BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0566.0034.p (228 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Ta1199 [H] COG0043 3-polyprenyl-4-hydroxybenzoate decarboxylase ... 91 1e-18 AF0209 [H] COG0043 3-polyprenyl-4-hydroxybenzoate decarboxylase ... 87 2e-17 PAE1844 [H] COG0043 3-polyprenyl-4-hydroxybenzoate decarboxylase... 83 3e-16 STM3978 [H] COG0043 3-polyprenyl-4-hydroxybenzoate decarboxylase... 77 2e-14 PA5237 [H] COG0043 3-polyprenyl-4-hydroxybenzoate decarboxylase ... 74 2e-13 >Ta1199 [H] COG0043 3-polyprenyl-4-hydroxybenzoate decarboxylase and related decarboxylases Length = 480 Score = 90.9 bits (224), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 61/196 (31%), Positives = 101/196 (51%), Gaps = 20/196 (10%) Query: 1 MEECIVDLEKHGHLVRIQEEVDPNLEMAAIHLKVYEAG-GPALLFEKVKGSRYRAVSNLF 59 + E + L + L+ + ++VDP+LE+ I + G G + F +VKGS AV NLF Sbjct: 5 LHEYLDFLARKNDLITVNDQVDPDLELTYILSEEERIGRGRTIQFNRVKGSEVPAVGNLF 64 Query: 60 GTIERSKFIF-RDTWESSQDVIALRSDPMKALKQPFKYIGTGLAARK---ALPLKKGNGI 115 T E+ K + D ++ + ++ + P + +IG G+ + L K + Sbjct: 65 STYEKMKTVLGDDPYQIGRRIVEIAQPPGDSES----FIGKGIEMMRELGGLRPKIAGSL 120 Query: 116 PAGFEEIQISDL---PQIKHWPGDGGAFVTLPQVYSEDPDKPGIMNSNLGMYRIQLSGNE 172 P+ ++E+ DL P K WP DGG F+TLP V ++DP+ N+GMYR+Q+ +E Sbjct: 121 PSNYDELDRVDLFRYPICKTWPQDGGKFITLPLVITKDPETG---TRNMGMYRMQVYDSE 177 Query: 173 YEINKEVGVHYQIHRG 188 G+H+ IH+G Sbjct: 178 -----TTGMHWHIHKG 188 >AF0209 [H] COG0043 3-polyprenyl-4-hydroxybenzoate decarboxylase and related decarboxylases Length = 481 Score = 87.0 bits (214), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 67/195 (34%), Positives = 91/195 (46%), Gaps = 22/195 (11%) Query: 1 MEECIVDLEKHGHLVRIQEEVDPNLEMAAIHLKVYEAGGPALLFEKVKGSRYRAVSNLFG 60 + E I LE G L R++ EV P LEM+ + + +AGG ALLFE+ KG N FG Sbjct: 6 LREFIGRLEDKGELARVKHEVSPILEMSEVADRTVKAGGKALLFERPKGYDIPVFMNAFG 65 Query: 61 TIERSKFI-----FRDTWESSQDVIALR-SDPMKALKQPFKYIGTGLAA--RKALPLKKG 112 T R K + E + R S M ALK G G+ +P K G Sbjct: 66 TERRMKLALEVERLEEIGERLLSALEFRPSSFMDALK------GVGMLKDFMSFIPKKTG 119 Query: 113 NGIPAGFEEIQISDLPQIKHWPGDGGAFVTLPQVYSEDPDKPGIMNSNLGMYRIQLSGNE 172 + P +K WP D G F+T P V ++DP+ G MN+ GMYR+Q+ Sbjct: 120 KAPCKEVVAESLDKFPILKCWPKDAGRFITFPVVITKDPET-GEMNA--GMYRMQVFD-- 174 Query: 173 YEINKEVGVHYQIHR 187 K G+H+QIH+ Sbjct: 175 ---GKTTGMHWQIHK 186 >PAE1844 [H] COG0043 3-polyprenyl-4-hydroxybenzoate decarboxylase and related decarboxylases Length = 475 Score = 83.2 bits (204), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 61/193 (31%), Positives = 100/193 (51%), Gaps = 18/193 (9%) Query: 1 MEECIVDLEKHGHLVRIQEEVDPNLEMAAIHLKVYEAGGPALLFEKVKG-SRYRAVSNLF 59 + E + LE+ G L R+ E + P LE+ + +V A GPA+LFE V+G ++R V NLF Sbjct: 5 LREFLKALEERGWLKRVSEPLSPELEIPEVLRQVMYARGPAVLFESVRGFPKWRVVGNLF 64 Query: 60 GTIERSKFIFRDTWESSQDVI-ALRSDPMKALKQPFK----YIGTGLAARKALPLKKGNG 