BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0576.0006.p (632 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0296_2 [G] COG1263 Phosphotransferase system IIC components, g... 228 3e-59 BS_bglP_2 [G] COG1263 Phosphotransferase system IIC components, ... 204 4e-52 lin0026_2 [G] COG1263 Phosphotransferase system IIC components, ... 196 9e-50 BH0595_2 [G] COG1263 Phosphotransferase system IIC components, g... 177 4e-44 bglF_2 [G] COG1263 Phosphotransferase system IIC components, glu... 176 1e-43 >BH0296_2 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 368 Score = 228 bits (580), Expect = 3e-59, Method: Compositional matrix adjust. Identities = 124/281 (44%), Positives = 176/281 (62%), Gaps = 2/281 (0%) Query: 100 MSRIFAVISGSLSPLLPAMAGAGILKAFLTLFTTFGWISAESGTYLILAAAANAVFYFLP 159 +SR+ V++ +PLL MAGAGILK L + T GW+ + TY+IL AA++++FYFLP Sbjct: 1 VSRLIDVVASIFTPLLGVMAGAGILKGLLAIATNVGWLDSGETTYIILHAASDSMFYFLP 60 Query: 160 IFLGVSASFKFGANPYVGGVIGAALMEPNFTGLLANGDTSSFLGIPVVLMDYSSTVFPVF 219 I L V+A+ KF NP+ I AL+ P+ L + +F GIPVVLM+Y+STVFP+ Sbjct: 61 ILLAVTAARKFETNPFGAATIAGALIYPSIVELHDSAIDVTFFGIPVVLMNYTSTVFPIL 120 Query: 220 VAIWIFSYVEKFLKKIIHKELQTFMXXXXXXXXXXXXTAMIFGPFGVYIGNAIADAINYL 279 +A++ SYVEKF K IH+ ++ F+ T +I GP GVY+GN IA I + Sbjct: 121 LAVFAMSYVEKFCNKKIHEAVKNFVTPLILLVVIVPVTLIILGPIGVYLGNGIASVIQEI 180 Query: 280 MGVSGLLTGAVIGGLIFFLVVFGLHWGIIPIVLANLA-NGTDPILAMWAASNFAMAGVAL 338 S +L GA++ G+ LV+FG+HWGIIPI+L NL+ G D I A+ A + F+ AG AL Sbjct: 181 FTFSPVLAGAIVAGIWQVLVIFGIHWGIIPIILNNLSVRGEDVIKAVAAPAVFSQAGAAL 240 Query: 339 AVFLKSKEKSLKSIAGXXXXXXXXAGVSEPTVYGILLRHKR 379 V L++K K LK++AG G++EP VYG+ L K+ Sbjct: 241 GVMLRTKNKKLKALAGSTSITALF-GITEPAVYGVTLPLKK 280 >BS_bglP_2 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 364 Score = 204 bits (519), Expect = 4e-52, Method: Compositional matrix adjust. Identities = 109/285 (38%), Positives = 171/285 (60%), Gaps = 2/285 (0%) Query: 96 KEKMMSRIFAVISGSLSPLLPAMAGAGILKAFLTLFTTFGWISAESGTYLILAAAANAVF 155 K+ ++S +F VISG +P+LPA+AGAG++K + L TFGW++ +S ++IL A + F Sbjct: 4 KKNVLSAVFDVISGVFTPILPAIAGAGMIKGLVALAVTFGWMAEKSQVHVILTAVGDGAF 63 Query: 156 YFLPIFLGVSASFKFGANPYVGGVIGAALMEPNFTGLLANGDTSSFLGIPVVLMDYSSTV 215 YFLP+ L +SA+ KFG+NPYV I AA++ P+ T LL G SF+G+PV YSSTV Sbjct: 64 YFLPLLLAMSAARKFGSNPYVAAAIAAAILHPDLTALLGAGKPISFIGLPVTAATYSSTV 123 Query: 216 FPVFVAIWIFSYVEKFLKKIIHKELQTFMXXXXXXXXXXXXTAMIFGPFGVYIGNAIADA 