BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0564.0074.p (775 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yjcD [L] COG0210 Superfamily I DNA and RNA helicases 365 e-100 BH2834 [L] COG0210 Superfamily I DNA and RNA helicases 360 5e-99 SA1721 [L] COG0210 Superfamily I DNA and RNA helicases 286 7e-77 BH0648 [L] COG0210 Superfamily I DNA and RNA helicases 284 4e-76 CAC2674 [L] COG0210 Superfamily I DNA and RNA helicases 284 5e-76 >BS_yjcD [L] COG0210 Superfamily I DNA and RNA helicases Length = 449 Score = 365 bits (938), Expect = e-100, Method: Compositional matrix adjust. Identities = 0/701 (0%), Positives = 0/701 (0%), Gaps = 70/701 (9%) Query: 17 RIPQAKVASARTSREM--------VKPG--------DSDAAYFRRLEEGGILLNSPQIAA 60 A A + + + + +A Sbjct: 89 ISLTAFTAFSLVLGQHISVPVALGAVFLMGVLFTVISATGIRS-----WILRNLPHGVAH 143 Query: 61 VRHHQGPLLTLAGAGSGKTSVLICRTGYLLSVRGISPSQLLLLTFSNKAAAEMRERIALL 120 L L A +G V+ L G + ++++ A E++ + Sbjct: 144 GTGIGIGLFLLLIAANGVGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVP 203 Query: 121 PGVRESDAARLQARTFHSFFLYFL------RRQGLQQEIFNETRRQHILLKQIMRELGLP 174 G+ + ++F + L Sbjct: 204 GGILLTIIGISIVGLIFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMG--ALNPVVLPSV 261 Query: 175 KDAYPPETLLALLSTCKMNLGEPEKLPETSPAEKEMKAVLLLYEQWKTDNFKIDFDDVLL 234 A + + + + + V + Sbjct: 262 LALVMTAVFDATGTIRAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYI 321 Query: 235 IAYRMLREKPALLLELQMRFLYVMVDEFQDTNALQYELVKMIAHPQHNLMVVGDDDQTIY 294 + L + ++ F + + +++ + I Sbjct: 322 ESAAGTAAGGKTGLTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKID 381 Query: 295 SFNGARSEFILEFEKLYPGARVITLDINYRSGPAIVGLGNGIIRHNMRRRAKTLQAAQGS 354 + + L I I ++G + + A + Sbjct: 382 FADFVDAMAGLVTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVT 441 Query: 355 GRQPRYLRPQTADEEAEQIVEHILSEVASGARAYSDFALLYRASSSNRALLERLLLRDVP 414 Sbjct: 442 - 500 Query: 415 YVDYGEGQLLYEHWLISPVLDYLRLSLNRRNFAAMENILPTLYLNREKGMNHIRRMEAVQ 474 Sbjct: 501 -- 558 Query: 475 PKQGPLIHLLSLPGMEDFKSGKXXXXXXXXXXXXXXTPVQAIR--QIRSGFYDYFIE-AN 531 Sbjct: 559 618 Query: 532 ERHQATLHREMLKEMLDELEASAERFDSLPGFLEFIGNV--TERNELSRQPGLKEQGNRV 589 Sbjct: 619 ------------ 666 Query: 590 ALMTIHKSKGLEFPVVFLIGASEGILPHSSALEGERLKDRKTSPPPGARDSKVIAGNTAG 649 Sbjct: 667 -------------------- 706 Query: 650 LAALEEERRLAYVAVTRAREELFISSPARHRGKKAEVSRFM 690 Sbjct: 707 - 746 >BH2834 [L] COG0210 Superfamily I DNA and RNA helicases Length = 780 Score = 360 bits (924), Expect = 5e-99, Method: Compositional matrix adjust. Identities = 237/668 (35%), Positives = 354/668 (52%), Gaps = 43/668 (6%) Query: 33 VKPGDSDAAYFRRLEEGGILLNSPQIAAVRHHQGPLLTLAGAGSGKTSVLICRTGYLLSV 92 V S + + RL+E GI LN Q A+ +GPLL +AGAGSGKT VL R Y++S Sbjct: 129 VNTNQSRSPFDERLKEQGIFLNDDQWQAITTTEGPLLIIAGAGSGKTRVLTTRAAYMISE 188 Query: 93 RGISPSQLLLLTFSNKAAAEMRERIALLPGVRESDAARLQARTFHSFFLYFLRRQGLQQE 152 + I P +LL+TF+ KAA EM+ER++ + + RL TFHS F ++ Sbjct: 189 QQIDPLSMLLVTFTQKAAREMQERMSTYFTLPTNRLKRLAIGTFHSIFYRIAAHATPEKW 248 Query: 153 IFNETRRQHILLKQIMRE----LGLPKDAYPPETLLALLSTCKMNLGEPEKLPETSPAEK 208 ++ + Q++RE GL + + + L +S K ++ P + S E+ Sbjct: 249 RMDKLLKWDWQRMQLLREHGQKKGLDEKEFAYDQALTQISWWKNHMLHPGDIQPKSTWEE 308 Query: 209 EMKAVLLLYEQWKTDNFKIDFDDVLLIAYRMLREKPALLLELQMRFLYVMVDEFQDTNAL 268 + YE+ + DFDD+L+ AY L + LL Q RF Y+ VDE+QD N + Sbjct: 309 NCLYLYEGYERSRISQQWFDFDDMLIGAYETLTQNHELLARYQERFRYLFVDEYQDINKV 368 Query: 269 QYELVKMIAHPQHNLMVVGDDDQTIYSFNGARSEFILEFEKLYPGARVITLDINYRSGPA 328 QYEL++++ + N+ VVGDDDQ+IYSF G+ +I FE+ Y A+ I L NYRS Sbjct: 369 QYELIRLLTQKEKNICVVGDDDQSIYSFRGSDPSYIQAFERDYEHAKQIVLKDNYRSSHP 428 Query: 329 IVGLGNGIIRHNMRRRAKTLQAAQGSGRQPRYLRPQTADEEAEQIVEHILSEVASGARAY 388 IV +I HN +R AK+++A S + P P +EEA I+ I ++ASG Sbjct: 429 IVMCAQNVIHHNKQRIAKSVRAQYDSEKAPLLFYPYDEEEEATMILSDIQEKIASGTSP- 487 Query: 389 SDFALLYRASSSNRALLERLLLRDVPYVDYGEGQLLYEHWLISPVLDYLRLSLNRRNFAA 448 S FA+LYR + S RA+ ERL+ +VP+ EG+ Y+ + VL YLRLS+N+ + A Sbjct: 488 SAFAILYRTNVSVRAMFERLVASNVPFRMEQEGESFYQRSTVRKVLAYLRLSVNQDDPKA 547 Query: 449 MENILPTLYLNREKGMNHIRRMEAVQPKQGP--LIHLLSLPGMEDFKSGKXXXXXXXXXX 506 ME +LP L+L ++ ++ ++A+ L LL L ++ F+ K Sbjct: 548 MEELLPALFLKKDT----LQELKAITIFHDCSLLDALLHLKNVQPFQQKKLKKLLPYFQK 603 Query: 507 XXXXTPVQAIRQIRS--GFYDYFIE-ANERHQATLHREMLKEMLDELEASAERFDSLPGF 563 +P QAI I G D+ + NE + + LK+++ +A F ++P F Sbjct: 604 LPHLSPTQAISMIEKHMGLTDFIKKRGNEGNVMDRGSDDLKDLI----VAANSFATIPTF 659 Query: 564 LEFIGN-VTERNELSRQPGLKEQGNRVALMTIHKSKGLEFPVVFLIGASEGILPHSSALE 622 L+ + + + + +EL + P +E V LMTIH++KGLEF V+L+ A EG LPH AL+ Sbjct: 660 LDHVSHMIAKHHELKKAPAQEEA---VQLMTIHRAKGLEFQHVYLLSAVEGSLPHDYALD 716 Query: 623 GERLKDRKTSPPPGARDSKVIAGNTAGLAALEEERRLAYVAVTRAREELFISSPARHRGK 682 R D + LEEERRL YVA+TRA+E+LFIS P+ RGK Sbjct: 717 AWREGDDR---------------------PLEEERRLMYVAMTRAQEQLFISVPSMRRGK 755 Query: 683 KAEVSRFM 690 A+ SRF+ Sbjct: 756 NAQRSRFV 763 >SA1721 [L] COG0210 Superfamily I DNA and RNA helicases Length = 730 Score = 286 bits (733), Expect = 7e-77, Method: Compositional matrix adjust. Identities = 200/655 (30%), Positives = 316/655 (48%), Gaps = 84/655 (12%) Query: 53 