BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0574.0027.p (207 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ydfN [C] COG0778 Nitroreductase 249 2e-66 CAC0850 [C] COG0778 Nitroreductase 226 2e-59 BH2236 [C] COG0778 Nitroreductase 134 1e-31 BS_yodC [C] COG0778 Nitroreductase 129 3e-30 PA5190 [C] COG0778 Nitroreductase 106 3e-23 >BS_ydfN [C] COG0778 Nitroreductase Length = 346 Score = 249 bits (637), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 9/205 (4%), Positives = 32/205 (15%), Gaps = 1/205 (0%) Query: 3 MSTFSELVQARRSANNFVEGIKIPQSELEEMFSLTRLAPSAYNLQHTHYKVVSDEDLKEK 62 M + + + + + E A + + + + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 63 IREAAYGQYKIHTASAVIVVLGDKNAYLQASEIYSGLKMLGAMSEDAFDQTIADIHGAYS 122 + A + + + + + D + A Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 123 GN-DAFQHDEAIRNASLSAMQFMLIAKDKGWDTCPMIGFDVQAVKAALNLSDNMVPVMLI 181 D + + L + + A+ + Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 182 TIGKDNKHKIRPRGYRKPVNEFVEF 206 + Sbjct: 181 ETAFGVMRYLHLADKHDLPRIVTIQ 205 >CAC0850 [C] COG0778 Nitroreductase Length = 346 Score = 226 bits (576), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 9/205 (4%), Positives = 32/205 (15%), Gaps = 1/205 (0%) Query: 3 MSTFSELVQARRSANNFVEGIKIPQSELEEMFSLTRLAPSAYNLQHTHYKVVSDEDLKEK 62 M + + + + + E A + + + + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 63 IREAAYGQYKIHTASAVIVVLGDKNAYLQASEIYSGLKMLGAMSEDAFDQTIADIHGAYS 122 + A + + + + + D + A Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 123 GN-DAFQHDEAIRNASLSAMQFMLIAKDKGWDTCPMIGFDVQAVKAALNLSDNMVPVMLI 181 D + + L + + A+ + Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 182 TIGKDNKHKIRPRGYRKPVNEFVEF 206 + Sbjct: 181 ETAFGVMRYLHLADKHDLPRIVTIQ 205 >BH2236 [C] COG0778 Nitroreductase Length = 346 Score = 134 bits (336), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 10/181 (5%), Positives = 26/181 (14%) Query: 8 ELVQARRSANNFVEGIKIPQSELEEMFSLTRLAPSAYNLQHTHYKVVSDEDLKEKIREAA 67 + ++L+ + + + + + + Sbjct: 14 STLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWL 73 Query: 68 YGQYKIHTASAVIVVLGDKNAYLQASEIYSGLKMLGAMSEDAFDQTIADIHGAYSGNDAF 127 V G + L + Sbjct: 74 AKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHW 133 Query: 128 QHDEAIRNASLSAMQFMLIAKDKGWDTCPMIGFDVQAVKAALNLSDNMVPVMLITIGKDN 187 L DT + +A K N ++ Sbjct: 134 PQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLA 193 Query: 188 K 188 Sbjct: 194 D 194 >BS_yodC [C] COG0778 Nitroreductase Length = 346 Score = 129 bits (325), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 10/183 (5%), Positives = 33/183 (18%), Gaps = 1/183 (0%) Query: 3 MSTFSELVQARRSANNFVEGIKIPQSELEEMFSLTRLAPSAYNLQHTHYKVVSDEDLKEK 62 M + + + + + E A + + + + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 63 IREAAYGQYKIHTASAVIVVLGDKNAYLQASEIYSGLKMLGAMSEDAFDQTIADIHGAYS 122 + A + + + + + D + A Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 123 GNDAFQHDEAIRNASLSAMQFMLIAKDKGWDTCPMIGFDVQAVKAALNLSDNMVPVMLIT 182 D + D+ P + A + + ++ Sbjct: 121 -LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVS 179 Query: 183 IGK 185 Sbjct: 180 NET 182 >PA5190 [C] COG0778 Nitroreductase Length = 346 Score = 106 bits (264), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 9/196 (4%), Positives = 28/196 (14%), Gaps = 8/196 (4%) Query: 10 VQARRSANNFVEGIKIPQSELEEMFSLTRLAPSAYNLQHTHYKVVSDEDLKEKIREAAYG 69 + + G + E + +L + + + Sbjct: 7 PHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTE 66 Query: 70 QYKIHTASAVIVVLGDKNAYLQ-ASEIYSGLKMLGAMSEDAFDQTIADIHGAYSGNDAFQ 128 Y + + A + + D + + Sbjct: 67 TYVGNWLAKHGSREKLIIASKVSGPSRNND----KGIRPDQALDRKNIRE--ALHDSLKR 120 Query: 129 HDEAIRNASLSAMQFMLIAKDKGWDTCPMIGFDVQAVKAALNLSDNMVPVMLITIGKDNK 188 + ++ L+ I + Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 189 HKIRPRGYRKPVNEFV 204 R Sbjct: 181 ETA-FGVMRYLHLADK 195 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.132 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 14,216,569 Number of extensions: 572153 Number of successful extensions: 1137 Number of sequences better than 1.0e-05: 30 Number of HSP's gapped: 1118 Number of HSP's successfully gapped: 30 Length of query: 207 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 105 Effective length of database: 26,614,363 Effective search space: 2794508115 Effective search space used: 2794508115 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 113 (48.1 bits)