BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0564.0021.p (271 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value FN1891 [C] COG0584 Glycerophosphoryl diester phosphodiesterase 54 3e-07 RSc1268 [C] COG0584 Glycerophosphoryl diester phosphodiesterase 51 2e-06 STM3553 [C] COG0584 Glycerophosphoryl diester phosphodiesterase 49 7e-06 lin0625_2 [C] COG0584 Glycerophosphoryl diester phosphodiesterase 49 9e-06 >FN1891 [C] COG0584 Glycerophosphoryl diester phosphodiesterase Length = 261 Score = 53.5 bits (127), Expect = 3e-07, Method: Compositional matrix adjust. Identities = 58/249 (23%), Positives = 105/249 (42%), Gaps = 24/249 (9%) Query: 26 ITAHTGCM-ETLDNTLDAVRMGIELGADIVEDDVRVTKDGIAVMAHDDIWT---TAAGRS 81 I AH G +NTL A++ IE+ AD +E D+++TKDG V+ HD W T GR Sbjct: 24 IFAHRGASGYAPENTLTAIKKAIEMKADGIEIDIQLTKDGKIVVIHD--WKVDRTTTGRG 81 Query: 82 ISISETNFTELKNVEVAVDYAGKQGSMTFSTLEEMLRLIKPTGTIANXXXXXXXXXXXXX 141 + E +F +++++ Y TLEE+L ++ P + N Sbjct: 82 F-VYELDFDYIRSLDAGQWYTKDFVGEVVPTLEEVLDVL-PNDMMLNIEIKDTARKHSNI 139 Query: 142 XXXHSLDML-------EQVFLSGCEKERALLAQRRQPQLRKLLNADIRLFRTLPYEFAVE 194 L++L E + +S ++ Q +P+L+ L D Y Sbjct: 140 EEK-MLEVLKKYPEKFEDIIVSSFHHDKIKRLQELEPKLKLALLTDSEFIEIEKY----- 193 Query: 195 QTCREALEASCFGINIYHEVVTPWFMERARASGLPVYVWTVNETQLMEHYADLGVASITA 254 L++ + I +++ +E + + V+VWTVN+ + +++ LGV + Sbjct: 194 -LSTNGLKSYSYHPEI--NLISKKDVEILHKNDVKVFVWTVNKEEDLDYLVTLGVDGVIT 250 Query: 255 RNVQALADL 263 + +L Sbjct: 251 NYPDIMKEL 259 >RSc1268 [C] COG0584 Glycerophosphoryl diester phosphodiesterase Length = 252 Score = 51.2 bits (121), Expect = 2e-06, Method: Compositional matrix adjust. Identities = 54/225 (24%), Positives = 92/225 (40%), Gaps = 16/225 (7%) Query: 22 PFPFITAHTGCME-TLDNTLDAVRMGIELGADIVEDDVRVTKDGIAVMAHDDIW---TTA 77 P+P AH G + +NTL A R G G + E DV+++ DG+AV+ HD T+ Sbjct: 11 PYPRAIAHRGAGKLAPENTLAAFRHGAGFGYRMFEFDVKLSGDGVAVLLHDATLERTTSG 70 Query: 78 AGRSISISETNFTELKNVEVAVDYAGKQGSMTFSTLEEMLRLIKPTGTIANXXXXXXXXX 137 GR I +F ++ ++ ++ TL + R ++ G +AN Sbjct: 71 HGR---IDALSFGQIAQLDAGSWHSPAFAGEPVPTLAAVARYLRANGLLANIEIKPVPGT 127 Query: 138 XXXXXXXHSLDMLEQVFLSGCEKERALLAQRRQPQLRKLLNADIR--LFRTLPYEFAVEQ 195 +LD +G + ++ R+ R L TLP ++ Sbjct: 128 EWRTGAALALDARTLWADAGVPPLLSSFSETALAAAREAAPELPRALLLDTLPADWL--- 184 Query: 196 TCREALEA-SCFGINIYHEVVTPWFMERARASGLPVYVWTVNETQ 239 + L A C ++ H +TP + A A+G V +TVN+ + Sbjct: 185 ---DRLRALDCVALDANHRALTPEVIAAAHAAGFRVCCYTVNDVE 226 >STM3553 [C] COG0584 Glycerophosphoryl diester phosphodiesterase Length = 246 Score = 49.3 bits (116), Expect = 7e-06, Method: Compositional matrix adjust. Identities = 31/109 (28%), Positives = 56/109 (51%), Gaps = 3/109 (2%) Query: 22 PFPFITAHTGCME-TLDNTLDAVRMGIELGADIVEDDVRVTKDGIAVMAHDD-IWTTAAG 79 P+P I AH G + +NTL A+ +G G ++E D +++KDG + HDD + T+ G Sbjct: 5 PYPHIVAHRGGGKLAPENTLAAIDVGARYGHTMIEFDAKLSKDGEIFLLHDDNLERTSNG 64 Query: 80 RSISISETNFTELKNVEVAVDYAGKQGSMTFSTLEEMLRLIKPTGTIAN 128 ++ E N+ +L V+ ++G+ L ++ + G +AN Sbjct: 65 WGVA-GELNWQDLLRVDAGGWFSGEFKGEPLPLLSQVAERCRKHGMMAN 112 >lin0625_2 [C] COG0584 Glycerophosphoryl diester phosphodiesterase Length = 243 Score = 48.9 bits (115), Expect = 9e-06, Method: Compositional matrix adjust. Identities = 59/246 (23%), Positives = 106/246 (43%), Gaps = 28/246 (11%) Query: 29 HTG-CMETLDNTLDAVRMGIELGADIVEDDVRVTKDGIAVMAHDDIWTTAAGRSISISET 87 H G M ++NT++A+ + GAD E D++ TKD V+ HD AG S +++ Sbjct: 1 HRGDTMNAVENTVEAIESAAKSGADYSEIDIQETKDHQFVVFHDMTLRRLAGSSKRVADM 60 Query: 88 NFTELKNVEV-AVDYAGKQGSMTFSTLEEMLRLIKPTGTIANXXXXXXXXXXXXXXXXHS 146 EL+ +V + DY S ++ +E+++ K S Sbjct: 61 TLKELQQTKVKSGDY-----SSHIASFDEIIKTAKKN--------KIDLLVEVKLHGGES 107 Query: 147 LDMLEQ-VFLSGCEK--ERALLAQRRQPQLRKLLNADIRLFRTLPYEFAV---EQTCREA 200 DM+E+ V L EK ++ L+ QP + ++ AD L + + +T + Sbjct: 108 SDMVERLVTLLKKEKVTDKYLVQSLNQPVIEEIEKADPSLKTGIILALNIGNLPKTSADF 167 Query: 201 LEASCFGINIYHEVVTPWFMERARASGLPVYVWTVNETQLMEHYADLGVASITARNVQAL 260 + F IN + +A+ + V+VWTVN+ +LM+ Y V I + Sbjct: 168 IVLEDFSIN-------KRLLTQAKQNDKMVFVWTVNKEKLMQMYLRKNVDGIITNYPKKA 220 Query: 261 ADLKQA 266 +L+++ Sbjct: 221 IELRES 226 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.133 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,912,363 Number of extensions: 609527 Number of successful extensions: 1924 Number of sequences better than 1.0e-05: 5 Number of HSP's gapped: 1928 Number of HSP's successfully gapped: 5 Length of query: 271 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 166 Effective length of database: 26,268,361 Effective search space: 4360547926 Effective search space used: 4360547926 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 115 (48.9 bits)