BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0566.0023.p (228 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM1655 [LKJ] COG0513 Superfamily II DNA and RNA helicases 187 9e-48 TM1655 [TK] COG0745 Response regulators consisting of a CheY-lik... 187 9e-48 lin0315 [TK] COG0745 Response regulators consisting of a CheY-li... 183 1e-46 SA0017 [TK] COG0745 Response regulators consisting of a CheY-lik... 183 2e-46 SPy0528 [TK] COG0745 Response regulators consisting of a CheY-li... 177 1e-44 >STM1655 [LKJ] COG0513 Superfamily II DNA and RNA helicases Length = 457 Score = 187 bits (476), Expect = 9e-48, Method: Compositional matrix adjust. Identities = 17/232 (7%), Positives = 39/232 (16%), Gaps = 5/232 (2%) Query: 2 SRILIIDDEVALVLLLTDELQAKGHEVLAAYDGNEGVRLADSEP-DLVILDIMMPGMNGF 60 + L E+ + + + A + + + + L + Sbjct: 12 AAQLNNLTELGYLEMTPVQAAALPVILAGNDVRVQARTGSGKTAAFGLGLLHRIDVTLFQ 71 Query: 61 EVCRSIRDR---VSCPIIFLSARQSEADRVRGLTLGGDDYVIKPFGLRELLARIEANLRR 117 + L + LTL G + I Sbjct: 72 TQALVLCPTRELADQVAGELRRLARFLPNTKILTLCGGQPFGAQRDSLQHAPHIIVATPG 131 Query: 118 EERSRQKPQQQSRLRFGK-LELSLKERLVSVAGEPVALTRREYEITELLALHGGQVFSRE 176 + + S + L + + R T L Sbjct: 132 RLLDHLQKETVSLDALHILVMDEADRMLDMGFSDAIDEVIRFAPATRQTLLFSATWPEAI 191 Query: 177 HIYEKVWGYDAEGDASTVVEHVKKIRAKLAAADPSGDYISTVWGIGYKWTNP 228 V+ + I + + Sbjct: 192 AAISGRVQQQPIRIEIDTVDALPAIEQQFFETSAHEKISLLQTLLSQHQPAS 243 >TM1655 [TK] COG0745 Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain Length = 247 Score = 187 bits (476), Expect = 9e-48, Method: Compositional matrix adjust. Identities = 99/232 (42%), Positives = 152/232 (65%), Gaps = 5/232 (2%) Query: 2 SRILIIDDEVALVLLLTDELQAKGHEVLAAYDGNEGVRLADSEP-DLVILDIMMPGMNGF 60 +IL++DD+ A++ L+ L +G+EVL AYDG E +++A+ E D+ I+DIM+PG++GF Sbjct: 12 KKILVVDDDPAILELVGYNLSKEGYEVLKAYDGEEALKIANDEDVDMFIVDIMLPGIDGF 71 Query: 61 EVCRSIRDR---VSCPIIFLSARQSEADRVRGLTLGGDDYVIKPFGLRELLARIEANLRR 117 E+ R IR + P+IFLSA+ E D+V GL LG DDY+ KPF +RELLAR++A RR Sbjct: 72 ELVRKIRSMEKYKNTPVIFLSAKGEEFDKVLGLELGADDYITKPFSVRELLARVKAIFRR 131 Query: 118 EERSRQKPQQQSRLRFGK-LELSLKERLVSVAGEPVALTRREYEITELLALHGGQVFSRE 176 + Q +++ + K LE+ +++ V V G+ V LT E+E+ LA + G+VFSR+ Sbjct: 132 LSTATQSKEERPKKIIAKDLEIDVEKYEVKVRGKKVNLTPLEFELLRFLAENEGKVFSRD 191 Query: 177 HIYEKVWGYDAEGDASTVVEHVKKIRAKLAAADPSGDYISTVWGIGYKWTNP 228 + +K+WGYD GD TV H++++R K+ + YI TV G GYK+ +P Sbjct: 192 VLLDKLWGYDYYGDTRTVDVHIRRLRTKIEEDPSNPKYIITVRGKGYKFRDP 243 >lin0315 [TK] COG0745 Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain Length = 237 Score = 183 bits (465), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 97/231 (41%), Positives = 142/231 (61%), Gaps = 4/231 (1%) Query: 2 SRILIIDDEVALVLLLTDELQAKGHEVLAAYDGNEGVRLADS-EPDLVILDIMMPGMNGF 60 +IL++DDE + ++ L +G +V AYDG+E + L + +PDL++LDIM+PG +G Sbjct: 4 KKILVVDDEKPIADIVKFNLNKEGFDVYCAYDGDEALELVEEVQPDLILLDIMLPGRDGI 63 Query: 61 EVCRSIRDRVSCPIIFLSARQSEADRVRGLTLGGDDYVIKPFGLRELLARIEANLRREER 120 EVCR +R + PII ++A+ SE D+V GL LG DDYV KPF REL+AR++ANLRR + Sbjct: 64 EVCREVRKKYDMPIIMVTAKDSEIDKVIGLELGADDYVTKPFSNRELIARVKANLRRHSQ 