BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0571.0016.p (571 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1064 [G] COG1653 ABC-type sugar transport system, periplasmic ... 128 2e-29 BH0482 [G] COG1653 ABC-type sugar transport system, periplasmic ... 105 2e-22 BS_lplA [G] COG1653 ABC-type sugar transport system, periplasmic... 103 1e-21 SP1796 [G] COG1653 ABC-type sugar transport system, periplasmic ... 88 3e-17 YPO3472 [G] COG1653 ABC-type sugar transport system, periplasmic... 75 3e-13 >BH1064 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 550 Score = 128 bits (322), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 92/330 (27%), Positives = 156/330 (47%), Gaps = 34/330 (10%) Query: 2 VQRKGKHTVLILALVLMMITVAACGNNTNGKEQPTGNDSTAAVRPENDGPLAKYEPEIEL 61 ++++ H+ L+ L + ++T + +T E T D T + + Sbjct: 1 MKKRIVHSFLVGLLAVGILTACSQEGSTTEDETNTNEDGT-----------------VTI 43 Query: 62 TAMKNLYGDERYLPGDNLNNNVWTRAYEDELGIKVNYKWAVAG-EGFQQKVTMSIASND- 119 T + L + G+++++NV TR E+ELGIK +W E F ++ + ++S + Sbjct: 44 TTARTLDDGTVFKDGEDVHDNVVTRWAEEELGIKFVTEWTQPNDEQFNTQLRLMMSSGEK 103 Query: 120 LPDIMTL-PAKDYFTLAKAGKLADLTPYFDKYASDTLKSTLNADGGKQMKTAYV----DG 174 LPD+ + L +G + + + YAS LK +Q A+ DG Sbjct: 104 LPDVFQVNDTAIKADLLSSGLVMPVDEAIETYASPRLKQLF-----EQFPQAFYPSTKDG 158 Query: 175 KLYGIPQTGGSDATADMIWIRQDWLKNLNLSVPETLDDVIEIAKAFRNDDPDKNGVKDTF 234 YGIP+ G + + ++WIRQDWL +L P TL+++ I F N+DP++ G DT Sbjct: 159 VRYGIPRFSGGNGSDTLLWIRQDWLDKFDLEPPTTLEELEHIMDVFVNEDPNETGKNDTI 218 Query: 235 GIGFQK-DLTSSTGSFEGFFAAYHAYGK----AWIRGEDGKITYGAIKPGMKDALAKLRE 289 GI F D G+ + A+G AW +G+DG + YG+I+P MKD LAK+ E Sbjct: 219 GITFAAGDYGFRGGNIADSSWVFGAFGDFVPGAWSKGDDGSLVYGSIQPSMKDGLAKIHE 278 Query: 290 MFAEDLIDVEFGIKDWKKVGEDIAAGKIGL 319 + + + +F I E +G+ G+ Sbjct: 279 WYEKGYLATDFAILSAGDAQESFISGRSGI 308 Score = 70.9 bits (172), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 31/73 (42%), Positives = 49/73 (67%) Query: 496 RENNLFERSAWFGLPTKTMSQKGATLDKLQEETFIKILTGSAPVSDYDTFVENWKKLGGD 555 R+N+L + + G PT TM + L+++++E F+ I+ G+ P+ +D FVE W++ GG+ Sbjct: 476 RQNDLRIINEFNGPPTPTMVSRSEHLERMEDELFVDIIYGNKPLDAFDQFVEEWRQSGGE 535 Query: 556 QITEEVNEWYNST 568 ITEEVNEWY S Sbjct: 536 AITEEVNEWYESV 548 >BH0482 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 512 Score = 105 bits (263), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 119/465 (25%), Positives = 204/465 (43%), Gaps = 56/465 (12%) Query: 108 QQKVTMSIASNDLPDIMTLPAKDYFTLA-----KAGKLADLTPYFDKYASDTLKSTLNAD 162 + +V ++AS +LP ++TL +D+ T + ++G+ ++ PY D++ + L+ Sbjct: 93 EDRVNAALASGNLPKVITL--QDFRTSSVLNAFRSGQFWEIGPYLDEFPN------LSEM 144 Query: 163 GGKQMKTAYVDGKLYGIPQTGGSDATADMIWIRQDWLKNLNLSVPETLDDVIEIAKAFRN 222 ++ VDGK+YGI + + + IRQDWL+NL L +P L+++ E+A+AF Sbjct: 145 NETILENVSVDGKIYGIYRE--RPLSRQGVVIRQDWLENLGLEMPTNLEELYEVARAFTY 202 Query: 223 DDPDKNGVKDTFGIGFQKDLTSSTGSFEGFFAAYHAYGKAWIRGEDGKITYGAIKPGMKD 282 DDP+ +G DT G + DL G+F+ A+YH W ++GK+ + + Sbjct: 203 DDPNGSGADDTVGFTDRNDLI--FGAFKT-LASYHGTPNEWGFDDEGKLMPEFMTDEYVE 259 Query: 283 ALAKLREMFAEDLIDVEFGIKDWKKVGEDIAAGKIGLFYGREGLPWMSAKDAIVNNPKAE 342 + ++ ++ E LI+ EF + ++ E G+ G++ G A NP+ E Sbjct: 260 TMNYMKSLYDEGLINTEFPVTSKEQQQELFMTGRAGIYVGNMVDARNLRDKAKDINPEME 319 Query: 343 W-ISVPIVSSDGKPAKPATNVTMERYFAVRADVQYPEALIKIINLFQDKINNPKTTMEML 401 I+ I DG+ A FA+ EA +K I F D+I M Sbjct: 320 VAITNRIAGPDGEDRVWAQG-GHNGIFAIPKSSVETEAELKRILAFFDRIAEEDVYNLM- 377 Query: 402 NKFGVDPASGINFAAYAFGGLDPSEDKPNMYYKQINDVLEG-KEQLDNIHPEAVRYYNTI 460 A GI Y E Y+++I + L+ ++ + R N + Sbjct: 378 -------AYGIEDVHY--------ETIDGEYFERIEENLDDWAAEVQPLVSLVAREMNYL 422 Query: 461 KNYIDKGMDKGADPLGWNYYKFLGPESSWKIISYYRENNLFERSAWFGLPTKTMSQKGAT 520 K Y DPL Y + E + +II LF + T +++G Sbjct: 423 KAY--------GDPLYEEYERLT--EDNNEIIVTNPAEPLF---------SPTYTERGTE 463 Query: 521 LDKLQEETFIKILTGSAPVSDYDTFVENWKKLGGDQITEEVNEWY 565 L K+ ++ K + G ++ VE W GG+ I EE+NE Y Sbjct: 464 LQKIIDDATYKYILGELDEDGFEAEVERWLTNGGESIIEELNEDY 508 >BS_lplA [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 502 Score = 103 bits (256), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 75/241 (31%), Positives = 119/241 (49%), Gaps = 20/241 (8%) Query: 86 RAYEDELGIKVNYKWAVAGEGFQQKVTMSIASNDLPDIMTLPAKD--YFTLAKAGKLADL 143 +A E G ++ W V + K + +AS ++P + + K + KAG +L Sbjct: 58 KALEKYTGKEIKVTW-VPDSSYNDKFNIVMASGEMPHAIVIKDKSAGFIKSVKAGAFWEL 116 Query: 144 TPYFDKYASDTLKSTLNADGGKQMKTAYVDGKLYGIPQTGGSDATADMIWIRQDWLKNLN 203 +PY Y + L+ K +K + V+G++YGI +T D + IR DWLKN+ Sbjct: 117 SPYLKDYKN------LSQADEKILKNSSVNGEVYGIYRT--RDLIRACMIIRTDWLKNVG 168 Query: 204 LSVPETLDDVIEIAKAFRNDDPDKNGVKDTFGIGFQKDLTSSTGS----FEGFFAAYHAY 259 L +PETLDD E+ KAF+ DPD NG DT+G+ K + GS + +F A + Y Sbjct: 169 LDMPETLDDFYEVLKAFKEKDPDGNGKDDTYGMVVPKWMGLGNGSPWDVLQIWFGAPNRY 228 Query: 260 GKAWIRGEDGKITYGAIKPGMKDALAKLREMFAEDLIDVEFGIKDWKKVGEDIAAGKIGL 319 G E+GK+ DAL ++++ E LI+ +F + D K + + GK G+ Sbjct: 229 GV-----ENGKLIPDFTTKEYMDALTFFKKLYDEGLINKDFAVMDSAKWNDPVVKGKAGV 283 Query: 320 F 320 Sbjct: 284 I 284 >SP1796 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 538 Score = 88.2 bits (217), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 