BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0564.0018.p (281 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGl602 [E] COG1176 ABC-type spermidine/putrescine transport syst... 87 4e-17 YPO2031 [E] COG1176 ABC-type spermidine/putrescine transport sys... 86 7e-17 AGl889 [E] COG1176 ABC-type spermidine/putrescine transport syst... 80 3e-15 PA0303 [E] COG1176 ABC-type spermidine/putrescine transport syst... 74 3e-13 SMa2205 [E] COG1176 ABC-type spermidine/putrescine transport sys... 72 1e-12 >AGl602 [E] COG1176 ABC-type spermidine/putrescine transport system, permease component I Length = 530 Score = 86.7 bits (213), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 18/211 (8%), Positives = 43/211 (20%), Gaps = 4/211 (1%) Query: 33 SLKESLTNDGGGYDLSNYKFLFTDKLMRSNIIFTLEITVISSLLVLAISYVLAVYMRFNN 92 + + + + + T+ T+ + Sbjct: 35 RVAMRRVREILRHRFEEGLGHKSIAVRVGAAPSTVRETLRRLERAGLSWPLAEDVSD-AV 93 Query: 93 GFAVRWIRRMYMIPIFIPTVIATYGLMQLLGNHGWAARILYHLGQDSFPRIIYDMKGI-- 150 A + + + L +IL+ Y Sbjct: 94 LEAALYKAAGTKTGHRRIAEPDWAQVHRELKRKHVTLQILWDEYIG-LHPEGYRYSRYCD 152 Query: 151 IIANLWFNIPFTTMLLGSALSSVPDSVIESAKDVGAGRLRIFWRLILPLTYKTLLVAVTF 210 + + +P T +A + V RL R +++F Sbjct: 153 LYRHWALKLPVTMRQDHAAGEKLFVDYAGDTVSVVIDRLSGKTRQAHVFVGVLGASSLSF 212 Query: 211 VFMGVIGSFTAPYLVGPNAPQMLGVSMEQVF 241 G+ A + G + + Sbjct: 213 AHARWSGTLPDWIECHLLALETFGGAPALLV 243 >YPO2031 [E] COG1176 ABC-type spermidine/putrescine transport system, permease component I Length = 288 Score = 85.9 bits (211), Expect = 7e-17, Method: Compositional matrix adjust. Identities = 49/186 (26%), Positives = 106/186 (56%), Gaps = 4/186 (2%) Query: 45 YDLSNYKFLFTDKLMRSNIIFTLEITVISSLLVLAISYVLAVYMRFNNGFAVRWIRRMYM 104 + L ++ + D+++ S + ++++++V+ + + +Y LA+++R A I + Sbjct: 48 FSLEFWRNMLQDEVLHSALFYSVKVSVLGAFGSVIFAYPLALWLR-KPLIAKEGIIAVLR 106 Query: 105 IPIFIPTVIATYGLMQLLGNHGWAARILYHLGQDSFPRIIYDMK---GIIIANLWFNIPF 161 P+FIP ++A + + ++ HG +L LG + P + + G++I +W N+PF Sbjct: 107 APMFIPGMVAAFLFVNIIAYHGVINELLMALGVINEPLRMQNDDFGWGVVILQVWKNLPF 166 Query: 162 TTMLLGSALSSVPDSVIESAKDVGAGRLRIFWRLILPLTYKTLLVAVTFVFMGVIGSFTA 221 +L+G A++++ + V+++A ++GA RLR F +++PLT + V + VF+G +G F Sbjct: 167 ALILVGGAVNAIRNDVLDAASNLGANRLRSFTGIVVPLTLPAVQVTLILVFIGALGDFAF 226 Query: 222 PYLVGP 227 + GP Sbjct: 227 FSVAGP 232 >AGl889 [E] COG1176 ABC-type spermidine/putrescine transport system, permease component I Length = 832 Score = 80.5 bits (197), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 16/201 (7%), Positives = 28/201 (13%), Gaps = 9/201 (4%) Query: 44 GYDLSNYKFLFTDKLMRSNIIFTLE----ITVISSLLVLAISYVLAVYMRFNNGFAVRWI 99 +L L V F A Sbjct: 91 WDNLDFSHITVGVLSQAYEYYLRKHAPKQQQQEGGFYTPHPIANLMVSAAFRGLNAQG-- 148 Query: 100 RRMYMIPIFIPTVIATYGLMQLLGNHGWAARILYHLGQDSFP-RIIYDMKGIIIANLWFN 