BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0571.0004.p (373 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ykvI [S] COG3949 Uncharacterized membrane protein 221 2e-57 SA0609 [S] COG3949 Uncharacterized membrane protein 77 3e-14 BS_yyaD [S] COG3949 Uncharacterized membrane protein 72 1e-12 >BS_ykvI [S] COG3949 Uncharacterized membrane protein Length = 347 Score = 221 bits (562), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 122/333 (36%), Positives = 188/333 (56%), Gaps = 16/333 (4%) Query: 9 QIAFTYIGTIVGAGFATGQEILRFFTRYGHWAVLTILFSTVLFIWLGTKMMIIARRVQAE 68 Q+AF Y+GT+VGAGFATG+EI+ FF ++G + IL S +F LG K+MII++R+ A+ Sbjct: 10 QLAFVYVGTVVGAGFATGREIVEFFLKFGWFGFFGILVSGGMFTLLGAKLMIISKRINAK 69 Query: 69 SYEDFNRHLFGERAGRSISLFTMIILVGVNSIMLAGAGAIFQEHLGMPYQAGXXXXXXXX 128 SY++ N LFG AGR I++F + +L+GV S+ML+GAGA+F+E LGM Q G Sbjct: 70 SYQEMNIFLFGATAGRIINVFMLFVLLGVTSVMLSGAGALFEEQLGMSAQIGMLITIGLS 129 Query: 129 XXXXKRGISGILQINSLVVPLMLTLSLIIIFNTLGVPGAERFLFLPTDHSFFGAW----- 183 RG+ GI +N VVPL++ S+I++ A+ F+F + ++ W Sbjct: 130 LIVMTRGVKGIFGVNVFVVPLLIIFSMIVV--------ADSFIFSESRNAAQWVWPHRWD 181 Query: 184 --MSPLLYTAFNLGMAQAVLVPLARHTNDEQAXXXXXXXXXXXXXXXXXAAHFAMSSQMP 241 +S + Y A NL +AQAVLVPLA + E+ A+ ++S+ +P Sbjct: 182 WLLSAVSYGALNLSLAQAVLVPLANEMSSEKVIKKGALIGGTMLTIVLSASFLSLST-LP 240 Query: 242 GILQYEIPMGTIAIRLGPAVQSIYLLLIFMEIFSTFVADIYGVSIQLQQRXXXXXXXXXX 301 + Y+IPM + +V IYLL+IF E+F++ + ++YG+ Q+Q Sbjct: 241 DVFLYDIPMAQVVYLFARSVHLIYLLIIFGEVFTSVIGNLYGLEKQVQSFLPVKSKYIFA 300 Query: 302 XXXXICYVFSQFGFSSLLSIFYPIFGAVSLVWV 334 Y+ SQ G+ L+S YP+FG VSL ++ Sbjct: 301 AIMITAYITSQIGYGRLISTIYPLFGYVSLAFI 333 >SA0609 [S] COG3949 Uncharacterized membrane protein Length = 326 Score = 77.4 bits (189), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 66/309 (21%), Positives = 130/309 (42%), Gaps = 4/309 (1%) Query: 30 LRFFTRYGHWAVLTILFSTVLFIWLGTKMMIIARRVQAESYEDFNRHLFGERAGRSISLF 89 ++FFT+YG WA L ++ S + ++G ++ I +A ++E +++FGE+ + Sbjct: 1 MQFFTKYGLWAYLGVIISGFILAFIGRQVAKIGTAFEATNHESTLQYVFGEKFSKVFDYI 60 Query: 90 TMIILVGVNSIMLAGAGAIFQEHLGMPYQAGXXXXXXXXXXXXKRGISGILQINSLVVPL 149 + L G+ M+AGAGA F+E +P G + I++ +V P Sbjct: 61 LIFFLFGIAVTMIAGAGATFEESYNIPTWLGALIMTLAIYITLLLDFNKIVRALGIVTPF 120 Query: 150 MLTLSLII--IFNTLG-VPGAERFLFLPTDHSFFGAWMSPLLYTAFNLGMAQAVLVPLAR 206 ++ L ++I ++ G V AE +P + G W +Y + + +V + Sbjct: 121 LIVLVVLIAGVYLFKGHVSLAEVNQVVPEASIWKGIWFGT-IYGGLAFSVGFSTIVAIGG 179 Query: 207 HTNDEQAXXXXXXXXXXXXXXXXXAAHFAMSSQMPGILQYEIPMGTIAIRLGPAVQSIYL 266 T +FA+ S+ P I IP T+A + P + ++ Sbjct: 180 DTEKRTVSGAGAMYGGIIYTVLLALINFALQSEYPTIKNASIPTLTLANNIHPLIATVLS 239 Query: 267 LLIFMEIFSTFVADIYGVSIQLQQRXXXXXXXXXXXXXXICYVFSQFGFSSLLSIFYPIF 326 +++ +++T + +Y + + + Y+ S GF+ L++ Y I Sbjct: 240 VIMLAVMYNTILGLMYSFAARFTEPYSKNYHIFIIIMMVAGYLLSFVGFAELINKLYTIM 299 Query: 327 GAVSLVWVI 335 G V L V+ Sbjct: 300 GYVGLFIVV 308 >BS_yyaD [S] COG3949 Uncharacterized membrane protein Length = 338 Score = 72.0 bits (175), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 70/316 (22%), Positives = 136/316 (43%), Gaps = 14/316 (4%) Query: 15 IGTIVGAGFATGQEILRFFTRYGHWAVLTILFSTVLFIWLGTKMMIIARRVQAESYEDFN 74 +G+++GAG+A+GQEI +FF G + L I+ T++FI+ +M I+ +VQ+ + Sbjct: 14 LGSLIGAGYASGQEIWQFF---GAESGLAIVLFTIMFIFSSYIVMKISFKVQSTHFLPVL 70 Query: 75 RHLFGERAGRSISLFTMIILVGVNSIMLAGAGAIFQEHLGMPYQAGXXXXXXXXXXXXKR 134 HL G + + + L +M+AG G Q + +P+ G Sbjct: 71 EHLMGPWLAKIYDVLIIFYLFSTTMVMIAGGGVTLQMY-KLPFWWGITLICIVTVCLFLW 129 Query: 135 GISGILQINSLVVPLMLTLSLIIIFNTLGVPGAERFLFLPTDHSFFGAWMSPLLYTAFNL 194 + GIL INS+++P+++ L + + + L ++ W + + +T+ N+ Sbjct: 130 DVKGILSINSILIPVLVAGLLYALISFQSTHHHTWTIDLSQQYN----WPASITFTSLNI 185 Query: 195 GMAQAVLVPLARHTNDEQAXXXXXXXXXXXXXXXXXAAHFAMSSQMPGILQYEIPMGTIA 254 A+L + + + + + Q+EIP+ + Sbjct: 186 LSVVAILSSVGKEMKGLGEAKIASVASGLIFGVISFVYNETLVELAGSLSQFEIPLFAV- 244 Query: 255 IRLGPAVQSIYLLL---IFMEIFSTFVADIYGVSIQLQQRXXXXXXXXXXXXXXICYVFS 311 L A ++L + + + I++T VA + G+S +L + F+ Sbjct: 245 --LEGAPYFLFLCMTAVLCLAIYTTTVAGLLGLSSRLMAFIHMPRWMIVLILLVLMVPFT 302 Query: 312 QFGFSSLLSIFYPIFG 327 FGFS L++ YPI+G Sbjct: 303 SFGFSDLIAFLYPIYG 318 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.331 0.143 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,821,685 Number of extensions: 751982 Number of successful extensions: 1664 Number of sequences better than 1.0e-05: 4 Number of HSP's gapped: 1657 Number of HSP's successfully gapped: 4 Length of query: 373 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 265 Effective length of database: 25,922,359 Effective search space: 6869425135 Effective search space used: 6869425135 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 116 (49.3 bits)