BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0564.0015.p (274 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value YPO2030 [E] COG1177 ABC-type spermidine/putrescine transport sys... 99 6e-21 SMa1751 [E] COG1177 ABC-type spermidine/putrescine transport sys... 88 2e-17 BMEII0920 [E] COG1177 ABC-type spermidine/putrescine transport s... 86 7e-17 AGl601 [E] COG1177 ABC-type spermidine/putrescine transport syst... 82 1e-15 SMa0802 [E] COG1177 ABC-type spermidine/putrescine transport sys... 81 1e-15 >YPO2030 [E] COG1177 ABC-type spermidine/putrescine transport system, permease component II Length = 278 Score = 99.4 bits (246), Expect = 6e-21, Method: Compositional matrix adjust. Identities = 58/191 (30%), Positives = 95/191 (49%) Query: 42 WSYPDPLPREWSLKWWSFVLSRDGIAESIGLSFGIAAIVTAVSILLCVPAAYAFARIXXX 101 WSYPD P+ S W V + ++E++ S+ IA +V A S+LL +P AYAF R+ Sbjct: 45 WSYPDIFPQVLSFNRWLLVWEKTSLSEALINSYTIAPVVAATSLLLSLPTAYAFGRMRFR 104 Query: 102 XXXXXXXXXXXTHAFPKMGLYVSIAVLFYKIGLMNTLLGVVLIHIINVLMYMTWIPTASF 161 P M + + + + + + N +VL H + L Y I +A F Sbjct: 105 GKAVAEMLTLIPLVMPGMIIGIFFSAMLMNLNISNPFFSIVLGHTVLTLPYSIRIMSAGF 164 Query: 162 RNVHMAQEESARDVGASPFRVFRSITLPLAMPGIIVASIFTFLSSLDEAQGTYLVGVPNF 221 + V +++RD+GA + F + LPL P + A IF + SL+E Y++G P+F Sbjct: 165 KAVPQDLIDASRDLGAGIWGTFCNAYLPLLKPSFLAALIFCLVKSLEEFSIAYVLGAPDF 224 Query: 222 RTMPVVMYGII 232 T+P ++Y + Sbjct: 225 ITVPTILYSFL 235 >SMa1751 [E] COG1177 ABC-type spermidine/putrescine transport system, permease component II Length = 260 Score = 87.8 bits (216), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 62/225 (27%), Positives = 105/225 (46%), Gaps = 1/225 (0%) Query: 10 PKKIIEFLLLAAFVIFFMGPLLNLAILAFTGNWSYPDPLPREWSLKWWSFVLSRDGIAES 69 P K+ +F + ++F + PL+ + LAFT + P+P WSL+W+ + + D + Sbjct: 2 PMKLAKFAFASLTILFLVAPLIAILPLAFTSSVILTYPIP-SWSLRWFEELFTADAWRRA 60 Query: 70 IGLSFGIAAIVTAVSILLCVPAAYAFARIXXXXXXXXXXXXXXTHAFPKMGLYVSIAVLF 129 I S I T ++ +L A+ P + L V + VL Sbjct: 61 IFNSLIIGTGTTLLATILGTAASLGLRNRLIFFRGSMRTLFLLPMVVPAVVLGVGMQVLL 120 Query: 130 YKIGLMNTLLGVVLIHIINVLMYMTWIPTASFRNVHMAQEESARDVGASPFRVFRSITLP 189 GL N+ GV++ H + + ++ + + E +A+ +GASP VF +TLP Sbjct: 121 AGFGLTNSYAGVIIAHTVVAVPFVVVSVIGALDGIDERVELAAQSLGASPATVFHRVTLP 180 Query: 190 LAMPGIIVASIFTFLSSLDEAQGTYLVGVPNFRTMPVVMYGIITD 234 LA+PG++ ++ F +SLDE T V PN RT+ M+ I + Sbjct: 181 LALPGVLSGAVLAFATSLDEVVLTLFVAGPNQRTLARQMFSSIRE 225 >BMEII0920 [E] COG1177 ABC-type spermidine/putrescine transport system, permease component II Length = 258 Score = 85.9 bits (211), Expect = 7e-17, Method: Compositional matrix adjust. Identities = 64/229 (27%), Positives = 106/229 (46%), Gaps = 13/229 (5%) Query: 12 KIIEFLLLAAFVIFFMGPLLNLAILAFTGN--WSYPDPLPREWSLKWWSFVLS----RDG 65 ++ + L A V+F + PL + LAFT + +YP P WS++W+ + + R Sbjct: 3 RVAKILFGVAAVVFLVAPLFAILPLAFTSSVFLTYPVP---GWSVRWFEELTTAEVWRRA 59 Query: 66 