BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0564.0079.p (290 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0846 [R] COG0561 Predicted hydrolases of the HAD superfamily 165 8e-41 BS_ywpJ [R] COG0561 Predicted hydrolases of the HAD superfamily 155 7e-38 BS_ywtE [R] COG0561 Predicted hydrolases of the HAD superfamily 153 3e-37 SA0517 [R] COG0561 Predicted hydrolases of the HAD superfamily 151 1e-36 BH1746 [R] COG0561 Predicted hydrolases of the HAD superfamily 146 4e-35 >BH0846 [R] COG0561 Predicted hydrolases of the HAD superfamily Length = 292 Score = 165 bits (417), Expect = 8e-41, Method: Compositional matrix adjust. Identities = 105/294 (35%), Positives = 154/294 (52%), Gaps = 11/294 (3%) Query: 2 LIALDMDGTLLNEEGKISPENREAIVHAQSLGHIVIIATGRSYMDAERQLRLVDLECPVV 61 LIA+D+DGTLLNE+ IS EN E++ AQ G V+IATGR+Y D L+ + V+ Sbjct: 4 LIAIDLDGTLLNEKSTISEENTESLQRAQEAGMEVVIATGRAYFDVIDLLKPTGMHPWVI 63 Query: 62 SLNGAVVTLPDRTLAASKPLNKEDIIPALRWMSGIPELYYEVYTEDNVYVELDKR----V 117 NGA + P S PL + I L W+ G YYEV++++ ++ + R + Sbjct: 64 GANGATIHDPTGRRLLSIPLEHKQAIEILHWLEG-KHFYYEVFSDEAIFTPQNGRELLAI 122 Query: 118 RLEKLSELNDEAVPGELRWLLKAMIAKQFQQASVTYVESMEEIWSKDEYVIFKTLAFSLN 177 +++L N + G L AKQF Q +YV S ++ E I+ LAFS + Sbjct: 123 EMDRLVSANPQTDLGTL----SQAAAKQFSQTGFSYVSSYRDLLDT-EVNIYNILAFSFD 177 Query: 178 RELLKEASTRFAAIPGLIITASHVNNIEINHKDANKGSGVGMLAAHYGIPAEQVAVMGDS 237 + L + RF + + S +N E+ H A+KG+ + LA H IP E +GDS Sbjct: 178 DQKLAKGWDRFKQTDNVTLVTSAEHNFELEHPLASKGNALTWLAKHLHIPLEHTVAIGDS 237 Query: 238 YNDLPMFEMAGYRIAMENAAPILKETADFVTGNHAENGVAEGLRHLL-EKRKGQ 290 ND M +AGY IAM NA +K+ + VT ++ E+GV+ + LL KR GQ Sbjct: 238 MNDYSMITIAGYSIAMGNARAEIKQISHEVTLSNYEHGVSHSIERLLAAKRLGQ 291 >BS_ywpJ [R] COG0561 Predicted hydrolases of the HAD superfamily Length = 285 Score = 155 bits (392), Expect = 7e-38, Method: Compositional matrix adjust. Identities = 99/288 (34%), Positives = 153/288 (53%), Gaps = 10/288 (3%) Query: 2 LIALDMDGTLLNEEGKISPENREAIVHAQSLGHIVIIATGRSYMDAERQLRLVDLECPVV 61 LIA+D+DGTLLN + ++S EN A+ AQ G V+++TGR++ D + ++ V+ Sbjct: 3 LIAIDLDGTLLNSKHQVSLENENALRQAQRDGIEVVVSTGRAHFDVMSIFEPLGIKTWVI 62 Query: 62 SLNGAVVTLPDRTLAASKPLNKEDIIPALRWMSGIPELYYEVYTEDNVYVELDKR----V 117 S NGAV+ P+ L + ++K+ L W+ YYEV+T +Y + R V Sbjct: 63 SANGAVIHDPEGRLYHHETIDKKRAYDILSWLES-ENYYYEVFTGSAIYTPQNGRELLDV 121 Query: 