BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0564.0054.p (342 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ydbI [R] COG0628 Predicted permease 258 9e-69 lin0948 [R] COG0628 Predicted permease 257 2e-68 lin1458 [R] COG0628 Predicted permease 257 2e-68 CAC2432 [R] COG0628 Predicted permease 221 1e-57 L197809 [R] COG0628 Predicted permease 67 4e-11 >BS_ydbI [R] COG0628 Predicted permease Length = 350 Score = 258 bits (659), Expect = 9e-69, Method: Compositional matrix adjust. Identities = 143/346 (41%), Positives = 210/346 (60%), Gaps = 16/346 (4%) Query: 1 MDVFKRYYANLTIRRFXXXXXXXXXXYSIRDMLNLVLLTFLIAYVMNSFQVLLSKRIGKY 60 M +++ + +RRF Y + M+NL+LLTF+ ++M+ ++++ + + ++ Sbjct: 1 MAALVKFFQHPGVRRFSIFVVLTGVLYLFKSMINLILLTFIFTFLMDRLELVVRQFVSQF 60 Query: 61 VKVNSKVIIVVLYLALITMIVLALVKYLPKVFTQIKQXXXXXXXXXXXXXXQ---NEVTQ 117 +V+ +V+I LY+ L ++ + + P V QI+Q +E+T Sbjct: 61 FRVSQRVVITFLYMLLAVLLTVGGFVFYPVVAAQIQQLVKQIKHIAYHPDSIPFFDEIT- 119 Query: 118 YIFNQLKDLNYESYVTHGIG----YVLRISNWGTTFVLATILSFVFILEKNRIVSFTSRL 173 + D+N SYV G Y+ IS +G V+A ILS F+ EK R+ F ++ Sbjct: 120 ---SVFGDINISSYVKEGFNVVYTYLADISTFGLQVVMALILSMFFLFEKKRLSEFMAKF 176 Query: 174 RDSKISWFYVELEYFGKKFISSFGKVIEAQILIALFNTMFTVIGLWILGFFFEPFPYLFA 233 + SK+ FY E+ +FG KF +FGKV+EAQ +IAL N + T I LWI+ F P LF Sbjct: 177 KTSKLRVFYEEIAFFGSKFARTFGKVLEAQFIIALVNCILTFIALWIMHF-----PQLFG 231 Query: 234 LSIMIFLLSLIPVVGFVISLIPLCIIAYNIGGLAMTLYVLGLIAVLHFMEGYFLNPKLMS 293 LSIM+F L LIPV G VISLIPL IIAY+ GG LY++ +I +H +E YFLNPKLMS Sbjct: 232 LSIMVFFLGLIPVAGVVISLIPLSIIAYSTGGGMYVLYIVLVIFAIHAIETYFLNPKLMS 291 Query: 294 SKMNLPMFYTFIVLLFSEHYIGVWGLILGIPIFVFFLDILDITREK 339 +K LP+F+TF VL+FSEH+ G+WGLI+GIPIFVF LDILD+T ++ Sbjct: 292 AKTELPIFFTFTVLIFSEHFFGIWGLIIGIPIFVFLLDILDVTNKE 337 >lin0948 [R] COG0628 Predicted permease Length = 348 Score = 257 bits (657), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 141/343 (41%), Positives = 216/343 (62%), Gaps = 14/343 (4%) Query: 1 MDVFKRYYANLTIRRFXXXXXXXXXXYSIRDMLNLVLLTFLIAYVMNSFQVLLSKRIGKY 60 M+ ++ N T RR Y +R M++L+LLTF+ A+++ + ++ KRI Sbjct: 1 MNWLEKLKENDTARRVLVFVLLGIVLYLLRSMIDLILLTFIFAFLVTRLENVILKRI--- 57 Query: 61 VKVNSKVIIVVLYLALITMIVLALVKYLPKVFTQIKQXXXXXXXXXXXXXXQNEVTQYIF 120 ++ K+I++VLY + + +A+V +LP + QI Q N++ Q+I Sbjct: 58 -RIPRKLIVIVLYCLVAMFLYVAIVHFLPILIDQISQLVDSLVKIYNNPS-DNKIVQWIV 115 Query: 121 NQLKDLNYESYVTHGIGYVLR----ISNWGTTFVLATILSFVFILEKNRIVSFTSRLRDS 176 LK+ N + Y+ G+ +++ I + G +F