BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0574.0019.p (251 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yflG [J] COG0024 Methionine aminopeptidase 256 3e-68 AGc4195 [J] COG0024 Methionine aminopeptidase 242 3e-64 SMb21002 [J] COG0024 Methionine aminopeptidase 242 5e-64 BS_map [J] COG0024 Methionine aminopeptidase 165 7e-41 Rv2861c [J] COG0024 Methionine aminopeptidase 165 7e-41 >BS_yflG [J] COG0024 Methionine aminopeptidase Length = 249 Score = 256 bits (653), Expect = 3e-68, Method: Compositional matrix adjust. Identities = 120/241 (49%), Positives = 164/241 (68%), Gaps = 1/241 (0%) Query: 10 QDLQGLKAVGHVVGYTIAEMRKRTVPGMTTAELDEVGAAVLERFGAKSAPKVTYNFPGST 69 Q+L+GLK +G +V EM+++ PGM+T +LD +G AVL+ GA SAP+ Y+FPG T Sbjct: 7 QELEGLKKIGRIVALAREEMKRKAEPGMSTKDLDLIGKAVLDEHGAVSAPEKEYDFPGVT 66 Query: 70 CISINEEVAHGIPG-SRVIQAGDLVNIDVSAELNGYYGDAGASFQLPPYNEKLVHLCRSA 128 CIS+N+EVAHGIP S++++AGDLVNID+SAE G+Y D G SF L E+L LC+ A Sbjct: 67 CISVNDEVAHGIPSTSKILKAGDLVNIDISAEFGGFYSDTGISFVLGEGEERLHKLCQCA 126 Query: 129 EETMMSVINHLRAGMKVNEIGRVMETEARKRGYNVVRNLCSHGIGRSLHEKPFEILPFYN 188 E + +AG + N+IGR + EAR +G+ V++ L HGIGRSLHE P I+ +Y+ Sbjct: 127 ENAFQKGLQQAKAGKRQNQIGRAVYHEARSQGFTVIKTLTGHGIGRSLHEAPNHIMNYYD 186 Query: 189 PRVTTVLKEGQVITVEPFLSTGADFVEQQSDGWTLSVNDSSRVAQYEHTIIVTKGKPIIL 248 P + K G VI +EPF+ST A+ + + DGWT D S VAQ EHTI++TK +PIIL Sbjct: 187 PFDNALFKNGTVIALEPFISTKAETIVEAGDGWTFKTPDKSMVAQVEHTIVITKDEPIIL 246 Query: 249 T 249 T Sbjct: 247 T 247 >AGc4195 [J] COG0024 Methionine aminopeptidase Length = 250 Score = 242 bits (618), Expect = 3e-64, Method: Compositional matrix adjust. Identities = 117/247 (47%), Positives = 160/247 (64%), Gaps = 1/247 (0%) Query: 4 MTSDTAQDLQGLKAVGHVVGYTIAEMRKRTVPGMTTAELDEVGAAVLERFGAKSAPKVTY 63 M T ++L LK +G + + M PG+TTAELD +G VLE GA+SAP+ Y Sbjct: 1 MVISTDEELDLLKDIGRLCAVAMQTMADALEPGITTAELDAIGRKVLEDNGAQSAPEFCY 60 Query: 64 NFPGSTCISINEEVAHGIPGSRVIQAGDLVNIDVSAELNGYYGDAGASFQLPPYNEKLVH 123 FPG+TCIS+NEEVAHGIPG R+I+AGDLVNIDVSA NG++GD G+SF +PP + + Sbjct: 61 KFPGATCISVNEEVAHGIPGPRIIKAGDLVNIDVSAVKNGFFGDTGSSFAVPPVKKDIER 120 Query: 124 LCRSAEETMMSVINHLRAGMKVNEIGRVMETEARKRGYNVVRNLCSHGIGRSLHEKPFEI 183 LCR + M + + ++ G +IG + A+K Y ++ NL SHGIGRSLHE+P E+ Sbjct: 121 LCRDGKRAMWTGLQQVKTGRPFADIGNAIGAFAKKNRYTLITNLASHGIGRSLHEEPKEL 180 Query: 184 LPFYNPRVTTVLKEGQVITVEPFLSTGADFVEQ-QSDGWTLSVNDSSRVAQYEHTIIVTK 242 + + +++EG V TVEPFLSTGA + E D WTL + S+ QYEHT++ TK Sbjct: 181 ATWPDKDERRIMQEGMVFTVEPFLSTGAYWAEDGDDDPWTLYSDPSAPTVQYEHTVVATK 240 Query: 243 GKPIILT 249 P++LT Sbjct: 241 NGPLVLT 247 >SMb21002 [J] COG0024 Methionine aminopeptidase Length = 249 Score = 242 bits (617), Expect = 5e-64, Method: Compositional matrix adjust. Identities = 118/246 (47%), Positives = 158/246 (64%) Query: 4 MTSDTAQDLQGLKAVGHVVGYTIAEMRKRTVPGMTTAELDEVGAAVLERFGAKSAPKVTY 63 MT + +DL+ LK +G + + M + G+TTAELD +G VLE GA+SAP++ Y Sbjct: 1 MTLNNDEDLERLKEIGRICANALQAMGEALELGITTAELDAIGRKVLEDAGARSAPELCY 60 Query: 64 NFPGSTCISINEEVAHGIPGSRVIQAGDLVNIDVSAELNGYYGDAGASFQLPPYNEKLVH 123 +FPG+TCIS+NEE+AHGIPG RVIQ GDLVNIDVSAE G + D GASF +PP ++ Sbjct: 61 DFPGATCISVNEEIAHGIPGRRVIQPGDLVNIDVSAEKGGLFADTGASFPVPPVKTEIDR 