BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0578.0010.p (273 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0305 [MG] COG0702 Predicted nucleoside-diphosphate-sugar epime... 150 2e-36 ytfG [MG] COG0702 Predicted nucleoside-diphosphate-sugar epimerases 147 2e-35 ZytfG [MG] COG0702 Predicted nucleoside-diphosphate-sugar epimer... 147 2e-35 ECs5190 [MG] COG0702 Predicted nucleoside-diphosphate-sugar epim... 145 6e-35 >BH0305 [MG] COG0702 Predicted nucleoside-diphosphate-sugar epimerases Length = 284 Score = 150 bits (378), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 104/288 (36%), Positives = 152/288 (52%), Gaps = 25/288 (8%) Query: 1 MTIMITGAAGQLGNLIIENLLAELSDGTIIAGVRNLDTATHFKDKGVVVRCADYDRPETL 60 M +++TGA GQLG+L++E LL + + VR+ A H K +GV VR D+ +PE+L Sbjct: 1 MKLLVTGATGQLGSLVVEALLKTVPAENVAVSVRDPKKAEHLKAQGVDVRQGDFTQPESL 60 Query: 61 QDAFAGVNKLLLISSSHSDDAVRLTQHTNVIDAAKAAGVAHILYTSFAFPQAGNKGTSSV 120 AFAGV+K+L+ISS+ D R+ QH I AAK V I YTS A Q + Sbjct: 61 VSAFAGVDKILIISSAPGD---RVAQHKAAIQAAKENNVRFIAYTSIANAQDNPFFIAED 117 Query: 121 HGLTEQAILDSGMEYTILRNGLYIDFVGVLGLNEAIRSGV-LATQPGDWQFNAVTRRDLA 179 H TE+AI++SG+ Y+ LRN Y++ +G +A+ SG T G + RRD A Sbjct: 118 HRETEKAIVESGIPYSFLRNNWYLE--NEVGTIQAVASGAPWLTSAGSGKVGWAPRRDYA 175 Query: 180 RATAKVLAGSGHEHRIYELAAAQTWTFAGLAEVLTELAGKPV-IHTAD----------AS 228 A A VL G GHEH YEL + T LA+V+++ G+ V + D A Sbjct: 176 DAAAAVLTGEGHEHTTYEL-SGHPLTQEELAQVVSDALGRDVEVQQVDDATYEQAMSGAG 234 Query: 229 VQHWIYHFLNSIDT-------KSTSNDLEQLMGQPVTSLKESIAPFLN 269 + + F+ I S D E+++G+P+T LK+++ LN Sbjct: 235 IPEEVLPFIVGIQQGIREGYLNVVSGDFEKVLGRPLTPLKDAVREILN 282 >ytfG [MG] COG0702 Predicted nucleoside-diphosphate-sugar epimerases Length = 286 Score = 147 bits (370), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 110/288 (38%), Positives = 148/288 (51%), Gaps = 21/288 (7%) Query: 3 IMITGAAGQLGNLIIENLLAELSDGTIIAGVRNLDTATHFKDKGVVVRCADYDRPETLQD 62 I ITGA GQLG+ +IE+L+ + I+A VRN A +G+ VR ADY L Sbjct: 2 IAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQGITVRQADYGDEAALTS 61 Query: 63 AFAGVNKLLLISSSHSDDAVRLTQHTNVIDAAKAAGVAHILYTSFAFPQAGNKGTSSVHG 122 A GV KLLLISSS R QH NVI+AAKAAGV I YTS G + H Sbjct: 62 ALQGVEKLLLISSSEVGQ--RAPQHRNVINAAKAAGVKFIAYTSLLHADTSPLGLADEHI 119 Query: 123 LTEQAILDSGMEYTILRNGLYIDFVGVLGLNEAIRSGVLATQPGDWQFNAVTRRDLARAT 182 TE+ + DSG+ YT+LRNG Y + + A+ GV GD + + TR D A A Sbjct: 120 ETEKMLADSGIVYTLLRNGWYSENY-LASAPAALEHGVFIGAAGDGKIASATRADYAAAA 178 Query: 183 AKVLAGSGHEHRIYELAAAQTWTFAGLAEVLTELAGKPVIHT----AD-------ASVQH 231 A+V++ +GHE ++YELA WT LA LT+ +GK V + AD + Sbjct: 179 ARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVTYQNLSEADFAAALKSVGLPD 238 Query: 232 WIYHFLNSIDTKST-------SNDLEQLMGQPVTSLKESIAPFLNLEN 272 + L D ++ S L +L+G P T+L ES++ N+ N Sbjct: 239 GLADMLADSDVGASKGGLFDDSKTLSKLIGHPTTTLAESVSHLFNVNN 286 >ZytfG [MG] COG0702 Predicted nucleoside-diphosphate-sugar epimerases Length = 286 Score = 147 bits (370), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 110/288 (38%), Positives = 148/288 (51%), Gaps = 21/288 (7%) Query: 3 IMITGAAGQLGNLIIENLLAELSDGTIIAGVRNLDTATHFKDKGVVVRCADYDRPETLQD 62 I ITGA GQLG+ +IE+L+ + I+A VRN A +G+ VR ADY L Sbjct: 2 IAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQGITVRQADYGDEAALTS 61 Query: 63 AFAGVNKLLLISSSHSDDAVRLTQHTNVIDAAKAAGVAHILYTSFAFPQAGNKGTSSVHG 122 A GV KLLLISSS R QH NVI+AAKAAGV I YTS G + H Sbjct: 62 ALQGVEKLLLISSSEVGQ--RAPQHRNVINAAKAAGVKFIAYTSLLHADTSPLGLADEHI 119 Query: 123 