BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0574.0015.p (140 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ywnA [K] COG1959 Predicted transcriptional regulator 135 2e-32 BH0306 [K] COG1959 Predicted transcriptional regulator 129 1e-30 RSp0526 [K] COG1959 Predicted transcriptional regulator 102 2e-22 lin0458 [K] COG1959 Predicted transcriptional regulator 89 1e-18 AGc4027 [E] COG0006 Xaa-Pro aminopeptidase 70 1e-12 >BS_ywnA [K] COG1959 Predicted transcriptional regulator Length = 133 Score = 135 bits (340), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 67/135 (49%), Positives = 92/135 (68%), Gaps = 4/135 (2%) Query: 3 ISTRFAVAIHILTLIDSNKEGKSTSEWIAGSVNTNPVVIRRLTGMLHKAGLVEVRPGVAG 62 I++R AVAIHIL+LI ++ K++SE IA SVNTNPVV+RR+ +L KA ++ R GV G Sbjct: 2 INSRLAVAIHILSLISMDE--KTSSEIIADSVNTNPVVVRRMISLLKKADILTSRAGVPG 59 Query: 63 TKLARSPGEITLLEIYRAVNAAEADTLFSVHEHPNPNCPVGKNIAGAIVPVFSLAQQALE 122 L + P +I+LLE+YRAV E LF+VHE+PNP CPVGK I A+ F Q+A+E Sbjct: 60 ASLKKDPADISLLEVYRAVQKQE--ELFAVHENPNPKCPVGKKIQNALDETFESVQRAME 117 Query: 123 NVLQGVTLDQIVNQI 137 N L +L ++N + Sbjct: 118 NELASKSLKDVMNHL 132 >BH0306 [K] COG1959 Predicted transcriptional regulator Length = 143 Score = 129 bits (325), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 65/135 (48%), Positives = 90/135 (66%), Gaps = 2/135 (1%) Query: 2 NISTRFAVAIHILTLIDSNKEGKSTSEWIAGSVNTNPVVIRRLTGMLHKAGLVEVRPGVA 61 IS+RF++A+HILT I S +EG T ++IAGSVN+NPV++R + GML KA L+ VRPGV Sbjct: 3 QISSRFSIAVHILTFIASVREG-CTGDFIAGSVNSNPVIVRNIMGMLKKADLIGVRPGVG 61 Query: 62 GTKLARSPGEITLLEIYRAVNAAEADTLFSVHEHPNPNCPVGKNIAGAIVPVFSLAQQAL 121 G L + EITLL++YRAV+ D LF H +P C VG+ I + AQ+A+ Sbjct: 62 GAYLKKDADEITLLDVYRAVDVIGDDQLFGFH-NPKIECEVGRIIENTLREELKQAQEAM 120 Query: 122 ENVLQGVTLDQIVNQ 136 E LQ VT+ Q+++Q Sbjct: 121 EQRLQLVTIQQLLDQ 135 >RSp0526 [K] COG1959 Predicted transcriptional regulator Length = 143 Score = 102 bits (254), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 61/139 (43%), Positives = 86/139 (61%), Gaps = 6/139 (4%) Query: 1 MNISTRFAVAIHILTLIDSNKEGKSTSEWIAGSVNTNPVVIRRLTGMLHKAGLVEVRPGV 60 M+ S+RFAVA+HILTL+ S E S IAGSV TNP +IRRL +L +AG V + G Sbjct: 1 MSTSSRFAVAVHILTLLASAGE-PVPSSLIAGSVGTNPALIRRLVALLAEAGFVTSQMGA 59 Query: 61 A-GTKLARSPGEITLLEIYRAVNAAEADTLFSV-HEHPNPNCPVGKNIAGAIVPVFSLAQ 118 + G+ LAR ITLL+++RAV E+ L ++ H PNP C VG+ I G + V + AQ Sbjct: 60 SGGSTLARPADRITLLDVFRAV---ESPVLIALPHSQPNPACEVGREITGVLERVTARAQ 116 Query: 119 QALENVLQGVTLDQIVNQI 137 A+E L T+ ++ ++ Sbjct: 117 SAMEAELAAQTIAGMLTEV 135 >lin0458 [K] COG1959 Predicted transcriptional regulator Length = 155 Score = 89.4 bits (220), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 56/141 (39%), Positives = 78/141 (55%), Gaps = 5/141 (3%) Query: 1 MNISTRFAVAIHILTLIDSNKEGKS-TSEWIAGSVNTNPVVIRRLTGMLHKAGLVEVRPG 59 M S +F+ AIHIL I+ K +SE IA SV TNP IRR+ L KA L+ + G Sbjct: 1 MKYSIQFSDAIHILAYIEIFKNTNLLSSEIIASSVKTNPANIRRIMSNLKKANLITTQTG 60 Query: 60 VAGTKLARSPGEITLLEIYRAVNAAEADT-LFSVHEHPNPNCPVGKNIAGAIVPVFSLAQ 118 A L + P EI+LL++Y+++ E +T L V NP+C VG NI + + L Q Sbjct: 61 KANPILTKPPEEISLLDVYKSI---EGNTNLIHVDPKTNPDCIVGANIQQVLSSKYELLQ 117 Query: 119 QALENVLQGVTLDQIVNQIPV 139 Q +E + + LD IV+ I V Sbjct: 118 QKIELEMDQIKLDSIVHDISV 138 >AGc4027 [E] COG0006 Xaa-Pro aminopeptidase Length = 152 Score = 69.7 bits (169), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 50/138 (36%), Positives = 73/138 (52%), Gaps = 6/138 (4%) Query: 1 MNISTRFAVAIHILTLIDSNKEGKSTSEWIAGSVNTNPVVIRRLTGMLHKAGLVEVRPGV 60 M TR + +HIL ++ + EG +TSE IA + TNPVV+RR L + G V G Sbjct: 1 MRHDTRLSRVLHILIHMEKH-EGAATSENIAAMLQTNPVVVRRTMAGLREHGYVSSEKGH 59 Query: 61 AGTK-LARSPGEITLLEIYRAVNAAEADTLFSVH-EHPNPNCPVGKNIAGAIVPVFSLAQ 118 G LA+ EITLL+IYRA+ A E LFS+ NPNC + + + A+ + A+ Sbjct: 60 GGGWVLAQPLTEITLLDIYRALGAPE---LFSIGLAGDNPNCVIEQAVNAALFDAMNEAE 116 Query: 119 QALENVLQGVTLDQIVNQ 136 L + +TL + + Sbjct: 117 NILLSRFGSITLSTLAEE 134 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.133 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 10,161,877 Number of extensions: 355494 Number of successful extensions: 659 Number of sequences better than 1.0e-05: 8 Number of HSP's gapped: 651 Number of HSP's successfully gapped: 8 Length of query: 140 Length of database: 38,378,431 Length adjustment: 96 Effective length of query: 44 Effective length of database: 27,306,367 Effective search space: 1201480148 Effective search space used: 1201480148 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 110 (47.0 bits)