BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0568.0009.p (418 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ECs0532 [GEPR] COG0477 Permeases of the major facilitator superf... 291 1e-78 PA5235 [G] COG2271 Sugar phosphate permease 61 4e-09 PAB0761 [GEPR] COG0477 Permeases of the major facilitator superf... 54 4e-07 YPO0577 [GEPR] COG0477 Permeases of the major facilitator superf... 50 1e-05 >ECs0532 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 406 Score = 291 bits (745), Expect = 1e-78, Method: Compositional matrix adjust. Identities = 167/372 (44%), Positives = 236/372 (63%), Gaps = 7/372 (1%) Query: 26 TIYRILLAVSFVHLFNDSIQALIPAMFPILKDNMLLSYAQVGWIAFALNITSSVIQPVIG 85 T + IL A+S HL ND IQ+LI A++P+L+ L++ Q+G I + SS++QPV+G Sbjct: 22 TSFGILGAISLSHLLNDMIQSLILAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVG 81 Query: 86 YAADRTPRPILLPLGMCCTFAGVFLLAFAGNYALVIFSVMLVGFGSAAFHPEGMRVAHMA 145 Y D+ P P LP+GMC T +G+ LLA AG++ V+ + LVG GS+ FHPE RVA MA Sbjct: 82 YWTDKYPMPWSLPIGMCFTLSGLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMA 141 Query: 146 AGQRKGLSQSIFQVGGNAGQSLGPLLMKWVFIPFGQMGALGFTVIAAAGIAVQTYVAKWY 205 +G R GL+QSIFQVGGN G SLGPLL + P+G+ F + A I V +++WY Sbjct: 142 SGGRHGLAQSIFQVGGNFGSSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWY 201 Query: 206 --RKMLNEGYTFRKKSAARTIDPARSKSILTATIILVFLVFVRSWYGASIGGYYAFYLMK 263 + +N+G K A I+P ++ A IL+ L+F + +Y ASI YY FYLM+ Sbjct: 202 SAQHRMNKG-----KPKATIINPLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQ 256 Query: 264 NYGMTIDDAQIYIFMYLAAGAVGTFFGGPLADRFGRRNLILLSMVGTVPFALALPFVNQT 323 +G++I +AQ+++F +L A A GT GGP+ D+ GR+ +I S++G PF L LP+ + Sbjct: 257 KFGLSIQNAQLHLFAFLFAVAAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLH 316 Query: 324 FALVLLVISGFILLSSFSVTVIYAQMLYPGNIGTVSGLITGLAFGLGGIGSVVIGELIDR 383 + VL VI GFIL S+FS ++YAQ L PG IG VSGL G AFG+GG+G+ V+G + D Sbjct: 317 WTGVLTVIIGFILASAFSAILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADH 376 Query: 384 IGITTVFVACGF 395 I V+ C F Sbjct: 377 TSIELVYKICAF 388 >PA5235 [G] COG2271 Sugar phosphate permease Length = 448 Score = 60.8 bits (146), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 95/418 (22%), Positives = 169/418 (40%), Gaps = 47/418 (11%) Query: 4 KTISSTAPIIKEEQDQ--RHLQQATIYRILLAVSFVHLFNDSIQALIPAMFPILKDNMLL 61 + AP+ E D R L+ I + + +L + +P + ++D Sbjct: 6 RPAPHQAPLPDAEVDSTYRRLRWQIFAGIFIGYAGYYLLRKNFSLAMPYL---IEDGY-- 60 Query: 62 SYAQVGWIAFALNITSSVIQPVIGYAADRTPRPILLPLGMCCTFAGVFLLAFAGNYALVI 121 S Q+G A+ I + + ++G +DR+ + LP G+ + A +F+ FA ++A Sbjct: 61 SRGQLGVAMSAIAIAYGLSKFIMGLVSDRSNPRVFLPFGLLVSAAVMFIFGFA-HWATSS 119 Query: 122 FSVMLV-----GFGSA-AFHPEGMRVAHMAAGQRKGLSQSIFQVGGNAGQSL-GPLLMKW 174 ++M V G+ + P G + H + + +G S++ V N G L GPL + Sbjct: 120 VAIMFVLLFINGWAQGMGWPPSGRTMVHWWSQKERGSVVSVWNVAHNVGGGLIGPLFLLG 179 Query: 175 VFIPFGQMGALGFTVIAAAGIAVQTYVA-------------KWYRKMLNEGYTFRKKSAA 221 + + A I A G+AV Y A + ++ EGY + Sbjct: 180 LALFGDWRSAFYVPAIVAVGVAVFAYFAMRDTPQSCGLPPIEKHKDDYPEGYDEHHEREL 239 Query: 222 RTID-----PARSKSILTATIILVFLVFVRSWYGASIGGYYAFYLMKNYGMTIDDAQIYI 276 T + R+K + + VF+ +R YG + + YL + +D + Sbjct: 240 STKEIFVTYVLRNKLLWYIALANVFVYLLR--YG--VLDWAPTYLKEAKHFNVDKSSWAY 295 Query: 277 FMYLAAGAVGTFFGGPLADRFGRRN-------LILLSMVGTVPFALALPFVNQTFALVLL 329 F Y AG GT G L+D+ + N ++L VGT+ + L P N + L Sbjct: 296 FFYEWAGIPGTLLCGWLSDKLFKGNRGATGVVFMILVTVGTLIYWLN-PAGNPAIDMAAL 354 Query: 330 VISGFILLSSFSVTVIYAQMLYPGNIGTVSGLITGLAFGLGG--IGSVVIGELIDRIG 385 ++ GF++ + + A L P + TGL LGG + ++G +D G Sbjct: 355 IMIGFLIYGPVMLIGLQALELAPKKAAGTAAGFTGLFGYLGGSVAANALVGYTVDHFG 412 >PAB0761 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 372 Score = 54.3 bits (129), Expect = 4e-07, Method: Compositional matrix adjust. Identities = 78/354 (22%), Positives = 151/354 (42%), Gaps = 33/354 (9%) Query: 39 LFNDSIQALIPAMFPILKDNMLLSYAQVGWIAFALNITSSVIQPVIGYAADRTPRPILLP 98 +FN S + +P++ PI+ ++ ++ A++G + +L + S+IQ GY D+ R LL Sbjct: 15 IFNYSHRMAVPSLAPIIMKDLGINNAEIGLLMTSLLLPYSLIQVPAGYIGDKIGRKKLLT 74 Query: 99 LGMCCTFAGVFLLAFAGNYALVIFSVMLVGFGSAAFHPEGMRVAHMAAGQRKGLSQSIFQ 158 + + L+ +Y ++ L GF + ++ + +RKG + F Sbjct: 75 ISILGYSLSSALIVLTRDYWDLVTVRALYGFFAGLYYAPATALISELFRERKGSALGFFM 134 Query: 159 VGGNAGQSLGPLLMKWVFIPFGQMGALGFTVIAAAGIAVQTYVAKWYRKMLNEGYTFRKK 218 VG G + PL++ V + A I ++ + + VA + EG F+ Sbjct: 135 VGPAIGSGITPLIVVPVALTLSWRYAFLVLSIMSSIVGILLMVAIKGEPIKVEGVKFKIP 194 Query: 219 SAARTIDPARSKSILTATIILVFLVFVRSWYGASIGGYYAF------YLMKNYGMTIDDA 272 VFL+ + ++ G +G ++A YL+ G+ ++ A Sbjct: 195 RG-------------------VFLLSLANFLG--LGAFFAMLTFLVSYLVSR-GVGMEKA 232 Query: 273 QIYIFMYLAAGAVGTFFGGPLADRFGRRNLILLSMVGTVPFALALPFVNQTFALVLLVIS 332 + M G +G+ G L D G+ +++L + ++ L + + F + L Sbjct: 233 SLMFSMLSLVGILGSIIAGFLYDHLGKVSVLLAYALNSLLTFLVIVIPSPLFLIPL---- 288 Query: 333 GFILLSSFSVTVIY-AQMLYPGNIGTVSGLITGLAFGLGGIGSVVIGELIDRIG 385 G +L S + Y ++ N+G V G + + F IG ++G LIDR+G Sbjct: 289 GLVLYSVGGIMTAYTSEKASRENLGVVMGFVNMVGFFGATIGPYIVGFLIDRLG 342 >YPO0577 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 434 Score = 49.7 bits (117), Expect = 1e-05, Method: Compositional matrix adjust. Identities = 78/373 (20%), Positives = 156/373 (41%), Gaps = 43/373 (11%) Query: 53 PILKDNMLLSYAQVGWIAFALNITSSVIQPVIGYAADRTPRPILLPLGMCCTFAGVFLL- 111 P L++ + ++ Q +I A + +++QPV GY D + M +F + Sbjct: 35 PTLQEQLHITTQQYSYIIAAYSAAYTLMQPVAGYVLDVMGTKV--GYAMFAVMWAIFCMS 92 Query: 112 -AFAGNYALVIFSVMLVGFGSAAFHPEGMRV-AHMAAGQRKGLSQSIFQVGGNAGQSLGP 169 A A ++ + + VG AA P G++ + + + ++ F VG + G + P Sbjct: 93 TALASSWGGLAIARGAVGMAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGGMIAP 152 Query: 170 LLMKWVFIPFGQMGALGFT----VIAAAGIAVQTYVAKWYRKMLNE--GYTFRKKSAART 223 L+ W + A T +I A + K +++ +E Y + A + Sbjct: 153 PLVVWAIVMHSWQMAFIITGVLSLIWAIAWLILYKHPKDQKRLSDEEREYILSGQEAQHS 212 Query: 224 IDPARSKSILTATIILVFLVFVRSWYGASIGGYYA------------FYLMKNYGMTIDD 271 A+ S + ++ R ++G +I + A ++ K YG + + Sbjct: 213 TANAKKMSAMQ-------IIRNRQFWGIAIPRFLAEPAWGTFNAWIPLFMFKAYGFNLKE 265 Query: 272 AQIYIFMYLAAGAVGTFFGG---PLADRFGRRNLI-----LLSMVGTV---PFALALPFV 320 ++ +M + VG GG PL ++ + NLI +++M G + P + L F Sbjct: 266 IAMFAWMPMLFADVGCILGGYLPPLFQKYFKVNLIVSRKLVVTMGGVLMIGPGTIGL-FT 324 Query: 321 NQTFALVLLVISGFILLS-SFSVTVIYAQMLYPGNIGTVSGLITGLAFGLGGIGSVVIGE 379 + A+ LL I GF + S ++ + + + + T +GL A+ + ++V+G Sbjct: 325 SPYAAIALLCIGGFAHQALSGALITLSSDVFGRNEVATANGLTGMAAWTASTLFALVVGA 384 Query: 380 LIDRIGITTVFVA 392 L D +G + +F A Sbjct: 385 LADTMGFSPLFAA 397 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.142 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,180,738 Number of extensions: 1116518 Number of successful extensions: 4676 Number of sequences better than 1.0e-05: 28 Number of HSP's gapped: 4669 Number of HSP's successfully gapped: 28 Length of query: 418 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 309 Effective length of database: 25,807,025 Effective search space: 7974370725 Effective search space used: 7974370725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 117 (49.7 bits)