BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0567.0031.p (304 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yceE [T] COG2310 Uncharacterized proteins involved in stress ... 186 3e-47 STM0382 [GEPR] COG0477 Permeases of the major facilitator superf... 179 6e-45 SPy0501 [GEPR] COG0477 Permeases of the major facilitator superf... 165 9e-41 SP0972 [GEPR] COG0477 Permeases of the major facilitator superfa... 160 2e-39 L129379 [GEPR] COG0477 Permeases of the major facilitator superf... 155 6e-38 >BS_yceE [T] COG2310 Uncharacterized proteins involved in stress response, homologs of TerZ and putative cAMP-binding protein CABP1 Length = 408 Score = 186 bits (473), Expect = 3e-47, Method: Compositional matrix adjust. Identities = 109/301 (36%), Positives = 178/301 (59%), Gaps = 1/301 (0%) Query: 1 MGFVHNVYILIGLRLLQGTITGYGTACTTLIATQTDKEHAGYALGTLSTASIAGSLLGPS 60 MG N++ + LR L G + G+ LIATQ + +G+ALGTLST ++G+LLGP Sbjct: 101 MGLAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM 160 Query: 61 LGGFIAENMGLEDXXXXXXXXXXXXXXXXXXXVQESFVR-EDKQALRFKEVWSSVPEKNL 119 GG +A++ GL ++E F K+ L +EV +S+ L Sbjct: 161 AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNPKL 220 Query: 120 TITLFVSFFVLTVALYTVEPVITVYVNHLSRSGSHIALIAGLTFSASGLANIIAAPRLGR 179 ++LFV+ ++ VA ++ P++T+YV L+ + S++A I+G+ S G+A +++APRLG+ Sbjct: 221 VLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAPRLGK 280 Query: 180 LSDRIGAHRVMLAALITAGLIFIPQAFVQNPWQLMGLRFVLGLAAGGLIPSVNILVKKIT 239 L DRIG ++++ ALI + L+ IP ++VQ P QL LRF+LG A G L+P+V L+ + Sbjct: 281 LGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQTLLVYNS 340 Query: 240 PSSITGRVFGLNMSAGYLGIFGGSVLGGQIAAWFGIRYVFFITAALLLINAVWVYFKVYK 299 + I GR+F N S +G G ++G I+A +G R VF +TA ++L NAV+ + + + Sbjct: 341 SNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAVYSWNSLRR 400 Query: 300 K 300 + Sbjct: 401 R 401 Score = 68.9 bits (167), Expect = 8e-12, Method: Compositional matrix adjust. Identities = 58/188 (30%), Positives = 99/188 (52%), Gaps = 3/188 (1%) Query: 116 EKNLTITLFVSFFVLTVALYTVEPVITVYVNHLSRSG-SHIALIAGLTFSASGLANIIAA 174 ++NL I ++ F+ A V P + +YV L +G S + + +G+ FS + L + IA+ Sbjct: 13 KRNL-IVAWLGCFLTGAAFSLVMPFLPLYVEQLGVTGHSALNMWSGIVFSITFLFSAIAS 71 Query: 175 PRLGRLSDRIGAHRVMLAALITAGLIFIPQAFVQNPWQLMGLRFVLGLAAGGLIPSVNIL 234 P G L+DR G ++L + + G++ + QN WQ + LR +L GG +P+ N L Sbjct: 72 PFWGGLADRKGRKLMLLRSALGMGIVMVLMGLAQNIWQFLILRALL-GLLGGFVPNANAL 130 Query: 235 VKKITPSSITGRVFGLNMSAGYLGIFGGSVLGGQIAAWFGIRYVFFITAALLLINAVWVY 294 + P + +G G + G G G + GG +A +G+R VFFITA++L++ Sbjct: 131 IATQVPRNKSGWALGTLSTGGVSGALLGPMAGGLLADSYGLRPVFFITASVLILCFFVTL 190 Query: 295 FKVYKKLN 302 F + +K Sbjct: 191 FCIREKFQ 198 >STM0382 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 403 Score = 179 bits (453), Expect = 6e-45, Method: Compositional matrix