73/257 (28%), Positives = 114/257 (44%), Gaps = 23/257 (8%) Query: 80 NNNVWTRAYEDELGIKVNYKWAVAGEGFQQKVTMSIASNDLPDIMTLPAK---DYFTLAK 136 N + + E E G+ ++ W F +K + I+S DLPD + D AK Sbjct: 64 NEKLILQRLEKETGVHID--WTNYQSDFAEKRNLDISSGDLPDAIHNDGASDVDLMNWAK 121 Query: 137 AGKLADLTPYFDKYASDTLKSTLNADGGKQMKTAYVDGKLYGIP---QTGGS----DATA 189 G + + DKY + LK L+ + DG +Y P + G + Sbjct: 122 KGVIIPVEDLIDKYMPN-LKKILDEKPEYKALMTAPDGHIYSFPWIEELGDGKESIHSVN 180 Query: 190 DMIWIRQDWLKNLNLSVPETLDDVIEIAKAFRNDDPDKNGVKDTFGIGFQKDLTSSTGSF 249 DM WI +DWLK L L +P+T DD+I++ +AF+N DP+ NG D F S G+ Sbjct: 181 DMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNGNGEADEIPFSF----ISGNGN- 235 Query: 250 EGFFAAYHAYG-----KAWIRGEDGKITYGAIKPGMKDALAKLREMFAEDLIDVEFGIKD 304 E F + A+G + G DGK+ + A K+ + +R++ + LID E D Sbjct: 236 EDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVKFIRQLQEKGLIDKEAFEHD 295 Query: 305 WKKVGEDIAAGKIGLFY 321 W K G+++ Sbjct: 296 WNSYIAKGHDQKFGVYF 312 >YPO3472 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 437 Score = 75.1 bits (183), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 63/237 (26%), Positives = 110/237 (46%), Gaps = 32/237 (13%) Query: 71 ERYLPGDNLNNNVWTR---AYEDELGIKVNYKWAVAGEGFQQKVTMSIASNDLPDIMTLP 127 + ++ N + N++ + +E + GIK+ Y A F+Q++ + A N LPD++ Sbjct: 35 QVWIRASNDSKNIYKKEADTFEKKTGIKIEYFNATTD--FEQRLARAAAGNALPDLIF-- 90 Query: 128 AKDYFTLAKAGKLADLTPYFDKYASDTLKSTLNADGGKQM------KTAYVDGKLYGIPQ 181 D + + +L + P K N GG+ + T Y+DGK YG+P Sbjct: 91 -NDAVAIGQFVQLGIVEPIEPK----------NIIGGENIFDTAWKSTQYIDGKYYGVPT 139 Query: 182 TGGSDATADMIWIRQDWLKNLNLSVPETLDDVIEIAKAFRNDDPDKNGVKDTFGIGFQKD 241 + + A +++R+DW + L L P+ D+ +AKAF +DPD NG DT+G Sbjct: 140 SAQTFA----LFVRKDWREKLGLEQPKNWQDISTLAKAFTFNDPDGNGKNDTYGFILPGS 195 Query: 242 LTSSTGSFEGFFAAYH-AYGKAWIR-GEDGKITYGAIKPGMKDALAKLREMFAEDLI 296 T S+ F ++Y G +IR +GK +P + L+ +R M + + Sbjct: 196 TTRGYASW--FISSYLWQAGGDFIRPAGEGKFIASLEEPAATETLSFMRGMVCDKTV 250 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.135 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 47,449,666 Number of extensions: 2143946 Number of successful extensions: 4933 Number of sequences better than 1.0e-05: 6 Number of HSP's gapped: 4929 Number of HSP's successfully gapped: 7 Length of query: 571 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 459 Effective length of database: 25,461,023 Effective search space: 11686609557 Effective search space used: 11686609557 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 118 (50.1 bits)