BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0579.0003.p (191 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yeeK [K] COG1309 Transcriptional regulator 110 1e-24 ECs1489 [K] COG1309 Transcriptional regulator 87 1e-17 STM1213 [K] COG1309 Transcriptional regulator 86 3e-17 AGc3874 [K] COG1309 Transcriptional regulator 77 2e-14 SMa1299 [K] COG1309 Transcriptional regulator 73 3e-13 >BS_yeeK [K] COG1309 Transcriptional regulator Length = 378 Score = 110 bits (276), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 65/193 (33%), Positives = 105/193 (54%), Gaps = 2/193 (1%) Query: 1 MGRYKEFDKEAVLHKAMLVFWKKGYEAASIPDLLEAMDLSRSSLYETFGDKQTLYIEAIN 60 +GR KEFD VLH+A+ VF + GYE S+ DLL + ++R SLY+T+G K+ L++ A+ Sbjct: 185 LGRNKEFDTALVLHRAIEVFGEYGYEGTSLQDLLSHLGIARQSLYDTYGTKRDLFLSAVK 244 Query: 61 WYKKWKQAK-RDILVNATSAKSGIRQYFEIHIDSALDGESPKGCLITNAAVGMDSPDEQL 119 Y + K A + L S K IR F+ +++ D E K C I N+A+ D +L Sbjct: 245 SYLEGKNAAVMERLEAPGSVKEAIRDIFQEGVNALRDPERAKACYIINSAIEQIPHDPEL 304 Query: 120 NALIRERFDELEKSFYELLRKGQKTGEIMPE-KDIKTLSYLLSNLNHSINIVSKVQGDRS 178 + +LE +FY L + + GE+ E DI L+ L+ S+ ++KV D Sbjct: 305 ARYFERQSKQLEDAFYHGLLRAKDQGELNGEDTDISALARYLNQSRLSLTFIAKVTADMD 364 Query: 179 KMQQMIDMVMEML 191 ++Q +D+ + +L Sbjct: 365 RLQDHVDVSLSVL 377 >ECs1489 [K] COG1309 Transcriptional regulator Length = 236 Score = 87.4 bits (215), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 50/191 (26%), Positives = 90/191 (47%), Gaps = 2/191 (1%) Query: 2 GRYKEFDKEAVLHKAMLVFWKKGYEAASIPDLLEAMDLSRSSLYETFGDKQTLYIEAINW 61 GR K FD++A L KAM +FW+ GYEA S+ DL+EA +LY F +K+ L+ ++ Sbjct: 40 GRPKVFDRDAALDKAMKLFWQHGYEATSLADLVEATGAKAPTLYAEFTNKEGLFRAVLDR 99 Query: 62 YKKWKQAKRD--ILVNATSAKSGIRQYFEIHIDSALDGESPKGCLITNAAVGMDSPDEQL 119 Y AK + + S +S + YF + ++P GC + N + + Sbjct: 100 YIDRFAAKHEAQLFCEEKSVESALADYFAAIANCFTGKDTPAGCFMINNCTTLSPDSGDI 159 Query: 120 NALIRERFDELEKSFYELLRKGQKTGEIMPEKDIKTLSYLLSNLNHSINIVSKVQGDRSK 179 ++ R E++ + L + Q GEI D+ L+ L+ + ++I ++ K Sbjct: 160 ANTLKSRHAMQERTLQQFLCQRQARGEIPTHCDVTHLAEFLNCIIQGMSISAREGASLEK 219 Query: 180 MQQMIDMVMEM 190 + Q+ + + Sbjct: 220 LMQIAGTTLRL 230 >STM1213 [K] COG1309 Transcriptional regulator Length = 211 Score = 86.3 bits (212), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 48/191 (25%), Positives = 93/191 (48%), Gaps = 2/191 (1%) Query: 2 GRYKEFDKEAVLHKAMLVFWKKGYEAASIPDLLEAMDLSRSSLYETFGDKQTLYIEAINW 61 GR K FD+EA L KAM +FW+ GYEA S+ DL+EA +LY F +K+ L+ ++ Sbjct: 14 GRPKVFDREAALDKAMTLFWQHGYEATSLADLVEATGAKAPTLYAEFTNKKGLFRAVLDR 73 Query: 62 YKKWKQAKRD--ILVNATSAKSGIRQYFEIHIDSALDGESPKGCLITNAAVGMDSPDEQL 