BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0567.0016.p (558 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SP1796 [G] COG1653 ABC-type sugar transport system, periplasmic ... 97 9e-20 BS_lplA [G] COG1653 ABC-type sugar transport system, periplasmic... 72 2e-12 BH0482 [G] COG1653 ABC-type sugar transport system, periplasmic ... 70 1e-11 BS_ytcQ [G] COG1653 ABC-type sugar transport system, periplasmic... 61 6e-09 >SP1796 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 538 Score = 96.7 bits (239), Expect = 9e-20, Method: Compositional matrix adjust. Identities = 145/560 (25%), Positives = 246/560 (43%), Gaps = 73/560 (13%) Query: 35 RISIMLAATLFSSILAACGSSTDKSGSAALPSQEPGQYGDTGTLTLPLVDKPTT--ITYM 92 + +++L A+L ++LAACGS +A+ P Y G +T PL +K T +T Sbjct: 8 KSAVLLTASL--AVLAACGSKN----TASSPD-----YKLEG-VTFPLQEKKTLKFMTAS 55 Query: 93 LPSNAKDLGPGKLVVQELEKRTGIKVDFQTYSPQTYQDKLKVIVASGKLPDIFT--GLKP 150 P + KD KL++Q LEK TG+ +D+ Y + +K + ++SG LPD G Sbjct: 56 SPLSPKDPNE-KLILQRLEKETGVHIDWTNYQ-SDFAEKRNLDISSGDLPDAIHNDGASD 113 Query: 151 AELKKIGKQNGVVAINKYADQ-LPNFKKLYMEENDW-VLKSFGDEAGDIYTWP-IYNLN- 206 +L K+ ++ + D+ +PN KK+ E+ ++ L + D G IY++P I L Sbjct: 114 VDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPD--GHIYSFPWIEELGD 171 Query: 207 -----RKVNHGFMFRKDIFDELGIKEWTNTDEFYEALKKVKAAYP------DSYPYASKS 255 VN KD +LG++ TD+ + L+ K P D P++ S Sbjct: 172 GKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNGNGEADEIPFSFIS 231 Query: 256 LANIFRDWAF---GWGLGNTDQYPAYYDEKDGTWKYAAVQQAHKDMLDFMKKLYNEKLLD 312 N D+ F +G+G+ D + + DG + A +K+ + F+++L + L+D Sbjct: 232 -GNGNEDFKFLFAAFGIGDNDDHLVVGN--DGKVDFTADNDNYKEGVKFIRQLQEKGLID 288 Query: 313 PEFLTDTQDSWTTKMTTDKSFVTFDWIGRLDLFYNQIKEQNPDYDLR--YANPVGPTGNV 370 E +S+ K K V F W N + N YD+ A P G Sbjct: 289 KEAFEHDWNSYIAKGHDQKFGVYFTWDK------NNVTGSNESYDVLPVLAGPSGQKHVA 342 Query: 371 RT--LPEVSDFSVAVAKNKNTEASLKLLDYLTSPSGSELMTIGVEGVN-----FEWGEDG 423 RT + D V + NKN E + K +D +P S G G + FE + Sbjct: 343 RTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQQNIFELDQ-- 400 Query: 424 FPVYPELKDLPLVDITVLEDRYAMWLEGAYLRPDHRSIYYRFSEKEQEAQDK--IVKEDR 481 LK LPL E R + G D S Y + + +A+ + ++KE Sbjct: 401 --ASNSLKHLPLNGTAPAELRQKTEVGGPLAILD--SYYGKVTTMPDDAKWRLDLIKEYY 456 Query: 482 FEPLDPILNF-----TDEETSKIAELQTSLQKSAEEFNAKYILDAGYGEAQWADFQTKLS 536 + + N+ T E+ KIA ++ + A++I++ G + +W D++ +L Sbjct: 457 VPYMSNVNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVN-GNIDTEWDDYKKELE 515 Query: 537 RSGVEELMSIYNEAQKRYDE 556 + G+ + ++I QK YD+ Sbjct: 516 KYGLSDYLAI---KQKYYDQ 532 >BS_lplA [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 502 Score = 72.0 bits (175), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 134/555 (24%), Positives = 