123 Query: 121 ---SRQKPQQQSRLRFGKLELSLKERLVSVAGEPVALTRREYEITELLALHGGQVFSREH 177 + + ++ S L G L + + S GE + LT RE+E+ LA H GQV +REH Sbjct: 124 VSSNAAEEEENSELEIGSLIIHPDAYVASKRGETIELTHREFELLHYLAKHMGQVMTREH 183 Query: 178 IYEKVWGYDAEGDASTVVEHVKKIRAKLAAADPSGDYISTVWGIGYKWTNP 228 + + VWGYD GD TV V+++R K+ ++ T G+GY NP Sbjct: 184 LLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPAWLVTRRGVGYYLRNP 234 >SA0017 [TK] COG0745 Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain Length = 235 Score = 183 bits (465), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 97/231 (41%), Positives = 145/231 (62%), Gaps = 17/231 (7%) Query: 3 RILIIDDEVALVLLLTDELQAKGHEVLAAYDGNEGVRLA-DSEPDLVILDIMMPGMNGFE 61 +++++DDE + +L L+ +G++V AYDGN+ V L + EPD+V+LDIM+PG +G E Sbjct: 6 KVVVVDDEKPIADILEFNLKKEGYDVYCAYDGNDAVDLIYEEEPDIVLLDIMLPGRDGME 65 Query: 62 VCRSIRDRVSCPIIFLSARQSEADRVRGLTLGGDDYVIKPFGLRELLARIEANLRREERS 121 VCR +R + PII L+A+ SE D+V GL LG DDYV KPF REL+AR++ANLR R Sbjct: 66 VCREVRKKYEMPIIMLTAKDSEIDKVLGLELGADDYVTKPFSTRELIARVKANLR---RH 122 Query: 122 RQKPQQQSRLRFGKL--ELSLKERL-------VSVAGEPVALTRREYEITELLALHGGQV 172 +P Q + G + E+++K+ + + GE + LT RE+E+ L+ H GQV Sbjct: 123 YSQPAQDT----GNVTNEITIKDIVIYPDAYSIKKRGEDIELTHREFELFHYLSKHMGQV 178 Query: 173 FSREHIYEKVWGYDAEGDASTVVEHVKKIRAKLAAADPSGDYISTVWGIGY 223 +REH+ + VWGYD GD TV ++++R K+ +YI T G+GY Sbjct: 179 MTREHLLQTVWGYDYFGDVRTVDVTIRRLREKIEDDPSHPEYIVTRRGVGY 229 >SPy0528 [TK] COG0745 Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain Length = 236 Score = 177 bits (449), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 96/230 (41%), Positives = 139/230 (60%), Gaps = 7/230 (3%) Query: 1 VSRILIIDDEVALVLLLTDELQAKGHEVLAAYDGNEGVRLADSE-PDLVILDIMMPGMNG 59 + +ILI+DDE + ++ L +G++++ A+DG E V + + E PDL+ILD+M+P ++G Sbjct: 1 MKKILIVDDEKPISDIIKFNLTKEGYDIVTAFDGREAVTIFEEEKPDLIILDLMLPELDG 60 Query: 60 FEVCRSIRDRVSCPIIFLSARQSEADRVRGLTLGGDDYVIKPFGLRELLARIEANLRREE 119 EV + IR PII LSA+ SE D+V GL +G DDYV KPF RELLAR++A+LRR E Sbjct: 61 LEVAKEIRKTSHVPIIMLSAKDSEFDKVIGLEIGADDYVTKPFSNRELLARVKAHLRRTE 120 Query: 120 RSRQKPQQQS------RLRFGKLELSLKERLVSVAGEPVALTRREYEITELLALHGGQVF 173 +++ L G L++ + G+ V LT RE+E+ LA H GQV Sbjct: 121 TIETAVAEENASSGTQELTIGNLQILPDAFVAKKHGQEVELTHREFELLHHLANHMGQVM 180 Query: 174 SREHIYEKVWGYDAEGDASTVVEHVKKIRAKLAAADPSGDYISTVWGIGY 223 +REH+ E VWGYD GD TV V+++R K+ +YI T G+GY Sbjct: 181 TREHLLEIVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPEYILTRRGVGY 230 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.138 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 16,887,475 Number of extensions: 684096 Number of successful extensions: 6160 Number of sequences better than 1.0e-05: 454 Number of HSP's gapped: 5619 Number of HSP's successfully gapped: 454 Length of query: 228 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 125 Effective length of database: 26,499,029 Effective search space: 3312378625 Effective search space used: 3312378625 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 114 (48.5 bits)