BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0565.0015.p (365 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gldA [C] COG0371 Glycerol dehydrogenase and related enzymes 327 2e-89 ZgldA [C] COG0371 Glycerol dehydrogenase and related enzymes 327 2e-89 ECs4874 [C] COG0371 Glycerol dehydrogenase and related enzymes 327 3e-89 STM4108 [C] COG0371 Glycerol dehydrogenase and related enzymes 325 5e-89 >gldA [C] COG0371 Glycerol dehydrogenase and related enzymes Length = 380 Score = 327 bits (839), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 172/363 (47%), Positives = 231/363 (63%), Gaps = 2/363 (0%) Query: 1 MTQIIGAPFKYIQGSGEVSRLGLYCSQLGAKGVYAVVDPFVRSLYGEEITRSFEQHSVAL 60 M +II +P KYIQG+ ++RLG Y L + + V D FV + +SF+ + + Sbjct: 14 MDRIIQSPGKYIQGADVINRLGEYLKPLAERWL-VVGDKFVLGFAQSTVEKSFKDAGLVV 72 Query: 61 VQEEFRGECSIEQVERXXXXXXXXXXXXXXXXXXXXKTLDTAKAVSHFAGLPVVLVPTVA 120 F GECS +++R KTLDTAKA++HF G+PV + PT+A Sbjct: 73 EIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGG-KTLDTAKALAHFMGVPVAIAPTIA 131 Query: 121 STDAPCSALSVLYTEDGQLDCYLPLRRNPDMVIADVDIIAKAPPRLLAAGMGDALSTYYE 180 STDAPCSALSV+YT++G+ D YL L NP+MVI D I+A AP RLLAAG+GDAL+T++E Sbjct: 132 STDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGAPARLLAAGIGDALATWFE 191 Query: 181 ARACRSAGAVTQAGGSSSIAAFALARACHDTLIAYGAEALRDCREGIGSEAVGRIVEANI 240 ARAC +GA T AGG + AA ALA C++TL+ G +A+ + + + A+ R++EAN Sbjct: 192 ARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAMLAAEQHVVTPALERVIEANT 251 Query: 241 YLSGIGFESGGLAAAHAIHNGLTLLEECRHMLHGEKVAFATLAQLMLEQAPAEEIAEATA 300 YLSG+GFESGGLAAAHA+HNGLT + + H HGEKVAF TL QL+LE AP EEI A Sbjct: 252 YLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVAFGTLTQLVLENAPVEEIETVAA 311 Query: 301 FCRMAGLPVTLGELGLSAAKTDRLMIAAEASCAADNPMGNMPFAVSPQQVLDAMLAADRQ 360 GLP+TL +L + ++ I AEA+CA + NMP +P QV A+L AD+ Sbjct: 312 LSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGETIHNMPGGATPDQVYAALLVADQY 371 Query: 361 GQQ 363 GQ+ Sbjct: 372 GQR 374 >ZgldA [C] COG0371 Glycerol dehydrogenase and related enzymes Length = 380 Score = 327 bits (839), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 171/363 (47%), Positives = 231/363 (63%), Gaps = 2/363 (0%) Query: 1 MTQIIGAPFKYIQGSGEVSRLGLYCSQLGAKGVYAVVDPFVRSLYGEEITRSFEQHSVAL 60 M +II +P KYIQG+ ++RLG Y L + + V D FV + +SF+ + + Sbjct: 14 MDRIIQSPGKYIQGADVINRLGEYLKPLAERWL-VVGDKFVLGFAQSTVEKSFKDAGLVV 72 Query: 61 VQEEFRGECSIEQVERXXXXXXXXXXXXXXXXXXXXKTLDTAKAVSHFAGLPVVLVPTVA 120 F GECS +++R KTLDTAKA++HF G+PV + PT+A Sbjct: 73 EIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGG-KTLDTAKALAHFMGVPVAIAPTIA 131 Query: 121 STDAPCSALSVLYTEDGQLDCYLPLRRNPDMVIADVDIIAKAPPRLLAAGMGDALSTYYE 180 STDAPCSALSV+YT++G+ D YL L NP+MVI D I+A AP RLLAAG+GDAL+T++E Sbjct: 132 STDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGAPARLLAAGIGDALATWFE 191 Query: 181 ARACRSAGAVTQAGGSSSIAAFALARACHDTLIAYGAEALRDCREGIGSEAVGRIVEANI 