BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0574.0007.p (251 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC1386 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 202 6e-52 BS_yflN [R] COG0491 Zn-dependent hydrolases, including glyoxylases 76 5e-14 BH1837 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 74 2e-13 VNG0231C [R] COG0491 Zn-dependent hydrolases, including glyoxylases 64 2e-10 DR2076 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 53 4e-07 >CAC1386 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 243 Score = 202 bits (513), Expect = 6e-52, Method: Compositional matrix adjust. Identities = 109/248 (43%), Positives = 143/248 (57%), Gaps = 5/248 (2%) Query: 1 MHIAKGIEMLEISVPVMGGTDTFYPTLIWDEEEAVLIDTGVPGSAQKFKEATAAASVPWN 60 M I G+EMLE+ VMG TL++DEE VL+DTG PG + +EA A V + Sbjct: 1 MKIVDGVEMLELESNVMGKKSVINVTLLYDEENVVLVDTGFPGMSAYIREAVEKAGVALD 60 Query: 61 LVRTILITHQDIDHIGSLPALLEQSESKITVLAHEIERPYIEGKQMLLKHTXXXXXXXXX 120 V I++THQDIDHIGSL +++++S KI V +HE E+PYIEG + LK Sbjct: 61 KVNKIILTHQDIDHIGSLASIIKESNKKIEVFSHEEEKPYIEGTKTPLKLVKFQDKANTS 120 Query: 121 XXXKNISEEWKRAFLHVLAHPPRAGVDRTLTGGEVLPIGGGIAVIHTPGHSPGHLSLYHQ 180 + I E+ K F +A P V+ TL GE LP GGI VI TPGH+ GH+ LY + Sbjct: 121 DQIRAICEKLKIGF--EMAKSP---VNTTLKDGEKLPFCGGIEVIQTPGHTFGHICLYIE 175 Query: 181 ASRTLIAADALTVRGGELCGPDAAATPDMAEALQSLHKFAAFEINQVICYHGGLFRGDVN 240 S+ LIA DAL + G L D D + +SL K ++I VICYHGGL +G+VN Sbjct: 176 KSKLLIAGDALGAQDGVLMLADPLFDFDSNLSKKSLEKLTKYDIETVICYHGGLVKGNVN 235 Query: 241 RRIAELAQ 248 RI EL + Sbjct: 236 ERIKELIK 243 >BS_yflN [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 264 Score = 75.9 bits (185), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 77/271 (28%), Positives = 116/271 (42%), Gaps = 57/271 (21%) Query: 4 AKGIEMLEISVPVMGGTDTFYPTLIWDEEEAVLIDTGVPGSA--------QKFKEATAAA 55 AKG+ L + + + ++ L + VLID G+P SA Q+F E Sbjct: 20 AKGVHGLTVQI-----ANVYFIQLPSEPHSFVLIDAGMPQSAGVIVNEAKQRFGEGFQ-- 72 Query: 56 SVPWNLVRTILITHQDIDHIGSLPALLEQSESKITVLAHEIERPYIEGKQMLLKHTXXXX 115 ++ I++TH DHIG++ +LE + + V H E PY+ GK+ Sbjct: 73 ------LKAIILTHGHFDHIGAIEEILEHWD--VPVYIHSREMPYVTGKE---------- 114 Query: 116 XXXXXXXXKNISEEWKRAFLHVLAHPPRAGVDRTLTGGEVLPIGGGIAV------IHTPG 169 + S+ A L L PR +D + + + LP G + I TPG Sbjct: 115 ---DYPPARPDSKSGLVAKLSPLF--PRHSIDIS-SHVQALPEDGSVPFLDEWMWIATPG 168 Query: 170 HSPGHLSLYHQASRTLIAADALT-----------VRGGELCGPDAAATPDMAEALQSLHK 218 H+PGH+SL+ R L+A DA+ ++ EL GP A TPD A +S+ K Sbjct: 169 HTPGHISLFRDDGRVLVAGDAVITVEQEKMADVLIQKQELHGPPAYFTPDTETAAESILK 228 Query: 219 FAAFEINQVICYHGGLFRGDVNRR-IAELAQ 248 A E ++ HG G R + ELA Sbjct: 229 LAGLEPEALLTGHGIPMTGKNFRSDLTELAN 259 >BH1837 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 236 Score = 73.9 bits (180), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 58/210 (27%), Positives = 92/210 (43%), Gaps = 35/210 (16%) Query: 27 LIWDEEEAVLIDTGVPGSAQK-FKEATAAASVPWNLVRTILITHQDIDHIGSLPALLEQS 85 ++ ++E ++DTG+P ++ KEA V + +L+TH