BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0569.0027.p (767 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mlr6544 [O] COG0464 ATPases of the AAA+ class 254 3e-67 all3141 [O] COG0464 ATPases of the AAA+ class 215 3e-55 MA2066 [O] COG0464 ATPases of the AAA+ class 106 1e-22 AF1297 [O] COG0464 ATPases of the AAA+ class 100 7e-21 VNG1472G [O] COG0464 ATPases of the AAA+ class 99 3e-20 >mlr6544 [O] COG0464 ATPases of the AAA+ class Length = 782 Score = 254 bits (650), Expect = 3e-67, Method: Compositional matrix adjust. Identities = 129/265 (48%), Positives = 176/265 (66%) Query: 494 LATKIRAMYTWDMLVLPGEQLSQLREICRQVKYRPLVYGEWGFAGRLSLGRGLNILFSGP 553 LA +I ++ D +VLP ++ SQL EI V++ V W F +L GRG+ LF GP Sbjct: 512 LAQRIEPVFQLDEVVLPADRKSQLEEIVDNVRFASRVLDAWKFGDQLPYGRGVTALFHGP 571 Query: 554 PGSGKTMAAEVIATELNLEIYKIDVSQIVSKYIGETEKNLSRIFDEAETSNAILFFDEAD 613 G+GKTMAA +A +LN++I KID+S+IVSKYIG+TEKN+ R+F EA+ S A + DEA+ Sbjct: 572 SGTGKTMAALAVARQLNIQILKIDLSRIVSKYIGDTEKNIDRVFMEAQLSGAAVLVDEAE 631 Query: 614 ALFGKRSEVKDAHDRYANVEISYLLQKMEEYTGIVILATNLNQNLDDAFARRLHFKLEFP 673 L +RSE+ DAHDRYAN+E++YLLQ+ME + G+VIL TN+ QNLD AF RRL F ++FP Sbjct: 632 GLMARRSEINDAHDRYANIEVAYLLQRMEAHEGLVILTTNMRQNLDTAFLRRLRFVIDFP 691 Query: 674 FPEKKQRGLIWRGMFPRGAPLDPDMDYDFMADKFILAGGNIKNIALNAAFYAAHEGCPIG 733 P+ R IWR P G+ + D + +A K L GGN++ I L AAF AA G IG Sbjct: 692 RPDATAREAIWRYCLPDGSHMLDDATFRILARKIELTGGNVRQITLRAAFLAAAAGVLIG 751 Query: 734 MKQIMLAAKREYLKLGRTFLQSDYA 758 +Q++ A+K E KLG ++ D A Sbjct: 752 PEQVLHASKAELAKLGLPTIEIDLA 776 >all3141 [O] COG0464 ATPases of the AAA+ class Length = 448 Score = 215 bits (547), Expect = 3e-55, Method: Compositional matrix adjust. Identities = 113/275 (41%), Positives = 164/275 (59%), Gaps = 4/275 (1%) Query: 478 DLYQACYFQSSR-KIQALATKIRAMYTWDMLVLPGEQLSQLREICRQVKYRPLVYGEWGF 536 DL+ +F S + I A +I W+ LVL + L Q+ ++ + + + +WG Sbjct: 174 DLFTQGHFSSPQFSINFPAQRISTEMEWEDLVLNTQTLDQIHDLKAWITHGSTILYDWGM 233 Query: 537 AGRLSLGRGLNILFSGPPGSGKTMAAEVIATELNLEIYKIDVSQIVSKYIGETEKNLSRI 596 +L LG LF GPPG+GKT+ A ++ N E+YKID+S +VSK+IGETEKNL+ + Sbjct: 234 KRKLKLG--YRALFHGPPGTGKTLTASLLGKYTNKEVYKIDLSMVVSKFIGETEKNLANL 291 Query: 597 FDEAETSNAILFFDEADALFGKRSEVKDAHDRYANVEISYLLQKMEEYTGIVILATNLNQ 656 F +AE+ + ILFFDEADALF KR+ V+DAHD+YAN E+SYLLQ++E Y G+VIL++N Sbjct: 292 FAKAESKDWILFFDEADALFSKRTNVRDAHDKYANQEVSYLLQRVENYDGLVILSSNFKS 351 Query: 657 NLDDAFARRLHFKLEFPFPEKKQRGLIWRGMFPRGAPLDPDMDYDFMADKFILAGGNIKN 716 N+DDAF RR + FP P K+R IW+ FP L ++ +A + L+G +I N Sbjct: 352 NIDDAFIRRFQAIIHFPLPSSKERLQIWQMAFPFQVELAEVVNLWELATTYELSGADIMN 411 Query: 717 IALNAAFYAAHEG-CPIGMKQIMLAAKREYLKLGR 750 + G I + + A KRE+ K G+ Sbjct: 412 VVQYCCLQTLKRGDTVIHQEDLQAAIKREFGKAGK 446 >MA2066 [O] COG0464 ATPases of the AAA+ class Length = 345 Score = 106 bits (265), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 93/299 (31%), Positives = 147/299 (49%), Gaps = 31/299 (10%) Query: 51 QPYTSNHEHLAEELQRLDLRLK----------------LRMLEAPQLDSELALMNEEIRS 94 +PY +N EHL +EL R+DL ++ R L + + E N I Sbjct: 2 KPYANNLEHLLDELSRIDLMVQSYLEKVLESFPGAGDEFRGLYVSEAEIEQIQKNPGIGG 61 Query: 95 LLDAYSADEDDGNLLHVKLSMLEQRIAARLEASSGQEVQLMLPQVASLLNLSGLEVSILV 154 A + ++ + + + I AR S + +L L ++ L L E+ IL+ Sbjct: 62 QQVVADARIERHKVIEAIIEAIREEINARKIESLRKGTELRLHILSELFGLDPFEMDILL 121 Query: 155 ACLAPELDRKYERIYAFLQNDMSDQRPTAGFVLGLFTGSVEERLAARLLFDVNAPLMK-- 212 LAPELD KYE++Y +LQND++ +RPT VL +F ++EE++ AR F +A L K Sbjct: 122 IGLAPELDLKYEKLYPYLQNDVNKKRPTVDLVLSMFIPAIEEKIKAREYFLPDASLRKNH 181 Query: 213 LLLERRGEYGDSRIPLIARPLKLEDWTVNMLLGYEVLDERLAKVASLSTLAIPQQAGF-- 270 L+ GE +S + LI+ +K++D VN LLGY+ LD R+ ++L I + F Sbjct: 182 LIHLLEGE-TNSSLSLISSFIKIDDRIVNFLLGYDDLDTRIGNFSAL----IKSKRSFED 236 Query: 271 ---PEELEQSLLRFVKHYSSGKERRTGSLLYCSGPDEAGKLSGIREVCGKLGLSVLAAD 326 P + + L+ YSS K L++C GP +GK S VC G+++L + Sbjct: 237 LILPADFKSKLMNMAIWYSSNK--LPLKLVFC-GPLGSGKKSAAEAVCRVAGINLLVVN 292 >AF1297 [O] COG0464 ATPases of the AAA+ class Length = 733 Score = 100 bits (250), Expect = 7e-21, Method: Compositional matrix adjust. Identities = 83/258 (32%), Positives = 133/258 (51%), Gaps = 25/258 (9%) Query: 528 PLVYGEWGFAGRLSLGRGLNILFSGPPGSGKTMAAEVIATELNLEIYKIDVSQIVSKYIG 587 PL Y E A + RG IL GPPG+GKT+ A+ +A E N + +++SK++G Sbjct: 474 PLKYPEVFRAANIKPPRG--ILLFGPPGTGKTLLAKAVANESNANFISVKGPELLSKWVG 531 Query: 588 ETEKNLSRIFDEA-ETSNAILFFDEADALFGKRSEVKDAHDRYANVEISYLLQK---MEE 643 E+EK++ +F +A + + ++FFDE D+L +R + D+H +S LL + +EE Sbjct: 532 ESEKHVREMFRKARQVAPCVIFFDEIDSLAPRRGGIGDSH--VTERVVSQLLTELDGLEE 589 Query: 644 YTGIVIL-ATNLNQNLDDAFAR--RLHFKLEFPFPEKKQRGLIWRGMFPRGAPLDPDMDY 700 +V++ ATN +D A R RL + P P+KK R I++ + RG PL D++ Sbjct: 590 LKDVVVIAATNRPDMIDPALLRPGRLERHIYIPPPDKKARVEIFK-IHLRGKPLADDVNI 648 Query: 701 DFMADKFI-LAGGNIKNIALNAAFYAAHEGCPIGM---------KQIMLAAKR--EYLKL 748 + +A+K +G +I+ + A A E GM K++ + K E LK Sbjct: 649 EELAEKTEGYSGADIEAVCREAGMLAIRELIKPGMTREEAKEAAKKLKITKKHFEEALKK 708 Query: 749 GR-TFLQSDYAPYHKLIE 765 R + + D Y KLIE Sbjct: 709 VRPSLTKEDVEKYEKLIE 726 Score = 91.7 bits (226), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 66/200 (33%), Positives = 105/200 (52%), Gaps = 10/200 (5%) Query: 539 RLSLGRGLNILFSGPPGSGKTMAAEVIATELNLEIYKIDVSQIVSKYIGETEKNLSRIFD 598 RL + +L GPPG+GKT+ A+ +A E++ I +I+SKY GE+E+ L IF+ Sbjct: 210 RLGIEPPKGVLLYGPPGTGKTLIAKAVANEVDAHFIPISGPEIMSKYYGESEQRLREIFE 269 Query: 599 EA-ETSNAILFFDEADALFGKRSEVKDAHDRYANVEISYLLQKMEEYTG-IVILATNLNQ 656 EA E + +I+F DE D++ KR EV +R ++ L+ +E IVI ATN Sbjct: 270 EAKENAPSIIFIDEIDSIAPKREEVTGEVERRVVAQLLALMDGLEARGDVIVIAATNRPD 329 Query: 657 NLDDAFAR--RLHFKLEFPFPEKKQRGLIWRGMFPRGAPLDPDMDYDFMA---DKFILAG 711 +D A R R ++E P+K+ R I + R PL D+D + +A + F+ G Sbjct: 330 AIDPALRRPGRFDREIEIGVPDKEGRKEILE-IHTRKMPLAEDVDLEELAELTNGFV--G 