BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0569.0001.p (327 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGc4595 [R] COG2159 Predicted metal-dependent hydrolase of the T... 70 6e-12 >AGc4595 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Length = 117 Score = 69.7 bits (169), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 0/316 (0%), Positives = 0/316 (0%), Gaps = 25/316 (7%) Query: 26 EALLKHAGPNPDGFPTPVWH-----ADLHLEAMEQLGIAVSLLSLSSPHIH-WGDREAAR 79 L A L QL + Sbjct: 15 AVDLAVTCVLIFYLRVDPLVARLPGAAAGLATSWQLNRSNGAELNGFLKNAVTLISRIVG 74 Query: 80 ELARRVNEDGAELVRKYPGKFGLLASLPVPEISDSIVEIKYAMDVLH-----ADGFTLPT 134 Sbjct: 75 134 Query: 135 HALG-VYLGNPCLHDIFAELNTRKAIVALHPNRPALVPEHVLERLP---APMMEFFFDTT 190 Sbjct: 135 194 Query: 191 RTVMNLIFTGTFSRFPDITFIIPHAGAFLPVLADRI--APALHMMPSIFGEHTEDAGVDI 248 Sbjct: 195 -- 252 Query: 249 YTSLKGLYYDIAGACLPRQLDNLLQLIGTDHLLYGSDYPYTPADACKSLADGLEGTNLLT 308 Sbjct: 253 ------ 306 Query: 309 DEARCQIYGENALRLF 324 Sbjct: 307 322 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.140 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,765,945 Number of extensions: 1219350 Number of successful extensions: 12886 Number of sequences better than 1.0e-05: 1 Number of HSP's gapped: 12885 Number of HSP's successfully gapped: 1 Length of query: 327 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 220 Effective length of database: 26,037,693 Effective search space: 5728292460 Effective search space used: 5728292460 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 116 (49.3 bits)