BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0565.0017.p (287 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0316 [IQR] COG1028 Dehydrogenases with different specificities... 65 2e-10 Rv0068 [IQR] COG1028 Dehydrogenases with different specificities... 62 1e-09 MT0074 [IQR] COG1028 Dehydrogenases with different specificities... 62 1e-09 SA2365 [IQR] COG1028 Dehydrogenases with different specificities... 59 8e-09 Rv0439c [IQR] COG1028 Dehydrogenases with different specificitie... 56 6e-08 >BH0316 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 312 Score = 64.7 bits (156), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 63/244 (25%), Positives = 105/244 (43%), Gaps = 22/244 (9%) Query: 6 QHRGTALITGANNGIGLELTRKMLAEGWQIIALIRS-SFPGD-DSLVSGAVKSKQLRIYK 63 ++R +ITGAN+GIG E ++ EG +I R+ +F + A + + + + Sbjct: 2 ENRRIVIITGANSGIGKEAAQRFANEGDHVIMACRNIAFSRKVQHDIIAASGNDHVDLLE 61 Query: 64 ADLSDFTALKRALNEIRLQEQQIDLLFNNAG---GSFPELLYSKQGRELHFELQTVVPYI 120 D+S F +++ Q +++D+L +NA P L S G EL F PY+ Sbjct: 62 LDVSSFASIRTFCTTFNSQYKRLDILIHNAAYFNHGEPYRL-SPDGIELTFATNVFGPYL 120 Query: 121 IIMXXXXXXXXXXXXTVVNTSSNAAMTVRH-FDPETL--------EHPTKFKKLLGPYAA 171 + +++ SN ++H FDP+ H TK + Y Sbjct: 121 LTSLLRDRLKQSEDPRILHAGSN---IIKHFFDPKQTFDLDLLQGNHGTKSYSVYKMYCQ 177 Query: 172 SKLALSLWTRELAPQLAAAGIMIRSADPGANNTLRSGKDSGLPFWLKPV---MKLFFPHP 228 SK+AL L T ++A A GI + + + P+W +P+ LFFP P Sbjct: 178 SKMALMLLTFQMAEVFADDGIKVNALQINGAKMSKRTIQKVKPWW-RPIAWGQNLFFPPP 236 Query: 229 SHGA 232 S+ A Sbjct: 237 SYMA 240 >Rv0068 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 303 Score = 62.0 bits (149), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 57/203 (28%), Positives = 96/203 (47%), Gaps = 14/203 (6%) Query: 10 TALITGANNGIGLELTRKMLAEGWQIIALIRSSFPGDDSL--VSGAVKSKQLRIYKADLS 67 TA+ITGAN G+G E + A G ++ +R+ G + ++ A ++ + + DL+ Sbjct: 16 TAVITGANTGLGFETAAALAAHGAHVVLAVRNLDKGKQAAARITEATPGAEVELQELDLT 75 Query: 68 DFTALKRALNEIRLQEQQIDLLFNNAGGSFPELLYSKQGRELHFELQTVVPYIIIMXXXX 127 +++ A +++ Q+IDLL NNAG + + G E+ F + + + Sbjct: 76 SLASVRAAAAQLKSDHQRIDLLINNAGVMYTPRQTTADGFEMQFGTNHLGHFALTGLLID 135 Query: 128 XXXXXXXXTVVNTSS-----NAAMTVRHFDPETLEHPTKFKKLLGPYAASKLALSLWTRE 182 VV SS AA+ HFD L+ ++++ + Y +KLA L+T E Sbjct: 136 RLLPVAGSRVVTISSVGHRIRAAI---HFD--DLQWERRYRR-VAAYGQAKLANLLFTYE 189 Query: 183 LAPQLAAAGIMIRSAD-PGANNT 204 L +LA G I A PG +NT Sbjct: 190 LQRRLAPGGTTIAVASHPGVSNT 212 >MT0074 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 303 Score = 61.6 bits (148), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 57/203 (28%), Positives = 96/203 (47%), Gaps = 14/203 (6%) Query: 10 TALITGANNGIGLELTRKMLAEGWQIIALIRSSFPGDDSL--VSGAVKSKQLRIYKADLS 67 TA+ITGAN G+G E + A G ++ +R+ G + ++ A ++ + + DL+ Sbjct: 16 TAVITGANTGLGFETAAALAAHGAHVVLAVRNLDKGKQAAARITEATPGAEVELQELDLT 75 Query: 68 DFTALKRALNEIRLQEQQIDLLFNNAGGSFPELLYSKQGRELHFELQTVVPYIIIMXXXX 127 +++ A +++ Q+IDLL NNAG + + G E+ F + + + Sbjct: 76 SLASVRAAAAQLKSDHQRIDLLINNAGVMYTPRQTTADGFEMQFGTNHLGHFALTGLLID 135 Query: 128 XXXXXXXXTVVNTSS-----NAAMTVRHFDPETLEHPTKFKKLLGPYAASKLALSLWTRE 182 VV SS AA+ HFD L+ ++++ + Y +KLA L+T E Sbjct: 136 RLLPVAGSRVVTISSVGHRIRAAI---HFD--DLQWERRYRR-VAAYGQAKLANLLFTYE 189 Query: 183 LAPQLAAAGIMIRSAD-PGANNT 204 L +LA G I A PG +NT Sbjct: 190 LQRRLAPGGTTIAVASHPGVSNT 212 >SA2365 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 234 Score = 58.9 bits (141), Expect = 8e-09, Method: Compositional matrix adjust. Identities = 67/224 (29%), Positives = 105/224 (46%), Gaps = 28/224 (12%) Query: 12 LITGANNGIGLELTRKMLAEGWQIIALIRSSFPGDDSLVSGAVKSKQLRIY--KADLSDF 69 LITG N G+G + A G+++ + G + V G S++L ++ + D++ Sbjct: 6 LITGGNKGLGYASAEALKALGYKV-------YIGSRNDVRGQQASQKLGVHYVQLDVTSD 58 Query: 70 TALKRALNEIRLQEQQIDLLFNNAG--GSF--PELLYSKQGRELHFELQTVVPYIIIMXX 125 ++K A N I +E ++D+L NNAG G F P L + E++ QT V I+ M Sbjct: 59 YSVKNAYNMIAEKEGRLDILINNAGISGQFSAPSKLTPRDVEEVY---QTNVFGIVRMMN 115 Query: 126 XXX--XXXXXXXTVVNTSSNAAMTVRHFDPETLEHPTKFKKLLGPYAASKLALSLWTREL 183 VVN SS +PET E +K L Y +SK A+++ T + Sbjct: 116 TFVPLLEKSEQPVVVNVSSGLGSFGMVTNPETAE--SKVNSL--AYCSSKSAVTMLTLQY 171 Query: 184 APQLAAAGIMIRSADPGANNTLRSGKDSG----LPFWLKPVMKL 223 A L + I +ADPGA NT G S + +KP+++L Sbjct: 172 AKGL--PNMQINAADPGATNTDLVGDFSNNSKHVSEGIKPIIQL 213 >Rv0439c [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 311 Score = 56.2 bits (134), Expect = 6e-08, Method: Compositional matrix adjust. Identities = 61/234 (26%), Positives = 100/234 (42%), Gaps = 22/234 (9%) Query: 12 LITGANNGIGLELTRKMLAEGWQIIALIRSSFPGDDSLVSGAVK--SKQLRIYKADLSDF 69 ++TGAN GIG G ++ +R+ G+ + + + + DL Sbjct: 25 VVTGANTGIGYHTAAVFADRGAHVVLAVRNLEKGNAARARIMAARPGAHVTLQQLDLCSL 84 Query: 70 TALKRALNEIRLQEQQIDLLFNNAGGSFPELLYSKQGRELHFELQTVVPYIIIMXXXXXX 129 +++ A + +R +ID+L NNAG + +K G EL F + + + Sbjct: 85 DSVRAAADALRTAYPRIDVLINNAGVMWTPKQVTKDGFELQFGTNHLGHFALTGLVLDHM 144 Query: 130 XXXXXXTVVNTSS-----NAAMTVRHFDPETLEHPTKFKKLLGPYAASKLALSLWTRELA 184 VV SS +AA+ HFD L+ ++ + + Y +KLA L+T EL Sbjct: 145 LPVPGSRVVTVSSQGHRIHAAI---HFD--DLQWERRYNR-VAAYGQAKLANLLFTYELQ 198 Query: 185 PQLAAAG--IMIRSADPGANNTLRSGKDSGLPFWLKPVMK----LFFPHPSHGA 232 +L AG + +A PG +NT + LP ++PV L F P GA Sbjct: 199 RRLGEAGKSTIAVAAHPGGSNTELT---RNLPRLIRPVATVLGPLLFQSPEMGA 249 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.135 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,148,708 Number of extensions: 696904 Number of successful extensions: 1827 Number of sequences better than 1.0e-05: 43 Number of HSP's gapped: 1850 Number of HSP's successfully gapped: 43 Length of query: 287 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 182 Effective length of database: 26,268,361 Effective search space: 4780841702 Effective search space used: 4780841702 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)