BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0564.0053.p (287 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value VNG0364G [IQR] COG1028 Dehydrogenases with different specificiti... 109 4e-24 CAC3335 [IQR] COG1028 Dehydrogenases with different specificitie... 104 2e-22 L142816 [IQR] COG1028 Dehydrogenases with different specificitie... 98 2e-20 PA1379 [IQR] COG1028 Dehydrogenases with different specificities... 97 2e-20 MA2687 [IQR] COG1028 Dehydrogenases with different specificities... 96 6e-20 >VNG0364G [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 273 Score = 109 bits (273), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 79/245 (32%), Positives = 110/245 (44%), Gaps = 11/245 (4%) Query: 9 MGKIICITXXXXXXXXXXXXKFAKEGWTVYAGTRNLERDQERYREVEHLTFVELEVTKPE 68 M K+ +T E W V A R+ D E EL+VTKP Sbjct: 1 MEKVALVTGCSSGVGAATVRSLTDEEWRVVATARDTA-DLVALAEETGCETAELDVTKPA 59 Query: 69 SIQRVIGQIEQEQDKLDVLFCNAGFGYLRALGQAPVEDIHRVFDTNVYGVMHTIRAALPL 128 + V+ + +LD L NAGF L L P +HR FD NVYG IRAALP Sbjct: 60 QCRAVVDDAVDDHGRLDCLVNNAGFAQLGPLEDVPPRKLHRQFDVNVYGPHRLIRAALPH 119 Query: 129 LRKTGYGHIVATSSVGGLVGQPMNEIYCASKFALEGLLESMATYYKPQFNIDISLLEPGA 188 +R+ G G IV SSV G V P Y SKFALEGL +++ + ID++++EPG Sbjct: 120 MRERGEGTIVNVSSVSGRVATPGMGAYNGSKFALEGLSDALRGEVA-EHGIDVAVVEPGP 178 Query: 189 IATEFNNTV---LGHVSETGGILDDEYKPVIEAYTQAFLQRNVEPQTADSVAEVMWNLVH 245 + T F + L + +GG Y+ + + A V + + VA+V+ Sbjct: 179 VDTGFQDRADAELDALDRSGG-----YERIYSWFEDASAVNGVGAVSPERVADVIATAA- 232 Query: 246 MEPKP 250 + P P Sbjct: 233 VSPNP 237 >CAC3335 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 266 Score = 104 bits (259), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 55/165 (33%), Positives = 95/165 (57%), Gaps = 5/165 (3%) Query: 33 EGWTVYAGTRNLERDQERYREVEHLTFVELEVTKPESIQRVIGQIEQEQDKLDVLFCNAG 92 +G+TV A R +E +++ + L+VTK ES+ + I ++ K+D+L NAG Sbjct: 27 KGYTVVATARKVED----LKDLPAALKLPLDVTKEESVHKAIKEVMLNFHKIDILVNNAG 82 Query: 93 FGYLRALGQAPVEDIHRVFDTNVYGVMHTIRAALPLLRKTGYGHIVATSSVGGLVGQPMN 152 + AL + V D+ +F+ NV+G+++ ++A +P +RK G I+ S+ G QP+N Sbjct: 83 YSVRGALEEVNVSDVKSMFNVNVFGIINMLQAVIPEMRKRKTGKIINIGSISGKFVQPIN 142 Query: 153 EIYCASKFALEGLLESMATYYKPQFNIDISLLEPGAIATEFNNTV 197 YCA+KFA+E L +++ +NI +++EPG I T F T+ Sbjct: 143 GAYCAAKFAVEALSDALRLELS-SYNIQTTVIEPGPIKTNFFKTL 186 >L142816 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 270 Score = 97.8 bits (242), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 58/193 (30%), Positives = 99/193 (51%), Gaps = 3/193 (1%) Query: 12 IICITXXXXXXXXXXXXKFAKEGWTVYAGTRNLERDQERYREVEHLTFVELEVTKPESIQ 71 II IT A+ G VY R +++ E+ + V + + L++T ES + Sbjct: 3 IILITGASSGIGYQTAELLAQNGHKVYGAARRMDK-LEKLKSV-GVEPIYLDITNEESCR 60 