BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0567.0020.p (334 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SA0615 [T] COG0642 Signal transduction histidine kinase 224 2e-58 lin1852 [T] COG0642 Signal transduction histidine kinase 142 6e-34 CAC0225 [T] COG0642 Signal transduction histidine kinase 129 5e-30 SP1632 [T] COG0642 Signal transduction histidine kinase 129 6e-30 CAC0372 [T] COG0642 Signal transduction histidine kinase 127 3e-29 >SA0615 [T] COG0642 Signal transduction histidine kinase Length = 346 Score = 224 bits (570), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 115/326 (35%), Positives = 187/326 (57%), Gaps = 1/326 (0%) Query: 5 YIKEKLSWXXXXXXXXXXXXXVAYVDSSIPFLSVLYIVVLNIMVCTTFVFLRYHKETRFY 64 ++K +++W ++ +D P S+ YIV LN+ + F+ L Y KE + Y Sbjct: 10 FLKSRMNWIFWILFLNLLMLGISLIDYDFPIDSLFYIVSLNLSLTMIFLILTYFKEVKLY 69 Query: 65 QRMGAWDEVYDLSTFKEANSPFERMVQEAVNAQTERYRRESSANFQLLEQEKDDLLSWIH 124 + E+ ++ A +PF+R + + Q ++ + L + + ++H Sbjct: 70 KHFDKDKEIEEIKHKDLAETPFQRHTVDYLYRQISAHKEKVVEQQLQLNMHEQTITEFVH 129 Query: 125 EVKTPLTAMQLMIERLTDETLKPKLMYEWLRVHHLLDQQLHQKRIPFMRNDLYIEMIRLE 184 ++KTP+TAM+L+I++ ++ K L+YEW R++ +LD QL+ R+ R D+Y + + L+ Sbjct: 130 DIKTPVTAMKLLIDQEKNQERKQALLYEWSRINSMLDTQLYITRLESQRKDMYFDYVSLK 189 Query: 185 PVLNKEIQALKSWCIPKGIGFDVDLAV-EAVLTDGKWLGFILRQLLTNAVKYSENSDILI 243 ++ EIQ + KGIGFDVD V + V TD KW I+RQ+L+NA+KYSEN +I I Sbjct: 190 RMVIDEIQLTRHISQVKGIGFDVDFKVDDYVYTDTKWCRMIIRQILSNALKYSENFNIEI 249 Query: 244 QSREKAGHTVLIIEDHGRGIDPKDLPRIYDKGFTSTTGRQEGAATGMGLYLTKQVAEPLQ 303 + H L I+D+GRGI KD+PRI+++GFTST R E ++GMGLYL V + L Sbjct: 250 GTELNDQHVSLYIKDYGRGISKKDMPRIFERGFTSTANRNETTSSGMGLYLVNSVKDQLG 309 Query: 304 IGIHADSMLGEGTTFTLTFPRKNDFL 329 I + S +G+GTT L FP +N+ + Sbjct: 310 IHLQVTSTVGKGTTVRLIFPLQNEIV 335 >lin1852 [T] COG0642 Signal transduction histidine kinase Length = 182 Score = 142 bits (359), Expect = 6e-34, Method: Compositional matrix adjust. Identities = 0/297 (0%), Positives = 0/297 (0%), Gaps = 15/297 (5%) Query: 51 TFVFLRYHKETRFYQRMGAWDEVYDLSTFK-EANS----PFERMVQEAVNAQ--TERYRR 103 K + + + + Sbjct: 48 VTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDFRYRAT 107 Query: 104 E--SSANFQLLEQEKDD-LLSWIHEVKTPLTAMQLMIER--LTD-ETLKPKLMYEWLRVH 157 + ++ + + + T + ++ Sbjct: 108 DPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSPWMVMQAT 