BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0564.0031.p (404 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2165 [R] COG0673 Predicted dehydrogenases and related proteins 72 2e-12 BH0710 [R] COG0673 Predicted dehydrogenases and related proteins 71 3e-12 lin2266 [R] COG0673 Predicted dehydrogenases and related proteins 70 6e-12 BH1248 [R] COG0673 Predicted dehydrogenases and related proteins 69 2e-11 lin2262 [R] COG0673 Predicted dehydrogenases and related proteins 68 3e-11 >BH2165 [R] COG0673 Predicted dehydrogenases and related proteins Length = 348 Score = 72.0 bits (175), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 43/153 (28%), Positives = 80/153 (52%), Gaps = 2/153 (1%) Query: 1 MTQQPIRLAIVGGNRGSRFYDLLAWIA--DRIRLAAICDVDDRILSNWKEQYPSIQTYQN 58 M Q+ +R+ I+G +R + ++++ D++ L AI DV + EQ+ Y + Sbjct: 1 MEQKKLRVGIIGAGGIARTVHIPSYLSLKDQVELVAITDVQPKHAQAAAEQFSIPYVYND 60 Query: 59 YEQLLQNPDIDAVFLASPMLLHARQSIDALKAGKHVLSEVIAAHTVEEAWELVETVEQTG 118 +++Q ++DAV + +P H +I+AL AG HVL E A TVE+A ++ ++TG Sbjct: 61 ASRMMQEVELDAVSICTPNAFHRELTIEALHAGCHVLCEKPPAITVEDAKQMEAAAKETG 120 Query: 119 LVYMMAENYCFSRTSLMIRHMAEQGLFGEITYM 151 + +Y S ++ ++G GEI ++ Sbjct: 121 NLLTYGFHYRHSPEVETLKRFVDEGELGEIYHV 153 >BH0710 [R] COG0673 Predicted dehydrogenases and related proteins Length = 388 Score = 71.2 bits (173), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 47/179 (26%), Positives = 90/179 (50%), Gaps = 10/179 (5%) Query: 4 QPIRLAIVG----GNRGSRFY-DLLAWIADRIR--LAAICDVDDRILSNWKEQYPSIQTY 56 + I++ ++G G S Y DL + D +R + IC + ++ QY + Sbjct: 2 ESIKVGMIGYKFMGKAHSHAYRDLPMFFPDHLRPEMKVICGRNPEGVAQAASQYGWEEYT 61 Query: 57 QNYEQLLQNPDIDAVFLASPMLLHARQSIDALKAGKHVLSEVIAAHTVEEAWELVETVEQ 116 N++ L++ DID + + +P +H ++ A +AGKH+ E A T++++ ++E VE Sbjct: 62 TNWKDLIRRDDIDLIDINAPSDVHKEITLAAAQAGKHIFCEKPLALTLQDSRHMLEAVEA 121 Query: 117 TGLVYMMAENYCFSRTSLMIRHMAEQGLFGEITYMEGGYIHDLRALVHQPDGSLNWRGQ 175 G+ +M+ NY F+ L+ + + +G G+I + ++ D + PD L WR Q Sbjct: 122 AGVKHMVGFNYRFTPAVLLAKKLIAEGRLGDIYHFRAWFLQD---WIVAPDFPLVWRLQ 177 >lin2266 [R] COG0673 Predicted dehydrogenases and related proteins Length = 358 Score = 70.1 bits (170), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 45/156 (28%), Positives = 74/156 (47%), Gaps = 7/156 (4%) Query: 6 IRLAIVGGN---RGSRFYDLLAWIADRIRLAAICDVDDRILSNWKEQYPS--IQTYQNYE 60 +++ I+G G LL A++ + A CD+ +++ S Y NY Sbjct: 3 LKVGIIGCGGIANGKHMPSLLK--AEKAEMVAFCDIVLEKAEAAAKEFGSENASVYTNYV 60 Query: 61 QLLQNPDIDAVFLASPMLLHARQSIDALKAGKHVLSEVIAAHTVEEAWELVETVEQTGLV 120 +LLQ+ ID + + +P + HA SI A++AGKHV+ E A T EA ++E +TG Sbjct: 61 ELLQDKSIDVIHVCTPNISHAEISIAAMEAGKHVMCEKPMAKTTAEAKSMIEAANRTGKK 120 Query: 121 YMMAENYCFSRTSLMIRHMAEQGLFGEITYMEGGYI 156 + F + S + + E G G+I Y + I Sbjct: 121 LTIGYQNRFRKDSDYLHQVCENGELGDIYYAKAKAI 156 >BH1248 [R] COG0673 Predicted dehydrogenases and related proteins Length = 340 Score = 68.6 bits (166), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 46/157 (29%), Positives = 77/157 (49%), Gaps = 7/157 (4%) Query: 4 QPIRLAIVGGNRGSRFYDLLAWIAD-RIRLAAICDVDDRILSNWKEQYPSIQTYQNYEQL 62 + +R+A++G ++ L + + + A+CD + + Y +I Y ++ QL Sbjct: 2 KTLRVAVIGCGSIAQHRHLPEYEQNPHTTIVAVCDPVEERAKKVAKLYGAI-AYTDHMQL 60 Query: 63 LQNPDIDAVFLASPMLLHARQSIDALKAGKHVLSEVIAAHTVEEAWELVETVEQTGLVYM 122 L+ +ID V + +P HA SIDALKAG HVL E A + EE +++ E+ G+ M Sbjct: 61 LEKEEIDLVSVCTPNAYHAPISIDALKAGTHVLCEKPMATSAEECEAMIKAAEEAGMCLM 120 Query: 123 MAENYCFSRTSLMIRHMAEQGLFGEI-----TYMEGG 154 + N F + R + G G+I T+ GG Sbjct: 121 IGHNQRFVSSHEKARQLIATGDIGKIYSFRTTFGHGG 157 >lin2262 [R] COG0673 Predicted dehydrogenases and related proteins Length = 349 Score = 67.8 bits (164), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 46/149 (30%), Positives = 81/149 (54%), Gaps = 8/149 (5%) Query: 7 RLAIVG-GNRGSRFYDLLAWIADRIRLAAICDVDDRILSNWKEQ--YPSIQTYQNYEQLL 63 +L IVG G GS ++ LA AD + + + D IL+ +E ++ Y++YE +L Sbjct: 5 QLVIVGYGGMGS-YHVTLASAADNLEVHGVFD----ILAEKREAAAQKGLKIYESYEAVL 59 Query: 64 QNPDIDAVFLASPMLLHARQSIDALKAGKHVLSEVIAAHTVEEAWELVETVEQTGLVYMM 123 + +DAV +A+P H +I AL+AGKHV+ E T E+ +++ ++ +M+ Sbjct: 60 ADEKVDAVLIATPNDSHKELAISALEAGKHVVCEKPVTMTSEDLLAIMDVAKRVNKHFMV 119 Query: 124 AENYCFSRTSLMIRHMAEQGLFGEITYME 152 +N + L+I+ M EQ GE+ ++E Sbjct: 120 HQNRRWDEDFLIIKEMFEQKTIGEMFHLE 148 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.135 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,584,503 Number of extensions: 1323221 Number of successful extensions: 3774 Number of sequences better than 1.0e-05: 59 Number of HSP's gapped: 3773 Number of HSP's successfully gapped: 59 Length of query: 404 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 295 Effective length of database: 25,807,025 Effective search space: 7613072375 Effective search space used: 7613072375 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)