BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0574.0025.p (392 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yjlD [C] COG1252 NADH dehydrogenase, FAD-containing subunit 617 e-177 SA0802 [C] COG1252 NADH dehydrogenase, FAD-containing subunit 217 3e-56 BH3407 [C] COG1252 NADH dehydrogenase, FAD-containing subunit 213 5e-55 BS_yumB [C] COG1252 NADH dehydrogenase, FAD-containing subunit 211 2e-54 lin2488 [C] COG1252 NADH dehydrogenase, FAD-containing subunit 196 5e-50 >BS_yjlD [C] COG1252 NADH dehydrogenase, FAD-containing subunit Length = 392 Score = 617 bits (1591), Expect = e-177, Method: Compositional matrix adjust. Identities = 299/392 (76%), Positives = 334/392 (85%) Query: 1 MSKHIVILGAGYGGLLSAINVRKYMSKAEAKITVVNQYPTHQIITELHRLAAGSVAEQAV 60 MSKHIVILGAGYGG+LSA+ VRK+ +K +A++TVVN+YPTHQIITELHRLAAG+V+E+AV Sbjct: 1 MSKHIVILGAGYGGVLSALTVRKHYTKEQARVTVVNKYPTHQIITELHRLAAGNVSEKAV 60 Query: 61 AMPLTKLFAGKDIDLKIAKVKSFSVDSKQVQLSDGTTLSYDALVVALGSTTAYFGIPGLE 120 AMPL KLF GKDIDLKIA+V SFSVD K+V L+DG+TL+YDALVV LGS TAYFGIPGLE Sbjct: 61 AMPLEKLFKGKDIDLKIAEVSSFSVDKKEVALADGSTLTYDALVVGLGSVTAYFGIPGLE 120 Query: 121 EYSMVLKSAADAKQIHGHIEGRIREYAKTHNPADAAXXXXXXXXXXXXXXXXXADVLPKL 180 E SMVLKSAADA ++ H+E R+REY+KT N ADA AD++P L Sbjct: 121 ENSMVLKSAADANKVFQHVEDRVREYSKTKNEADATILIGGGGLTGVELVGELADIMPNL 180 Query: 181 AKRYGVNPQEIKLMLVEAGPKILPVLPDHLIERAVTSLTARGVTFLTGLPVTNVAGNVID 240 AK+YGV+ +EIKL LVEAGPKILPVLPD LIERA SL RGV FLTGLPVTNV GNVID Sbjct: 181 AKKYGVDHKEIKLKLVEAGPKILPVLPDDLIERATASLEKRGVEFLTGLPVTNVEGNVID 240 Query: 241 LKDGQQIVANTFVWTGGVQGNPLVGESGLEVNRGRATVNDFLQSTSHPDVFVAGDSAVVF 300 LKDG ++VANTFVWTGGVQGNPLVGESGLEVNRGRATVNDFLQSTSH DVFVAGDSAV F Sbjct: 241 LKDGSKVVANTFVWTGGVQGNPLVGESGLEVNRGRATVNDFLQSTSHEDVFVAGDSAVYF 300 Query: 301 AADGRPYPPTAQIAWQMGELIGYNLYAYLTNAASEAFSPINSGTLASLGRKDGVAIIGAS 360 DGRPYPPTAQIAWQMGELIGYNL+AYL E F P+NSGTLASLGRKD VAIIGA+ Sbjct: 301 GPDGRPYPPTAQIAWQMGELIGYNLFAYLEGKTLETFKPVNSGTLASLGRKDAVAIIGAN 360 Query: 361 NTPLKGLPATLMKEASNIRYLSHIKGLFSLAY 392 +TPLKGLPA+LMKEASN+RYL+HIKGLFSLAY Sbjct: 361 STPLKGLPASLMKEASNVRYLTHIKGLFSLAY 392 >SA0802 [C] COG1252 NADH dehydrogenase, FAD-containing subunit Length = 402 Score = 217 bits (553), Expect = 3e-56, Method: Compositional matrix adjust. Identities = 131/392 (33%), Positives = 210/392 (53%), Gaps = 11/392 (2%) Query: 3 KHIVILGAGYGGLLSAINVRKYMSKAEAKITVVNQYPTHQIITELHRLAAGSVAEQAVAM 62 K +++LGAGY GL + ++K +S EA+IT++N+ H T LH +AG++ + V Sbjct: 6 KKVLVLGAGYAGLQTVTKLQKAISTEEAEITLINKNEYHYEATWLHEASAGTLNYEDVLY 65 Query: 63 PLTKLFAGKDIDLKIAKVKSFSVDSKQVQLSDGTTLSYDALVVALGSTTAYFGIPGLEEY 122 P+ + ++ A+V D+K+V+ + G +D LVVALG + FGI G++++ Sbjct: 66 PVESVLKKDKVNFVQAEVTKIDRDAKKVETNQG-IYDFDILVVALGFVSETFGIEGMKDH 124 Query: 123 SMVLKSAADAKQIHGHIEGRIREYA--KTHNPADAAXXXXXXXXXXXXXXXXXADVLPKL 180 + +++ A+++ HIE + YA K + D + D +P+L Sbjct: 125 AFQIENVITARELSRHIEDKFANYAASKEKDDNDLSILVGGAGFTGVEFLGELTDRIPEL 184 Query: 181 AKRYGVNPQEIKLMLVEAGPKILPVLPDHLIERAVTSLTARGVTFLTGLPV--TNVAGNV 238 +YGV+ ++K+ VEA PK+LP+ + L+ AV+ L RGV F P+ N G V Sbjct: 185 CSKYGVDQNKVKITCVEAAPKMLPMFSEELVNHAVSYLEDRGVEFKIATPIVACNEKGFV 244 Query: 239 IDLKDG--QQIVANTFVWTGGVQGNPLVGESGLEVNRGRATVNDFLQSTSHPDVFVAGD- 295 +++ DG QQ+ A T VW GV+G+ L+ ES V RGR L + ++FV GD Sbjct: 245 VEV-DGEKQQLNAGTSVWAAGVRGSKLMEESFEGVKRGRIVTKQDLTINGYDNIFVIGDC 303 Query: 296 SAVVFAADGRPYPPTAQIAWQMGELIGYNLYAYLTNAASEAFSPINSGTLASLGRKDGVA 355 SA + A + RP P TAQIA Q GE + N+ L ++E F ++ GT+ SLG DGV Sbjct: 304 SAFIPAGEERPLPTTAQIAMQQGESVAKNIKRILNGESTEEFEYVDRGTVCSLGSHDGVG 363 Query: 356 IIGASNTPLKGLPATLMKEASNIRYLSHIKGL 387 ++ P+ G A MK+ + R + I G+ Sbjct: 364 MVFGK--PIAGKKAAFMKKVIDTRAVFKIGGI 393 >BH3407 [C] COG1252 NADH dehydrogenase, FAD-containing subunit Length = 400 Score = 213 bits (541), Expect = 5e-55, Method: Compositional matrix adjust. Identities = 134/388 (34%), Positives = 200/388 (51%), Gaps = 9/388 (2%) Query: 4 HIVILGAGYGGLLSAINVRKYMSKAEAKITVVNQYPTHQIITELHRLAAGSVAEQAVAMP 63 +IVILGAGYGG+++A + K + +A IT+VN++ H T LH AAG++ + +P Sbjct: 5 NIVILGAGYGGIITASRLSKQLGHNDANITLVNKHSYHYQTTWLHEPAAGTIPAEKARVP 64 Query: 64 LTKLFAGKDIDLKIAKVKSFSVDSKQVQLSDGTTLSYDALVVALGSTTAYFGIPGLEEYS 123 + + ++ D+K+V L +G L YD LVVALGS FG+PG+ E++ Sbjct: 65 IKDVLDLNKVNFVQDTAVEIDRDAKKVVLENGE-LEYDYLVVALGSVAETFGVPGVFEHA 123 Query: 124 MVLKSAADAKQIHGHIEGRIREYAKTHNPADA--AXXXXXXXXXXXXXXXXXADVLPKLA 181 + A+Q+ HIE Y D + +P+L Sbjct: 124 