BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0579.0014.p (254 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPy1255 [V] COG0577 ABC-type antimicrobial peptide transport sys... 70 4e-12 AF1820_1 [V] COG0577 ABC-type antimicrobial peptide transport sy... 59 8e-09 lin1187 [V] COG0577 ABC-type antimicrobial peptide transport sys... 54 2e-07 >SPy1255 [V] COG0577 ABC-type antimicrobial peptide transport system, permease component Length = 289 Score = 69.7 bits (169), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 12/150 (8%), Positives = 29/150 (19%), Gaps = 9/150 (6%) Query: 4 KLLFK-LLRDIKQSAGQFLSFVLVVAVGAFFYTGLAALSNNLGTYTEAYFKEYNLSDLNI 62 + + LL ++ Q LS V A + L + ++ + + + Sbjct: 4 AGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKDVTGHAIGAYIRARRLSKSA 63 Query: 63 YYS-QLTQKEIAELSKVEGIRKLEGRYTFDATESFDGYRAALKIHSIPENNEINRPAMTK 121 + + + + AL S Sbjct: 64 VALRLTARPILDIALQYRFDS-QQTFTRAFK--KQFAQTPALYRRSPEW--SAFGIRPPL 118 Query: 122 GSLPSTKDGIVLDSSYAKEHHYSVGDKIII 151 T + + Sbjct: 119 RLGEFTMPEHKFVTLEDT--PLIGVTQSYS 146 >AF1820_1 [V] COG0577 ABC-type antimicrobial peptide transport system, permease component Length = 289 Score = 58.9 bits (141), Expect = 8e-09, Method: Compositional matrix adjust. Identities = 10/115 (8%), Positives = 22/115 (19%) Query: 8 KLLRDIKQSAGQFLSFVLVVAVGAFFYTGLAALSNNLGTYTEAYFKEYNLSDLNIYYSQL 67 + F V Y LS + +++ + SQ Sbjct: 27 VAAKAGYSKWHLQRMFKDVTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQ 86 Query: 68 TQKEIAELSKVEGIRKLEGRYTFDATESFDGYRAALKIHSIPENNEINRPAMTKG 122 T + + + A R + + + Sbjct: 87 TFTRAFKKQFAQTPALYRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGV 141 >lin1187 [V] COG0577 ABC-type antimicrobial peptide transport system, permease component Length = 289 Score = 54.3 bits (129), Expect = 2e-07, Method: Compositional matrix adjust. Identities = 11/148 (7%), Positives = 31/148 (20%), Gaps = 7/148 (4%) Query: 11 RDIKQSAGQFLSFVLVVAVGAFFYTGLAALSNNLGTYTEAYFKEYNLSDLNI---YYSQL 67 ++ Q LS V A + L + ++ + + + + Sbjct: 12 IWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKDVTGHAIGAYIRARRLSKSAVALRLTAR 71 Query: 68 TQKEIAELSKVEGIRKLEGRYTFDATESFDGYRAALKIHSIPENNEINRPAMTKGSLPST 127 + + + F + + I P Sbjct: 72 PILD--IALQYRFDSQQTFTRAFKKQFAQTPA--LYRRSPEWSAFGIRPPLRLGEFTMPE 127 Query: 128 KDGIVLDSSYAKEHHYSVGDKIIIRVNN 155 + L+ + S + + Sbjct: 128 HKFVTLEDTPLIGVTQSYSCSLEQISDF 155 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.134 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,960,228 Number of extensions: 788232 Number of successful extensions: 1543 Number of sequences better than 1.0e-05: 5 Number of HSP's gapped: 1545 Number of HSP's successfully gapped: 5 Length of query: 254 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 150 Effective length of database: 26,383,695 Effective search space: 3957554250 Effective search space used: 3957554250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 114 (48.5 bits)