114 G+++R + + E+ + ++ + S P L F+ G A +A+ +G Sbjct: 65 GSLDRIRLALGELEEAGRRLLEPMASPPPLTLADKFRAAVELFELGRYAPRAV---RGGP 121 Query: 115 IPAGFEEIQISDLPQIKHWPGDGGAFVTLPQVYSEDPDKPGIMNSNLGMYRIQLSGNEYE 174 + EE + +P K WP D G ++T + + D G+ NLG+YR+Q+ NE Sbjct: 122 VKEVVEEPNLLSIPAFKTWPKDAGQYITFGVLITR--DSRGVY--NLGVYRVQIL-NE-- 174 Query: 175 INKEVGVHYQIHR 187 +E VH QIH+ Sbjct: 175 --REAVVHAQIHK 185 >STM3978 [H] COG0043 3-polyprenyl-4-hydroxybenzoate decarboxylase and related decarboxylases Length = 492 Score = 77.0 bits (188), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 66/203 (32%), Positives = 93/203 (45%), Gaps = 28/203 (13%) Query: 1 MEECIVDLEKHGHLVRIQEEVDPNLEMAAIHLKVYEAGGPALLFEKVKGSRYRAVSNLFG 60 + + + LE+ G L RI VDP+LE+ I + AGGPALLFE KG + NLFG Sbjct: 9 LRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLRAGGPALLFENPKGYAMPVLCNLFG 68 Query: 61 TIERSKFIFRDTWESSQDVIALRS--DPMKALKQP-----FKYIGTGLAARKA---LPLK 110 T +R DV ALR + LK+P F+ + L K +P K Sbjct: 69 TPKRVAMGM-----GQDDVSALREVGKLLAFLKEPEPPKGFRDLFDKLPQFKQVLNMPTK 123 Query: 111 KGNGIP-----AGFEEIQISDLPQIKHWPGDGGAFVTLPQVYSEDPDKPGIMNSNLGMYR 165 + G P A +++ ++ LP + WP D +T + P K NLG+YR Sbjct: 124 RLRGAPCQQKIASGDDVDLTRLPVMTCWPDDAAPLITWGLTVTRGPHKE---RQNLGIYR 180 Query: 166 IQLSGNEYEINKEVGVHYQIHRG 188 QL G NK + + + HRG Sbjct: 181 QQLIGK----NKLI-MRWLSHRG 198 >PA5237 [H] COG0043 3-polyprenyl-4-hydroxybenzoate decarboxylase and related decarboxylases Length = 488 Score = 73.9 bits (180), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 64/203 (31%), Positives = 87/203 (42%), Gaps = 28/203 (13%) Query: 1 MEECIVDLEKHGHLVRIQEEVDPNLEMAAIHLKVYEAGGPALLFEKVKGSRYRAVSNLFG 60 + + I LE+ G L RIQ + P LEM + + A GPALLFEK G + NLFG Sbjct: 6 LRDFIAQLEQRGALKRIQVPISPVLEMTEVCDRTLRAKGPALLFEKPTGFDMPVLGNLFG 65 Query: 61 TIERSKFIFRDTWESSQDVIALRS-----------DPMKALKQPFK----YIGTGLAARK 105 T ER ++DV ALR +P K LK + Y A K Sbjct: 66 TPERVALGM-----GAEDVGALREIGKLLAQLKEPEPPKGLKDAWAKLPMYRKVLSMAPK 120 Query: 106 ALPLKKGNGIPAGFEEIQISDLPQIKHWPGDGGAFVTLPQVYSEDPDKPGIMNSNLGMYR 165 L + E++ + LP WPGD G +T + P+K NLG+YR Sbjct: 121 VLKDAPCQEVVEEGEDVDLGRLPVQTCWPGDVGPLITWGLTVTRGPNKE---RQNLGIYR 177 Query: 166 IQLSGNEYEINKEVGVHYQIHRG 188 Q+ G +V + + HRG Sbjct: 178 QQVIG-----RNKVIMRWLSHRG 195 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.140 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 16,774,722 Number of extensions: 693057 Number of successful extensions: 922 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 909 Number of HSP's successfully gapped: 10 Length of query: 228 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 125 Effective length of database: 26,499,029 Effective search space: 3312378625 Effective search space used: 3312378625 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 114 (48.5 bits)