FSKWTVNGARQVKEHIEYMFATYNNLEEKKDELLTFIVAGAGFTGIEFVGELSKRVPELC 183 Query: 182 KRYGVNPQEIKLMLVEAGPKILPVLPDHLIERAVTSLTARGVTFLTGLPVTNVAGNVIDL 241 Y V +++K+ ++EA P LP L+E A+ L +RGV F P+ V + + L Sbjct: 184 ATYDVPREKVKMYVIEAAPTALPGFDPELVEYAMNLLESRGVEFKINCPIKEVVKDGVIL 243 Query: 242 KDGQQIVANTFVWTGGVQGNPLVGESGLEVNRGRATVNDFLQSTSHPDVFVAGDSAVVFA 301 +G +I +NT VW GV+G ++ +SG E RGR V L++ H ++FV GD A++ Sbjct: 244 ANGDEIKSNTVVWATGVRGPEIIEKSGFEAMRGRIKVESDLRAPGHEEIFVIGDCALIIN 303 Query: 302 AD-GRPYPPTAQIAWQMGELIGYNLYAYLTNAASE--AFSPINSGTLASLGRKDGVAIIG 358 + RPYPPTAQIA QM E +NL A L SE F P GT+ASLG K+ + ++G Sbjct: 304 EEINRPYPPTAQIAIQMAETCAHNL-ATLVKGGSELKNFKPEIKGTVASLGGKEAIGVVG 362 Query: 359 ASNTPLKGLPATLMKEASNIRYLSHIKG 386 L G A+ MK+ + R L I G Sbjct: 363 TRK--LFGSSASFMKKVIDNRSLFLIGG 388 >BS_yumB [C] COG1252 NADH dehydrogenase, FAD-containing subunit Length = 406 Score = 211 bits (536), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 135/390 (34%), Positives = 198/390 (50%), Gaps = 10/390 (2%) Query: 5 IVILGAGYGGLLSAINVRKYMSKAEAKITVVNQYPTHQIITELHRLAAGSVAEQAVAMPL 64 IVILGAGYGGL++ + KY+ +A IT+VN++ H T +H +AG++ + Sbjct: 8 IVILGAGYGGLMTVTRLTKYVGPNDADITLVNKHNYHYETTWMHEASAGTLHHDRCRYQI 67 Query: 65 TKLFAGKDIDLKIAKVKSFSVDSKQVQLSDGTTLSYDALVVALGSTTAYFGIPGLEEYSM 124 + ++ VK+ +D K+V L++G L YD LV+ LG+ FGI GL+EY+ Sbjct: 68 KDVINQSRVNFVQDTVKAIKIDEKKVVLANGE-LQYDYLVIGLGAVPETFGIKGLKEYAF 126 Query: 125 VLKSAADAKQIHGHIEGRIREY--AKTHNPADAAXXXXXXXXXXXXXXXXXADVLPKLAK 182 + + ++ + HIE + Y P A +P+L K Sbjct: 127 PIANINTSRLLREHIELQFATYNTEAEKRPDRLTIVVGGAGFTGIEFLGELAARVPELCK 186 Query: 183 RYGVNPQEIKLMLVEAGPKILPVLPDHLIERAVTSLTARGVTFLTGLPVTNVA--GNVID 240 Y V+ ++++ VEA P +LP L++ AV L GV F G V G + Sbjct: 187 EYDVDRSLVRIICVEAAPTVLPGFDPELVDYAVHYLEENGVEFKIGTAVQECTPEGVRVG 246 Query: 241 LKDGQ--QIVANTFVWTGGVQGNPLVGESGLEVNRGRATVNDFLQSTSHPDVFVAGDSAV 298 KD + QI + T VW GV+G+P+V E+G E RGR VN L++ H +VF+ GDS++ Sbjct: 247 KKDEEPEQIKSQTVVWAAGVRGHPIVEEAGFENMRGRVKVNPDLRAPGHDNVFILGDSSL 306 Query: 299 VFAADG-RPYPPTAQIAWQMGELIGYNLYAYLTNAASEAFSPINSGTLASLGRKDGVAII 357 D RPYPPTAQIA Q G + NL + E F P GT+ASLG + V ++ Sbjct: 307 FMNEDTERPYPPTAQIAMQQGITVAKNLGRLIKGGELEEFKPDIKGTVASLGEHNAVGVV 366 Query: 358 GASNTPLKGLPATLMKEASNIRYLSHIKGL 