BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0563.0008.p (364 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yccC [EJ] COG0252 L-asparaginase/archaeal Glu-tRNAGln amidotr... 151 1e-36 STM3598 [EJ] COG0252 L-asparaginase/archaeal Glu-tRNAGln amidotr... 131 1e-30 YPO1386 [EJ] COG0252 L-asparaginase/archaeal Glu-tRNAGln amidotr... 125 9e-29 ZansB [EJ] COG0252 L-asparaginase/archaeal Glu-tRNAGln amidotran... 125 9e-29 ECs3833 [EJ] COG0252 L-asparaginase/archaeal Glu-tRNAGln amidotr... 125 9e-29 >BS_yccC [EJ] COG0252 L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D Length = 375 Score = 151 bits (382), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 114/335 (34%), Positives = 171/335 (51%), Gaps = 16/335 (4%) Query: 27 LPKIQVIATGGTIAGQSVDETSFQNYKAGTLLIEDMVKALPNKDQIAEVSSYQFGNAXXX 86 LP I+++ATGGTIAG +TS YKAG + +E +++A+P IA VS Q N Sbjct: 50 LPNIRILATGGTIAGADQSKTSTTEYKAGVVGVESLIEAVPEMKDIANVSGEQIVNVGST 109 Query: 87 XXXXXXXXXXXLKVDEALKTQXX--XXXXXXXXXMEEIAYFLDLTVRSPKPVVITGSMRP 144 +++ L + +EE AYFL+LTV+S KPVVI GSMRP Sbjct: 110 NIDNKILLKLAKRINHLLASDDVDGIVVTHGTDTLEETAYFLNLTVKSDKPVVIVGSMRP 169 Query: 145 WTVIGSDAQANLYNAIKLAASGKTKYFGTVLMLNDEFHTAREVTKSNAYRTDTFETPKLG 204 T I +D +NLYNA+K+A + + K GT+++LND +AR VTK+N TDTF++ ++G Sbjct: 170 STAISADGPSNLYNAVKVAGAPEAKGKGTLVVLNDRIASARYVTKTNTTTTDTFKSEEMG 229 Query: 205 MLGYIDEKNIRIYRSNARSLMSEDKWATPFDLSKISPSSLAKVEIAYSYQDAGAGAITGF 264 +G I + IY +N + + T F +S + L +V+I Y YQ+ G+ Sbjct: 230 FVGTIADD---IYFNN--EITRKHTKDTDFSVSNL--DELPQVDIIYGYQNDGSYLFDAA 282 Query: 265 VEDXXXXXXXXXXXXXXXSKAMGEARTKAIKEKGVIFVATTRTGSG-----SNYTSADGI 319 V+ S A + A+K KGV V +TRTG+G +Y D + Sbjct: 283 VKAGAKGIVFAGSGNGSLSDAAEKGADSAVK-KGVTVVRSTRTGNGVVTPNQDYAEKD-L 340 Query: 320 IAGDNLNAAHARIXXXXXXXXXXDFNTIKGWFDTY 354 +A ++LN AR+ D I+ +F+ Y Sbjct: 341 LASNSLNPQKARMLLMLALTKTNDPQKIQAYFNEY 375 >STM3598 [EJ] COG0252 L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D Length = 347 Score = 131 bits (330), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 106/347 (30%), Positives = 157/347 (45%), Gaps = 19/347 (5%) Query: 14 VQGTVVSTQSPVKLPKIQVIATGGTIAGQSVDETSFQNYKAGTLLIEDMVKALPNKDQIA 73 + V ST S LP I ++ATGGTIAG + + T YK+G L ++ ++ A+P + IA Sbjct: 14 ISHCVFSTTS---LPHIVILATGGTIAGTAANNTQTAGYKSGELGVQTLINAVPEMNNIA 70 Query: 74 EVSSYQFGNAXXXXXXXXXXXXXXLKVDEALKTQXX--XXXXXXXXXMEEIAYFLDLTVR 131 V Q N KV+ L ++E AYFL+LTV+ Sbjct: 71 RVDGEQVANIGSENMTSDIILKLSQKVNALLARDDVDGVVITHGTDTLDETAYFLNLTVK 130 Query: 132 SPKPVVITGSMRPWTVIGSDAQANLYNAIKLAASGKTKYFGTVLMLNDEFHTAREVTKSN 191 S KPVV T +MRP + I +D NL A+ +AA K G +++LND +AR VTK+N Sbjct: 131 SDKPVVFTAAMRPASAISADGAMNLLEAVTVAADPNAKGRGVMVVLNDRIGSARFVTKTN 