adjust. Identities = 108/305 (35%), Positives = 178/305 (58%), Gaps = 2/305 (0%) Query: 2 GFVHNVYILIGLRLLQGTITGYGTACTTLIATQTDKEHAGYALGTLSTASIAGSLLGPSL 61 F +V+ L LR + G +GY L+A+Q +E +G+AL TLSTA I+G + GP + Sbjct: 94 AFATHVWQLFLLRGIMGLTSGYIPNAMALVASQVPRERSGWALSTLSTAQISGVIGGPLM 153 Query: 62 GGFIAENMGLEDXXXXXXXXXXXXXXXXXXXVQESFVREDKQALRF--KEVWSSVPEKNL 119 GGF+A+++GL ++E K++ R K V++S+P L Sbjct: 154 GGFVADHIGLRAVFLITAMLLVVSFLVTLFLIKEGVRPVIKKSERLSGKAVFASLPYPAL 213 Query: 120 TITLFVSFFVLTVALYTVEPVITVYVNHLSRSGSHIALIAGLTFSASGLANIIAAPRLGR 179 I+LF + V+ + ++ P++ +++ + ++IA ++GL S G++ +I+APRLG+ Sbjct: 214 VISLFFTTMVIQLCNGSISPILALFIKSMMPDSNNIAFLSGLIASVPGISALISAPRLGK 273 Query: 180 LSDRIGAHRVMLAALITAGLIFIPQAFVQNPWQLMGLRFVLGLAAGGLIPSVNILVKKIT 239 L DRIG R+++A LI A L+F ++V P+QL LRF+LG A G ++P+V L+ K + Sbjct: 274 LGDRIGTERILMATLICAVLLFFAMSWVTTPFQLGLLRFLLGFADGAMLPAVQTLLVKYS 333 Query: 240 PSSITGRVFGLNMSAGYLGIFGGSVLGGQIAAWFGIRYVFFITAALLLINAVWVYFKVYK 299 ITGR+FG N S YLG G ++G ++A G R+VF TAA++LIN + + + Sbjct: 334 SDQITGRIFGYNQSFMYLGNVVGPLMGATVSAMAGFRWVFIATAAIVLINIGQLTLALRR 393 Query: 300 KLNSQ 304 + N+Q Sbjct: 394 RRNAQ 398 Score = 80.5 bits (197), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 64/183 (34%), Positives = 97/183 (53%), Gaps = 9/183 (4%) Query: 109 EVWSSVPEKNLTITLFVSFFVLTVALYTVEPVITVYVNHLSRSGSHIAL--IAGLTFSAS 166 E W + NL F FF +A+ + P + +Y++ L S SH AL +GLTFS + Sbjct: 2 ESW----KVNLISVWFGCFFT-GLAISQILPFLPLYISQLGVS-SHEALSMWSGLTFSIT 55 Query: 167 GLANIIAAPRLGRLSDRIGAHRVMLAALITAGLIFIPQAFVQNPWQLMGLRFVLGLAAGG 226 L + I +P G L+DR G ++L A + + + QAF + WQL LR ++GL + G Sbjct: 56 FLISAIVSPMWGSLADRKGRKLMLLRASLGMAIAILLQAFATHVWQLFLLRGIMGLTS-G 114 Query: 227 LIPSVNILVKKITPSSITGRVFGLNMSAGYLGIFGGSVLGGQIAAWFGIRYVFFITAALL 286 IP+ LV P +G +A G+ GG ++GG +A G+R VF ITA LL Sbjct: 115 YIPNAMALVASQVPRERSGWALSTLSTAQISGVIGGPLMGGFVADHIGLRAVFLITAMLL 174 Query: 287 LIN 289 +++ Sbjct: 175 VVS 177 >SPy0501 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 397 Score = 165 bits (417), Expect = 9e-41, Method: Compositional matrix adjust. Identities = 105/310 (33%), Positives = 166/310 (53%), Gaps = 12/310 (3%) Query: 1 MGFVHNVYILIGLRLLQGTITGYGTACTTLIATQTDKEHAGYALGTLSTASIAGSLLGPS 60 + + NV+ L+ LRLL G GY T LIA+Q KE +GYALGTL+T AG+L+GP Sbjct: 91 LAIIPNVFWLLILRLLTGVSAGYVPNATALIASQAPKEESGYALGTLATGVTAGALIGPL 150 Query: 61 LGGFIAENMGLEDXXXXXXXXXXXXXXXXXXXVQESFVREDKQALRF-----KEVWSSVP 115 LGG +AE +G+ V+E F K RF K + V Sbjct: 151 LGGILAELLGIRQVFLLVGVILFLCSLMTAVYVKEEF----KPVRRFEMIPTKVILKQVK 206 Query: 116 EKNLTITLFVSFFVLTVALYTVEPVITVYVNHLSRSGSHIALIAGLTFSASGLANIIAAP 175 + + LFV+ ++ ++ +V P++++Y+ HL ++ ++ +GL SA G +++ ++ Sbjct: 207 SPQIMLGLFVTSMIIQISAQSVAPILSLYIRHLGQT-HNLMFTSGLVVSAMGFSSLFSSS 265 Query: 176 RLGRLSDRIGAHRVMLAALITAGLIFIPQAFVQNPWQLMGLRFVLGLAAGGLIPSVNILV 235 LG+L DR G HR++LAAL + +++ A Q +QL LRF G G L+PS+N L+ Sbjct: 266 YLGKLGDRFGNHRLLLAALCYSFIMYFSSALAQTSFQLGVLRFAYGFGVGALMPSINSLL 325 Query: 236 KKITPSSITGRVFGLNMSAGYLGIFGGSVLGGQIAAWFGIRYVFFITAALLLINAVW--V 293 K+TP RVF N LG G +G +A G R VF++T+ ++ +N +W + Sbjct: 326 TKLTPKEGISRVFAYNQMFSNLGQVIGPFIGSNVAVVLGYRSVFYVTSLIVFVNLIWSLI 385 Query: 294 YFKVYKKLNS 303 F+ Y K+ Sbjct: 386 IFRKYIKVKD 395 Score = 63.5 bits (153), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 57/187 (30%), Positives = 87/187 (46%), Gaps = 16/187 (8%) Query: 116 EKNLTITLFVSFFVLTVALYTVEPVITVYVNHLSRSGSHIALIAGLTFSASGLANIIAAP 175 +NL + +FF + V P + +YV +L + AGL + + LA+ + AP Sbjct: 4 RQNLKVAWLGNFFT-GASFSLVMPFMALYVENLGTPTELVEYYAGLAVAVTALASALFAP 62 Query: 176 RLGRLSDRIGAHRVMLAA-----LITAGLIFIPQAFVQNPWQLMGLRFVLGLAAGGLIPS 230 G+L+DR G +ML A GL IP F W L+ LR + G++A G +P+ Sbjct: 63 VWGKLADRYGRKPMMLRASFVMTFTMGGLAIIPNVF----WLLI-LRLLTGVSA-GYVPN 116 Query: 231 VNILVKKITPSSITGRVFGLNMSAGYLGIFGGSVLGGQIAAWFGIRYVFFITAALL---- 286 L+ P +G G + G G +LGG +A GIR VF + +L Sbjct: 117 ATALIASQAPKEESGYALGTLATGVTAGALIGPLLGGILAELLGIRQVFLLVGVILFLCS 176 Query: 287 LINAVWV 293 L+ AV+V Sbjct: 177 LMTAVYV 183 >SP0972 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 399 Score = 160 bits (405), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 102/284 (35%), Positives = 160/284 (56%), Gaps = 2/284 (0%) Query: 1 MGFVHNVYILIGLRLLQGTITGYGTACTTLIATQTDKEHAGYALGTLSTASIAGSLLGPS 60 + FV N+Y LI LRLL G G+ T LIA+Q KE +G ALGTLST +AG+L GP Sbjct: 94 LAFVPNIYWLIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPF 153 Query: 61 LGGFIAENMGLEDXXXXXXXXXXXXXXXXXXXVQESFVREDKQ-ALRFKEVWSSVPEKNL 119 +GGFIAE G+ ++E F K+ A+ KE+++SV L Sbjct: 154 IGGFIAELFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYL 213 Query: 120 TITLFVSFFVLTVALYTVEPVITVYVNHLSRSGSHIALIAGLTFSASGLANIIAAPRLGR 179 + LF++ FV+ + ++ P++ +YV L ++ ++ ++GL S+ G +++++A +G+ Sbjct: 214 LLNLFLTSFVIQFSAQSIGPILALYVRDLGQT-ENLLFVSGLIVSSMGFSSMMSAGVMGK 272 Query: 180 LSDRIGAHRVMLAALITAGLIFIPQAFVQNPWQLMGLRFVLGLAAGGLIPSVNILVKKIT 239 L D++G HR+++ A + +I++ A +P QL RF+ GL G LIP VN L+ K+T Sbjct: 273 LGDKVGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMT 332 Query: 240 PSSITGRVFGLNMSAGYLGIFGGSVLGGQIAAWFGIRYVFFITA 283 P + RVF N YLG G + G +A FG VF+ T+ Sbjct: 333 PKAGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATS 376 Score = 66.2 bits (160), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 51/176 (28%), Positives = 84/176 (47%), Gaps = 2/176 (1%) Query: 116 EKNLTITLFVSFFVLTVALYTVEPVITVYVNHLSRSGSHIALIAGLTFSASGLANIIAAP 175 + NL I F +F ++ V P + ++V +L +A AGL S S ++ + +P Sbjct: 7 KDNLRIAWFGNFLT-GASISLVVPFMPIFVENLGVGSQQVAFYAGLAISVSAISAALFSP 65 Query: 176 RLGRLSDRIGAHRVMLAALITAGLIFIPQAFVQNPWQLMGLRFVLGLAAGGLIPSVNILV 235 G L+D+ G +M+ A + + AFV N + L+ LR + G+ A G +P+ L+ Sbjct: 66 IWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYWLIFLRLLNGVFA-GFVPNATALI 124 Query: 236 KKITPSSITGRVFGLNMSAGYLGIFGGSVLGGQIAAWFGIRYVFFITAALLLINAV 291 P +G G + G G +GG IA FGIR VF + + L + A+ Sbjct: 125 ASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAELFGIRTVFLLVGSFLFLAAI 180 >L129379 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 398 Score = 155 bits (393), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 95/302 (31%), Positives = 158/302 (52%), Gaps = 2/302 (0%) Query: 1 MGFVHNVYILIGLRLLQGTITGYGTACTTLIATQTDKEHAGYALGTLSTASIAGSLLGPS 60 M FV + L+ LR+L G +G+ + L+A+ KE +G L L+T S+AG+L+GP Sbjct: 94 MAFVTAAWQLLILRMLLGAFSGFVSNSMALMASSAPKEKSGAVLSLLTTGSVAGTLIGPI 153 Query: 61 LGGFIAENMGLEDXXXXXXXXXXXXXXXXXXXVQESFVR-EDKQALRFKEVWSSVPEKNL 119 +GG + G V+E F E K L +K+VW V + Sbjct: 154 IGGILVNITGYRRVFSVTGIIMFLVFFLALFFVKEIFKPVEKKNMLSYKQVWEVVTHPAI 213 Query: 120 TITLFVSFFVLTVALYTVEPVITVYVNHLSRSGSHIALIAGLTFSASGLANIIAAPRLGR 179 +F + + + ++ PV+++YV L +I +AG+ +A G+ ++AAP LGR Sbjct: 214 IWGMFFATLITQMTNQSINPVLSLYVQELMHGQGNITFMAGIVAAAPGIVTLVAAPFLGR 273 Query: 180 LSDRIGAHRVMLAALITAGLIFIPQAFVQNPWQLMGLRFVLGLAAGGLIPSVNILVKKIT 239 L D++G +++ L + ++F A N W L+ +R ++G++ ++PSV ++ K + Sbjct: 274 LGDKVGQRKILGFGLFFSLIVFSITAMTTNVWFLIVMRLLVGVSDAAILPSVQAILAKES 333 Query: 240 PSSITGRVFGLNMSAGYLGIFGGSVLGGQIAAWFGIRYVFFITAALLLINAVWVYFKVYK 299 P +ITGR+F N SA +G F G +LG IA + RYVF +A L++ N + YF K Sbjct: 334 PQAITGRIFSYNQSAQSIGAFAGPLLGSAIAGFIDYRYVFVGSAILVVFNLI-NYFTHTK 392 Query: 300 KL 301 L Sbjct: 393 SL 394 Score = 62.4 bits (150), Expect = 8e-10, Method: Compositional matrix adjust. Identities = 52/180 (28%), Positives = 89/180 (49%), Gaps = 7/180 (3%) Query: 108 KEVWSSVPEKNLTITLFVSFFVLTVALYTVEPVITVYVNHLSR-SGSHIALIAGLTFSAS 166 KE W ++NL I L+ F+ + + P I++Y++ L S + + + +GL FS++ Sbjct: 2 KESW----QRNL-IVLWFGTFMTGIGSSLIAPFISLYISTLGHYSKTELNIWSGLVFSST 56 Query: 167 GLANIIAAPRLGRLSDRIGAHRVMLAALITAGLIFIPQAFVQNPWQLMGLRFVLGLAAGG 226 + I +P GRL+D+ G ++L A + + AFV WQL+ LR +LG A G Sbjct: 57 FIVLAIVSPLWGRLADQKGRKLMLLRASLGMAISISLMAFVTAAWQLLILRMLLG-AFSG 115 Query: 227 LIPSVNILVKKITPSSITGRVFGLNMSAGYLGIFGGSVLGGQIAAWFGIRYVFFITAALL 286 + + L+ P +G V L + G G ++GG + G R VF +T ++ Sbjct: 116 FVSNSMALMASSAPKEKSGAVLSLLTTGSVAGTLIGPIIGGILVNITGYRRVFSVTGIIM 175 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.325 0.141 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,210,554 Number of extensions: 752281 Number of successful extensions: 10651 Number of sequences better than 1.0e-05: 45 Number of HSP's gapped: 10676 Number of HSP's successfully gapped: 53 Length of query: 304 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 198 Effective length of database: 26,153,027 Effective search space: 5178299346 Effective search space used: 5178299346 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 115 (48.9 bits)