BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0566.0029.p (339 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value L121175 [IQR] COG1028 Dehydrogenases with different specificitie... 280 3e-75 CC0398 [IQR] COG1028 Dehydrogenases with different specificities... 229 5e-60 VNG0479G [IQR] COG1028 Dehydrogenases with different specificiti... 142 7e-34 ML0315 [IQR] COG1028 Dehydrogenases with different specificities... 122 7e-28 Rv0068 [IQR] COG1028 Dehydrogenases with different specificities... 122 1e-27 >L121175 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 339 Score = 280 bits (716), Expect = 3e-75, Method: Compositional matrix adjust. Identities = 149/340 (43%), Positives = 213/340 (62%), Gaps = 13/340 (3%) Query: 9 LTVQAPLAS-GFDARTTAAEVIGGLDLTGKVAIVTGGYSGIGLETSRVLAEAGATVIVPA 67 ++ Q P+A+ F+ +TTA +V+ ++L+GK I+TGGYSGIGLE ++ L+ AGA +I+PA Sbjct: 1 MSNQEPIANYKFNYKTTANKVMKNINLSGKTIIITGGYSGIGLEMTKQLSRAGAKIIIPA 60 Query: 68 RTPAKAKAAVSGIPRLELETLDLMDPASIDSFARRFIASGRPLHFLINSAGIMAAPLARD 127 R AK +S I +++ LDL +P SI FA F+ + L LINSAG+M PL RD Sbjct: 61 RRMEVAKKNLSKIKNIDILPLDLTNPESIHQFAESFLQNNSKLDILINSAGLMYLPLKRD 120 Query: 128 ARGYESQFATNHLGHFQLTARLWPALRQADQARVISVSSRAHRLGGVDLSDPNFERRE-Y 186 +RG E FATNHLGH+QLT L PAL+ A +RVI++SSRA ++ + L D NFE+ E Y Sbjct: 121 SRGIEYHFATNHLGHYQLTLELLPALKNAKISRVINLSSRAQQMTPL-LEDWNFEKIENY 179 Query: 187 DKWKSYAQSKSANVLFAVALDEKAKVYGVRAFAVHPGLIPSSGIGR-------FLKTEEV 239 + Y QSK+ANVLF++ LDE K YG+R+FAVHPG+IP + IGR +L+ Sbjct: 180 NPQIGYQQSKTANVLFSIKLDELGKKYGIRSFAVHPGMIPMTNIGRQNSPITPWLRYLAD 239 Query: 240 GVKPVKVQPAQEDEKGKPASNTNSDFMKNTEQGAATSVWCAVSEQLAGLGGVYCEDADIA 299 K + + P + + ++K QGAAT +W + S L G GG+YCED +IA Sbjct: 240 YFKLIHI-PVFFNALKSGFDRSKYRYLKTIAQGAATPLWASTSPDLEGKGGLYCEDLNIA 298 Query: 300 EAVPADSVQG--SGVWPWAIDREAAERLWVLSEQLTGVKF 337 + ++ G+ P AID A+RLW +S+ +TG+ F Sbjct: 299 RLMTSEEANNFSGGLRPHAIDHNDADRLWQISKNITGLDF 338 >CC0398 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 323 Score = 229 bits (584), Expect = 5e-60, Method: Compositional matrix adjust. Identities = 145/332 (43%), Positives = 194/332 (58%), Gaps = 28/332 (8%) Query: 15 LASGFDARTTAAEVIGGLDLTGKVAIVTGGYSGIGLETSRVLAEAGATVIVPARTP---- 70 + S F A++TA EV+ G DL+G+VAIVTG +GIG+ET+R LA AGA VI+ AR P Sbjct: 5 ITSAFGAKSTAREVVAGHDLSGRVAIVTGAATGIGVETARALALAGAEVIIAARKPELGE 64 Query: 71 --AKAKAAVSGIPRLELETLDLMDPASIDSFARRFIASGRPLHFLINSAGIMAAPLARDA 