167 Query: 158 HLLDQQLHQKRIPFMRNDLYIEMIRLEPVLNKEIQALKSWCIPKGIGFDVDLAVEAVLTD 217 Sbjct: 168 227 Query: 218 GKWLGFILRQLLTNAVKY-SENSDILIQS-REKAGHTVLIIEDHGRGIDPKDLPRIYDKG 275 Sbjct: 228 287 Query: 276 FTSTTGRQEGAATGMGLYLTKQVAEPLQIGIHADSMLGEGTTFTLTFPRKNDFLRIT 332 Sbjct: 288 344 >CAC0225 [T] COG0642 Signal transduction histidine kinase Length = 339 Score = 129 bits (325), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 94/340 (27%), Positives = 170/340 (50%), Gaps = 14/340 (4%) Query: 4 KYIKEKLSWXXXXXXXXXXXXXVAYVDSSIPFLS--VLYIVVLNIMVCTTFVFLRYHKET 61 +Y+K+ + Y+D LS + Y++ + + F+ + Y + Sbjct: 5 RYLKDNFRFILLYVLLGAFVSAFTYLDEKNRMLSSNIGYMIFVMFFILLIFLCVDYSIKN 64 Query: 62 RFYQRMGAWDEVYDLSTFKEANSPFERMVQEAVNAQTERYRRESSANFQLLEQEKDD--- 118 + +++ A E+ D + P E + ++ + Y+ + + LE+E +D Sbjct: 65 KHIKKLIANGEIKDKTPI--LPEPLEYKDEVYISIIEDLYK-DYNERIVSLEKEFEDNNE 121 Query: 119 -LLSWIHEVKTPLTAMQLMIERLTDETLKPKLMYEWLRVHHLLDQQLHQKRIPFMRNDLY 177 + +WIHE+KTP+ +L++E + E M E ++ +++ L+ R D Sbjct: 122 FMTAWIHEIKTPIATAKLLLE--SGEIDSQLFMEEIEKIDDYVEKVLYYSRSDNFSKDYI 179 Query: 178 IEMIRLEPVLNKEIQALKSWCIPKGIGFDVDLAVE-AVLTDGKWLGFILRQLLTNAVKYS 236 I + + ++ + I++ I K I +++ V TD KW FIL Q+++NA+KY+ Sbjct: 180 ISEVNISKLIKECIKSHSKIFIKKHIKLKLEIDERFNVETDKKWFLFILNQIISNALKYT 239 Query: 237 EN-SDILIQSREKAGHTVLIIEDHGRGIDPKDLPRIYDKGFTSTTGRQEGA-ATGMGLYL 294 + +I+I++ E V+IIED+G GI +DL RI+ K FT GR+E + ATGMGLYL Sbjct: 240 NDMGNIIIKAFEDDNEKVVIIEDNGIGIKKEDLDRIFSKSFTGYNGRKENSKATGMGLYL 299 Query: 295 TKQVAEPLQIGIHADSMLGEGTTFTLTFPRKNDFLRITGM 334 + ++AE L I +S EGT + FP+ D+ +T M Sbjct: 300 SNKLAEKLGHNITIESKYNEGTKLYIHFPKWCDYYDVTKM 339 >SP1632 [T] COG0642 Signal transduction histidine kinase Length = 324 Score = 129 bits (325), Expect = 6e-30, Method: Compositional matrix adjust. Identities = 75/252 (29%), Positives = 143/252 (56%), Gaps = 14/252 (5%) Query: 80 KEANSPFERMVQEAVNA-QTERYRRESSANFQLLEQEKDDLLS----WIHEVKTPLTAMQ 134 +EA SP E + E + A + E Y++ S A E++ DLL W+H++KTP+ A Q Sbjct: 78 REAKSPLEIALAEKLEAREMELYQQRSKA-----ERKLTDLLDYYTLWVHQIKTPIAASQ 132 Query: 135 LMIERLTDETLKPKLMYEWLRVHHLLDQQLHQKRIPFMRNDLYIEMIRLEPVLNKEIQAL 194 L++ + D LK +L E ++ + L R+ +DL ++ +++E ++ + I+ Sbjct: 133 LLVAEVVDRQLKQQLEQEIFKIDSYTNLVLQYLRLESFHDDLVLKQVQIEDLVKEIIRKY 192 Query: 195 KSWCIPKGIGFDVDLAVEAVLTDGKWLGFILRQLLTNAVKYSENSDILIQSREKAGHTVL 254 + I KG+ ++ + ++TD KWL ++ Q+++N++KY++ + I ++ L Sbjct: 193 ALFFIQKGLNVNLHDLDKEIVTDKKWLLVVIEQIISNSLKYTKEGGLEIYMDDQE----L 248 Query: 255 IIEDHGRGIDPKDLPRIYDKGFTSTTGRQEGAATGMGLYLTKQVAEPLQIGIHADSMLGE 314 I+D G GI D+ R++++GF+ GR ++G+GLYL+K+++E L I +S +G+ Sbjct: 249 CIKDTGIGIKNSDVLRVFERGFSGYNGRLTQQSSGLGLYLSKKISEELGHQIRIESEVGK 308 Query: 315 GTTFTLTFPRKN 326 GTT + F + N Sbjct: 309 GTTVRIQFAQVN 320 >CAC0372 [T] COG0642 Signal transduction histidine kinase Length = 334 Score = 127 bits (318), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 101/346 (29%), Positives = 177/346 (51%), Gaps = 38/346 (10%) Query: 1 MIRKYIKEKLSWXXXXXXXXXXXXXVAYVDSSIPFLSVLYIVVLNIMVCTTFVFLRYHKE 60 +I+ ++KEKLS+ YV +I V+Y + + + + F+ + K Sbjct: 5 IIKNFMKEKLSYSISFFLSSGIILFYFYVSKNIT--DVVYPIFMVVSIYIIFIVTEWFKY 62 Query: 61 TRFYQRM--GAWDEVYDLSTFKEANSPFERMVQEAVNAQTERYRRESSANFQLLEQEKDD 118 RF + G E YDL E+ +P +RM QEA+ + Y +A ++ + D Sbjct: 63 YRFNANLTRGIEREYYDL----ESVTPEQRMAQEAILKIHKNY----AAKISEIDYKNSD 114 Query: 119 L----LSWIHEVKTPLTAMQLMIER----LTDETLKPKLMYEWLRVHHLLDQQLHQKRIP 170 + WIH +KTP++ + L+++R L+ + +K + E ++ + +DQ L+ R+ Sbjct: 115 IKYFISQWIHNMKTPVSVIDLIVQREKEKLSGDVIK-NIEEENYKIKNGMDQVLNIIRLD 173 Query: 171 FMRNDLYIEMIRLEPVLNKEIQALKSWCI-----PKGIGFDVDLAVEAVLTDGKWLGFIL 225 D E++ + ++ + I K+ I PK + F VD A+ VLTD KW FI+ Sbjct: 174 EFSRDYEPEIVDIVELVKRTINLKKNQFIYGNAFPK-VEFHVDKAL--VLTDKKWSTFII 230 Query: 226 RQLLTNAVKYSENSDI------LIQSREKAGHTVLIIEDHGRGIDPKDLPRIYDKGFTST 279 Q+++N++KYS+ + ++QS K T L+I+D+G GI DL RI++ FT Sbjct: 231 DQIISNSIKYSKKEEKGYVYFNIVQSNNK---THLVIKDNGVGIPKYDLKRIFEPFFTGE 287 Query: 280 TGRQEGAATGMGLYLTKQVAEPLQIGIHADSMLGEGTTFTLTFPRK 325 GR +TG+GLY+ K++A+ L I +S + +GT +T+ K Sbjct: 288 NGRNFENSTGIGLYICKKIADNLGHEISVESEINKGTIVKITYMAK 333 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.138 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,964,677 Number of extensions: 936277 Number of successful extensions: 2687 Number of sequences better than 1.0e-05: 127 Number of HSP's gapped: 2621 Number of HSP's successfully gapped: 127 Length of query: 334 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 227 Effective length of database: 26,037,693 Effective search space: 5910556311 Effective search space used: 5910556311 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 116 (49.3 bits)