240 ARAC +GA T AGG + AA ALA C++TL+ G +A+ + + + A+ R++EAN Sbjct: 192 ARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAMLAAEQHVVTPALERVIEANT 251 Query: 241 YLSGIGFESGGLAAAHAIHNGLTLLEECRHMLHGEKVAFATLAQLMLEQAPAEEIAEATA 300 YLSG+GFESGGLAAAHA+HNGLT + + H HGEKVAF TL QL+LE +P EEI A Sbjct: 252 YLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVAFGTLTQLVLENSPVEEIETVAA 311 Query: 301 FCRMAGLPVTLGELGLSAAKTDRLMIAAEASCAADNPMGNMPFAVSPQQVLDAMLAADRQ 360 GLP+TL +L + ++ I AEA+CA + NMP +P QV A+L AD+ Sbjct: 312 LSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGETIHNMPGGATPDQVYAALLVADQY 371 Query: 361 GQQ 363 GQ+ Sbjct: 372 GQR 374 Score = 327 bits (837), Expect = 3e-89, Method: Compositional matrix adjust. Identities = 171/363 (47%), Positives = 231/363 (63%), Gaps = 2/363 (0%) Query: 1 MTQIIGAPFKYIQGSGEVSRLGLYCSQLGAKGVYAVVDPFVRSLYGEEITRSFEQHSVAL 60 M +II +P KYIQG+ ++RLG Y L + + V D FV + +SF+ + + Sbjct: 14 MDRIIQSPGKYIQGADVINRLGEYLKPLAERWL-VVGDKFVLGFAQSTVEKSFKDAGLVV 72 Query: 61 VQEEFRGECSIEQVERXXXXXXXXXXXXXXXXXXXXKTLDTAKAVSHFAGLPVVLVPTVA 120 F GECS +++R KTLDTAKA++HF G+PV + PT+A Sbjct: 73 EIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGG-KTLDTAKALAHFMGVPVAIAPTIA 131 Query: 121 STDAPCSALSVLYTEDGQLDCYLPLRRNPDMVIADVDIIAKAPPRLLAAGMGDALSTYYE 180 STDAPCSALSV+YT++G+ D YL L NP+MVI D I+A AP RLLAAG+GDAL+T++E Sbjct: 132 STDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGAPARLLAAGIGDALATWFE 191 Query: 181 ARACRSAGAVTQAGGSSSIAAFALARACHDTLIAYGAEALRDCREGIGSEAVGRIVEANI 240 ARAC +GA T AGG + AA ALA C++TL+ G +A+ + + + A+ R++EAN Sbjct: 192 ARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAMLAAEQHVVTPALERVIEANT 251 Query: 241 YLSGIGFESGGLAAAHAIHNGLTLLEECRHMLHGEKVAFATLAQLMLEQAPAEEIAEATA 300 YLSG+GFESGGLAAAHA+HNGLT + + H HGEKVAF TL QL+LE +P EEI A Sbjct: 252 YLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVAFGTLTQLVLENSPVEEIETVAA 311 Query: 301 FCRMAGLPVTLGELGLSAAKTDRLMIAAEASCAADNPMGNMPFAVSPQQVLDAMLAADRQ 360 GLP+TL +L + ++ I AEA+CA + NMP +P QV A+L AD+ Sbjct: 312 LSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGETIHNMPGGATPDQVYAALLVADQY 371 Query: 361 GQQ 363 GQ+ Sbjct: 372 GQR 374 >ECs4874 [C] COG0371 Glycerol dehydrogenase and related enzymes Length = 380 Score = 327 bits (837), Expect = 3e-89, Method: Compositional matrix adjust. Identities = 171/363 (47%), Positives = 231/363 (63%), Gaps = 2/363 (0%) Query: 1 MTQIIGAPFKYIQGSGEVSRLGLYCSQLGAKGVYAVVDPFVRSLYGEEITRSFEQHSVAL 60 M +II +P KYIQG+ ++RLG Y L + + V D FV + +SF+ + + Sbjct: 14 MDRIIQSPGKYIQGADVINRLGEYLKPLAERWL-VVGDKFVLGFAQSTVEKSFKDAGLVV 72 Query: 61 VQEEFRGECSIEQVERXXXXXXXXXXXXXXXXXXXXKTLDTAKAVSHFAGLPVVLVPTVA 120 F GECS +++R KTLDTAKA++HF G+PV + PT+A Sbjct: 73 EIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGG-KTLDTAKALAHFMGVPVAIAPTIA 131 Query: 121 STDAPCSALSVLYTEDGQLDCYLPLRRNPDMVIADVDIIAKAPPRLLAAGMGDALSTYYE 180 STDAPCSALSV+YT++G+ D YL L NP+MVI D I+A AP RLLAAG+GDAL+T++E Sbjct: 132 STDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGAPARLLAAGIGDALATWFE 191 Query: 181 ARACRSAGAVTQAGGSSSIAAFALARACHDTLIAYGAEALRDCREGIGSEAVGRIVEANI 240 ARAC +GA T AGG + AA ALA C++TL+ G +A+ + + + A+ R++EAN Sbjct: 192 ARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAMLAAEQHVVTPALERVIEANT 251 Query: 241 YLSGIGFESGGLAAAHAIHNGLTLLEECRHMLHGEKVAFATLAQLMLEQAPAEEIAEATA 300 YLSG+GFESGGLAAAHA+HNGLT + + H HGEKVAF TL QL+LE +P EEI A Sbjct: 252 YLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVAFGTLTQLVLENSPVEEIETVAA 311 Query: 301 FCRMAGLPVTLGELGLSAAKTDRLMIAAEASCAADNPMGNMPFAVSPQQVLDAMLAADRQ 360 GLP+TL +L + ++ I AEA+CA + NMP +P QV A+L AD+ Sbjct: 312 LSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGETIHNMPGGATPDQVYAALLVADQY 371 Query: 361 GQQ 363 GQ+ Sbjct: 372 GQR 374 >STM4108 [C] COG0371 Glycerol dehydrogenase and related enzymes Length = 367 Score = 325 bits (834), Expect = 5e-89, Method: Compositional matrix adjust. Identities = 173/363 (47%), Positives = 228/363 (62%), Gaps = 2/363 (0%) Query: 1 MTQIIGAPFKYIQGSGEVSRLGLYCSQLGAKGVYAVVDPFVRSLYGEEITRSFEQHSVAL 60 M +II +P KYIQG+ ++RLG Y + A V D FV E + +S +++ Sbjct: 1 MDRIIQSPGKYIQGANVIARLGDYLKPM-ANNWLVVGDKFVLGFAEETLRKSLTDAGLSV 59 Query: 61 VQEEFRGECSIEQVERXXXXXXXXXXXXXXXXXXXXKTLDTAKAVSHFAGLPVVLVPTVA 120 F GECS +++R KTLDTAKA++HF +PV + PT+A Sbjct: 60 EIAPFGGECSQNEIDRLRAVAEKSQCGAVLGIGGG-KTLDTAKALAHFMNVPVAIAPTIA 118 Query: 121 STDAPCSALSVLYTEDGQLDCYLPLRRNPDMVIADVDIIAKAPPRLLAAGMGDALSTYYE 180 STDAPCSALSV+YT+ G+ D YL L NP+MVI D I+A AP RLLAAG+GDAL+T++E Sbjct: 119 STDAPCSALSVIYTDAGEFDRYLLLPHNPNMVIVDTQIVAGAPARLLAAGIGDALATWFE 178 Query: 181 ARACRSAGAVTQAGGSSSIAAFALARACHDTLIAYGAEALRDCREGIGSEAVGRIVEANI 240 ARAC +GA T AGG + AA ALA C++TLI G +A+ + + + A+ R++EAN Sbjct: 179 ARACSRSGATTMAGGKCTQAALALAELCYNTLIEEGEKAMLAAEQHVVTPALERVIEANT 238 Query: 241 YLSGIGFESGGLAAAHAIHNGLTLLEECRHMLHGEKVAFATLAQLMLEQAPAEEIAEATA 300 YLSG+GFESGGLAAAHAIHNGLT + + H HGEKVAF TL QL+LE AP EEI A Sbjct: 239 YLSGVGFESGGLAAAHAIHNGLTAIPDAHHYYHGEKVAFGTLTQLVLENAPVEEIETVAA 298 Query: 301 FCRMAGLPVTLGELGLSAAKTDRLMIAAEASCAADNPMGNMPFAVSPQQVLDAMLAADRQ 360 C GLP+TL +L + ++ AEASCA + NMP +P +V A+L AD+ Sbjct: 299 LCHSVGLPITLAQLDIKQDIPAKMRTVAEASCAEGETIHNMPGGATPDEVYAALLVADQY 358 Query: 361 GQQ 363 GQ+ Sbjct: 359 GQR 361 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.133 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,498,065 Number of extensions: 880534 Number of successful extensions: 3203 Number of sequences better than 1.0e-05: 17 Number of HSP's gapped: 3181 Number of HSP's successfully gapped: 17 Length of query: 365 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 257 Effective length of database: 25,922,359 Effective search space: 6662046263 Effective search space used: 6662046263 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)