119 Y +K + + S +S + YF ++P GC + N + + + + Sbjct: 74 YITRFASKHEAQLFCEEKSVESALEDYFTEIAACFTSTDTPAGCFMINTSATLAASSRDI 133 Query: 120 NALIRERFDELEKSFYELLRKGQKTGEIMPEKDIKTLSYLLSNLNHSINIVSKVQGDRSK 179 ++ R E++ + LR+ Q+ GEI + + L+ ++ + ++I ++ + Sbjct: 134 ARTVKSRHAMQEQTLIQFLRQRQERGEIPAHCNPQALAEYINCILQGMSISAREGATFEQ 193 Query: 180 MQQMIDMVMEM 190 + Q+ + + Sbjct: 194 LMQITRTTLRI 204 >AGc3874 [K] COG1309 Transcriptional regulator Length = 289 Score = 77.0 bits (188), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 9/190 (4%), Positives = 26/190 (13%), Gaps = 1/190 (0%) Query: 2 GRYKEFDKEAVLHKAMLVFWKKGYEAASIPDLLEAMDLSRSSLYETFGDKQTLYIEAINW 61 +F K K G A Sbjct: 49 VSPDDFPKFIATLKEGKPGAAVGGNATIPHKEAAYRLADHPDALAEELGAANTIWMEEGK 108 Query: 62 YKKWKQAKRDILV-NATSAKSGIRQYFEIHIDSALDGESPKGCLITNAAVGMDSPDEQLN 120 + + + + + L + + + Sbjct: 109 LHATNTDGYGFVSNLDERHPGWDKTQRAVVFGAGGASRAVIQSLRDRDVAEIHVVNRTVE 168 Query: 121 ALIRERFDELEKSFYELLRKGQKTGEIMPEKDIKTLSYLLSNLNHSINIVSKVQGDRSKM 180 + F Q+ T + ++ Sbjct: 169 RARELADRFGPRVFSHPQAALQEVMHGAGLFVNTTSLGMNGTEAPHLDFSHLAANAVVTD 228 Query: 181 QQMIDMVMEM 190 + + + Sbjct: 229 IVYVPLKTPI 238 >SMa1299 [K] COG1309 Transcriptional regulator Length = 203 Score = 72.8 bits (177), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 51/195 (26%), Positives = 90/195 (46%), Gaps = 7/195 (3%) Query: 1 MGRYKEFDKEAVLHKAMLVFWKKGYEAASIPDLLEAMDLSRSSLYETFGDKQTLYIEAIN 60 +GR +EFD L A+ +F +KGY SI +L M L+ S+Y+ F DK+ +++ A Sbjct: 11 VGRPREFDINQALDAAIRIFSEKGYHGTSIAELKTEMGLTAGSIYKAFRDKRDVFVAA-- 68 Query: 61 WYKKWKQAKRDI----LVNATSAKSGIRQYFEIHIDSALDGESPKGCLITNAAVGMDSPD 116 Y ++KQ + D+ L +A++ + I + + SA +GCL AAV + D Sbjct: 69 -YDRYKQIRADLLDQALFSASTGREKIARVVGFYAMSACGETGRRGCLAIGAAVELVLSD 127 Query: 117 EQLNALIRERFDELEKSFYELLRKGQKTGEIMPEKDIKTLSYLLSNLNHSINIVSKVQGD 176 ++ + +L +R GQ G I + D + L + + I K Sbjct: 128 AEIADRVGRHNRKLVARLEGFIRDGQLDGSIRQDVDPAATALALFSYLQGVRIAGKTAEL 187 Query: 177 RSKMQQMIDMVMEML 191 ++M + V+ +L Sbjct: 188 ENQMLPSAEAVLRIL 202 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.133 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 13,380,796 Number of extensions: 539035 Number of successful extensions: 1224 Number of sequences better than 1.0e-05: 6 Number of HSP's gapped: 1223 Number of HSP's successfully gapped: 6 Length of query: 191 Length of database: 38,378,431 Length adjustment: 100 Effective length of query: 91 Effective length of database: 26,845,031 Effective search space: 2442897821 Effective search space used: 2442897821 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 113 (48.1 bits)