BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0571.0030.p (182 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC0168 [S] COG1295 Predicted membrane protein 104 9e-23 CAC0485 [S] COG1295 Predicted membrane protein 96 2e-20 PA2751 [S] COG1295 Predicted membrane protein 80 1e-15 mll8068 [S] COG1295 Predicted membrane protein 59 3e-09 SMb21363 [S] COG1295 Predicted membrane protein 58 9e-09 >CAC0168 [S] COG1295 Predicted membrane protein Length = 273 Score = 104 bits (259), Expect = 9e-23, Method: Compositional matrix adjust. Identities = 55/174 (31%), Positives = 92/174 (52%) Query: 4 TLWAASKGINAIIKGLNRAYDIEESRVFWKIRGIAFLATXXXXXXXXXXXXXXXXXTWLK 63 T+W+AS G A+IKGLN+AY ++E+R F K+R ++ L T + Sbjct: 92 TIWSASAGFTAVIKGLNKAYGVQEARNFIKVRFVSILCTIGVAFIILTMMFLLILGRIIL 151 Query: 64 TQVFLLIDLPYGFQKIWDLLQYAVPLFVMFLVFTLLYWIAPSRRLTLKEVMPGAMFATLG 123 + + + +W+ ++Y + + +FT LY AP ++L EV+PG++F+TL Sbjct: 152 NYLAYKLGFSHKVIAVWEAVRYLIFVSTAIFIFTALYRYAPCKKLMWIEVLPGSLFSTLN 211 Query: 124 WIITSILFSVYVNQFSNFTETYGSLGGVMXXXXXXXXXXXXXXAGGEINAILMK 177 I+ S F+ YVN F+N++ YGS+G ++ GGEINA+L + Sbjct: 212 LILASFGFAYYVNNFANYSVIYGSIGAIIILLTWLFLVAVIVILGGEINAVLSQ 265 >CAC0485 [S] COG1295 Predicted membrane protein Length = 283 Score = 96.3 bits (238), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 55/179 (30%), Positives = 87/179 (48%), Gaps = 9/179 (5%) Query: 1 MLGTLWAASKGINAIIKGLNRAYDIEESRVFWKIRGIAFLATXXXXXXXXXXXXXXXXXT 60 ++ ++W+ G NA+I+GLN +Y E R F K++ I+ + T Sbjct: 96 LITSIWSCVSGFNAVIRGLNNSYKDNERRSFLKVQLISIIFTFGLIITIFFAMLLIVFGD 155 Query: 61 WLKTQVFLLIDLPYGFQK-----IWDLLQYAVPLFVMFLVFTLLYWIAPSRRLTLKEVMP 115 + LI +G + +WDL++Y + M F LY P+ + K V P Sbjct: 156 INEE----LIIKKFGINEFFGYGVWDLIKYGILTIGMIFSFAALYRYTPANKHKWKMVFP 211 Query: 116 GAMFATLGWIITSILFSVYVNQFSNFTETYGSLGGVMXXXXXXXXXXXXXXAGGEINAI 174 GA+ + LGW+I+SI F+ YVN F N+++ YGS+G V+ GGEINA+ Sbjct: 212 GAVISALGWVISSICFTYYVNNFGNYSKVYGSIGAVIVLMTWLFIISFIIICGGEINAV 270 >PA2751 [S] COG1295 Predicted membrane protein Length = 299 Score = 80.5 bits (197), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 44/176 (25%), Positives = 80/176 (45%) Query: 4 TLWAASKGINAIIKGLNRAYDIEESRVFWKIRGIAFLATXXXXXXXXXXXXXXXXXTWLK 63 LW +S GI +++ LN AYD++ESR WK ++ L T + Sbjct: 103 ALWTSSAGIRSMMIALNAAYDVKESRPVWKRFSLSILYTLGIALLLLVAAGLMVTGPQVI 162 Query: 64 TQVFLLIDLPYGFQKIWDLLQYAVPLFVMFLVFTLLYWIAPSRRLTLKEVMPGAMFATLG 123 + L+ L +W L++ + +F++ L L Y++ P + + PG++ + + Sbjct: 163 