Query: 72 RVIGQIEQEQDKLDVLFCNAGFGYLRALGQAPVEDIHRVFDTNVYGVMHTIRAALPLLRK 131 +++ I + ++D+L NAG+G A+ P+E+ R F+ N++G+ + LP +R Sbjct: 61 KLVDTIIDNEGQIDILINNAGYGSYGAIEDVPLEEAKRQFEVNLFGLAALTKLVLPHMRH 120 Query: 132 TGYGHIVATSSVGGLVGQPMNEIYCASKFALEGLLESMATYYKPQFNIDISLLEPGAIAT 191 G I+ SS+GG Y A+K+ALE +++ KP F ID+SL+EPG I T Sbjct: 121 QKAGRIINISSMGGRFTTYFGAWYHATKYALEAFSDALRMEVKP-FGIDVSLIEPGGIKT 179 Query: 192 EFNNTVLGHVSET 204 + H++E+ Sbjct: 180 NWGLIAADHLAES 192 >PA1379 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 277 Score = 97.4 bits (241), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 59/206 (28%), Positives = 105/206 (50%), Gaps = 4/206 (1%) Query: 62 LEVTKPESIQRVIGQIEQEQDKLDVLFCNAGFGYLRALGQAPVEDIHRVFDTNVYGVMHT 121 L+VT +I+ + IE++ LDVL +AG+G+ L ++P+ ++ R F+ N++G + Sbjct: 57 LDVTDLAAIEPTVAAIERDIGPLDVLVNSAGYGHEGILEESPLAEMRRQFEVNLFGAVAM 116 Query: 122 IRAALPLLRKTGYGHIVATSSVGGLVGQPMNEIYCASKFALEGLLESMATYYKPQFNIDI 181 I+A LP +R+ GHI+ +S+GG + P YC SKFALEG+ E++ I + Sbjct: 117 IQAVLPYMRRRRRGHILNITSMGGYITMPGIAYYCGSKFALEGVSEALGKEVA-GLGIAV 175 Query: 182 SLLEPGAIATEFNNTVLGHVSETGGILDDEYKPVIEAYTQAFLQRNVEPQTADSVAEVMW 241 + + PG+ T++ + T D + PV +A + ++ +P+ A A M Sbjct: 176 TAVAPGSFRTDWAGRSMVRSPRTIADYDALFDPVRQARQEKSGKQPGDPRKA---ARAML 232 Query: 242 NLVHMEPKPLRIRTSEQAENFVSRKV 267 + E P + A V +K+ Sbjct: 233 QAIDAENPPAHLLLGSDALGLVRQKL 258 >MA2687 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 242 Score = 95.9 bits (237), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 54/172 (31%), Positives = 95/172 (55%), Gaps = 6/172 (3%) Query: 30 FAKEGWTVYAGTRN---LERDQERYREVEHLTF-VELEVTKPESIQRVIGQIEQEQDKLD 85 AKEG + RN + Q++ +++ F + ++V E ++R+I + + +LD Sbjct: 31 LAKEGVNIIIAARNESESKETQDQLKDMGSKAFEIPVDVRNEEDVRRLISRTIDKCGRLD 90 Query: 86 VLFCNAGFGYLRALGQAPVEDIHRVFDTNVYGVMHTIRAALPLLRKTGYGHIVATSSVGG 145 +L NAG + L + VE+ ++ DTN+ GV + A+P LR++ G I+ SS+GG Sbjct: 91 ILINNAGVALKKRLEETTVEEYDKIIDTNLKGVFLCTKYAIPYLRRSKNGKIINISSIGG 150 Query: 146 LVGQPMNEIYCASKFALEGLLESMATYYKPQFNIDISLLEPGAIATEFNNTV 197 L G P +YCASKF + G+ E++A + Q I + + PGA+ T+ ++ Sbjct: 151 LHGIPEFSVYCASKFGVNGVTEAIAAELEGQ--IKVYSICPGAVDTDMYRSI 200 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.134 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,874,190 Number of extensions: 832229 Number of successful extensions: 3109 Number of sequences better than 1.0e-05: 187 Number of HSP's gapped: 3105 Number of HSP's successfully gapped: 187 Length of query: 287 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 182 Effective length of database: 26,268,361 Effective search space: 4780841702 Effective search space used: 4780841702 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)