118 RLEKLSELNDEAVPGELRWLLKAMIAKQFQQASVTYVESMEEIWSKDEYVIF-KTLAFSL 176 L++ N EA +LK Q+ Q+ Y+ S +E++ DE + F L FS Sbjct: 122 ELDRFRSANPEADLS----VLKQAAEVQYSQSGFAYINSFQELFEADEPIDFYNILGFSF 177 Query: 177 NRELLKEASTRFAAIPGLIITASHVNNIEINHKDANKGSGVGMLAAHYGIPAEQVAVMGD 236 +E L+ R+ L + +S +N E++ + A+KG + LA IP E+ A +GD Sbjct: 178 FKEKLEAGWKRYEHAEDLTLVSSAEHNFELSSRKASKGQALKRLAKQLNIPLEETAAVGD 237 Query: 237 SYNDLPMFEMAGYRIAMENAAPILKETADFVTGNHAENGVAEGLRHLL 284 S ND M E AG +AM NA +K AD VT + E+GVA ++HLL Sbjct: 238 SLNDKSMLEAAGKGVAMGNAREDIKSIADAVTLTNDEHGVAHMMKHLL 285 >BS_ywtE [R] COG0561 Predicted hydrolases of the HAD superfamily Length = 286 Score = 153 bits (387), Expect = 3e-37, Method: Compositional matrix adjust. Identities = 103/283 (36%), Positives = 151/283 (53%), Gaps = 4/283 (1%) Query: 2 LIALDMDGTLLNEEGKISPENREAIVHAQSLGHIVIIATGRSYMDAERQLRLVDLECPVV 61 IA+D+DGTLLN+E IS ENREAI A G +V I TGR+ D + L DL+ P++ Sbjct: 3 CIAIDLDGTLLNKESVISAENREAIKRAVDAGILVTICTGRATFDVKALLD--DLDIPII 60 Query: 62 SLNGAVVTLPDRTLAASKPLNKEDIIPALRWMSGIPELYYEVYTEDNVYVELDKRVRLEK 121 + NG + L + +++E ++ +Y+EVYT+D++ D +L Sbjct: 61 AANGGTIHDTGYRLISRTLMDQEAGKAIADYLLS-KNIYFEVYTDDHLLSPFDGEAKLHA 119 Query: 122 LSELNDEAVPGELRWLLKAMIAKQFQQASVTYVESMEEIWSKDEYVIFKTLAFSLNRELL 181 ++ A P E L QF+Q + + +E ++ E I+K L FS + + L Sbjct: 120 ELDILKSANPNEQTDDLWQGAMTQFKQFGIKPIPHIESVFDGGEN-IYKLLCFSFDMDKL 178 Query: 182 KEASTRFAAIPGLIITASHVNNIEINHKDANKGSGVGMLAAHYGIPAEQVAVMGDSYNDL 241 K+A L T+S + IEI + KG + LA YGI + + +GDS NDL Sbjct: 179 KQAKEELKHHKKLAQTSSGKHIIEILPASSGKGRALTKLADIYGIETQDIYAIGDSPNDL 238 Query: 242 PMFEMAGYRIAMENAAPILKETADFVTGNHAENGVAEGLRHLL 284 MFE+AG+RIAMENA LKE + FVT ++ ENGVA + LL Sbjct: 239 SMFEVAGHRIAMENAIDELKEKSTFVTKSNDENGVAYFIDQLL 281 >SA0517 [R] COG0561 Predicted hydrolases of the HAD superfamily Length = 289 Score = 151 bits (381), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 100/287 (34%), Positives = 155/287 (54%), Gaps = 2/287 (0%) Query: 2 LIALDMDGTLLNEEGKISPENREAIVHAQSLGHIVIIATGRSYMDAERQLRLVDLECPVV 61 LIA DMDGTLLN +IS N +AI +AQ G V+IATGR++ +A+ + DL P + Sbjct: 4 LIATDMDGTLLNAAHEISQPNIDAIKYAQEQGITVVIATGRAFYEAQAPVADTDLTVPYI 63 Query: 62 SLNGAVVTLPDRTLAASKPLNKEDIIPALRWMSGIPELYYEVYTEDNVYVELDKRVRLEK 121 LNGA V + ++ LNK ++ + + +YY+VYT +Y E D + L+ Sbjct: 64 CLNGAEVRDETFNVMSTSHLNKS-LVHKITNVLKDAGIYYQVYTSRAIYTE-DPQRDLDI 