BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0566.0022.p (485 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2727 [T] COG0642 Signal transduction histidine kinase 134 5e-31 CAC0317 [T] COG0642 Signal transduction histidine kinase 132 2e-30 lin2643 [T] COG0642 Signal transduction histidine kinase 116 8e-26 BS_phoR_3 [T] COG0642 Signal transduction histidine kinase 115 2e-25 baeS [T] COG0642 Signal transduction histidine kinase 111 2e-24 >lin2727 [T] COG0642 Signal transduction histidine kinase Length = 182 Score = 134 bits (336), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 0/309 (0%), Positives = 0/309 (0%), Gaps = 16/309 (5%) Query: 163 LTVIFIGALVSPFLYIILFALLFSRVVSNNINKPLRLLMEAARKIKEKDLDFEIGYHSDN 222 Sbjct: 161 ---- 216 Query: 223 ELGQLSSAFDEMKTELESSLSAQWRMEQERVEMVEALAHDLKAPLSIIRSYTEALMDSGG 282 Sbjct: 217 -------- 268 Query: 283 TDDERLQRYLGVIHENAGKSSELVRQMQYTSDMERTDTVLHAETFELAPFLERKIKAYGL 342 Sbjct: 269 328 Query: 343 EAGQKGIDIILRMEDAAGSCLYTDAELLERILDNVVMNSLEYTPSGGSISVSVTLRGSRV 402 Sbjct: 329 --- 385 Query: 403 LYEICDTGPGFSRRDLEKAVQKFYRGDDARASRDGHSGLGLYIAKQLAAKLGGSIRL-SN 461 Sbjct: 386 445 Query: 462 TPEGGACVV 470 Sbjct: 446 454 >CAC0317 [T] COG0642 Signal transduction histidine kinase Length = 498 Score = 132 bits (331), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 76/271 (28%), Positives = 136/271 (50%), Gaps = 1/271 (0%) Query: 188 VVSNNINKPLRLLMEAARKIKEKDLDFEIGYHSDNELGQLSSAFDEMKTELESSLSAQWR 247 +VS +I PL L KIK +LDF++ S +E+G+L AFD M+ +L+ S+ Q + Sbjct: 205 LVSRSILIPLNQLKIGTEKIKHGNLDFKVRAESSDEIGELCDAFDSMRKKLKESVELQAQ 264 Query: 248 MEQERVEMVEALAHDLKAPLSIIRSYTEALMDSGGTDDERLQRYLGVIHENAGKSSELVR 307 E R E++ ++HDLK P++ I+ Y E + D E++++Y+ I+ + L+ Sbjct: 265 YENNRKELISNISHDLKTPITSIKGYIEGIRDGVADTPEKMEKYVNTIYNKTQSMNYLIE 324 Query: 308 QMQYTSDMERTDTVLHAETFELAPFLERKIKAYGLEAGQKGIDIILRMEDAAGSCLYTDA 367 ++ S ++ + + ++ ++ + + + ++ + + D Sbjct: 325 ELLTYSKLDLKKLPFYFIDMDFVSYMNDLMEEFKFDVEKNNMEFSYDNHIKGKANVRIDV 384 Query: 368 ELLERILDNVVMNSLEYT-PSGGSISVSVTLRGSRVLYEICDTGPGFSRRDLEKAVQKFY 426 + + R++ N++ NS++Y G ISV V VL I D G G S L +FY Sbjct: 385 QNINRVISNIISNSIKYMDKEHGKISVEVDSNNEEVLVGIKDNGMGISEEALPYVFDRFY 444 Query: 427 RGDDARASRDGHSGLGLYIAKQLAAKLGGSI 457 R D +R + G SGLGL I K++ + GGSI Sbjct: 445 REDFSRNTSKGGSGLGLAICKKIIEEHGGSI 475 >lin2643 [T] COG0642 Signal transduction histidine kinase Length = 182 Score = 116 bits (290), Expect = 8e-26, Method: Compositional matrix adjust. Identities = 0/221 (0%), Positives = 0/221 (0%) Query: 248 MEQERVEMVEALAHDLKAPLSIIRSYTEALMDSGGTDDERLQRYLGVIHENAGKSSELVR 307 Sbjct: 362 421 Query: 308 QMQYTSDMERTDTVLHAETFELAPFLERKIKAYGLEAGQKGIDIILRMEDAAGSCLYTDA 367 Sbjct: 422 481 Query: 368 