AWIAGLVGLEQYLVTLWTWLRWPLAVFLLMLAVALFYYLVPDVEQRFRFITPGSVLSVIA 222 Query: 124 WIITSILFSVYVNQFSNFTETYGSLGGVMXXXXXXXXXXXXXXAGGEINAILMKRS 179 WI S+ + YV F+N+ YGS+G ++ G E+NA++ S Sbjct: 223 WIAASLAYGFYVKNFANYNAMYGSIGAIIILLLYFYLSAAVVLFGAELNAVIEHHS 278 >mll8068 [S] COG1295 Predicted membrane protein Length = 353 Score = 59.3 bits (142), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 54/182 (29%), Positives = 78/182 (42%), Gaps = 15/182 (8%) Query: 4 TLWAASKGINAIIKGLNRAYDIEESRVFWKIRGIAFLATXXXXXXXXXXXXXXXXXTWLK 63 LW+++ G+ A+ +N AY+ E R F + ++ L T L Sbjct: 153 ALWSSNSGMKALFDAMNIAYEEREKRSFITLNLMSILFTVGALLIGIVL---------LL 203 Query: 64 TQVFLLIDLPYGFQKIWDLLQYAV---PLFV--MFLVFTLLYWIAPSR-RLTLKEVMPGA 117 T + L Y + W AV P+ V M +LLY PSR R + + GA Sbjct: 204 TVGVVPALLRYFYLDAWTETLVAVSRWPILVAAMLTGISLLYRFGPSRERAKWRWLSSGA 263 Query: 118 MFATLGWIITSILFSVYVNQFSNFTETYGSLGGVMXXXXXXXXXXXXXXAGGEINAILMK 177 + AT WI S LFS Y+ F+N+ TYGSLG V+ AG INA + Sbjct: 264 LIATAAWIAASWLFSFYLQHFANYNATYGSLGAVVGLMMWTWISVIILIAGAAINAEMEH 323 Query: 178 RS 179 ++ Sbjct: 324 QT 325 >SMb21363 [S] COG1295 Predicted membrane protein Length = 364 Score = 57.8 bits (138), Expect = 9e-09, Method: Compositional matrix adjust. Identities = 46/182 (25%), Positives = 84/182 (46%), Gaps = 7/182 (3%) Query: 1 MLGTLWAASKGINAIIKGLNRAYDIEESRVFWKIRGIAFLATXXXXXXXXXXXXXXXXXT 60 +L LW+A G+ A+ + +N AYD E R F ++ ++ T Sbjct: 163 LLFALWSARNGVGALFEAMNIAYDETEKRSFIRLTLLSIGFTLAGLILTAVLIAAIAVLP 222 Query: 61 WLKTQVFLLIDLPYGFQKIWDLLQYAVPLFVMFLVFTLLYWIAPSRR-LTLKEVMPGAMF 119 FL ++L + + +++ V L ++ TL+Y PSR L+ + GA Sbjct: 223 --AVLAFLHLEL----EGLLRFVRWPVMLLLIAAGITLIYRYGPSREPAKLRWLTWGAAL 276 Query: 120 ATLGWIITSILFSVYVNQFSNFTETYGSLGGVMXXXXXXXXXXXXXXAGGEINAILMKRS 179 +T+ W++ S+ FS Y++ F+N+ TYG+LG ++ G E+NA L ++ Sbjct: 277 STICWLVASLAFSYYIDNFANYNATYGALGALIGFMLWIWISTMIVIIGAELNAELEHQT 336 Query: 180 LK 181 + Sbjct: 337 AR 338 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.329 0.142 0.446 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 11,041,125 Number of extensions: 341231 Number of successful extensions: 963 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 965 Number of HSP's successfully gapped: 10 Length of query: 182 Length of database: 38,378,431 Length adjustment: 100 Effective length of query: 82 Effective length of database: 26,845,031 Effective search space: 2201292542 Effective search space used: 2201292542 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 112 (47.8 bits)