BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0564.0067.p (380 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MA2002 [R] COG0596 Predicted hydrolases or acyltransferases (alp... 97 4e-20 MA0362 [R] COG0596 Predicted hydrolases or acyltransferases (alp... 84 5e-16 CAC0202 [R] COG0596 Predicted hydrolases or acyltransferases (al... 62 1e-09 BH1839 [R] COG0596 Predicted hydrolases or acyltransferases (alp... 54 4e-07 PA3829 [R] COG1073 Hydrolases of the alpha/beta superfamily 53 1e-06 >MA2002 [R] COG0596 Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) Length = 417 Score = 97.1 bits (240), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 74/253 (29%), Positives = 111/253 (43%), Gaps = 15/253 (5%) Query: 22 YQRWYGRFLLFGTDYGRLRRVVARVS--DWHKWCEVWTAEGDEVYRKAEKALAEGSEGKA 79 + R G + G D RV D W W + ++ LA + A Sbjct: 31 FLRTIGYAVSGGADINECLDTAYRVKNGDIESWYLEWNRTAGRLETTGDEFLARSNNVSA 90 Query: 80 RALFHEAVACYHAGQHIFFI----DSRQKEAAQEKARNSYVQAISLYNDTEKPVRVDIPY 135 R + A Y F++ D + E++R+ +++A L N+ +PVR IP+ Sbjct: 91 RETYLRASNYYRTAG--FYLTKNPDDPRIVPTWERSRDCFLKAADLSNNLIRPVR--IPF 146 Query: 136 RGVSIPGYLRKADTKG--RPLIIFVNGMDNIKEAEGHHFGSVFNRQGFNFFTFDGPGQGE 193 ++PGYL DT G RPL+I G D E +G+N F+GPGQGE Sbjct: 147 ENTTLPGYLCLVDTDGEKRPLLIVQTGYDGTGEELYFQVAKFAVDRGYNVLIFEGPGQGE 206 Query: 194 MWSSLKFDAR-DYHKAVSAVIDWFEQQPPCGIDLGRIGLVGFSLGGYLAPEAAARDPRVK 252 + K R ++ V+ V ++ Q +D RI L+G S GGYLAP A + R+K Sbjct: 207 VIRVQKIPFRYNWESVVTPVTNYALSQ--SIVDPERIVLMGISFGGYLAPRAVVFEHRIK 264 Query: 253 CTVGNSGLVYIGG 265 + N G+ GG Sbjct: 265 ACIANGGVYDFGG 277 >MA0362 [R] COG0596 Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) Length = 422 Score = 83.6 bits (205), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 74/249 (29%), Positives = 111/249 (44%), Gaps = 14/249 (5%) Query: 34 TDYGRLRRVVARVS--DWHKWCEVWTAEGDEVYRKAEKALAEGSEGKARALFHEAVACYH 91 +D G + VV + D +W W A + +AE+A + A F A + Sbjct: 71 SDLGEVLEVVGHLKPGDEGRWINAWGAMAQRLQYRAEEAERADKQITASTSFLRASTYWR 130 Query: 92 AGQHIFFI--DSRQKEAAQEKARNSYVQAISLYNDTEKPVRVDIPYRGVSIPGYLRKADT 149 A F DSR + A+ ++ Y + + L + V+IPY +PG+ ++ Sbjct: 131 ASLMNFSQPNDSRIGDYARASSK-CYERYLELSGYPGQ--YVEIPYENSFLPGHFYRSPV 187 Query: 150 --KGRPLIIFVNGMDNIKEAEGHHFGSVFNRQGFNFFTFDGPGQGEMWSSLKFDAR-DYH 206 PL+I G D E + R+G + +DGPGQG R D+ Sbjct: 188 AVDNAPLLIITPGRDTWAEDTRWVYDGAI-RRGIHCLVYDGPGQGYALRLNNMTFRPDWE 246 Query: 207 KAVSAVIDWFEQQPPCGIDLGRIGLVGFSLGGYLAPEAAARDPRVKCTVGNSGLVYIGGI 266 VS VID+ P GID RIGL+G S GG+LAP AAA + R+K + + G + GG Sbjct: 247 NVVSPVIDFALTIP--GIDPSRIGLMGLSFGGFLAPRAAAFEKRIKLLIADPGNISWGG- 303 Query: 267 RGLKELNPI 275 + L P+ Sbjct: 304 SIISRLKPV 312 >CAC0202 [R] COG0596 Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) Length = 388 Score = 62.4 bits (150), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 54/223 (24%), Positives = 93/223 (41%), Gaps = 29/223 (13%) Query: 39 LRRVVARVSDWHKWCEVWTAEGDEVYRKAEKALAEGSEGKARALFHEAVACYHAGQHIFF 98 +R + + ++ ++ E + E+Y KA L EG