IAESIGLSFGIAAIVTAVSILLCVPAAYAFARIXXXXXXXXXXXXXXTHAFPKMGLYVSI 125 I S+ + FG A+ T + + AA P + L V + Sbjct: 60 IVNSLIIGFGTMALATVLGTM----AALGLRTRGLFLSGPIRTLFLLPMVVPAVVLGVGM 115 Query: 126 AVLFYKIGLMNTLLGVVLIHIINVLMYMTWIPTASFRNVHMAQEESARDVGASPFRVFRS 185 + F ++ + N+ GV++ H + + ++ + + + E +A +GA+P VFR Sbjct: 116 QLFFVRLDIANSYFGVIVAHTVVSIPFVLVSVSGALAGIDRRLELAAESLGAAPLTVFRK 175 Query: 186 ITLPLAMPGIIVASIFTFLSSLDEAQGTYLVGVPNFRTMPVVMYGIITD 234 ITLPLAMPGI + F +SLDE T V PN RT+ M+ I + Sbjct: 176 ITLPLAMPGIASGGVLAFATSLDEVVLTLFVAGPNQRTLARQMFSTIRE 224 >AGl601 [E] COG1177 ABC-type spermidine/putrescine transport system, permease component II Length = 530 Score = 81.6 bits (200), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 14/195 (7%), Positives = 34/195 (17%) Query: 41 NWSYPDPLPREWSLKWWSFVLSRDGIAESIGLSFGIAAIVTAVSILLCVPAAYAFARIXX 100 + + L R A + + L R Sbjct: 53 LGHKSIAVRVGAAPSTVRETLRRLERAGLSWPLAEDVSDAVLEAALYKAAGTKTGHRRIA 112 Query: 101 XXXXXXXXXXXXTHAFPKMGLYVSIAVLFYKIGLMNTLLGVVLIHIINVLMYMTWIPTAS 160 L+ L + + + + + + M A Sbjct: 113 EPDWAQVHRELKRKHVTLQILWDEYIGLHPEGYRYSRYCDLYRHWALKLPVTMRQDHAAG 172 Query: 161 FRNVHMAQEESARDVGASPFRVFRSITLPLAMPGIIVASIFTFLSSLDEAQGTYLVGVPN 220 + ++ V R + + + G S S + Sbjct: 173 EKLFVDYAGDTVSVVIDRLSGKTRQAHVFVGVLGASSLSFAHARWSGTLPDWIECHLLAL 232 Query: 221 FRTMPVVMYGIITDY 235 + + Sbjct: 233 ETFGGAPALLVPDNA 247 >SMa0802 [E] COG1177 ABC-type spermidine/putrescine transport system, permease component II Length = 266 Score = 81.3 bits (199), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 61/206 (29%), Positives = 100/206 (48%), Gaps = 5/206 (2%) Query: 29 PLLNLAILAF-TGNWS-YPDPLPREWSLKWWSFVLSRDGIAESIGLSFGIAAIVTAVSIL 86 P+L +A+L+F + W +P P W+L+W+ +L +S SF IA IVT +S+L Sbjct: 29 PILFIAVLSFGSSQWLIFPPP---GWTLRWYQELLEDPRWLDSAWTSFRIAIIVTVLSVL 85 Query: 87 LCVPAAYAFARIXXXXXXXXXXXXXXTHAFPKMGLYVSIAVLFYKIGLMNTLLGVVLIHI 146 L + ++ R P + L V++ F +IGL TL G V+ H+ Sbjct: 86 LGLVTSFGLNRGRFLFREALKALFLTPMILPVVVLAVALYAFFLQIGLNGTLTGFVISHL 145 Query: 147 INVLMYMTWIPTASFRNVHMAQEESARDVGASPFRVFRSITLPLAMPGIIVASIFTFLSS 206 + L + T + + E++A GASPF +TLP G+ A+IF+FL+S Sbjct: 146 VLALPFSILSITNALEGFDKSIEDAAVLCGASPFEAKIRVTLPAISHGVFSAAIFSFLTS 205 Query: 207 LDEAQGTYLVGVPNFRTMPVVMYGII 232 DE + P +T+PV ++ + Sbjct: 206 WDEVVVAIFMASPTLQTLPVKVWSTL 231 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.329 0.141 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,487,595 Number of extensions: 691184 Number of successful extensions: 2584 Number of sequences better than 1.0e-05: 140 Number of HSP's gapped: 2546 Number of HSP's successfully gapped: 141 Length of query: 274 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 169 Effective length of database: 26,268,361 Effective search space: 4439353009 Effective search space used: 4439353009 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 115 (48.9 bits)