BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0567.0012.p (335 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp1011_1 [ER] COG1063 Threonine dehydrogenase and related Zn-de... 166 5e-41 STM3261 [ER] COG1063 Threonine dehydrogenase and related Zn-depe... 59 7e-09 ZgatD [ER] COG1063 Threonine dehydrogenase and related Zn-depend... 56 1e-07 gatD [ER] COG1063 Threonine dehydrogenase and related Zn-depende... 56 1e-07 ZgatD [ER] COG1063 Threonine dehydrogenase and related Zn-depend... 56 1e-07 >RSp1011_1 [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Length = 397 Score = 166 bits (420), Expect = 5e-41, Method: Compositional matrix adjust. Identities = 103/327 (31%), Positives = 160/327 (48%), Gaps = 9/327 (2%) Query: 9 GKVAILQAAIPELNK-RHVQVRTEYSGISPGTELSSIKRSGLSPVSLGYSAVGIVEQTGS 67 G+ ++L A + K + V + G+S T LS+++ P+ LGY VG V G+ Sbjct: 49 GRASLLAKARQQPEKVKMVLNKIATDGLS--TTLSAVRAKLNQPIPLGYCNVGTVHAVGA 106 Query: 68 EVRDIHPGDRVACYGVPYVRHAEVISVPTNLVTKVPHHVNPEEAAFTGLGAIAIHALRTA 127 V GDRV G HA+V+ VP NL ++P V+ E A FT + AI + +R A Sbjct: 107 GVEGFKVGDRVVSNG----SHADVVRVPRNLCARIPDEVDDESAVFTVVAAIGLQGIRLA 162 Query: 128 DVRFXXXXXXXXXXXXXXXXAQIASAAACHTAAYDLSEDRVQLLQGQMGITSGFSSEEEV 187 + Q+ A C A D ++++L T E+ Sbjct: 163 EPTLGEAFVVTGVGLIGLLAVQLLRAHGCRVLAIDFDSEKLKLAAQFGAETCNPGLREDP 222 Query: 188 EHFVMNETGGAGFDSILLCAGGPGEVLINKSLEWLRDLGKIVIVGDLSMEFSRDLMFRKE 247 + + G G D +++ A + ++ + R G+IV+VG +E +R + KE Sbjct: 223 VAAGLAFSRGRGVDGVIITASTASSEPVTQAAQMCRKRGRIVLVGVTGLELNRADFYEKE 282 Query: 248 AQILISRAGGPGRYDMRYEEDNQDYPIGFVRWTEGRNMDEYVRLLAEKRITVSPAITHRF 307 +S + GPGRYD YEE +DYP+GFVRWTE RN + + +LA++R+ V P ITHR Sbjct: 283 LSFQVSCSYGPGRYDPTYEEQGRDYPLGFVRWTEQRNFEAVLDMLADRRLDVRPLITHRI 342 Query: 308 ALEEAPAAYGIYQSGPAQGALATLIKY 334 +EAP AY + S + AL +++Y Sbjct: 343 PFDEAPRAYDLLSSD--KSALGIVLEY 367 >STM3261 [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Length = 347 Score = 59.3 bits (142), Expect = 7e-09, Method: Compositional matrix adjust. Identities = 86/362 (23%), Positives = 139/362 (38%), Gaps = 59/362 (16%) Query: 3 IVAAKEGKVAILQAAIPELN-KRHVQVRTEYSGISPGTELSSIKRSGLS--PVSLGYSAV 59 +V EG V + + +P+L + V V+ SG+ G+++ I G P++LG+ Sbjct: 4 VVIHAEGDVRVEERPLPQLQAEDDVLVKVVSSGLC-GSDIPRIFAQGAHYYPITLGHEFS 62 Query: 60 GIVEQTGSEVRDIHPGDRVAC------YGVPYVR--------------------HAEVIS 93 G VE G+ V D+ PGD VAC + P +AE + Sbjct: 63 GYVESYGTGVTDMQPGDAVACVPLLPCFHCPQCERGYFSLCKQYQFVGSRSEGGNAEYVV 122 Query: 94 VPTNLVTKVPHHVNPEEAAFTGLGAIAIHALRTADVRFXXXXXXXXXXXXXXXXAQIA-S 152 V + ++P + E+ AF + +HA A Q A Sbjct: 123 VKRANLFRLPSDMPIEDGAFIEPITVGLHAFHLAQGCEGKNVIIVGAGTIGLLALQCARE 