BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0569.0005.p (275 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMa0320 [R] COG4221 Short-chain alcohol dehydrogenase of unknown... 130 3e-30 AGc1567 [R] COG4221 Short-chain alcohol dehydrogenase of unknown... 111 1e-24 MA2687 [IQR] COG1028 Dehydrogenases with different specificities... 109 5e-24 alr2142 [R] COG4221 Short-chain alcohol dehydrogenase of unknown... 107 2e-23 VNG0115G [R] COG4221 Short-chain alcohol dehydrogenase of unknow... 107 3e-23 >SMa0320 [R] COG4221 Short-chain alcohol dehydrogenase of unknown specificity Length = 249 Score = 130 bits (326), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 94/264 (35%), Positives = 135/264 (51%), Gaps = 28/264 (10%) Query: 2 LNHKTAVITGASSGIGRAIATRLAAQGVHVFITGRSREQLEEVKSQIELAGGQATCSAFD 61 LN K A++TGASSGIG A A +LA G V I R ++LE++K +IE GG+A D Sbjct: 4 LNGKIALVTGASSGIGAATAAKLAEAGAKVGIAARRTDKLEDLKKKIEAKGGEALVIEMD 63 Query: 62 LRDTGRLQDFIRQTRTQTGHLDIMVNNAGLHIHGNIVDTKVEDWREMIDVNIVALLAGSQ 121 + DT ++ +++ G +DI+VNNAGL +I KV++W+ M+DVN+ LL + Sbjct: 64 VVDTTSVEAGVKKLVDAYGSIDILVNNAGLMPLSDIDQFKVDEWQRMVDVNVKGLLNTTA 123 Query: 122 AAVQAMRLNEGGAPGHIINISS--GNAYEPGRDFYSATKHMVNAIGQALTRELAQE-PIQ 178 A + M GH+ N+SS G G Y ATKH V A L E+ Q+ I+ Sbjct: 124 AVLPQMIKQH---SGHVFNMSSIAGRKVFKGLSVYCATKHAVTAFSDGLRMEVGQKHGIR 180 Query: 179 ITTILPGAVXXXXGRNLEPEAVVQLARKLGMEVEYKQGDHLPEELIARISGAMGQTFLSG 238 +T I PGAV ++ Q Q D L ++ TFL G Sbjct: 181 VTCIQPGAVATELYDHITDPGYRQ------------QMDELATQM----------TFLQG 218 Query: 239 DDIARAVVYALQQPQNVVVRELYL 262 +DI +V+A Q P +V V EL++ Sbjct: 219 EDIGDTIVFAAQAPAHVDVAELFV 242 >AGc1567 [R] COG4221 Short-chain alcohol dehydrogenase of unknown specificity Length = 370 Score = 111 bits (278), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 12/264 (4%), Positives = 39/264 (14%), Gaps = 31/264 (11%) Query: 1 MLNHKTAVITGASSGIGRAIATRLAAQGVHVFITGRSREQLEEVKSQIELAGGQATCSAF 60 + +G A L + + + + + + + Sbjct: 32 TILSHLRNAKPYRTGALGITAAELVSLLELAERGQVNSPEQARQFFETNSVPFRISPAQG 91 Query: 61 DLRDTGRLQDFIRQTRTQTGHLDIMVNNAGLHIHGNIVDTKVEDWREMIDVNIVALLAGS 120 + + + +I + D + A G Sbjct: 92 KSGFVTAFYEPELEVSATPDDVWRYPIYRRPPELVDIDNDNRPDGFDPSYAFGKADEEGI 151 Query: 121 QAAVQAMRLNEGGAPGHIINISS--GNAYEPGRDFYSATKHMVNAIGQALTRELAQEPIQ 178 + F + Sbjct: 152 SYFPDRR---AIDEGCLRGRGLEIAWARSKVDLFFVHVQGAARLVFPDGAIKR--ITYAA 206 Query: 179 ITTILPGAVXXXXGRNLEPEAVVQLARKLGMEVEYKQGDHLPEELIARISGAMGQTFLSG 238 + + E + + + Q Sbjct: 207 KAGHVFSPIGRLLLDRGELDPKTISMQ------TIR------------------QWLADH 242 Query: 239 DDIARAVVYALQQPQNVVVRELYL 262 D V++ + ++ Sbjct: 243 PDEVDGVLWHNRSYIFFREADVAG 266 >MA2687 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 242 Score = 109 bits (272), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 68/207 (32%), Positives = 116/207 (56%), Gaps = 13/207 (6%) Query: 5 KTAVITGASSGIGRAIATRLAAQGVHVFITGRSREQLEEVKSQIELAGGQATCSAFDLRD 64 +TA++TG + GIGRAI LA +GV++ I R+ + +E + Q++ G +A D+R+ Sbjct: 12 