LA ILS F LEK R+ SFT + S Sbjct: 116 GFLKESNIQKYLQTGVDFIIASLSGIGSVGLSFFLALILSLFFSLEKERVTSFTGQFLTS 175 Query: 177 KISWFYVELEYFGKKFISSFGKVIEAQILIALFNTMFTVIGLWILGFFFEPFPYLFALSI 236 K+ + + E +FGKKF+++FG V+EAQ++IAL NT+ T I L+++ F P L +LSI Sbjct: 176 KVGFIFKEAAFFGKKFVATFGVVLEAQLMIALVNTIITTIALYLMNF-----PQLLSLSI 230 Query: 237 MIFLLSLIPVVGFVISLIPLCIIAYNIGGLAMTLYVLGLIAVLHFMEGYFLNPKLMSSKM 296 M+F+L LIPV G +IS +PL +IAY++GG +Y+L + ++H +E Y LNPKLMSSK Sbjct: 231 MVFVLGLIPVAGVIISCVPLVLIAYSVGGFQDVVYILITVVIVHAIETYILNPKLMSSKT 290 Query: 297 NLPMFYTFIVLLFSEHYIGVWGLILGIPIFVFFLDILDITREK 339 NLP+FYTFI+L+FSE + GVWGLI+GIP+FVF LDILD+ + Sbjct: 291 NLPVFYTFIILIFSETFFGVWGLIVGIPVFVFLLDILDVRNSE 333 >lin1458 [R] COG0628 Predicted permease Length = 345 Score = 257 bits (656), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 141/343 (41%), Positives = 211/343 (61%), Gaps = 14/343 (4%) Query: 1 MDVFKRYYANLTIRRFXXXXXXXXXXYSIRDMLNLVLLTFLIAYVMNSFQVLLSKRIGKY 60 M F + N ++RR Y +R +N++LLTF+ +Y++ + + +RI Y Sbjct: 1 MKEFNLFLQNRSVRRVGVFLLIAFVLYLLRSQMNIILLTFIFSYLITRLENFILRRISIY 60 Query: 61 VKVNSKVIIVVLYLALITMIVLALVKYLPKVFTQIKQXXXXXXXXXXXXXXQNEVTQYIF 120 ++I+++LY+ + +IV VKY+P + QI Q NE YI Sbjct: 61 ----RQIIVLLLYVLIAGVIVFVFVKYIPVLADQINQLVKFGNSFLKTDS-NNEFINYIV 115 Query: 121 NQLKDLNYESYVTHGIGYVLR-ISNWGTTFV---LATILSFVFILEKNRIVSFTSRLRDS 176 + + Y G+ +L ++N GT + +A +LS F L K+ ++SFT++ S Sbjct: 116 SLANQFDIMKYTEQGVSMILTYLTNVGTVLMNVFIALMLSLFFSLGKDHLISFTNQFSTS 175 Query: 177 KISWFYVELEYFGKKFISSFGKVIEAQILIALFNTMFTVIGLWILGFFFEPFPYLFALSI 236 KI + Y E+++FG KF+++FGKVIEAQ +IAL N + T I LWILGF P L LSI Sbjct: 176 KIGFIYEEVKFFGSKFVATFGKVIEAQFIIALVNAVLTTIALWILGF-----PQLMTLSI 230 Query: 237 MIFLLSLIPVVGFVISLIPLCIIAYNIGGLAMTLYVLGLIAVLHFMEGYFLNPKLMSSKM 296 M+FLL LIPV G +ISLIPL II Y+IGG+ Y+L ++ ++H +E Y LNPKLMS+K Sbjct: 231 MVFLLGLIPVAGVIISLIPLTIIGYSIGGVEYIFYILVVVIIIHALESYVLNPKLMSAKT 290 Query: 297 NLPMFYTFIVLLFSEHYIGVWGLILGIPIFVFFLDILDITREK 339 NLP+FYTFI+L+F EH+ G+WGLI+GIP+ +FFLD+L +T ++ Sbjct: 291 NLPVFYTFIILIFGEHFFGIWGLIVGIPVVMFFLDVLGVTNQE 333 >CAC2432 [R] COG0628 Predicted permease Length = 339 Score = 221 bits (564), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 126/315 (40%), Positives = 189/315 (60%), Gaps = 11/315 (3%) Query: 27 YSIRDMLNLVLLTFLIAYVMNSFQVLLSKRIGKYVKVNSKVIIVVLYLALITMIVLALVK 86 Y R ++N +LLTF++ Y+++S + + KY+KV +I ++LY+ +I +IVL Sbjct: 27 YFCRSIMNFILLTFIVTYLISSLHGFIVSNLRKYIKVREGLIALLLYVLIIAVIVLVFYN 