BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0563.0001.p (306 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA2101 [GER] COG0697 Permeases of the drug/metabolite transporte... 82 7e-16 YPO1203 [GER] COG0697 Permeases of the drug/metabolite transport... 65 2e-10 STM1862 [GER] COG0697 Permeases of the drug/metabolite transport... 64 2e-10 AF0266 [GER] COG0697 Permeases of the drug/metabolite transporte... 54 3e-07 TVN0748 [GER] COG0697 Permeases of the drug/metabolite transport... 52 8e-07 >PA2101 [GER] COG0697 Permeases of the drug/metabolite transporter (DMT) superfamily Length = 296 Score = 82.4 bits (202), Expect = 7e-16, Method: Compositional matrix adjust. Identities = 76/243 (31%), Positives = 125/243 (51%), Gaps = 19/243 (7%) Query: 6 YSLVCLIFGTTFLAIKIGVDAGTPPFFSAGLRFFVAGAVLFLFMAWRGKASFRLLLRKEM 65 + +VC+ +GTT+L IKI V++ PP +AGLRF +A + LF R + F + + Sbjct: 10 FLVVCISWGTTWLGIKIAVES-VPPLTAAGLRFLIAFPLFLLFARLRHETIF---FPRRL 65 Query: 66 LFTGAALTFGTFSALYW----AEQYVSSGLAAVLSATGPMMILLMQTAFLRQKAPSYSLL 121 L +T G FS Y+ EQYV+SGL A+L ++ P+ IL+ FLR++ +L Sbjct: 66 LAFFVFVTLGYFSVPYYLLNYGEQYVASGLTALLFSSMPVFILIFSAIFLRERIYLSQIL 125 Query: 122 GCIIGFTGVLLLVLPSLAIDVSPLWIAGCAIVLTGEICYAAGAIYSKKVTRTFSGESPVA 181 G IGF G L +++ S + + + G +L I +A + +K R + + Sbjct: 126 GIAIGF-GSLFMIIRSQGLHLDYTELLGVLAILLAAIMHALSYVITK---RQGADIGVIT 181 Query: 182 LNAAQMIYGGALLFVLSLFTENVHPGFLLSFETAGS---LLYLTVVGSMVGHTLFYWLVA 238 N + G LF+ L E HP LS T+ S L+YL +V S+ G ++++L+ Sbjct: 182 FNTLPIGIAGLGLFMAGLVME--HPN--LSAVTSRSWAALIYLGLVASVGGFIVYFFLLK 237 Query: 239 KTN 241 + + Sbjct: 238 RLS 240 >YPO1203 [GER] COG0697 Permeases of the drug/metabolite transporter (DMT) superfamily Length = 296 Score = 64.7 bits (156), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 71/245 (28%), Positives = 116/245 (47%), Gaps = 16/245 (6%) Query: 12 IFGTTFLAIKIGVDAGTPPFFSAGLRFFVAGAVLFLFMAWRGKASFRLLLRKEMLFTGA- 70 ++G+T+L I+IGV++ PP AGLR+ +AG +LF F+A RG A L G Sbjct: 22 VWGSTYLVIRIGVES-WPPLMMAGLRYLIAGVLLFSFLAIRGHALPTLRQWAAASAIGIL 80 Query: 71 ALTFGTFSALYWAEQYVSSGLAAVLSATGPMMILLMQTAF-LRQKAPSYSLLGCIIGFTG 129 L G Q+V SG+AAV+ AT P+ L + +R ++ G +G G Sbjct: 81 LLAIGNGLVTIAEHQHVPSGIAAVMVATVPLFTLCFSLLWGMRNTKLEWA--GIALGLVG 138 Query: 130 VLLLVLPSLAIDVSPLWIAGCAIVLTGEICYAAGAIYSKKVTRTFSGESPVALNAAQMIY 189 ++LL S + +P+ G ++L +A G+++S ++ S AAQM+ Sbjct: 139 IILLNTGSNLLG-NPV---GAMLILLASASWAFGSVWSSRLALPAGAMS----GAAQMLV 190 Query: 190 GGALLFVLSLFT-ENVHPGFLLSFETAGSLLYLTVVGSMVGHTLFYWLVAKTNXXXXXXX 248 G +L + S + E + +S A LLYL V GSM+ + + +L+ Sbjct: 191 AGVVLLLASTLSGEELQQPPTMSGILA--LLYLIVFGSMLAISAYMFLLKNVRPAVATSY 248 Query: 249 XXXHP 253 +P Sbjct: 249 AYVNP 253 >STM1862 [GER] COG0697 Permeases of the drug/metabolite transporter (DMT) superfamily Length = 304 Score = 64.3 bits (155), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 68/243 (27%), Positives = 111/243 (45%), Gaps = 11/243 (4%) Query: 2 IMLAYSLVCLIFGTTFLAIKIGVDAGTPPFFSAGLRFFVAGAVLFLFMAWRGKASFRLLL 61 I + + LV L +GTT+LA++I V+ PP F+ G+RF A L + +AW K + L Sbjct: 6 ISILFMLVSLTWGTTWLAMRIAVET-IPPVFATGMRFMFAAPFLII-IAWLRKKTL-LFP 