPIIGFLYLMAFLDRSNIGNAAVAGMTEALSLYGERLNVAVSIFYVLYILVETPSVV 118 Query: 75 YAEN-KSAKKLVFGSLILWGLLATATGIVHNVHFLIIIRFCLGVVESAVMPAMLVFLSHW 133 + K+++ L F S W + +G + + LI R LG++E + PA+ ++L+ Sbjct: 119 LVKRIKASRMLAFISFA-WSMTVLFSGFMSSYGGLIATRLILGLLEGCLFPALNLYLTTH 177 Query: 134 FTKKERSRANTFL-----ILGNPVTVLWMSIVSGYLVSAVGWRWMFIIEGLPPIIWAFFW 188 +T+KE+ + ++L + G + ++ + + GW+W++I+EGL I Sbjct: 178 YTRKEQCQRLSYLFASAGLAGAFGGLFAYALEQVHAGNKEGWQWIYIVEGLVSFIGVPLC 237 Query: 189 WKLVNDKPSETNWLTSQE------KKELESALHEEQK-GLKPVKNYGEAFRSPLVIRLCL 241 + DK +LT +E ++++ + H EQ V+ +AF+ P V Sbjct: 238 LFALPDKMENAWFLTREEREVAIIRRDINARYHGEQHFEWSEVR---KAFKDPKVYVSAT 294 Query: 242 QYFFWSIGVYGFVMWLPSIIKAAPDMSMVATGWLTSVPYVLAVIGMLTASYFSDKTQNRK 301 F + +YGF +LP IIK + + T ++T Y+ VI L ++ SD+TQ R Sbjct: 295 SQFCADMVLYGFSSFLPVIIKGLGFVGL-QTNYMTIPVYIAGVISFLFVAWLSDRTQLRA 353 Query: 302 AFVWPFLMVGAVAF 315 ++ V AV + Sbjct: 354 VYLISASTVVAVGY 367 >BS_yhaU [P] COG0475 Kef-type K+ transport systems, membrane components Length = 444 Score = 86.7 bits (213), Expect = 7e-17, Method: Compositional matrix adjust. Identities = 102/447 (22%), Positives = 186/447 (41%), Gaps = 37/447 (8%) Query: 1 MNNSNKGGFVASRWLRLMPIVFVTYSLAYLDRANYSFGAAAGMAKDLHISAGMSSXXXXX 60 ++ KG V +R+L L+ I+F+ ++ Y DRA S A +AK+L +SA Sbjct: 6 VDEKKKG--VHTRYLILL-IIFIVTAVNYADRATLSI-AGTEVAKELQLSAVSMGYIFSA 61 Query: 61 XXXXXXXXQVPGAHYAENKSAKKLVFGSLILWGLLATATGIVHNVHF------LIIIRFC 114 Q+PG + +KK+ SL W L G V + +RF Sbjct: 62 FGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGFVDMFPLAWAGISMFFMRFM 121 Query: 115 LGVVESAVMPAMLVFLSHWFTKKERSRANTFLILGNPVTVLWMSIVSGYLVSAVGWRWMF 174 LG E+ PA ++ WF KER A+ ++ S + G+L A GW +F Sbjct: 122 LGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFSLALFSPLLGWLTFAWGWEHVF 181 Query: 175 IIEGLPPIIWAFFWWKLVNDKPSETNWLTSQEKKEL-ESALHEEQKGLKPVKNYGEA--- 230 + G+ + W KL+++ P++ ++++E K + E+ + KP Sbjct: 182 TVMGVIGFVLTALWIKLIHN-PTDHPRMSAEELKFISENGAVVDMDHKKPGSAAASGPKL 240 Query: 231 --FRSPLVIRLCL-----QYFFWSIGVYGFVMWLPSIIKAAPDMSMVATGWLTSVPYVLA 283 + L R+ L QYF +I + F+ W P + MS++ G + S+P + Sbjct: 241 HYIKQLLSNRMMLGVFFGQYFINTI-TWFFLTWFPIYLVQEKGMSILKVGLVASIPALCG 299 Query: 284 VIGMLTASYFSDK--------TQNRKAFVWPFLMVGAVAFYASYLLGADHFWISFILLII 335 G + FSD T RK + +++ + +Y + +++++ Sbjct: 300 FAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLLASTIILCNYTNNT-----TLVVMLM 354 Query: 336 AGGAMYAPYGPF-FAIMPEILPRNVSGGAMALINSMGALGSFAGSYIVGYLNSFTGGFGA 394 A +G + ++ + P+ + G + N G + S ++GYL S F A Sbjct: 355 ALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPLVIGYLVSELHSFNA 414 Query: 395 SYIFMAGSLLLSAILTMTVGRSRKPAE 421 + +F+ S L++ + + V K E Sbjct: 415 ALVFVGCSALMAMVCYLFVVGDIKRME 441 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.325 0.138 0.435 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,802,267 Number of extensions: 1306242 Number of successful extensions: 4560 Number of sequences better than 1.0e-05: 38 Number of HSP's gapped: 4538 Number of HSP's successfully gapped: 39 Length of query: 433 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 324 Effective length of database: 25,807,025 Effective search space: 8361476100 Effective search space used: 8361476100 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 117 (49.7 bits)