275 P+ ++IWI SYVEK++ + H L+ + T + GP G +G ++ Sbjct: 124 IPILLSIWIASYVEKWIDRFTHASLKLIVVPTFTLLIVVPLTLITVGPLGAILGEYLSSG 183 Query: 276 INYLMGVSGLLTGAVIGGLIFFLVVFGLHWGIIPIVLANLA-NGTDPILAMWAASNFAMA 334 +NYL +GL+ + G +++ G+H+ +PI++ N+A NG D +L +N A Sbjct: 184 VNYLFDHAGLVAMIFLAGTFSLIIMTGMHYAFVPIMINNIAQNGHDYLLPAMFLANMGQA 243 Query: 335 GVALAVFLKSKEKSLKSIAGXXXXXXXXAGVSEPTVYGILLRHKR 379 G + AVFL+S+ K KS+A G++EP +YG+ +R K+ Sbjct: 244 GASFAVFLRSRNKKFKSLA-LTTSITALMGITEPAMYGVNMRLKK 287 >lin0026_2 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 399 Score = 196 bits (498), Expect = 9e-50, Method: Compositional matrix adjust. Identities = 114/307 (37%), Positives = 172/307 (56%), Gaps = 19/307 (6%) Query: 90 EDANQPKE-KMMSRIFAVISGSLSPLLPAMAGAGILKAFLTLFTTFGWISAESGTYLILA 148 +D+N P + +R +ISG +P+L +A G++K F +F FGWI+ SGTY +L Sbjct: 4 DDSNVPASGNIFNRFIDMISGVFTPVLGVLAATGMIKGFTAMFAAFGWITVTSGTYQLLY 63 Query: 149 AAANAVFYFLPIFLGVSASFKFGANPYVGGVIGAALMEPNFTGLLANGDT---------- 198 A + +FYF PIFLG +A KFG N ++G IG AL+ P G+ A GD Sbjct: 64 AIGDCLFYFFPIFLGFTAMKKFGGNIFIGMAIGGALVYPTLAGISA-GDPIYTLFAGTIF 122 Query: 199 -----SSFLGIPVVLMDYSSTVFPVFVAIWIFSYVEKFLKKIIHKELQTFMXXXXXXXXX 253 +FLGIPV+LM Y+S+V P+ +A + S VEK KKII ++TF+ Sbjct: 123 ESPIHVTFLGIPVILMSYASSVIPIILATYFGSKVEKGFKKIIPDVIKTFVVPFCTLLVV 182 Query: 254 XXXTAMIFGPFGVYIGNAIADAINYLMGVSGLLTGAVIGGLIFFLVVFGLHWGIIPIVLA 313 T ++ GP + G + ++ +S ++ G ++GG V+FGLHWG+IP+ + Sbjct: 183 VPITFLVIGPIATWAGQLLGAGTIWVYNLSPVVAGLILGGFWQVFVIFGLHWGLIPVAIN 242 Query: 314 NLAN-GTDPILAMWAASNFAMAGVALAVFLKSKEKSLKSIAGXXXXXXXXAGVSEPTVYG 372 NL G+DP+LAM ++FA G LAVF K++ K +KS++ GV+EP +YG Sbjct: 243 NLTTLGSDPVLAMMFGASFAQIGAVLAVFFKTRNKKIKSLS-IPAFISGIFGVTEPAIYG 301 Query: 373 ILLRHKR 379 + L K+ Sbjct: 302 VTLPLKK 308 >BH0595_2 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 399 Score = 177 bits (450), Expect = 4e-44, Method: Compositional matrix adjust. Identities = 106/309 (34%), Positives = 173/309 (55%), Gaps = 17/309 (5%) Query: 86 NSASEDANQPKEKMMSRIFAVISGSLSPLLPAMAGAGILKAFLTLFTTFGWISAESGTYL 145 + +ED + + + ++ +I+ +P+L +A G++K LF + G + +GTY Sbjct: 2 ETETEDEKK-YQGLFNKFIDIIASIFTPVLGVLAATGMIKGLNALFLSTGVLEEANGTYQ 60 Query: 146 ILAAAANAVFYFLPIFLGVSASFKFGANPYVGGVIGAALMEPN-------------FTGL 192 +L A +++FYF PIFLG +A+ KFGA P++G IGA+L+ P FTG Sbjct: 61 LLHAIGDSLFYFFPIFLGYTAAKKFGATPFIGMAIGASLVYPTLVVLTEGEPLYTLFTGT 120 Query: 193 