121 Query: 122 LSELNDEAVPGELRWLLKAMIAKQFQQASVTYVESMEEIWSKDEYVIFKTLAFSLNRELL 181 ++ + A +K I ++ ++ V++ + I + +I K LAF N E + Sbjct: 122 YIDIAERAGQHANVERIKNGIQRRIDNGTLKVVDNYDAIENIPGELIMKILAFDGNLEKI 181 Query: 182 KEASTRFAAIPGLIITASHVNNIEINHKDANKGSGVGMLAAHYGIPAEQVAVMGDSYNDL 241 +AS A P L I++S NIEI H DA KG + +A GI + V +GD+ NDL Sbjct: 182 DKASKILAESPNLAISSSSRGNIEITHSDAQKGIALETIAERLGIEMKDVMAIGDNLNDL 241 Query: 242 PMFEMAGYRIAMENAAPILKETADFVTGNHAENGVAEGLRHLLEKRK 288 M E GY +AMEN A +K+ A +VT + +GV + + LL +++ Sbjct: 242 SMLEKVGYPVAMENGAEEVKKIAKYVTDTNENSGVGKAIMKLLREQQ 288 >BH1746 [R] COG0561 Predicted hydrolases of the HAD superfamily Length = 282 Score = 146 bits (369), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 95/284 (33%), Positives = 151/284 (53%), Gaps = 4/284 (1%) Query: 2 LIALDMDGTLLNEEGKISPENREAIVHAQSLGHIVIIATGRSYMDAERQLRLVDLECPVV 61 LIA DMDGTLLN+E KIS EN EAI AQ G V++ATGR + +A L+ L CP++ Sbjct: 3 LIATDMDGTLLNDERKISKENAEAIKAAQQDGIHVVVATGRDFKEASMPLKEAGLTCPIL 62 Query: 62 SLNGAVVTLPDRTLAASKPLNKEDIIPALRWMSGIPELYYEVYTEDNVYVELDKRVRLEK 121 +NGA + L + L K + + L ++ ++Y+EVYT + + D LE Sbjct: 63 CVNGAEERSANGELIHAVSLAKNEALTILDILTA-ADVYFEVYTNRGAFTD-DYEKGLEI 120 Query: 122 LSELNDEAVPGELRWLLKAMIAKQFQQASVTYVESMEEIWSKDEYVIFKTLAFSLNRELL 181 + ++ A + ++ + +F+ +V E ++ D ++K LAFS + Sbjct: 121 VIDVLQSAGSDDSYEKMREIAKHRFESGAVQLTEDYTKLI--DSTAVYKFLAFSRDETKR 178 Query: 182 KEASTRFAAIPGLIITASHVNNIEINHKDANKGSGVGMLAAHYGIPAEQVAVMGDSYNDL 241 + A ++AS +N+EI H A KG + LA GI ++ V +GD+ ND+ Sbjct: 179 QAAKDDLEKKDVFSVSASASDNLEITHWQAQKGIALRRLAERLGINSDDVMAIGDNLNDV 238 Query: 242 PMFEMAGYRIAMENAAPILKETADFVTGNHAENGVAEGLRHLLE 285 M + AG +AMEN +K+ ADFVT ++ +NGVA+ + L+ Sbjct: 239 SMLKYAGTAVAMENGEAEVKQLADFVTASNEDNGVAKAINKWLK 282 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.134 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,413,664 Number of extensions: 864825 Number of successful extensions: 2486 Number of sequences better than 1.0e-05: 93 Number of HSP's gapped: 2451 Number of HSP's successfully gapped: 110 Length of query: 290 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 185 Effective length of database: 26,268,361 Effective search space: 4859646785 Effective search space used: 4859646785 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 115 (48.9 bits)