190 Query: 192 AYRTDTFETPKLGMLGYIDEKNIRIYRSNARSLMSEDKWATPFDLSKISPSSLAKVEIAY 251 A DTF+ P+ G LG I + + + + + + FD+ I L V I Y Sbjct: 191 ATTLDTFKAPEEGYLGVIVNGQPQ-FETRVEKIHT---LRSVFDVRNI--KKLPNVVIIY 244 Query: 252 SYQDAGAGAITGFVEDXXXXXXXXXXXXXXXSKAMGEARTKAIKEKGVIFVATTRTGSGS 311 YQD + S +A K ++ G+I V +RTG+G Sbjct: 245 GYQDDPEYMYDAAIAHHADGIIYAGTGAGSVS-VRSDAGIKKAEKAGIIVVRASRTGNG- 302 Query: 312 NYTSAD----GIIAGDNLNAAHARIXXXXXXXXXXDFNTIKGWFDTY 354 D G+++ D+LN A AR+ + I+ +F TY Sbjct: 303 -VVPLDKGQPGLVS-DSLNPAKARVLLMTALTQTRNPELIQSYFSTY 347 >YPO1386 [EJ] COG0252 L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D Length = 345 Score = 125 bits (315), Expect = 9e-29, Method: Compositional matrix adjust. Identities = 110/355 (30%), Positives = 156/355 (43%), Gaps = 20/355 (5%) Query: 8 YAAEVAVQGTVVSTQSPV-KLPKIQVIATGGTIAGQSVDETSFQNYKAGTLLIEDMVKAL 66 Y + G V SPV LP I ++ATGGTIA D + NY AG L ++ +V+A+ Sbjct: 3 YIKLTVLAGIFVGISSPVFALPNITLLATGGTIA-GGGDSATKSNYTAGKLGVDALVEAV 61 Query: 67 PNKDQIAEVSSYQFGNAXXXXXXXXXXXXXXLKVDEALKTQXXXXXXXXXXXMEEIAYFL 126 P IA + Q N K+++ +EE AYFL Sbjct: 62 PAIKDIANIQGEQVVNIGSQDMNDDVWLTLAKKINKDCTKTDGFVITHGTDTLEETAYFL 121 Query: 127 DLTVRSPKPVVITGSMRPWTVIGSDAQANLYNAIKLAASGKTKYFGTVLMLNDEFHTARE 186 DLTV KPVVI G+MRP T +G+D NLYNA+ +A+ + G ++ +ND T R+ Sbjct: 122 DLTVNCDKPVVIVGAMRPATALGADGPLNLYNAVVVASDADSAKRGVLVAMNDMVFTGRD 181 Query: 187 VTKSNAYRTDTFETPKLGMLGYI-DEKNIRIYRSNARSLMSEDKWATPFDLSKISPSSLA 245 V K+N TF++P G LGYI D K +++ AR L FD+SK+ ++L Sbjct: 182 VVKTNTTSVQTFQSPNTGPLGYIYDGKVNYLHQPAARQLA--------FDISKL--NTLP 231 Query: 246 KVEIAYSYQDAGAGAITGFVEDXXXXXXXXXXXXXXXSKAMGEARTKAIKEKGVIFVATT 305 KV I Y+Y +A + D + + A GV V ++ Sbjct: 232 KVGIIYNYANASDIPAKALIADGYQGIVSAGVGNGNLYHTVFDTLATA-ASHGVAVVRSS 290 Query: 306 RTGSGSNYTSAD------GIIAGDNLNAAHARIXXXXXXXXXXDFNTIKGWFDTY 354 R SGS A+ G +A LN AR+ I+ F TY Sbjct: 291 RVPSGSTTEGAEIDDAKYGFVAAGALNPQKARVLLQLALTQTQKPQEIQKLFHTY 345 >ZansB [EJ] COG0252 L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D Length = 348 Score = 125 bits (315), Expect = 9e-29, Method: Compositional matrix adjust. Identities = 106/334 (31%), Positives = 150/334 (44%), Gaps = 14/334 (4%) Query: 27 LPKIQVIATGGTIAGQSVDETSFQNYKAGTLLIEDMVKALPNKDQIAEVSSYQFGNAXXX 86 LP I ++ATGGTIA D + NY AG + +E++V A+P IA V Q N Sbjct: 23 LPNITILATGGTIA-GGGDSATKSNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQ 81 Query: 87 XXXXXXXXXXXLKVDEALKTQXXXXXXXXXXXMEEIAYFLDLTVRSPKPVVITGSMRPWT 146 K++ MEE AYFLDLTV+ KPVV+ G+MRP T Sbjct: 82 DMNDDVWLTLAKKINTDCDKTDGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPST 141 Query: 147 