LANGDTS-SFLGIPVVLMDYSSTVFPVFVAIWIFSYVEKFLKKIIHKELQTFMXXXXXXX 251 + +FLGIPV+LM Y+++V P+ +A + S VE L+KII ++TF+ Sbjct: 121 IFESPVHITFLGIPVILMSYATSVIPIILAAYFASKVEARLRKIIPDVVKTFLVPFFTLL 180 Query: 252 XXXXXTAMIFGPFGVYIGNAIADAINYLMGVSGLLTGAVIGGLIFFLVVFGLHWGIIPIV 311 T ++ GP + G + ++ +S ++ GA +GG V+FGLHWG+IPI Sbjct: 181 IVVPLTFIVIGPIATWAGQLLGQFTLWVYNLSPIIAGAFLGGFWQVFVIFGLHWGLIPIA 240 Query: 312 LANL-ANGTDPILAMWAASNFAMAGVALAVFLKSKEKSLKSIAGXXXXXXXXAGVSEPTV 370 + NL G+DP+LAM A++FA G AV+LK K++ +K+++ GV+EP + Sbjct: 241 INNLVVQGSDPVLAMVFAASFAQIGAVAAVWLKIKQQKVKTLS-VPAFISGIFGVTEPAI 299 Query: 371 YGILLRHKR 379 YG+ L KR Sbjct: 300 YGVTLPLKR 308 >bglF_2 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 361 Score = 176 bits (445), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 105/289 (36%), Positives = 153/289 (52%), Gaps = 7/289 (2%) Query: 96 KEKMMSRIFAVISGSLSPLLPAMAGAGILKAFLTLFTTFGWISAESGTYLILAAAANAVF 155 K +++R VISG +PL+ MA GILK L L TF W + +SGTYLIL +A++A+F Sbjct: 3 KGNLLNRFVYVISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALF 62 Query: 156 YFLPIFLGVSASFKFGANPYVGGVIGAALMEPNFTGLLANGDTS-----SFLGIPVVLMD 210 +F PI LG +A +FG NP+ VIG AL+ P NG + FLGIPV L++ Sbjct: 63 WFFPIILGYTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLN 122 Query: 211 YSSTVFPVFVAIWIFSYVEKFLKKIIHKELQTFMXXXXXXXXXXXXTAMIFGPFGVYIGN 270 YSS+V P+ + W+ S +E+ L + ++ F T ++ GP +I Sbjct: 123 YSSSVIPIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISE 182 Query: 271 AIADAINYLMGVSGLLTGAVIGGLIFFLVVFGLHWGIIPIVLANLAN-GTDPILAMWAAS 329 IA +L GAV+GG V+FGLHWG++P+ + N G D ++ + + Sbjct: 183 LIAAGYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPA 242 Query: 330 NFAMAGVALAVFLKSKEKSLKSIAGXXXXXXXXAGVSEPTVYGILLRHK 378 A G AL VFL ++ K +AG G++EP VYG+ L K Sbjct: 243 IMAQVGAALGVFLCERDAQKKVVAGSAALTSLF-GITEPAVYGVNLPRK 290 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.137 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 42,629,365 Number of extensions: 1871869 Number of successful extensions: 9605 Number of sequences better than 1.0e-05: 140 Number of HSP's gapped: 9492 Number of HSP's successfully gapped: 141 Length of query: 632 Length of database: 38,378,431 Length adjustment: 113 Effective length of query: 519 Effective length of database: 25,345,689 Effective search space: 13154412591 Effective search space used: 13154412591 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 119 (50.4 bits)