62 Query: 62 RKEMLFTGAALTFG---TFSALYWAEQYVSSGLAAVLSATGPMMILLMQTAFLRQKAPSY 118 + LF F FS + + E YV+SGLAA++ A P+ +L+ FL +KA Sbjct: 63 PGQRLFQFVICIFYFCIPFSLMIYGETYVNSGLAAIIFANMPVAVLIASVLFLNEKAKLM 122 Query: 119 SLLGCIIGFTGVLLLVLPSLAIDVSPLWIAGCAIVLTGEICYAAGAIYSKKVTRTFSGES 178 + G I T + ++L W G +++ + +A IY++ R+ + S Sbjct: 123 QIAGLTIAITALTGILLEETNTSTESHW-QGITALISAVLIHA--IIYTQCKKRSCT-VS 178 Query: 179 PVALNAAQMIYGGALLFVLSLFTENVHPGFLLSFETAGSLLYLTVVGSMVGHTLFYWLVA 238 + NA + G +L F E S + + LYL + G ++ L Sbjct: 179 VITFNALPCLLAGLILSATGWFFERPQVS-TFSVHSILATLYLGAFAGVFGILCYFALQQ 237 Query: 239 KTN 241 K N Sbjct: 238 KAN 240 >AF0266 [GER] COG0697 Permeases of the drug/metabolite transporter (DMT) superfamily Length = 276 Score = 53.9 bits (128), Expect = 3e-07, Method: Compositional matrix adjust. Identities = 59/235 (25%), Positives = 105/235 (44%), Gaps = 15/235 (6%) Query: 7 SLVCLIFGTTFLAIKIGVDAGTPPFFSAGLRFFVAGAVLFLFMAWRGKASFRLLLRKEML 66 +L +G F AIK V P A LRF +A + + + + ++ L + L Sbjct: 8 ALTVTFWGLAFTAIKYSVRF-LSPIAIASLRFAIANTLFAVIIILGKRIKWKDLPKVFAL 66 Query: 67 FTGAALTFGTFSALYWAEQYVSSGLAAVLSATGPMMILLMQTAFLRQKAPSYSLLGCIIG 126 + F L E Y+SSG+A+V+ + P+ +L++ FLR++ ++G II Sbjct: 67 GIFGVSVYHVF--LNLGEVYISSGVASVVISLAPIFVLILSAIFLRERITYSKVVGIIIA 124 Query: 127 FTGVLLLVLPSLAIDVSPLWIAGCAIVLTGEICYAAGAIYSKKVTRTFSGESPVALNAAQ 186 F GV+++ PS A I G A+V+ + A AIY+ S +P+ L + Sbjct: 125 FLGVVVISEPSYA------NIYGIALVMVSTV---AAAIYTTFGKSLLSKYNPITLTSNA 175 Query: 187 MIYGGALLFVLSLFTENVHPGFLLSFETAGSLLYLTVVGSMVGHTLFYWLVAKTN 241 M+ G L+ F + GS+++L + + G+ +Y+ + K Sbjct: 176 MVLGSIPLYP---FLPDSIRSLGGDLNLIGSIVFLGIFSTFFGYLGWYYFLEKEE 227 >TVN0748 [GER] COG0697 Permeases of the drug/metabolite transporter (DMT) superfamily Length = 280 Score = 52.4 bits (124), Expect = 8e-07, Method: Compositional matrix adjust. Identities = 47/179 (26%), Positives = 86/179 (48%), Gaps = 21/179 (11%) Query: 21 KIGVDAGTPPFFSAGLRFFVAGAVLFLFMAWRGKASFRLLLRKEMLFTGAALTFGTFSAL 80 K GV+ +P F G+R+ ++G +L F A + + + ++L M T A Sbjct: 22 KNGVEQVSPSLF-MGIRYLLSGLILLPF-AKKLRITKNIILLAIMTSTSTA--------- 70 Query: 81 YWAEQ--YVSSGLAAVLSATGPMMILLMQTAFLRQKAPSYSLLGCIIGFTGVLLLVLPSL 138 +WA YVS +AVLS + P+ L + + ++ ++G +IGFTGV++ +P + Sbjct: 71 FWAYGLLYVSPAESAVLSYSMPIFSLPIAFLMVSERPSKVEIIGIVIGFTGVIIYGIPLM 130 Query: 139 AIDVSPLWIAGCAIVLTGEICYAAGAIYSKKVTRTFSGESPVALNAAQMIYGGALLFVL 197 I G + ++ + +A+ ++ +K+ E P + NA Q + G LF L Sbjct: 131 ----KGFTIFGAVLTISNAVFWASFTVFYRKLKE----EDPFSTNAMQFLIGSLFLFAL 181 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.330 0.141 0.442 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,216,251 Number of extensions: 698649 Number of successful extensions: 2004 Number of sequences better than 1.0e-05: 34 Number of HSP's gapped: 1998 Number of HSP's successfully gapped: 34 Length of query: 306 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 200 Effective length of database: 26,153,027 Effective search space: 5230605400 Effective search space used: 5230605400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 115 (48.9 bits)