120 Query: 124 LCRSAEETMMSVINHLRAGMKVNEIGRVMETEARKRGYNVVRNLCSHGIGRSLHEKPFEI 183 LCR + M + ++ G + IG + ARK Y++V NL SHGIGRSLHE+P EI Sbjct: 121 LCRDGKRAMWVGLKQVKPGQSLAAIGNSIGEFARKNRYSLVANLASHGIGRSLHEEPSEI 180 Query: 184 LPFYNPRVTTVLKEGQVITVEPFLSTGADFVEQQSDGWTLSVNDSSRVAQYEHTIIVTKG 243 + +P + EG V TVEPFLS GA + E D WTL + QYEHT++VT+ Sbjct: 181 ATWPDPSERRRMTEGMVFTVEPFLSMGAQWAEGGDDDWTLYSEPRAPTVQYEHTVVVTRS 240 Query: 244 KPIILT 249 P+++T Sbjct: 241 GPLVVT 246 >BS_map [J] COG0024 Methionine aminopeptidase Length = 248 Score = 165 bits (417), Expect = 7e-41, Method: Compositional matrix adjust. Identities = 83/243 (34%), Positives = 142/243 (58%), Gaps = 3/243 (1%) Query: 8 TAQDLQGLKAVGHVVGYTIAEMRKRTVPGMTTAELDEVGAAVLERFGAKSAPKVTYNFPG 67 T ++L ++ G +V T E++K PG++T ELD++ +++ GA + K F G Sbjct: 6 TPRELGIMREAGRIVALTHEELKKHIKPGISTKELDQIAERFIKKQGAIPSFKGYNGFRG 65 Query: 68 STCISINEEVAHGIPGSRVIQAGDLVNIDVSAELNGYYGDAGASFQLPPYNEKLVHLCRS 127 S C+S+NEE+ HGIPGSRV++ GD+++ID+ A+LNGY+GD+ ++ + ++ L Sbjct: 66 SICVSVNEELVHGIPGSRVLKDGDIISIDIGAKLNGYHGDSAWTYPVGNISDDDKKLLEV 125 Query: 128 AEETMMSVINHLRAGMKVNEIGRVMETEARKRGYNVVRNLCSHGIGRSLHEKPFEILPFY 187 EE++ + + G +++ I ++T ++VVR HG+G+ LHE P +P Y Sbjct: 126 TEESLYKGLQEAKPGERLSNISHAIQTYVENEQFSVVREYVGHGVGQDLHEDPQ--IPHY 183 Query: 188 N-PRVTTVLKEGQVITVEPFLSTGADFVEQQSDGWTLSVNDSSRVAQYEHTIIVTKGKPI 246 P LK G V+ +EP ++ G+ +V+ +D WT+ D + A +EHTI +T+ Sbjct: 184 GPPNKGPRLKPGMVLAIEPMVNAGSRYVKTLADNWTVVTVDGKKCAHFEHTIAITETGFD 243 Query: 247 ILT 249 ILT Sbjct: 244 ILT 246 >Rv2861c [J] COG0024 Methionine aminopeptidase Length = 285 Score = 165 bits (417), Expect = 7e-41, Method: Compositional matrix adjust. Identities = 91/242 (37%), Positives = 131/242 (54%), Gaps = 1/242 (0%) Query: 8 TAQDLQGLKAVGHVVGYTIAEMRKRTVPGMTTAELDEVGAAVLERFGAKSAPKVTYNFPG 67 T + ++ ++ G + +AE K PG+TT ELD + L GA + FP Sbjct: 43 TPEVIEKMRVAGRIAAGALAEAGKAVAPGVTTDELDRIAHEYLVDNGAYPSTLGYKGFPK 102 Query: 68 STCISINEEVAHGIPGSRVIQAGDLVNIDVSAELNGYYGDAGASFQLPPYNEKLVHLCRS 127 S C S+NE + HGIP S VI GD+VNIDV+A + G +GD A+F ++ L Sbjct: 103 SCCTSLNEVICHGIPDSTVITDGDIVNIDVTAYIGGVHGDTNATFPAGDVADEHRLLVDR 162 Query: 128 AEETMMSVINHLRAGMKVNEIGRVMETEARKRGYNVVRNLCSHGIGRSLHEKPFEILPFY 187 E M IN ++ G ++ IGRV+E+ A + GYNVVR+ HGIG + H +L + Sbjct: 163 TREATMRAINTVKPGRALSVIGRVIESYANRFGYNVVRDFTGHGIGTTFHNG-LVVLHYD 221 Query: 188 NPRVTTVLKEGQVITVEPFLSTGADFVEQQSDGWTLSVNDSSRVAQYEHTIIVTKGKPII 247 P V T+++ G T+EP ++ GA E DGWT+ D AQ+EHT++VT I Sbjct: 222 QPAVETIMQPGMTFTIEPMINLGALDYEIWDDGWTVVTKDRKWTAQFEHTLLVTDTGVEI 281 Query: 248 LT 249 LT Sbjct: 282 LT 283 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.133 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,789,085 Number of extensions: 678814 Number of successful extensions: 2213 Number of sequences better than 1.0e-05: 50 Number of HSP's gapped: 2156 Number of HSP's successfully gapped: 50 Length of query: 251 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 147 Effective length of database: 26,383,695 Effective search space: 3878403165 Effective search space used: 3878403165 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 114 (48.5 bits)