QTALVTGGNKGIGRAICFALAKEGVNIIIAARNESESKETQDQLKDMGSKAFEIPVDVRN 71 Query: 65 TGRLQDFIRQTRTQTGHLDIMVNNAGLHIHGNIVDTKVEDWREMIDVNIVALLAGSQAAV 124 ++ I +T + G LDI++NNAG+ + + +T VE++ ++ID N+ + ++ A+ Sbjct: 72 EEDVRRLISRTIDKCGRLDILINNAGVALKKRLEETTVEEYDKIIDTNLKGVFLCTKYAI 131 Query: 125 QAMRLNEGGAPGHIINISSGNAYE--PGRDFYSATKHMVNAIGQALTRELAQEPIQITTI 182 +R ++ G IINISS P Y A+K VN + +A+ EL + I++ +I Sbjct: 132 PYLRRSKN---GKIINISSIGGLHGIPEFSVYCASKFGVNGVTEAIAAELEGQ-IKVYSI 187 Query: 183 LPGAVXXXXGRN-------LEPEAVVQ 202 PGAV R+ L+PE V Q Sbjct: 188 CPGAVDTDMYRSIYKSRPELKPENVAQ 214 >alr2142 [R] COG4221 Short-chain alcohol dehydrogenase of unknown specificity Length = 359 Score = 107 bits (267), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 11/182 (6%), Positives = 31/182 (17%), Gaps = 3/182 (1%) Query: 4 HKTAVITGASSGIGRAIATRLAAQGVHVFITGRSREQLEEVKSQIELAGGQATCSAFDLR 63 + + V LE ++ + LR Sbjct: 6 VVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLETARTLPDADAFGVARLEEALR 65 Query: 64 DTGRLQDFIRQTRTQTGHLDIMVNNAGLHIHGNIVDTKVEDWREMIDVNIVALLAGSQAA 123 + + H H + + + E ++ + Sbjct: 66 LRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVTVWMKLDT 125 Query: 124 VQAMRLNEGGAPGHIINISSGNAYEPGRDFYSATKHMVNAIGQALTRELAQEPIQITTIL 183 + + + + + E ++ T Sbjct: 126 GMHRLGVRPEQAEAFYHRLTQCKN-VRQPVNIVSHFARADEPKCGATE--KQLAIFNTFC 182 Query: 184 PG 185 G Sbjct: 183 EG 184 >VNG0115G [R] COG4221 Short-chain alcohol dehydrogenase of unknown specificity Length = 260 Score = 107 bits (266), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 86/258 (33%), Positives = 130/258 (50%), Gaps = 29/258 (11%) Query: 7 AVITGASSGIGRAIATRLAAQGVHVFITGRSREQLEEVKSQIELAGGQATCSAFDLRDTG 66 A++TGASSGIG A A LAA+G HV + R ++L + +IE G A D+ D Sbjct: 23 AIVTGASSGIGEATAEALAAEGAHVVLAARRADELAALADRIEDTDGDALVVPTDITDDD 82 Query: 67 RLQDFIRQTRTQTGHLDIMVNNAGLHIHGNIVDTKVEDWREMIDVNIVALLAGSQAAVQA 126 + D + +T + G +DI+VNNAG+ + + + R+M++VN++ L+ + A V Sbjct: 83 AIDDLVSETVDEYGRIDILVNNAGVMLLEPLERADRSNLRQMVEVNLLGLMNLTHAVVPI 142 Query: 127 MRLNEGGAPGHIINISS--GNAYEPGRDFYSATKHMVNAIGQALTRELAQEPIQITTILP 184 M+ + GH++N+SS G + Y+ATK VNA A+ +EL + I+ T I P Sbjct: 143 MQDQDA---GHVVNVSSVAGRQADADSSGYNATKFGVNAFTDAIRQELTSQDIRTTIIEP 199 Query: 185 GAVXXXXGRNLEPEAVVQLARKLGMEVEYKQGDHLPEELIARISGAMGQTFLSGDDIARA 244 GA V+ + H+P+E + M L DDIAR Sbjct: 200 GA------------------------VDTELSTHVPDEQVLERFAEMDLPMLHPDDIARG 235 Query: 245 VVYALQQPQNVVVRELYL 262 +VYA QPQ V V EL + Sbjct: 236 IVYATSQPQRVDVNELLI 253 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.134 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,376,741 Number of extensions: 823209 Number of successful extensions: 4309 Number of sequences better than 1.0e-05: 321 Number of HSP's gapped: 3886 Number of HSP's successfully gapped: 321 Length of query: 275 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 170 Effective length of database: 26,268,361 Effective search space: 4465621370 Effective search space used: 4465621370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)