LNSPQIAAVRHHQGPLLTLAGAGSGKTSVLICRTGYLLSVRGISPSQLLLLTFSNKAAAE 112 +N+ Q AV+ +GPLL +AGAGSGKT VL R YLL + +SP +L +TF+NKAA E Sbjct: 8 MNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYLLDEKDVSPYNVLAITFTNKAARE 67 Query: 113 MRERIALLPGVRESDAARLQARTFHSFFLYFLRRQ----GLQQE--IFNETRRQHILLKQ 166 M+ER+ L G A + TFHS + LRR G+++ I + T ++ ++ K Sbjct: 68 MKERVQKLVG---DQAEVIWMSTFHSMCVRILRRDADRIGIERNFTIIDPTDQKSVI-KD 123 Query: 167 IMRELGLPKDAYPPETLLALLSTCKMNLGEP-EKLPETSPAEKEMKAVLLL-YEQWKTDN 224 +++ + + P + +S K L P + E + +M A + Y++ + N Sbjct: 124 VLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKEATDYHSQMVATVYSGYQRQLSRN 183 Query: 225 FKIDFDDVLLIAYRMLREKPALLLELQMRFLYVMVDEFQDTNALQYELVKMIAHPQHNLM 284 +DFDD+++ + P +L Q +F Y+ VDE+QDTN QY LVK++A NL Sbjct: 184 EALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHVDEYQDTNKAQYTLVKLLASKFKNLC 243 Query: 285 VVGDDDQTIYSFNGARSEFILEFEKLYPGARVITLDINYRSGPAIVGLGNGIIRHNMRRR 344 VVGD DQ+IY + GA + IL FEK YP A I L+ NYRS I+ N +I++N R+ Sbjct: 244 VVGDSDQSIYGWRGADIQNILSFEKDYPEANTIFLEQNYRSTKTILNAANEVIKNNSERK 303 Query: 345 AKTLQAAQGSGRQPRYLRPQTADEEAEQIVEHILSEVASGARAYSDFALLYRASSSNRAL 404 K L A +G + Y T +EAE ++ I+ +G + Y D A+LYR ++ +R L Sbjct: 304 PKGLWTANTNGEKIHYYEAMTERDEAEFVIREIMKHQRNG-KKYQDMAILYRTNAQSRVL 362 Query: 405 LERLLLRDVPYVDYGEGQLLYEHWLISPVLDYLRLSLNRRNFAAMENILPTLYLNREKGM 464 E + ++PY G GQ Y+ I +L YLR+ N + +++ I+ R G Sbjct: 363 EETFMKSNMPYTMVG-GQKFYDRKEIKDLLSYLRIIANSNDDISLQRIINV--PKRGVGP 419 Query: 465 NHIRRMEAVQPKQGPLIHLLSLPGMEDFKSGKXXXXXXXXXXXXXXTPVQAIRQIRSGFY 524 + + +++ + I + G DF + + Q FY Sbjct: 420 SSVEKVQNYALQNN--ISMFDALGEADF-----------------IGLSKKVTQECLNFY 460 Query: 525 DY---------FIEANERHQATLHREMLKEMLDELEA--SAERFDSLPGFLEFIGNVTER 573 + F+E +E L + +EML+ S R +++ F+ + E Sbjct: 461 ELIQSLIKEQEFLEIHEIVDEVLQKSGYREMLERENTLESRSRLENIDEFMSVPKDYEEN 520 Query: 574 NELSRQPGLK--------------EQGNRVALMTIHKSKGLEFPVVFLIGASEGILPHSS 619 L Q + + N V LMT+H +KGLEFP+VF++G E + PH Sbjct: 521 TPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKGLEFPIVFIMGMEESLFPHIR 580 Query: 620 ALEGERLKDRKTSPPPGARDSKVIAGNTAGLAALEEERRLAYVAVTRAREELFIS 674 A++ E + ++EERR+ YVA+TRA E L+I+ Sbjct: 581 AIKSEDDHE------------------------MQEERRICYVAITRAEEVLYIT 611 >BH0648 [L] COG0210 Superfamily I DNA and RNA helicases Length = 747 Score = 284 bits (727), Expect = 4e-76, Method: Compositional matrix adjust. Identities = 208/647 (32%), Positives = 326/647 (50%), Gaps = 63/647 (9%) Query: 53 LNSPQIAAVRHHQGPLLTLAGAGSGKTSVLICRTGYLLSVRGISPSQLLLLTFSNKAAAE 112 LN Q AV+H +GPLL +AGAGSGKT