DH+G L +L+ Sbjct: 25 IVKEDEGVYIVDTGMPYMGKRILKEAEKLGKV-----KGVLLTHGHSDHVGGLKKILDIQ 79 Query: 86 ESKITVLAHEIERPYIEGKQMLLKHTXXXXXXXXXXXXKNISEEWKRAFLHVLAHPPRAG 145 + + V H ++ ++EGK+ KR +L G Sbjct: 80 D--LPVYVHTLDIKHMEGKEPF-------------------PGRKKREYL------VEPG 112 Query: 146 VDRTLTGGE--VLPIGGGIAVIHTPGHSPGHLSLYHQASRTLIAADALTVRGGELCGPDA 203 V + L E G + HTPGHSPGH+ YH+ R LI D T + G+L P Sbjct: 113 VAKPLPVDEDGEFRCFGNLVPYHTPGHSPGHVCYYHKKDRVLIGGDLFTSKRGKLQQPIK 172 Query: 204 AATPDMAEALQSLHKFAAFEINQVICYHGG 233 T DM EA++S + ++V HG Sbjct: 173 MFTADMNEAVRSGEIVKKLQPSKVSICHGN 202 >VNG0231C [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 236 Score = 63.9 bits (154), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 65/229 (28%), Positives = 98/229 (42%), Gaps = 38/229 (16%) Query: 27 LIWDEEEAVLIDTGVPGSAQKFKEATAAASVPWNLVRTILITHQDIDHIGSLPALLEQSE 86 ++ D+ E L+D G PG Q ++ A V + +L+TH D DH+G+L L + Sbjct: 33 VVVDDNETTLVDAGWPGDEQTVRDGLHTAGVEPGDIDRVLVTHYDADHVGTLARLTPALD 92 Query: 87 SKITVLAHEIERPYIEGKQMLLKHTXXXXXXXXXXXXKNISEEWKRAFLHVLAHPP---R 143 + + V HE + PY+ G ++ +N R + L P R Sbjct: 93 APVYV--HERDAPYVAGDRL------------PPWTARNGIGALHRLYYRRLDLPDLPIR 138 Query: 144 AGVDRTLTGGEVLPIGGGIAVIHTPGHSPGHLSLYHQASRTLIAADALTVRG------GE 197 A VD G ++ GG HTPGH+PGH++ H+ D G G Sbjct: 139 AVVD----GDDI----GGFHAYHTPGHTPGHVAYVHEDLSVAFLGDLAYGLGDRLKPTGW 190 Query: 198 LCGPDAAATPDMAEALQSLHKFA-AFEINQVICYHGGLFRGDVNRRIAE 245 L DAAA + +++SL + A AFE C G D + R+AE Sbjct: 191 LTSSDAAA---VESSVRSLCRRAPAFEY---ACPGHGPPLPDGDDRLAE 233 >DR2076 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 347 Score = 53.1 bits (126), Expect = 4e-07, Method: Compositional matrix adjust. Identities = 58/219 (26%), Positives = 88/219 (40%), Gaps = 14/219 (6%) Query: 35 VLIDTGV--PGSAQKFKEATAAASVPWNLVRTILITHQDIDHIGSLPALLEQSESKITVL 92 LID + P + ++ A W+ + +++TH DH G + E+S +++ +L Sbjct: 62 TLIDCALDTPEARAAIEDGLAQLGRHWSDIDRLIVTHHHPDHYGLAGVIEERSGARVQML 121 Query: 93 AHEI---ERPYIEGKQMLLKHTXXXXXXXX-XXXXKNISEEWKRAFLHVLAHPPRAGVDR 148 EI ER + + L H + I E R P R G Sbjct: 122 DVEIGRGERYWHMLSEWLPGHRKHMRDHGLPEASVEGIRAENPRVHPASRVEPLREGQSV 181 Query: 149 TLTGGEVLPIGGGIAVIHTPGHSPGHLSLYHQASRTLIAADALTVRGGELCGPDAAATPD 208 +L G E V+ PGH+ GHL L+++ LIA DA+ R G A PD Sbjct: 182 SLAGHE-------WEVLWLPGHADGHLGLWNEEESLLIAGDAILPRISPNIGLYAYTRPD 234 Query: 209 -MAEALQSLHKFAAFEINQVICYHGGLFRGDVNRRIAEL 246 + + LQ+L K A + + H G V R EL Sbjct: 235 PLGDYLQTLGKLEALNPRRAVVGHHGPVMEGVQARAREL 273 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,533,178 Number of extensions: 720710 Number of successful extensions: 1590 Number of sequences better than 1.0e-05: 15 Number of HSP's gapped: 1609 Number of HSP's successfully gapped: 15 Length of query: 251 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 147 Effective length of database: 26,383,695 Effective search space: 3878403165 Effective search space used: 3878403165 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 114 (48.5 bits)