158 + + + AAR + K + ++ Sbjct: 149 --ISAAARILDPAAGAGVFLLAAFRELVAARWRSEGHRPDTAALREILYKQVRGFDINEA 206 Query: 159 IPFTTMLLGSALSSVPDSVIESAKDVGAGRLRIFWRLILPLTYKTLLVAVTFVFMGVIGS 218 L LS D + + LR T + Sbjct: 207 ALRFCALGLYLLSIELDPNPKPVDKLHFDDLRGVVLHRPVDTTDVDRPEAKQLGSLGSLI 266 Query: 219 FTAPYLVGPNAPQMLGVSMEQ 239 + Sbjct: 267 GEEHDGQYDLVIGNPPWASAT 287 >PA0303 [E] COG1176 ABC-type spermidine/putrescine transport system, permease component I Length = 293 Score = 73.9 bits (180), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 55/204 (26%), Positives = 99/204 (48%), Gaps = 3/204 (1%) Query: 46 DLSNYKFLFTDKLMRSNIIFTLEITVISSLLVLAISYVLAVYMRFNNGFAVRWIRRMYMI 105 +L NY L D L + +L++ IS+L+ L + Y +A + ++ + + + M+ Sbjct: 58 NLGNYLMLTDDPLYIDAYLGSLKMAFISTLICLLVGYPMAYAIARSSQSSQTVLLLLIMM 117 Query: 106 PIFIPTVIATYGLMQLLGNHGWAARILYHLGQDSFPRIIYDMK-GIIIANLWFNIPFTTM 164 P + +I Y M +L N+G +L LG S P I + + I ++ +PF + Sbjct: 118 PTWTAILIRVYAWMGILSNNGLLNSVLMGLGLISEPLTILNTNIAVYIGIVYSYLPFMIL 177 Query: 165 LLGSALSSVPDSVIESAKDVGAGRLRIFWRLILPLTYKTLLVAVTFVFMGVIGSFTAPYL 224 L + L S++E+A D+GA FW++ +PL+ ++ VF+ +G F P L Sbjct: 178 PLYANLVKHDPSLLEAASDLGARNFTSFWKITVPLSKNGIIAGCMLVFIPAVGEFVIPEL 237 Query: 225 VGPNAPQMLGVSMEQVFGVFQDRQ 248 +G M+G + Q F F +R Sbjct: 238 LGGPETLMIGKVLWQEF--FNNRD 259 >SMa2205 [E] COG1176 ABC-type spermidine/putrescine transport system, permease component I Length = 289 Score = 71.6 bits (174), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 46/191 (24%), Positives = 98/191 (51%), Gaps = 2/191 (1%) Query: 47 LSNY-KFLFTDKLMRSNIIFTLEITVISSLLVLAISYVLAVYMRFNNGFAVRWIRRMYMI 105 L NY +FL + + + F++ I ++++ + + +Y +A+Y+ +G + + M Sbjct: 50 LENYTRFLTEPWYLVNTLWFSVRIALLATAISVICAYPVALYIAKTSGLQRNVLMALTMA 109 Query: 106 PIFIPTVIATYGLMQLLGNHGWAARILYHLGQDSFP-RIIYDMKGIIIANLWFNIPFTTM 164 P+ I V YG + + G ++ L P R ++D+KG++I ++ P+ + Sbjct: 110 PLLIGLVTLVYGWIVIFRGGGLMNSLMIALRVYDQPVRYMWDIKGVVILLVYIGTPYIVL 169 Query: 165 LLGSALSSVPDSVIESAKDVGAGRLRIFWRLILPLTYKTLLVAVTFVFMGVIGSFTAPYL 224 L ++ + ++E+A++VGA R FW+++ PL+ L + VF +F P + Sbjct: 170 SLLDSIERINPFLVEAARNVGANRWTAFWKIVFPLSTPGLYAGLVIVFSLNFAAFAVPLM 229 Query: 225 VGPNAPQMLGV 235 +G + QM+G+ Sbjct: 230 IGDSNTQMIGL 240 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.329 0.144 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,379,738 Number of extensions: 683674 Number of successful extensions: 3900 Number of sequences better than 1.0e-05: 165 Number of HSP's gapped: 3881 Number of HSP's successfully gapped: 165 Length of query: 281 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 176 Effective length of database: 26,268,361 Effective search space: 4623231536 Effective search space used: 4623231536 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 115 (48.9 bits)