182 Query: 153 AAACHTAAYDLSEDRVQLLQGQMGITSGFSSEEEVEHFVMNETGGAGFDSILL-CAGGPG 211 A A D++ +++L + +G T +S E + FD ++L AG P Sbjct: 183 LGARSVTAIDINPQKLELAKA-LGATHTCNSREMTADDIQTALSDIQFDQLVLETAGTPQ 241 Query: 212 EVLINKSLEWLRDLGKIVIVGDLSMEFSRD-----LMFRKEAQILISRAGGPGRYDMRYE 266 V + + R ++ +VG L + + L+ RKE +L S M Y Sbjct: 242 TVSLAIDIAGPR--AQLALVGTLHHDLTLTTRTFGLILRKELTLLGSW--------MNY- 290 Query: 267 EDNQDYPIGFVRWTEGRNMDEYVRLLAEKRITVSPAITHRFALEEAPAAYGIYQSGPAQG 326 + +P G + RLLAEKR+ ++P I HR E A P QG Sbjct: 291 --SAPWP--------GEEWETAARLLAEKRLQLTPLIAHRGDAESFAEAVKALNGAPMQG 340 Query: 327 AL 328 + Sbjct: 341 KI 342 >ZgatD [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Length = 346 Score = 55.8 bits (133), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 81/344 (23%), Positives = 140/344 (40%), Gaps = 59/344 (17%) Query: 3 IVAAKEGKVAILQAAIPEL-NKRHVQVRTEYSGISPGTELSSIKRSGLS--PVSLGYSAV 59 +V +G V + ++ IPE+ ++ V+V+ SG+ G++L I ++G P++LG+ Sbjct: 4 VVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLC-GSDLPRIFKNGAHYYPITLGHEFS 62 Query: 60 GIVEQTGSEVRDIHPGDRVAC------YGVPYVRH--------------------AEVIS 93 G ++ GS V D+HPGD VAC + P AE I Sbjct: 63 GYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQCAKYDFIGSRRDGGFAEYIV 122 Query: 94 VPTNLVTKVPHHVNPEEAAFTGLGAIAIHALRTADVRFXXXXXXXXXXXXXXXXAQIASA 153 V V +P + E+ AF + +HA A Q A A Sbjct: 123 VKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCENKNVIIIGAGTIGLLAIQCAVA 182 Query: 154 -AACHTAAYDLSEDRVQLLQGQMGITSGFSSEEEVEHFVMNETGGAGFDSILL-CAGGPG 211 A A D+S +++ L + G F+S E + + F+ ++L AG P Sbjct: 183 LGAKSVTAIDISSEKLALAK-SFGAMQTFNSSEMSAPQMQSVLRELRFNQLILETAGVPQ 241 Query: 212 EVLINKSLEWLRDLGKIVIVGDLSMEF-----SRDLMFRKEAQILISRAGGPGRYDMRYE 266 V + ++E ++ +VG L + + + RKE ++ S M Y Sbjct: 242 TVEL--AVEIAGPHAQLALVGTLHQDLHLTSATFGKILRKELTVIGSW--------MNY- 290 Query: 267 EDNQDYPIGFVRWTEGRNMDEYVRLLAEKRITVSPAITHRFALE 310 + +P G+ + RLL E+++++ P I HR + E Sbjct: 291 --SSPWP--------GQEWETASRLLTERKLSLEPLIAHRGSFE 324 >gatD [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Length = 346 Score = 55.8 bits (133), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 81/344 (23%), Positives = 140/344 (40%), Gaps = 59/344 (17%) Query: 3 IVAAKEGKVAILQAAIPEL-NKRHVQVRTEYSGISPGTELSSIKRSGLS--PVSLGYSAV 59 +V +G V + ++ IPE+ ++ V+V+ SG+ G++L I ++G P++LG+ Sbjct: 4 VVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLC-GSDLPRIFKNGAHYYPITLGHEFS 62 Query: 60 GIVEQTGSEVRDIHPGDRVAC------YGVPYVRH--------------------AEVIS 93 G ++ GS V D+HPGD VAC + P AE I Sbjct: 63 GYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQCAKYDFIGSRRDGGFAEYIV 