H+ A Y+ F+ Sbjct: 45 MREISPSIHNYEEFIEKFL----ELYNKA---LNEG---------HKLRAAYYLRTAEFY 88 Query: 99 IDSRQKEAAQEKARNSYVQAISLYNDTEKPVRVDIPYRGVSIPGYLRKADTKGRPLIIFV 158 + + + ++ R ++ + Y + +PY +P Y R + I+ Sbjct: 89 MTADHPQ--KQNYRKQFISLMQEYFNISDEQPYKVPYENGFLPAY-RFTSEAPKGTIVMF 145 Query: 159 NGMDNIKEAEGHHFGSVFNRQGFNFFTFDGPGQGEMWSSLKFD-ARDYHKAVSAVIDWFE 217 G D+ E E G++ FDGPGQG K ++ K V +++D+FE Sbjct: 146 GGFDSYVE-EMFKMALFLKEAGYDVVFFDGPGQGSALEDYKIPMTHEWEKPVKSILDYFE 204 Query: 218 QQPPCGIDLGRIGLVGFSLGGYLAPEAAARDPRVKCTVGNSGL 260 L + L+G SLGGYL AAA + RV + + L Sbjct: 205 --------LDDVTLLGISLGGYLTVRAAAFEKRVHRVIADDIL 239 >BH1839 [R] COG0596 Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) Length = 361 Score = 54.3 bits (129), Expect = 4e-07, Method: Compositional matrix adjust. Identities = 66/244 (27%), Positives = 117/244 (47%), Gaps = 23/244 (9%) Query: 85 EAVACYHAGQHIFFIDSRQKEAAQEKARNSYVQAISLYNDTEKPVRVDIPYRGVSIPGYL 144 +A+ CY+ + F S Q+EAA ++ + QA D +K RV + V++ Sbjct: 64 DAIQCYNFARFPFPKTSLQQEAA-DRLTKVFEQAYC-REDVQKH-RVYYDNQKVTVYAAN 120 Query: 145 RKADTKGRPLIIFVNGMDNIKEAEGHHFGSVFNRQGFNFFTFDGPGQGEMWSSLKFDARD 204 D P ++ + G+ +IKE + F V R GF+ + PG GE + +++ A D Sbjct: 121 LHPDL---PCLLVLGGIVSIKE-QWSVFLQVGKRLGFSVLITECPGVGE--NQVRYHA-D 173 Query: 205 YHKAVSAVIDWFEQQPPCGIDLGRIGLVGFSLGGYLAPEAAARDPRVK--CTVGNSGLVY 262 ++ + ++D F Q +G +VG S GG+LA + A D R+K TVG + L Y Sbjct: 174 SYRMIGQILDTFAGQA----KVGDTYIVGMSFGGHLAMKQALHDERIKGITTVG-APLHY 228 Query: 263 IGGIRGLKELNP--IWQRGVTYITGCET--LEAALPQFNYDIGDAPALRSPLLFYHAGRD 318 + +LN I Q + ++ E + AL +F + + L+ P+ + + +D Sbjct: 229 F--FQDYSQLNVPFITQATLAHVMEEELDHVGRALSRFAFMPKELNKLKIPITYIFSSKD 286 Query: 319 EVMP 322 E++P Sbjct: 287 EIIP 290 >PA3829 [R] COG1073 Hydrolases of the alpha/beta superfamily Length = 307 Score = 52.8 bits (125), Expect = 1e-06, Method: Compositional matrix adjust. Identities = 40/137 (29%), Positives = 59/137 (43%), Gaps = 7/137 (5%) Query: 130 RVDIPYR--GVSIPGYLRKADTKG-RPLIIFVNGMDNIKEAEGHHFGSVFNRQGFNFFTF 186 R+++ +R GV++ G+L + + RP I+ +G +KE + VF GF + Sbjct: 3 RLNVEFRSEGVALRGWLYLPEARAERPAIVMTHGFSGVKEQYLDRYAEVFAAAGFVVLLY 62 Query: 187 DGP--GQGEMWSSLKFDARDYHKAVSAVIDWFEQQPPCGIDLGRIGLVGFSLGGYLAPEA 244 D P G + + D + I W Q +D RIG+ G S G E Sbjct: 63 DHPNFGDSDGEPRQEIDPVMQRRGYRDAITWLGAQ--ARVDASRIGIWGTSYSGGHVLEV 120 Query: 245 AARDPRVKCTVGNSGLV 261 AA D RVKC V V Sbjct: 121 AALDRRVKCVVAQVPTV 137 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.139 0.441 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 30,897,585 Number of extensions: 1391233 Number of successful extensions: 11665 Number of sequences better than 1.0e-05: 6 Number of HSP's gapped: 11671 Number of HSP's successfully gapped: 6 Length of query: 380 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 272 Effective length of database: 25,922,359 Effective search space: 7050881648 Effective search space used: 7050881648 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 117 (49.7 bits)