VL R YLL +G++P +L +TF+NKAA E Sbjct: 20 LNDRQREAVKHTEGPLLLMAGAGSGKTRVLTHRIAYLLREKGVAPWNVLAITFTNKAARE 79 Query: 113 MRERIALLPGVRESDAARLQARTFHSFFLYFLRRQ----GLQQEI-FNETRRQHILLKQI 167 M++R+A L G D + TFHS + LRR G + ++ Q ++KQI Sbjct: 80 MKDRVAQLVGPIADD---IWISTFHSMCVRILRRDIDRIGYSRNFTILDSTDQLSVIKQI 136 Query: 168 MRELGLPKDAYPPETLLALLSTCKMNLGEPEKLPETS--PAEKEMKAVLLLYEQWKTDNF 225 ++ + + P ++L +S K L PE+ + P E+ V + Y++ N Sbjct: 137 LKNNNIDSKKFEPRSILGTISGAKNELKTPEQFMGIAKGPYEETAAEVYVEYQKQLKKNQ 196 Query: 226 KIDFDDVLLIAYRMLREKPALLLELQMRFLYVMVDEFQDTNALQYELVKMIAHPQHNLMV 285 +DFDD+++ + ++ P +L Q +F Y++VDE+QDTN QY LVKM+ N+ V Sbjct: 197 ALDFDDLIMKTTSLFKKVPEVLEYYQRKFQYILVDEYQDTNRAQYMLVKMLGDRHKNVCV 256 Query: 286 VGDDDQTIYSFNGARSEFILEFEKLYPGARVITLDINYRSGPAIVGLGNGIIRHNMRRRA 345 VGD DQ+IY + GA IL FE+ Y A VI L+ NYRS I+ N +I +N++R+ Sbjct: 257 VGDSDQSIYKWRGADIANILSFEEDYQDAEVILLEQNYRSTKTILEAANRVIENNVKRKP 316 Query: 346 KTLQAAQGSGRQPRYLRPQTADEEAEQIVEHILSEVASGARAYSDFALLYRASSSNRALL 405 K L G + + + T EA+ +VE I + GA +Y D A+LYR ++ +R + Sbjct: 317 KNLWTENEQGAKIGFYKADTEQGEAQFVVEKIREAIQDGA-SYKDIAILYRTNAQSRVME 375 Query: 406 ERLLLRDVPYVDYGEGQLLYEHWLISPVLDYLRLSLNRRNFAAMENILPTLYLNREKGMN 465 E L+ ++ Y G G Y+ I VL YLRL N + +++ I+ R G Sbjct: 376 EMLIKSNIDYNIVG-GTKFYDRKEIKDVLAYLRLVANPDDDISLQRIINV--PKRGIGAT 432 Query: 466 HIRRMEAVQPKQGPLIHLLSLPGMEDFKSGKXXXXXXXXXXXXXXTPVQ-----AIRQI- 519 + ++ A +QG + +L +E VQ ++ ++ Sbjct: 433 TVDKIAAYAERQG-ISMFAALKEIEQIGVTSRAVSKLVEFTEQLGHWVQMQEYLSVTELV 491 Query: 520 -----RSGFYDYFIEANERHQATLHREMLKEMLDELEASAERFD------SLPGFLEFIG 568 R+G+ D +++ TL + E +DE + + F+ SL FL + Sbjct: 492 EELLERTGYRDML-----QNEKTLEAQSRLENIDEFLSVTQEFEKSSDDKSLIAFLTDLA 546 Query: 569 NVTERNELSRQPGLKEQGNRVALMTIHKSKGLEFPVVFLIGASEGILPHSSALEGERLKD 628 +++ ++L + + + V LMT+H +KGLEFP+VFLIG EGI PHS +L Sbjct: 547 LISDIDKLDDEDDGAKPTDAVVLMTLHSAKGLEFPIVFLIGLEEGIFPHSRSL------- 599 Query: 629 RKTSPPPGARDSKVIAGNTAGLAALEEERRLAYVAVTRAREELFISS 675 +EEERRLAYV +TRA ++L+++S Sbjct: 600 -------------------FEDDEMEEERRLAYVGITRAEKQLYLTS 627 >CAC2674 [L] COG0210 Superfamily I DNA and RNA helicases Length = 756 Score = 284 bits (726), Expect = 5e-76, Method: Compositional matrix adjust. Identities = 201/668 (30%), Positives = 323/668 (48%), Gaps = 95/668 (14%) Query: 52 LLNSPQIAAVRHHQGPLLTLAGAGSGKTSVLICRTGYLLSVRGISPSQLLLLTFSNKAAA 111 LLN Q A GP+L LAGAGSGKT VL R +++ I PSQ+L +TF+NKAA Sbjct: 6 LLNEEQYKAATKIDGPILILAGAGSGKTRVLTYRIAHMVKDLEIYPSQILAITFTNKAAK 65 Query: 112 EMRERIALLPGVRESDAARLQARTFHSFFLYFLRRQ----GLQQEI-FNETRRQHILLKQ 166 