86 Query: 87 YLPKVFTQIKQXXXXXXXXXXXXXXQNEVT--QYIFNQLKDLNYESYVTHGIGYVLRIS- 143 Y+P + + + T +YI + L+ SY G+ Y++ + Sbjct: 87 YVPVIVRETTNIVNQATNFYNTIDTSDLGTLGKYIQPMITKLDIGSYTKQGLSYIIEFAK 146 Query: 144 NWGT---TFVLATILSFVFILEKNRIVSFTSRLRDSKISWFYVELEYFGKKFISSFGKVI 200 N+G LA +LS F+LEK I F + SK+S Y Y+G F++SFGKVI Sbjct: 147 NFGKVSLNLFLAFVLSLFFVLEKGTIKKFGKKFEKSKLSTMYEYFVYYGNNFLNSFGKVI 206 Query: 201 EAQILIALFNTMFTVIGLWILGFFFEPFPYLFALSIMIFLLSLIPVVGFVISLIPLCIIA 260 +AQILIA+ N + + I L I+GF P L L MIF+LSLIPV G +ISL+PL IA Sbjct: 207 KAQILIAIVNAVLSSIALAIMGF-----PNLMTLGFMIFVLSLIPVAGVIISLVPLSFIA 261 Query: 261 YNIGGLAMTLYVLGLIAVLHFMEGYFLNPKLMSSKMNLPMFYTFIVLLFSEHYIGVWGLI 320 +NI G +YV+ ++ V+H +E YFLNPKLMSSK LP+F+TFI+L+ S ++G WGL+ Sbjct: 262 FNINGFTEVIYVILMVVVIHCIESYFLNPKLMSSKTKLPIFFTFIILIVSGDFMGTWGLL 321 Query: 321 LGIPIFVFFLDILDI 335 LGIP+F+F LD+ ++ Sbjct: 322 LGIPLFMFLLDVFEV 336 >L197809 [R] COG0628 Predicted permease Length = 313 Score = 67.0 bits (162), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 58/225 (25%), Positives = 109/225 (48%), Gaps = 27/225 (12%) Query: 113 NEVTQYIFNQLKD------LNYESYVTHGIGYVLRISNWGTTFVLATILSFVFIL----- 161 ++ + I NQL D +N + ++H I TT +L +++ F F+L Sbjct: 73 DQFAKSIGNQLIDWSKAFSVNAVTTLSHLISK--------TTSILISLIVFPFVLFYLLR 124 Query: 162 EKNRIVSFTSRLRDSKISWFYVELEYFGKKFISSFGKVIEAQILIALFNTMFTVIGLWIL 221 + R+ F + L + + E + S + Q+L+A+ + +IGL I+ Sbjct: 125 DGQRLNGFVTHLLPND---WRKETSKVLHEINSQLSNYVRGQVLVAIAVAIMFMIGLPII 181 Query: 222 GFFFEPFPYLFALSIMIFLLSLIPVVGFVISLIPLCIIAYNIGGLAMTLYVLGLIAVLHF 281 G Y AL+I L+L+P +G ++ IP+ I+ IGG M + V+ ++ + Sbjct: 182 GLR-----YAVALAITAGFLNLVPFLGSFLAAIPMVIVGLVIGGPLMLVKVIIVLIIEQT 236 Query: 282 MEGYFLNPKLMSSKMNLPMFYTFIVLLFSEHYIGVWGLILGIPIF 326 +EG F++P ++ +M++ VLL + +GVWG++L IP + Sbjct: 237 LEGRFISPLVLGKQMSIHPITILFVLLTAGQILGVWGVLLAIPFY 281 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.334 0.149 0.443 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,218,244 Number of extensions: 874801 Number of successful extensions: 4013 Number of sequences better than 1.0e-05: 57 Number of HSP's gapped: 3979 Number of HSP's successfully gapped: 57 Length of query: 342 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 235 Effective length of database: 26,037,693 Effective search space: 6118857855 Effective search space used: 6118857855 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.6 bits) S2: 116 (49.3 bits)