LTEQAILDSGMEYTILRNGLYIDFVGVLGLNEAIRSGVLATQPGDWQFNAVTRRDLARAT 182 TE+ + DSG+ YT+LRNG Y + + A+ GV GD + + TR D A A Sbjct: 120 ETEKMLADSGIVYTLLRNGWYSENY-LASAPAALEHGVFIGAAGDGKIASATRADYAAAA 178 Query: 183 AKVLAGSGHEHRIYELAAAQTWTFAGLAEVLTELAGKPVIHT----AD-------ASVQH 231 A+V++ +GHE ++YELA WT LA LT+ +GK V + AD + Sbjct: 179 ARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVTYQNLSEADFAAALKSVGLPD 238 Query: 232 WIYHFLNSIDTKST-------SNDLEQLMGQPVTSLKESIAPFLNLEN 272 + L D ++ S L +L+G P T+L ES++ N+ N Sbjct: 239 GLADMLADSDVGASKGGLFDDSKTLSKLIGHPTTTLAESVSHLFNVNN 286 Score = 145 bits (366), Expect = 6e-35, Method: Compositional matrix adjust. Identities = 110/288 (38%), Positives = 148/288 (51%), Gaps = 21/288 (7%) Query: 3 IMITGAAGQLGNLIIENLLAELSDGTIIAGVRNLDTATHFKDKGVVVRCADYDRPETLQD 62 I ITGA GQLG+ +IE+L+ + I+A VRN A +G+ VR ADY L Sbjct: 2 IAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQGITVRQADYGDEAALTS 61 Query: 63 AFAGVNKLLLISSSHSDDAVRLTQHTNVIDAAKAAGVAHILYTSFAFPQAGNKGTSSVHG 122 A GV KLLLISSS R QH NVI+AAKAAGV I YTS G + H Sbjct: 62 ALQGVEKLLLISSSEVGQ--RAPQHRNVINAAKAAGVKFIAYTSLLHADTSPLGLADEHI 119 Query: 123 LTEQAILDSGMEYTILRNGLYIDFVGVLGLNEAIRSGVLATQPGDWQFNAVTRRDLARAT 182 TE+ + DSG+ YT+LRNG Y + + A+ GV GD + + TR D A A Sbjct: 120 ETEKMLADSGIVYTLLRNGWYSENY-LASAPAALEHGVFIGAAGDGKIASATRADYAAAA 178 Query: 183 AKVLAGSGHEHRIYELAAAQTWTFAGLAEVLTELAGKPVIHT----AD-------ASVQH 231 A+V++ +GHE ++YELA WT LA LT+ +GK V + AD + Sbjct: 179 ARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVTYQNLSEADFAAALKSVGLPD 238 Query: 232 WIYHFLNSIDTKST-------SNDLEQLMGQPVTSLKESIAPFLNLEN 272 + L D ++ S L +L+G P T+L ES++ N+ N Sbjct: 239 GLADMLADSDVGASKGGLFDDSKTLSKLIGHPTTTLAESVSHLFNVNN 286 >ECs5190 [MG] COG0702 Predicted nucleoside-diphosphate-sugar epimerases Length = 286 Score = 145 bits (366), Expect = 6e-35, Method: Compositional matrix adjust. Identities = 109/288 (37%), Positives = 146/288 (50%), Gaps = 21/288 (7%) Query: 3 IMITGAAGQLGNLIIENLLAELSDGTIIAGVRNLDTATHFKDKGVVVRCADYDRPETLQD 62 I ITGA GQLG +IE+L+ + I+A VRN A +G+ VR ADY L Sbjct: 2 IAITGATGQLGQYVIESLMKTVPASQIVAIVRNPAKAQALTAQGITVRQADYGDEAALTS 61 Query: 63 AFAGVNKLLLISSSHSDDAVRLTQHTNVIDAAKAAGVAHILYTSFAFPQAGNKGTSSVHG 122 A GV KLLLISSS R QH NVI+AAK AGV I YTS G + H Sbjct: 62 ALQGVEKLLLISSSEVGQ--RAPQHRNVINAAKTAGVKFIAYTSLLHADKSPLGLADEHI 119 Query: 123 LTEQAILDSGMEYTILRNGLYIDFVGVLGLNEAIRSGVLATQPGDWQFNAVTRRDLARAT 182 TE+ + DSG+ YT+LRNG Y + + A+ GV GD + + TR D A A Sbjct: 120 ETEKMLADSGIVYTLLRNGWYTENY-LASAPAALEHGVFIGAAGDGKIASATRADYAAAA 178 Query: 183 AKVLAGSGHEHRIYELAAAQTWTFAGLAEVLTELAGKPVIHT----AD-------ASVQH 231 A+V++ +GHE ++YELA WT LA LT+ +GK V + AD + Sbjct: 179 ARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVTYQNLSEADFAAALKSVGLPD 238 Query: 232 WIYHFLNSIDTKST-------SNDLEQLMGQPVTSLKESIAPFLNLEN 272 + L D ++ S L +L+G P T+L ES++ N+ N Sbjct: 239 GLADMLADSDVGASKGGLFDDSKTLSKLIGHPTTTLAESVSHLFNVNN 286 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.132 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,170,970 Number of extensions: 812445 Number of successful extensions: 2306 Number of sequences better than 1.0e-05: 25 Number of HSP's gapped: 2266 Number of HSP's successfully gapped: 25 Length of query: 273 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 168 Effective length of database: 26,268,361 Effective search space: 4413084648 Effective search space used: 4413084648 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)