128 A A +G R+ LDL +I F + R + LIN+A +MA+PL R A Sbjct: 65 EVANAINEEAGSKRVSFGMLDLSSLEAIRHFVN--VWGDRRIDILINNAAVMASPLMRTA 122 Query: 129 RGYESQFATNHLGHFQLTARLWPALRQADQA-----RVISVSSRAHRLGGVDLSDPNFER 183 G+E QF TNHLGHF L+ L P L +A R++S+SS HR G+ DPN+ Sbjct: 123 DGFEMQFGTNHLGHFLLSVLLAPNLIAGAKASGKPSRLVSLSSIGHRRSGIHFDDPNYTT 182 Query: 184 REYDKWKSYAQSKSANVLFAVALDEKAKVYGVRAFAVHPGLIPSSGIGRFLKTEEVGVKP 243 R Y+KW++Y Q+K+AN LFAV D++ K GV A AV PG I + + R L EE Sbjct: 183 RPYEKWEAYGQAKTANSLFAVGFDKRFKDQGVHANAVMPGGILTP-LQRHLPIEE----- 236 Query: 244 VKVQPAQEDEKGKPASNTNSDFMKNTEQGAATSVWCAVSEQLAGLGGVYCEDADIAEAVP 303 + DE +P + K TEQGAATSVW AV +L G+GG+Y ED + +A+P Sbjct: 237 -QRALGWLDENDQP-----REGFKTTEQGAATSVWAAVGSELEGVGGLYLEDCN--QALP 288 Query: 304 -ADSVQGSGVWPWAIDREAAERLWVLSEQLTG 334 + +GV P A+D EAA+RLW LS + G Sbjct: 289 WSKENPWTGVMPHALDPEAADRLWDLSVDIVG 320 >VNG0479G [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 316 Score = 142 bits (358), Expect = 7e-34, Method: Compositional matrix adjust. Identities = 108/321 (33%), Positives = 160/321 (49%), Gaps = 36/321 (11%) Query: 33 DLTGKVAIVTGGYSGIGLETSRVLAEAGATVIVPARTPAKAKAA----VSGIP--RLELE 86 D +G+ +VTG SG+G E +R A AGA V++ R+ + + A V+ +P L + Sbjct: 14 DQSGRRVVVTGANSGLGFEATRAFARAGAHVVMACRSTERGEDARDDIVAELPGASLTVH 73 Query: 87 TLDLMDPASIDSFARRFIASGRPLHFLINSAGIMAAPLARDARGYESQFATNHLGHFQLT 146 LDL S+ +FA F A LH L N+AG+MA P + A G+E+QF NHLGH LT Sbjct: 74 ELDLAALDSVAAFADWFTAEFDSLHVLANNAGVMAIPRSETADGFETQFGVNHLGHVALT 133 Query: 147 ARLWPALRQ-ADQARVISVSSRAHRLGGVDLSDPNFERREYDKWKSYAQSKSANVLFAVA 205 A L LR+ + + RV++ SS AHR G +D D E EY KW++Y+QSK AN+LFA Sbjct: 134 AGLLGVLRRTSGETRVVTQSSGAHRRGRIDFEDLQHE-AEYGKWEAYSQSKLANLLFAYE 192 Query: 206 LDE--KAKVYGVRAFAVHPGLIPSSGIGRFLKTEEVGVKPVKVQPAQEDEKGKPASNTNS 263 LD +A V + A HPG ++ R + ++ + ++ A N+ Sbjct: 193 LDRRLRAASASVTSVACHPGYAATNLQLRGPQAAGSRLRLLAMRAA------------NA 240 Query: 264 DFMKNTEQGAATSVWCAVSEQL-----AGLGGVYCEDADIAEAVPADSVQGSGVWPWAID 318 ++ EQGA ++ A + + G GGV P+ + D Sbjct: 241 LVGQSAEQGAWPLLYAATNPSIDGGEYIGPGGVLNMRGHPERQQPSARSR---------D 291 Query: 319 REAAERLWVLSEQLTGVKFTI 339 + A RLW +S TGV F + Sbjct: 292 EDTARRLWTVSADRTGVDFDL 312 >ML0315 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 127 Score = 122 bits (307), Expect = 7e-28, Method: Compositional matrix adjust. Identities = 0/315 (0%), Positives = 0/315 (0%), Gaps = 30/315 (9%) Query: 33 DLTGKVAIVTGGYSGIGLETSRVLAEAGATVIVPARTPAKAKAAVSGIP------RLELE 