386 Query: 712 GNIKNIALNAAFYAAHEGCP 731 +++ + AA +A P Sbjct: 387 ADLEALCKEAAMHALRRVLP 406 >VNG1472G [O] COG0464 ATPases of the AAA+ class Length = 759 Score = 98.6 bits (244), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 71/218 (32%), Positives = 116/218 (53%), Gaps = 12/218 (5%) Query: 513 QLSQLREICRQVKYRPLVYGEWGFAGRLSLGRGLNILFSGPPGSGKTMAAEVIATELNLE 572 +L Q+RE+ P ++ + G + +G +L GPPG+GKT+ A+ +A E++ Sbjct: 204 ELEQVREMIELPMRHPELFQQLG----IDPPKG--VLLHGPPGTGKTLIAKAVANEIDAH 257 Query: 573 IYKIDVSQIVSKYIGETEKNLSRIFDEA-ETSNAILFFDEADALFGKRSEVKDAHDRYAN 631 I +I+SKY GE+E+ L +FDEA E + AI+F DE D++ KR E + +R Sbjct: 258 FETISGPEIMSKYYGESEEKLREVFDEAEENAPAIVFVDELDSIAPKRGETQGDVERRVV 317 Query: 632 VEISYLLQKMEEYTGI-VILATNLNQNLDDAFAR--RLHFKLEFPFPEKKQRGLIWRGMF 688 ++ L+ +E+ + VI ATN +D A R R ++E P++ R I + + Sbjct: 318 AQLLSLMDGLEDRGDVTVIAATNRVDAIDPALRRGGRFDREIEIGVPDQDGRKEILQ-VH 376 Query: 689 PRGAPLDPDMDYDFMADK-FILAGGNIKNIALNAAFYA 725 RG PL D+D D A+ G +I+++A AA A Sbjct: 377 TRGMPLVEDIDLDDYAESTHGFVGADIESLAKEAAMNA 414 Score = 89.7 bits (221), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 93/343 (27%), Positives = 159/343 (46%), Gaps = 31/343 (9%) Query: 409 QIDLPVPDEAARKQAWTTFGQEYRLSPQMDLADFSGSFRFTSGQIRAALAGG-------- 460 +I++ VPD+ RK+ + L +DL D++ S G +LA Sbjct: 358 EIEIGVPDQDGRKEILQVHTRGMPLVEDIDLDDYAESTHGFVGADIESLAKEAAMNALRR 417 Query: 461 --ENIAVWNGSSGAEV--SVSDLYQACYFQSSRKIQALATKIRAMYTWDMLVLPGEQLSQ 516 +I + + AE+ S+S + +A + ++ I+ A + + D L+ Sbjct: 418 VRPDIDLESDEIDAELLESIS-ITEADFKRALNGIEPSALREVFVEVPDTTWADVGGLTD 476 Query: 517 LREICRQVKYRPLVYGEWGFAGRLSLGRGLNILFSGPPGSGKTMAAEVIATELNLEIYKI 576 +E R+ PL Y + + L +L GPPG+GKT+ A+ +A E N + Sbjct: 477 TKERLRETIQWPLDYPD--VFSEMDLQSAKGVLLYGPPGTGKTLLAKAVANEANSNFISV 534 Query: 577 DVSQIVSKYIGETEKNLSRIFDEAETSNA--ILFFDEADALFGKRSEVKDAHDRYANVEI 634 ++++KY+GE+EK + +F++A SNA ++FFDE DA+ G+R V + Sbjct: 535 KGPELLNKYVGESEKGVREVFEKAR-SNAPTVVFFDEIDAIAGQRGRATSDSGVGERV-V 592 Query: 635 SYLLQKM---EEYTGIVILATNLNQNL-DDAFAR--RLHFKLEFPFPEKKQRGLIWRGMF 688 S LL ++ E +V++AT+ +L DDA R RL + P P+ R I + Sbjct: 593 SQLLTELDGIEALEDVVVVATSNRPDLIDDALLRPGRLDRHIHVPVPDADARRAIL-DVH 651 Query: 689 PRGAPLDPDMDYDFMA---DKFILAGGNIKNIALNAAFYAAHE 728 R PL D+D D +A D F+ G +++ + A A E Sbjct: 652 TRDKPLADDVDLDVVAQRMDGFV--GADVEALVREATMNATRE 692 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 53,172,916 Number of extensions: 2211900 Number of successful extensions: 9436 Number of sequences better than 1.0e-05: 117 Number of HSP's gapped: 9324 Number of HSP's successfully gapped: 142 Length of query: 767 Length of database: 38,378,431 Length adjustment: 114 Effective length of query: 653 Effective length of database: 25,230,355 Effective search space: 16475421815 Effective search space used: 16475421815 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 120 (50.8 bits)