VIGSDAQANLYNAIKLAASGKTKYFGTVLMLNDEFHTAREVTKSNAYRTDTFETPKLGML 206 + +D NLYNA+ AA + G ++++ND R+VTK+N TF++ G L Sbjct: 142 SMSADGPFNLYNAVVTAADKASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPL 201 Query: 207 GYIDEKNIRIYRSNARSLMSEDKWATPFDLSKISPSSLAKVEIAYSYQDAGAGAITGFVE 266 GYI I R+ AR S+ TPFD+SK+ + L KV I Y+Y +A V+ Sbjct: 202 GYIHNGKIDYQRTPARKHTSD----TPFDVSKL--NELPKVGIVYNYANASDLPAKALVD 255 Query: 267 DXXXXXXXXXXXXXXXSKAMGEARTKAIKEKGVIFVATTRTGSGSNYTSAD------GII 320 K++ + A K G V ++R +G+ A+ G I Sbjct: 256 AGYDGIVSAGVGNGNLYKSVFDTLATAAKN-GTAVVRSSRVPTGATTQDAEVDDAKYGFI 314 Query: 321 AGDNLNAAHARIXXXXXXXXXXDFNTIKGWFDTY 354 A LN AR+ D I+ F+ Y Sbjct: 315 ASGTLNPQKARVLLQLALTQTKDPQQIQQIFNQY 348 >ECs3833 [EJ] COG0252 L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D Length = 348 Score = 125 bits (315), Expect = 9e-29, Method: Compositional matrix adjust. Identities = 106/334 (31%), Positives = 150/334 (44%), Gaps = 14/334 (4%) Query: 27 LPKIQVIATGGTIAGQSVDETSFQNYKAGTLLIEDMVKALPNKDQIAEVSSYQFGNAXXX 86 LP I ++ATGGTIA D + NY AG + +E++V A+P IA V Q N Sbjct: 23 LPNITILATGGTIA-GGGDSATKSNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQ 81 Query: 87 XXXXXXXXXXXLKVDEALKTQXXXXXXXXXXXMEEIAYFLDLTVRSPKPVVITGSMRPWT 146 K++ MEE AYFLDLTV+ KPVV+ G+MRP T Sbjct: 82 DMNDDVWLTLAKKINTDCDKTDGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPST 141 Query: 147 VIGSDAQANLYNAIKLAASGKTKYFGTVLMLNDEFHTAREVTKSNAYRTDTFETPKLGML 206 + +D NLYNA+ AA + G ++++ND R+VTK+N TF++ G L Sbjct: 142 SMSADGPFNLYNAVVTAADKASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPL 201 Query: 207 GYIDEKNIRIYRSNARSLMSEDKWATPFDLSKISPSSLAKVEIAYSYQDAGAGAITGFVE 266 GYI I R+ AR S+ TPFD+SK+ + L KV I Y+Y +A V+ Sbjct: 202 GYIHNGKIDYQRTPARKHTSD----TPFDVSKL--NELPKVGIVYNYANASDLPAKALVD 255 Query: 267 DXXXXXXXXXXXXXXXSKAMGEARTKAIKEKGVIFVATTRTGSGSNYTSAD------GII 320 K++ + A K G V ++R +G+ A+ G I Sbjct: 256 AGYDGIVSAGVGNGNLYKSVFDTLATAAKN-GTAVVRSSRVPTGATTQDAEVDDAKYGFI 314 Query: 321 AGDNLNAAHARIXXXXXXXXXXDFNTIKGWFDTY 354 A LN AR+ D I+ F+ Y Sbjct: 315 ASGTLNPQKARVLLQLALTQTKDPQQIQQIFNQY 348 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.129 0.360 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,598,454 Number of extensions: 786966 Number of successful extensions: 881 Number of sequences better than 1.0e-05: 19 Number of HSP's gapped: 854 Number of HSP's successfully gapped: 19 Length of query: 364 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 256 Effective length of database: 25,922,359 Effective search space: 6636123904 Effective search space used: 6636123904 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 116 (49.3 bits)