86 + V + + +++ + + I + Sbjct: 11 KIQKIFLFVKICEKKCRQKELKAFTLIEMLIVLAIISILILLFVPNLIKEKSQVQKTGEA 70 Query: 87 TLDLMDPASIDSFARRFIASGRPLHFLINSAGIMAAPLARDARGYESQFATNHLGHFQLT 146 Sbjct: 71 130 Query: 147 ARLWPALRQADQARVISVSSRAHRL-GGVDLSDPNFERREYDKWKSYAQSKSANVLFAVA 205 Sbjct: 131 - 189 Query: 206 LDEK-AKVYGVRAFAVHPGLIPSSGIGRFLKTEEVGVKPVKVQPAQEDEKGKPASNTNSD 264 Sbjct: 190 ----------------- 232 Query: 265 FMKNTEQGAATSVWCAVSEQLAGLGGVYCEDADIAEAVPADSVQGSGVWPWAIDREAAER 324 Sbjct: 233 ---- 288 Query: 325 LWVLSEQLTGVKFTI 339 Sbjct: 289 303 >Rv0068 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 303 Score = 122 bits (305), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 103/320 (32%), Positives = 151/320 (47%), Gaps = 41/320 (12%) Query: 33 DLTGKVAIVTGGYSGIGLETSRVLAEAGATVIVPARTPAKAKAAVSGIPR------LELE 86 D TG+ A++TG +G+G ET+ LA GA V++ R K K A + I +EL+ Sbjct: 11 DQTGRTAVITGANTGLGFETAAALAAHGAHVVLAVRNLDKGKQAAARITEATPGAEVELQ 70 Query: 87 TLDLMDPASIDSFARRFIASGRPLHFLINSAGIMAAPLARDARGYESQFATNHLGHFQLT 146 LDL AS+ + A + + + + LIN+AG+M P A G+E QF TNHLGHF LT Sbjct: 71 ELDLTSLASVRAAAAQLKSDHQRIDLLINNAGVMYTPRQTTADGFEMQFGTNHLGHFALT 130 Query: 147 ARLWPALRQADQARVISVSSRAHRL-GGVDLSDPNFERREYDKWKSYAQSKSANVLFAVA 205 L L +RV+++SS HR+ + D +ERR Y + +Y Q+K AN+LF Sbjct: 131 GLLIDRLLPVAGSRVVTISSVGHRIRAAIHFDDLQWERR-YRRVAAYGQAKLANLLFTYE 189 Query: 206 LDEKAKVYGVR-AFAVHPGLIPSSGIGRFLKTEEVGVKPVKVQPAQEDEKGKPASNTNSD 264 L + G A A HPG + ++ + R + V V + + Sbjct: 190 LQRRLAPGGTTIAVASHPG-VSNTEVVRNMPRPLVAVAAIL-----------------AP 231 Query: 265 FMKNTEQGAATSVWCAVSEQLAGLGGVYCEDADIAE-----AVPADSVQGSGVWPWAIDR 319 M++ E GA ++ A A GG Y E V A S Q D Sbjct: 232 LMQDAELGALPTLRAATDP--AVRGGQYFGPDGFGEIRGYPKVVASSAQSH-------DE 282 Query: 320 EAAERLWVLSEQLTGVKFTI 339 + RLW +SE+LTGV + + Sbjct: 283 QLQRRLWAVSEELTGVVYPV 302 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.131 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,057,928 Number of extensions: 957650 Number of successful extensions: 2795 Number of sequences better than 1.0e-05: 84 Number of HSP's gapped: 2788 Number of HSP's successfully gapped: 84 Length of query: 339 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 232 Effective length of database: 26,037,693 Effective search space: 6040744776 Effective search space used: 6040744776 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 116 (49.3 bits)