BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0564.0016.p (343 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMa2207 [E] COG3842 ABC-type spermidine/putrescine transport sys... 290 3e-78 BMEII1123 [E] COG3842 ABC-type spermidine/putrescine transport s... 285 9e-77 mll3065 [E] COG3842 ABC-type spermidine/putrescine transport sys... 283 2e-76 all5044 [E] COG3842 ABC-type spermidine/putrescine transport sys... 281 1e-75 STM1376 [M] COG4238 Murein lipoprotein 278 1e-74 >SMa2207 [E] COG3842 ABC-type spermidine/putrescine transport systems, ATPase components Length = 361 Score = 290 bits (741), Expect = 3e-78, Method: Compositional matrix adjust. Identities = 148/306 (48%), Positives = 205/306 (66%), Gaps = 13/306 (4%) Query: 5 LSIQKLTKRYKTGDGVTDISLDVKKGEMITLLGPSGCGKTTVLRSVGGFLDPDSGDIMIE 64 + ++ +TKRY V ++S DV +GE ++LLGPSGCGKTT LR + GF+DP G + + Sbjct: 6 IQVEGITKRYGAMTAVDNVSFDVGQGEFVSLLGPSGCGKTTTLRMIAGFVDPSGGLLRVR 65 Query: 65 GKSVLSAPPEKRPTSMVFQGYNLWPHMTVYENLAFGLKIR-KIRKAERDKAISEVLELVR 123 G+ V PPEKR VFQ Y LWPHMTV EN+AFGLK+R K R RDK ++E L Sbjct: 66 GRDVTHLPPEKRDLGFVFQNYALWPHMTVAENVAFGLKLRKKNRNFIRDK-VAEALTTTG 124 Query: 124 LPGSEKKYPGQLSGGQQQRIAVARSLLLKPAVLLLDEPFSALDAKLRHEMREELREIQAE 183 L G E + P QLSGGQQQR+A+AR+L L+P VLL+DEP S LD LR MR EL+E+QA Sbjct: 125 LSGYENRLPRQLSGGQQQRVALARALALEPQVLLMDEPLSNLDRALRVTMRRELKELQAR 184 Query: 184 LGLTMVFVTHDQEEALSISDRIVVMNHGQIEQIATPQEIYDEPKSLYVAQFIGKMNFMKG 243 + +T ++VTHDQEEALS+S+R+V+MN GQ+EQ+A P E+Y+ P + +VA F+G NF+ G Sbjct: 185 MKMTTLYVTHDQEEALSMSNRVVIMNKGQVEQVAAPFELYENPATGFVADFVGITNFLGG 244 Query: 244 VITE-EG----IQVGSLRFPNA-----AGLTGEVTVAVRPEDIVLESSAGSGLHGIIKQI 293 ++T EG +++GS + A L +V +RPE + L+++A G + + Q+ Sbjct: 245 IVTSAEGGGVEVKLGSGQMLRAVTKQPVALGTKVRALIRPERVTLKTTAADGDNILTGQV 304 Query: 294 MVLGHY 299 VL Y Sbjct: 305 -VLAEY 309 >BMEII1123 [E] COG3842 ABC-type spermidine/putrescine transport systems, ATPase components Length = 353 Score = 285 bits (728), Expect = 9e-77, Method: Compositional matrix adjust. Identities = 148/306 (48%), Positives = 209/306 (68%), Gaps = 12/306 (3%) Query: 3 AHLSIQKLTKRYKTGDGVTDISLDVKKGEMITLLGPSGCGKTTVLRSVGGFLDPDSGDIM 62 A L+I+ L K + T V D +L V+KGE ++ LGPSGCGKTTVLR + GF PD+G I Sbjct: 2 AFLNIKNLKKSFGTNTVVHDFNLAVEKGEFVSFLGPSGCGKTTVLRMIAGFETPDNGSIE 61 Query: 63 IEGKSVLSAPPEKRPTSMVFQGYNLWPHMTVYENLAFGLKIRKIRKAERDKAISEVLELV 122 I+GK V++ P +R MVFQ Y L+P+MTV +N++FGL++ ++E D + E+L L+ Sbjct: 62 IDGKDVVNLRPNQRNIGMVFQAYALFPNMTVAQNVSFGLRVSGKSRSEIDATVKEMLSLI 121 Query: 123 RLPGSEKKYPGQLSGGQQQRIAVARSLLLKPAVLLLDEPFSALDAKLRHEMREELREIQA 182 RL +YP Q+SGGQQQR+A+AR+L KP VLLLDEP SALDAK+R +REE+R IQ Sbjct: 122 RLDHLADRYPYQMSGGQQQRVALARALATKPQVLLLDEPLSALDAKIRISLREEIRAIQQ 181 Query: 183 ELGLTMVFVTHDQEEALSISDRIVVMNHGQIEQIATPQEIYDEPKSLYVAQFIGKMNFMK 242 +LG+T VFVTHDQEEALSISDRIVVM+ G+ +QI +P +IY+ P S +VA F+G +N ++ Sbjct: 182 KLGITTVFVTHDQEEALSISDRIVVMHEGRADQIGSPFDIYNRPASRFVASFVGTLNMLE 241 Query: 243 GVITE--------EGIQVGSLRFPNAAGLTGE-VTVAVRPEDIVLES--SAGSGLHGIIK 291 ++E +G Q+ +++ A G+ +T+A+RPE + LE+ S + L I Sbjct: 242 ASVSEPAQNSIDLDGRQI-TVQETLAHHPKGKPLTLALRPEAVSLEARKSHDTSLEATID 300 Query: 292 QIMVLG 297 + LG Sbjct: 301 DVHFLG 306 >mll3065 [E] COG3842 ABC-type spermidine/putrescine transport systems, ATPase components Length = 182 Score = 283 bits (725), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 0/341 (0%), Positives = 0/341 (0%), Gaps = 10/341 (2%) Query: 5 LSIQKLTKRYKTGDGVTDISLDVKKGEMITLLGPSGCGKTTVLRSVGGFLDPDSGDIMIE 64 + + + + ++ G Sbjct: 6 VILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKGQLLVTRRALSKKAWPGVWTNS 65 Query: 65 GKSVLSAPPEKRPTSMVFQGYNLWPHMTVYENLAFGLKIRKIRKAERDKAISEVLELVRL 124 + Y L +T E++ + R + + + R Sbjct: 66 VCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDFRYRATDPSGIVENEVCPVFAART 125 Query: 125 PGSEKKYPGQLSGGQQQRIAVARSLLLKPAVLLLDEPFSALDAKLRHEMREELREIQAEL 184 Sbjct: 126 185 Query: 185 GLTMVFVTHDQEEALSISDRIVVMNHGQIEQIATPQEIYDEPKSLYVAQFIGKMNFMKGV 244 Sbjct: 186 245 Query: 245 ITEEG---IQVGSLRFPNAAGLTGE-----VTVAVRPEDIVLESSAG--SGLHGIIKQIM 294 Sbjct: 246 305 Query: 295 VLGHYAEVSADIRNHGVIRAFMARDKVKELQQGQEISVQFS 335 Sbjct: 306 346 >all5044 [E] COG3842 ABC-type spermidine/putrescine transport systems, ATPase components Length = 381 Score = 281 bits (719), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 143/320 (44%), Positives = 204/320 (63%), Gaps = 16/320 (5%) Query: 5 LSIQKLTKRYKTGDGVTDISLDVKKGEMITLLGPSGCGKTTVLRSVGGFLDPDSGDIMIE 64 + ++ + K + V + LDV++GE ++LGPSGCGKTT LR + GF D+G ++I+ Sbjct: 22 VELRNVFKFFNQEPAVHGVDLDVRQGEFFSILGPSGCGKTTTLRLIAGFEQVDAGKLLIQ 81 Query: 65 GKSVLSAPPEKRPTSMVFQGYNLWPHMTVYENLAFGLKIRKIRKAERDKAISEVLELVRL 124 G+ + + PP +RP + VFQ Y L+ H+ V++N+AFGL+++K RK+E + + E L+LV++ Sbjct: 82 GQPMTNIPPYRRPVNTVFQSYALFNHLNVWDNVAFGLRLKKSRKSEVESRVKEALKLVKM 141 Query: 125 PGSEKKYPGQLSGGQQQRIAVARSLLLKPAVLLLDEPFSALDAKLRHEMREELREIQAEL 184 ++P QLSGGQQQR+A+AR+L+ +PAV+LLDEP ALD KLR EM+ EL + L Sbjct: 142 ESLRSRFPSQLSGGQQQRVALARALVNRPAVVLLDEPLGALDLKLRKEMQVELSNLHKNL 201 Query: 185 GLTMVFVTHDQEEALSISDRIVVMNHGQIEQIATPQEIYDEPKSLYVAQFIGKMNFMKG- 243 GLT V VTHDQEEALS+SDRI VMN G+IEQI TPQEIY+ PK+ +VA FIG N G Sbjct: 202 GLTFVMVTHDQEEALSLSDRIAVMNQGKIEQIGTPQEIYERPKTSFVADFIGDTNLFSGE 261 Query: 244 ----------VITEEGIQVGSLRFPNA-AGLTGEVTVAVRPEDIVLE----SSAGSGLHG 288 ++T+ G+ + R + A L V V+VRPE I L SS + G Sbjct: 262 ITVLEAEYIQIVTKTGLTIVVARNEDTPAELLKPVVVSVRPEKIQLSLYPPSSLNNCFEG 321 Query: 289 IIKQIMVLGHYAEVSADIRN 308 + +M LG + + N Sbjct: 322 RLINVMYLGTHVNYVVQLIN 341 >STM1376 [M] COG4238 Murein lipoprotein Length = 79 Score = 278 bits (711), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 0/310 (0%), Positives = 0/310 (0%), Gaps = 16/310 (5%) Query: 5 LSIQKLTKRYKTGDGVTDISLDVKKGEMITLLGPSGCGKTTVLRSVGGFLDPDSGDIMIE 64 I T + + + + + + + + D Sbjct: 6 QLILGAVVLGSTLLAGCSSNAKIDQLSSDVQTLSAKVEQLSNDVNAMRSDVQAAKDDAAR 65 Query: 65 GKSVLSAPPEKRPTSMVFQGYNLWPHMTVYENLAFGLKIRKIRKAERDKAISEVLELVRL 124 Sbjct: 66 125 Query: 125 PGSEKKYPGQLSGGQQQRIAVARSLLLKPAVLLLDEPFSALDAKLRHEMREELREIQAEL 184 Sbjct: 126 185 Query: 185 GLTMVFVTHDQEEALSISDRIVVMNHGQIEQIATPQEIYDEPKSLYVAQFIGKMNFMKGV 244 Sbjct: 186 245 Query: 245 ITE--------EGIQVGSLR-FPNAAGLTGE-VTVAVRPEDIVL------ESSAGSGLHG 288 Sbjct: 246 305 Query: 289 IIKQIMVLGH 298 Sbjct: 306 315 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,306,043 Number of extensions: 1027766 Number of successful extensions: 9760 Number of sequences better than 1.0e-05: 2192 Number of HSP's gapped: 6570 Number of HSP's successfully gapped: 2447 Length of query: 343 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 236 Effective length of database: 26,037,693 Effective search space: 6144895548 Effective search space used: 6144895548 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)