387 LKG PA+ MK+ + R L I GL Sbjct: 367 YGRK--LKGTPASFMKKVIDNRSLFMIGGL 394 >lin2488 [C] COG1252 NADH dehydrogenase, FAD-containing subunit Length = 403 Score = 196 bits (498), Expect = 5e-50, Method: Compositional matrix adjust. Identities = 129/399 (32%), Positives = 195/399 (48%), Gaps = 13/399 (3%) Query: 1 MSK-HIVILGAGYGGLLSAINVRKYMSKA-EAKITVVNQYPTHQIITELHRLAAGSVAEQ 58 MSK IVILGAGYGGL + +RK + +A+I +VN+ H T LH AAG++ + Sbjct: 1 MSKPKIVILGAGYGGLKT---LRKLQQRNLDAEIVLVNKNDYHHETTWLHEAAAGTIEPE 57 Query: 59 AVAMPLTKLFAGKDIDLKIAKVKSFSVDSKQVQLSDGTTLSYDALVVALGSTTAYFGIPG 118 + PL K+ V + D K V L +SYD L++ LGS FGI G Sbjct: 58 KLMYPLDKVVNNTKTTFIQDTVVKINKDEKTVTLDANGDISYDYLLIGLGSEAETFGISG 117 Query: 119 LEEYSMVLKSAADAKQIHGHIEGRIREYAKTHNPADAAXXXXXXXXXXXXXXXXXADVLP 178 L+EY+ + S K+I HIE + ++ + +P Sbjct: 118 LKEYAFTITSVESVKKIRAHIEAQFAKWKTDPRDELLTIIVGGAGFTGIEFLGELTNRIP 177 Query: 179 KLAKRYGVNPQEIKLMLVEAGPKILPVLPDHLIERAVTSLTARGVTFLTGLPVTNVAGNV 238 +L K Y V +++++ +EA PK+LP L++ V L RGV F G PV + Sbjct: 178 ELVKEYDVPREKVRIYCMEAAPKVLPQFDAKLVDYGVGVLEDRGVEFHVGKPVKEATADG 237 Query: 239 IDLKDGQ----QIVANTFVWTGGVQGNPLVGESGLEVNRGRATVNDFLQSTSHPDVFVAG 294 + + + +I A T +W GV+GN ++ SG E RGR VN+ L + ++ + G Sbjct: 238 VKYAESETEVREIKAATIIWAAGVRGNSVIEASGFEAGRGRVKVNNNLTVPGNEEILIVG 297 Query: 295 DSAVVF-AADGRPYPPTAQIAWQMGELIGYNLYAYLTNAAS-EAFSPINSGTLASLGRKD 352 D +++ A+ RP+PPTAQIA Q ++ NL + + F GT+ SLG D Sbjct: 298 DCSLIINPANDRPFPPTAQIAMQQADVAAVNLAKLVKGETDLQDFVYHEKGTVCSLGDND 357 Query: 353 GVAIIGASNTPLKGLPATLMKEASNIRYLSHIKGLFSLA 391 + ++ N LKG PA++MK+ + R L I G LA Sbjct: 358 AIGVVFGKN--LKGYPASVMKKVIDDRALLLIGGSGVLA 394 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.133 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,593,561 Number of extensions: 1116398 Number of successful extensions: 5467 Number of sequences better than 1.0e-05: 47 Number of HSP's gapped: 5389 Number of HSP's successfully gapped: 52 Length of query: 392 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 284 Effective length of database: 25,922,359 Effective search space: 7361949956 Effective search space used: 7361949956 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 117 (49.7 bits)