222/555 (40%), Gaps = 78/555 (14%) Query: 28 RLMMLKKRISIMLAATLFSSILAACGSSTDKSGSAALPSQEPGQYGDTGTLTLPLVDKPT 87 ++ M KK +++ LAA + +A C S ++ S SA+ DT + PL+ Sbjct: 2 KIRMRKKWMALPLAAMM----IAGC-SHSETSNSASGSK-------DTIKIMAPLLS--- 46 Query: 88 TITYMLPSNAKDLGPGKLVVQELEKRTGIKVDFQTYSPQTYQDKLKVIVASGKLPD-IFT 146 P + D P ++ LEK TG ++ +Y DK +++ASG++P I Sbjct: 47 ------PESPSDKSPS---LKALEKYTGKEIKVTWVPDSSYNDKFNIVMASGEMPHAIVI 97 Query: 147 GLKPAELKKIGKQNGVVAINKYADQLPNFKKLYMEENDWVLKSFGDEAGDIYTWPIYNLN 206 K A K K ++ Y L ++K L + ++ +LK+ G++Y IY Sbjct: 98 KDKSAGFIKSVKAGAFWELSPY---LKDYKNLS-QADEKILKN-SSVNGEVYG--IYRTR 150 Query: 207 RKVNHGFMFRKDIFDELGIKEWTNTDEFYEALKKVKAAYPDSYPYASKSLANIFRDWAFG 266 + + R D +G+ D+FYE LK K PD + + W Sbjct: 151 DLIRACMIIRTDWLKNVGLDMPETLDDFYEVLKAFKEKDPDGNG-KDDTYGMVVPKWM-- 207 Query: 267 WGLGNTDQY---------PAYYDEKDGTWKYAAVQQAHKDMLDFMKKLYNEKLLDPEFLT 317 GLGN + P Y ++G + + D L F KKLY+E L++ +F Sbjct: 208 -GLGNGSPWDVLQIWFGAPNRYGVENGKLIPDFTTKEYMDALTFFKKLYDEGLINKDFAV 266 Query: 318 DTQDSWTTKMTTDKSFVTFDWIGRLDLFYNQIK---EQNPDYDLRYANPVGPTGNVRTLP 374 W + K+ V D R + ++ E N D + GP G RT P Sbjct: 267 MDSAKWNDPVVKGKAGVIVDTGSRASQIQSAMEEADESNKDIIDIVGSLEGPNGK-RTFP 325 Query: 375 EVSDFS-------VAVAKNKNTEASLKLLDYLTSPSGSELMTIGVEGVNFEWGEDGFPVY 427 S +S +V K + L LD + L GV+G N+E +DG Sbjct: 326 -TSGYSGMITIPKSSVKTEKELKEVLSFLDKMNDKEAQILTNNGVKGRNYEL-KDGVFTS 383 Query: 428 PELKDLPLVDITVLEDRYAMWL-EGAYLRPDHRSIYYRFSEKEQEAQDKIVKEDRFEPLD 486 E + L+ +++M + + Y D ++ + Q +D I + ++ + Sbjct: 384 LEKNNKSLLYEHEGLAQFSMSIPKSEYYIEDQKTKLF------QHRKDIITEGEKIAVFN 437 Query: 487 PILNF-TDEETSKIAELQTSLQKSAEEFNAKYILDAGYGEAQ--WADFQTKLSRSGVEEL 543 P + +D T K A+L + + +F I + G+ +A W +SG EL Sbjct: 438 PAESLVSDVYTQKGAQLDNIILDARTQFIIGEIDEKGFDDAVELW-------KKSGGNEL 490 Query: 544 MSIYNEAQKRYDESK 558 M N K Y SK Sbjct: 491 MKDLN---KLYQSSK 502 >BH0482 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 512 Score = 70.1 bits (170), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 112/474 (23%), Positives = 194/474 (40%), Gaps = 51/474 (10%) Query: 102 PGKLVVQELEKRTGIKVDFQTYSPQTYQDKLKVIVASGKLPDIFT--GLKPAELKKIGKQ 159 P V++E+E+ T +D +D++ +ASG LP + T + + + + Sbjct: 66 PKDTVIKEIEELTNTNLDLIWVPDAVKEDRVNAALASGNLPKVITLQDFRTSSVLNAFRS 125 Query: 160 NGVVAINKYADQLPNFKKLYMEENDWVLKSFGDEAGDIYTWPIYNLNRKVNHGFMFRKDI 219 I Y D+ PN ++ N+ +L++ + G IY IY G + R+D Sbjct: 126 GQFWEIGPYLDEFPNLSEM----NETILENVSVD-GKIYG--IYRERPLSRQGVVIRQDW 178 Query: 220 FDELGIKEWTNTDEFYEALKKVKAAYPDSYPYASKSLANIFRDWAFGW--GLGNTDQYPA 277 + LG++ TN +E YE + P+ D FG L + P Sbjct: 179 LENLGLEMPTNLEELYEVARAFTYDDPNGSGADDTVGFTDRNDLIFGAFKTLASYHGTPN 238 Query: 278 YYD-EKDGTWKYAAVQQAHKDMLDFMKKLYNEKLLDPEFLTDTQDSWTTKMTTDKSFVTF 336 + + +G + + + +++MK LY+E L++ EF +++ T ++ + Sbjct: 239 EWGFDDEGKLMPEFMTDEYVETMNYMKSLYDEGLINTEFPVTSKEQQQELFMTGRAGIYV 298 Query: 337 DWIGRLDLFYNQIKEQNPDYDLRYANPV-GPTGNVRTLPEVSDFSV-AVAKNK-NTEASL 393 + ++ K+ NP+ ++ N + GP G R + + A+ K+ TEA L Sbjct: 299 GNMVDARNLRDKAKDINPEMEVAITNRIAGPDGEDRVWAQGGHNGIFAIPKSSVETEAEL 358 Query: 394 K----LLDYLTSPSGSELMTIGVEGVNFEW--GEDGFPVYPELKDL-----PLVDITVLE 442 K D + LM G+E V++E GE + L D PLV + E Sbjct: 359 KRILAFFDRIAEEDVYNLMAYGIEDVHYETIDGEYFERIEENLDDWAAEVQPLVSLVARE 418 Query: 443 DRYAMWLEGAYLRPDHRSIYYRFSEKEQEAQDKIVKEDRFEPLDPILNFTDEETSKIAEL 502 Y AY P Y E+ E ++I+ + EPL F+ T E Sbjct: 419 MNYLK----AYGDP-----LYEEYERLTEDNNEIIVTNPAEPL-----FSPTYT----ER 460 Query: 503 QTSLQKSAEEFNAKYILDAGYGEAQWADFQTKLSR---SGVEELMSIYNEAQKR 553 T LQK ++ KYIL GE F+ ++ R +G E ++ NE +R Sbjct: 461 GTELQKIIDDATYKYIL----GELDEDGFEAEVERWLTNGGESIIEELNEDYER 510 >BS_ytcQ [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 440 Score = 60.8 bits (146), Expect = 6e-09, Method: Compositional matrix adjust. Identities = 82/334 (24%), Positives = 135/334 (40%), Gaps = 24/334 (7%) Query: 102 PGKLVVQELEKRTGIKVDFQTYSPQTYQDKLKVIVASGKLPDIFT--GLKPAELKKIGKQ 159 P ++E+EK T ++D +D+L +A+G LP I T +K + + + Sbjct: 46 PKDRAIKEIEKLTNTELDITWVPDAVKEDRLNAALAAGNLPQIVTIQDIKNSSVMNAFRS 105 Query: 160 NGVVAINKYADQLPNFKKLYMEENDWVLKSFGDEAGDIYTWPIYNLNRKVNHGFMFRKDI 219 I Y PN K+ N + K+ + G +Y IY G + RKD Sbjct: 106 GMFWEIGDYIKDYPNLNKM----NKLINKNVTID-GKLYG--IYRERPLSRQGIVIRKDW 158 Query: 220 FDELGIKEWTNTDEFYEALKK-VKAAYPDSYPYASKSLANIFRDWAFGW--GLGNTDQYP 276 D L +K DE YE K + + LA+ D +G +G+ + P Sbjct: 159 LDNLNLKTPKTLDELYEVAKAFTEDDPDKDGKDDTFGLAD-RNDLIYGAFKTIGSYEGMP 217 Query: 277 AYYDEKDGTWKYAAVQQAHKDMLDFMKKLYNEKLLDPEFLTDTQDSWTTKMTTDKSFVTF 336 + E G + + Q +KD +++MKKL + ++ +F ++ + K+ + Sbjct: 218 TDWKESGGKFTPDFMTQEYKDTMNYMKKLRDNGYMNKDFPVTSKTQQQELFSQGKAGI-- 275 Query: 337 DWIGRL-DLFYNQIKEQNPDYDLRYANPV-GPTGNVRTLPEVSDFSV-AVAKNK-NTEAS 392 +IG + D + + L N + GP G R V A K TEA Sbjct: 276 -YIGNMVDAVNLRDHASDKSMKLEIINRIKGPDGKERVWASGGHNGVFAFPKTSVKTEAE 334 Query: 393 LK----LLDYLTSPSGSELMTIGVEGVNFEWGED 422 LK D + LMT G++GV++ GED Sbjct: 335 LKRILAFFDRIAEEDVYSLMTYGIDGVHYNKGED 368 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.134 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 44,016,974 Number of extensions: 1996435 Number of successful extensions: 7477 Number of sequences better than 1.0e-05: 6 Number of HSP's gapped: 7474 Number of HSP's successfully gapped: 6 Length of query: 558 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 446 Effective length of database: 25,461,023 Effective search space: 11355616258 Effective search space used: 11355616258 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 118 (50.1 bits)