ELLERILDNVVMNSLEYTPSGGSISVSVTLRGSRVLYEICDTGPGFSRRDLEKAVQKFYR 427 Sbjct: 482 541 Query: 428 GDDARASRDGHSGLGLYIAKQLAAKLGGSIRLSNTPEGGAC 468 Sbjct: 542 582 >BS_phoR_3 [T] COG0642 Signal transduction histidine kinase Length = 431 Score = 115 bits (287), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 12/220 (5%), Positives = 32/220 (14%), Gaps = 1/220 (0%) Query: 242 LSAQWRMEQERVEMVEALAHDLKAPLSIIRSYTEALMDSGGTDDERLQRYLGVIHENAGK 301 W P S+ L +R + + Sbjct: 41 GLLIWHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRF 100 Query: 302 SSELVRQMQYTSDMERTDTVLHAETFELAPFLERKIKAYGLEAGQKGIDIILRMEDAAGS 361 S + R + G Sbjct: 101 RSGAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKT-R 159 Query: 362 CLYTDAELLERILDNVVMNSLEYTPSGGSISVSVTLRGSRVLYEICDTGPGFSRRDLEKA 421 L+ ++ + + YT + + ++ + S Sbjct: 160 DFSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTPL 219 Query: 422 VQKFYRGDDARASRDGHSGLGLYIAKQLAAKLGGSIRLSN 461 + + + + Sbjct: 220 TVLQGYLEMMNEQPLEGAVREKALHTMREQTQRMEGLVKQ 259 >baeS [T] COG0642 Signal transduction histidine kinase Length = 467 Score = 111 bits (278), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 71/281 (25%), Positives = 136/281 (48%), Gaps = 20/281 (7%) Query: 196 PLRLLMEAARKIKEKDLDFEIGYHSDNELGQLSSAFDEMKTELESSLSAQWRMEQERVEM 255 P++ L++ K+ D + S++ELG+L+ F+++ + LE + +Q R + Sbjct: 192 PVKRLVDGTHKLAAGDFTTRVTPTSEDELGKLAQDFNQLASTLEKN-------QQMRRDF 244 Query: 256 VEALAHDLKAPLSIIRSYTEALMDSGGTDDERLQRY----LGVIHENAGKSSELVRQMQY 311 + ++H+L+ PL+++R EA+ D ++++ + + G ++LV + Sbjct: 245 MADISHELRTPLAVLRGELEAIQDG-------VRKFTPETVASLQAEVGTLTKLVDDLHQ 297 Query: 312 TSDMERTDTVLHAETFELAPFLERKIKAYGLEAGQKGIDIILRMEDAAGSCLYTDAELLE 371 S + +L P LE A+ +G+ + + D+ ++ D + L Sbjct: 298 LSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRGLKLQFSLPDSI--TVFGDRDRLM 355 Query: 372 RILDNVVMNSLEYTPSGGSISVSVTLRGSRVLYEICDTGPGFSRRDLEKAVQKFYRGDDA 431 ++ +N++ NSL YT SGGS+ +S R V D+ PG S L+K ++FYR + + Sbjct: 356 QLFNNLLENSLRYTDSGGSLQISAGQRDKTVRLTFADSAPGVSDDQLQKLFERFYRTEGS 415 Query: 432 RASRDGHSGLGLYIAKQLAAKLGGSIRLSNTPEGGACVVIE 472 R G SGLGL I + G I +++P GG + +E Sbjct: 416 RNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGGVSITVE 456 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.135 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 32,923,347 Number of extensions: 1441805 Number of successful extensions: 5109 Number of sequences better than 1.0e-05: 226 Number of HSP's gapped: 4909 Number of HSP's successfully gapped: 227 Length of query: 485 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 375 Effective length of database: 25,691,691 Effective search space: 9634384125 Effective search space used: 9634384125 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)