EM++R+ L G E D + TFHS + LRR+ G + ++ Q +L+++ Sbjct: 66 EMKDRVKALIGGNEID--NMWISTFHSCCVRILRREIDKIGYNKNFTIYDSDDQKVLMRE 123 Query: 167 IMRELGLPKDAYPPETLLALLSTCKMNLGEPEKLPETSPAE---KEMKAVLLLYEQWKTD 223 ++++G+ + + +++ + + K L + + ++ V LLY++ + Sbjct: 124 CIKQVGINEKDITEKEIISKIGSAKDGLVSANQYKNQNSGNFKLNKIADVYLLYQKKLKE 183 Query: 224 NFKIDFDDVLLIAYRMLREKPALLLELQMRFLYVMVDEFQDTNALQYELVKMIAHPQHNL 283 N +DFDD++ + ++ P +L Q +F Y+M+DE+QDTN QYE +++A NL Sbjct: 184 NNALDFDDLIFKTVELFKKAPDVLAFYQRKFRYIMIDEYQDTNKSQYEFARLLASANKNL 243 Query: 284 MVVGDDDQTIYSFNGARSEFILEFEKLYPGARVITLDINYRSGPAIVGLGNGIIRHNMRR 343 VVGDDDQ IY + GA IL FEK Y A +I L+ NYRS I+ NG+I++N Sbjct: 244 CVVGDDDQCIYGWRGADIRNILNFEKDYRNAVIIKLEENYRSKGNILKAANGVIKNNPHE 303 Query: 344 RAKTLQAAQGSGRQPRYLRPQTADEEAEQIVEHILSEVASGARAYSDFALLYRASSSNRA 403 K+L+ G + + R +T +EA I EV R++ DFA+LYR ++ +R Sbjct: 304 HLKSLRTRSEDGAKIKVYRGETDLDEASFAAFKINKEVKEEKRSFKDFAILYRTNAQSRV 363 Query: 404 LLERLLLRDVPYVDYGEGQLLYEHWLISPVLDYLRLSLNRRNFAAMENILPTLYLN---R 460 + + R++PY G G Y+ I ++ YL+L N ++NI +N R Sbjct: 364 FEDIFIKRNIPYRIIG-GLKFYDRKEIKDIMSYLKLIGN-----PLDNISLKRIINVPKR 417 Query: 461 EKGMNHIRRM-----EAVQPKQGPLIHLLSLPGMEDFKSGKXXXXXXXXXXXXXXTPVQA 515 G + R+ E + L+ + ++PG+ P +A Sbjct: 418 SIGQTTLGRLQQFADEVEECLYDALLDIENIPGI----------------------PKRA 455 Query: 516 IRQIR--SGFYDYFI-EANERHQATLHREMLKEM--LDELEASAERFDS--LPGFLEFIG 568 I I+ +G + FI +E + + L +E+L++ L+EL+AS + D + E + Sbjct: 456 ISSIKEFTGMINSFIRRKDEINVSVLIKEILEQTGYLNELKASDDIQDKARIENIEELVN 515 Query: 569 NVTERNELSRQPGL----------------KEQGNRVALMTIHKSKGLEFPVVFLIGASE 612 + E S L E + V +MT+H +KGLEFPVVF+ G Sbjct: 516 AAVDFEENSDDKTLAAFLEKTALVADVDNYNEDADTVVMMTVHSAKGLEFPVVFMAGMEN 575 Query: 613 GILPHSSALEGERLKDRKTSPPPGARDSKVIAGNTAGLAALEEERRLAYVAVTRAREELF 672 GI P ++ E +EE RRLAYV +TRA+E+L+ Sbjct: 576 GIFPGMASFNSE--------------------------YEMEESRRLAYVGITRAKEKLY 609 Query: 673 ISSPARHR 680 ++S R Sbjct: 610 MTSAKLRR 617 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.136 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 53,370,531 Number of extensions: 2098676 Number of successful extensions: 11001 Number of sequences better than 1.0e-05: 1772 Number of HSP's gapped: 7421 Number of HSP's successfully gapped: 3520 Length of query: 775 Length of database: 38,378,431 Length adjustment: 114 Effective length of query: 661 Effective length of database: 25,230,355 Effective search space: 16677264655 Effective search space used: 16677264655 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 120 (50.8 bits)