122 Query: 94 VPTNLVTKVPHHVNPEEAAFTGLGAIAIHALRTADVRFXXXXXXXXXXXXXXXXAQIASA 153 V V +P + E+ AF + +HA A Q A A Sbjct: 123 VKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCENKNVIIIGAGTIGLLAIQCAVA 182 Query: 154 -AACHTAAYDLSEDRVQLLQGQMGITSGFSSEEEVEHFVMNETGGAGFDSILL-CAGGPG 211 A A D+S +++ L + G F+S E + + F+ ++L AG P Sbjct: 183 LGAKSVTAIDISSEKLALAK-SFGAMQTFNSSEMSAPQMQSVLRELRFNQLILETAGVPQ 241 Query: 212 EVLINKSLEWLRDLGKIVIVGDLSMEF-----SRDLMFRKEAQILISRAGGPGRYDMRYE 266 V + ++E ++ +VG L + + + RKE ++ S M Y Sbjct: 242 TVEL--AVEIAGPHAQLALVGTLHQDLHLTSATFGKILRKELTVIGSW--------MNY- 290 Query: 267 EDNQDYPIGFVRWTEGRNMDEYVRLLAEKRITVSPAITHRFALE 310 + +P G+ + RLL E+++++ P I HR + E Sbjct: 291 --SSPWP--------GQEWETASRLLTERKLSLEPLIAHRGSFE 324 Score = 55.8 bits (133), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 81/344 (23%), Positives = 140/344 (40%), Gaps = 59/344 (17%) Query: 3 IVAAKEGKVAILQAAIPEL-NKRHVQVRTEYSGISPGTELSSIKRSGLS--PVSLGYSAV 59 +V +G V + ++ IPE+ ++ V+V+ SG+ G++L I ++G P++LG+ Sbjct: 4 VVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLC-GSDLPRIFKNGAHYYPITLGHEFS 62 Query: 60 GIVEQTGSEVRDIHPGDRVAC------YGVPYVRH--------------------AEVIS 93 G ++ GS V D+HPGD VAC + P AE I Sbjct: 63 GYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQCAKYDFIGSRRDGGFAEYIV 122 Query: 94 VPTNLVTKVPHHVNPEEAAFTGLGAIAIHALRTADVRFXXXXXXXXXXXXXXXXAQIASA 153 V V +P + E+ AF + +HA A Q A A Sbjct: 123 VKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCENKNVIIIGAGTIGLLAIQCAVA 182 Query: 154 -AACHTAAYDLSEDRVQLLQGQMGITSGFSSEEEVEHFVMNETGGAGFDSILL-CAGGPG 211 A A D+S +++ L + G F+S E + + F+ ++L AG P Sbjct: 183 LGAKSVTAIDISSEKLALAK-SFGAMQTFNSSEMSAPQMQSVLRELRFNQLILETAGVPQ 241 Query: 212 EVLINKSLEWLRDLGKIVIVGDLSMEF-----SRDLMFRKEAQILISRAGGPGRYDMRYE 266 V + ++E ++ +VG L + + + RKE ++ S M Y Sbjct: 242 TVEL--AVEIAGPHAQLALVGTLHQDLHLTSATFGKILRKELTVIGSW--------MNY- 290 Query: 267 EDNQDYPIGFVRWTEGRNMDEYVRLLAEKRITVSPAITHRFALE 310 + +P G+ + RLL E+++++ P I HR + E Sbjct: 291 --SSPWP--------GQEWETASRLLTERKLSLEPLIAHRGSFE 324 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.135 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,779,233 Number of extensions: 1088740 Number of successful extensions: 11607 Number of sequences better than 1.0e-05: 8 Number of HSP's gapped: 11636 Number of HSP's successfully gapped: 8 Length of query: 335 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 228 Effective length of database: 26,037,693 Effective search space: 5936594004 Effective search space used: 5936594004 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)