BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0562.0014.p (288 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ppsE_2 [Q] COG3319 Thioesterase domains of type I polyketide ... 488 e-138 BS_ppsA_1 [Q] COG1020 Non-ribosomal peptide synthetase modules a... 488 e-138 BS_ppsA_2 [Q] COG1020 Non-ribosomal peptide synthetase modules a... 488 e-138 BS_pps [G] COG0574 Phosphoenolpyruvate synthase/pyruvate phospha... 488 e-138 CAC0534 [G] COG0574 Phosphoenolpyruvate synthase/pyruvate phosph... 436 e-122 >BS_ppsE_2 [Q] COG3319 Thioesterase domains of type I polyketide synthases or non-ribosomal peptide synthetases Length = 211 Score = 488 bits (1256), Expect = e-138, Method: Compositional matrix adjust. Identities = 0/284 (0%), Positives = 0/284 (0%) Query: 4 MNASRWINENMNEWLGEKNAADTLSQSVRDNVTSEMGLELMDVADVIRPYPEVIDYLQYV 63 Sbjct: 491 550 Query: 64 KADNFLDELHKFDGGQETRDAIYAFLQKYGMHCTGEIDITRTRWSEKPSMLVPMILGNIR 123 Sbjct: 551 610 Query: 124 NFEPHAGGLKFEHGRQEAMAKEQELLERLKGLPDGETKAKETKRMIDIVRNFSGYREYPK 183 Sbjct: 611 670 Query: 184 YAWMNHYFIYKQALLKEAARLVQAGVIHEKEDIYHLSFEELHEAVRTHKLDYQIISKRKE 243 Sbjct: 671 730 Query: 244 EYKLYDKLTPPRVITSDGEIIAGEYKRENLPAGAIVGLAVSSEI 287 Sbjct: 731 774 >BS_ppsA_1 [Q] COG1020 Non-ribosomal peptide synthetase modules and related proteins Length = 792 Score = 488 bits (1256), Expect = e-138, Method: Compositional matrix adjust. Identities = 20/284 (7%), Positives = 54/284 (19%) Query: 4 MNASRWINENMNEWLGEKNAADTLSQSVRDNVTSEMGLELMDVADVIRPYPEVIDYLQYV 63 N R + G A + V LE D ++ + Sbjct: 491 GNPDRAFDFACLPNEGVGLARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDS 550 Query: 64 KADNFLDELHKFDGGQETRDAIYAFLQKYGMHCTGEIDITRTRWSEKPSMLVPMILGNIR 123 + ++ L + + + + E +P PM+ Sbjct: 551 PREFYVGRLTEGIATLGAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGA 610 Query: 124 NFEPHAGGLKFEHGRQEAMAKEQELLERLKGLPDGETKAKETKRMIDIVRNFSGYREYPK 183 EA+ + + + + + + + Sbjct: 611 GRYVSDSFRDCFALECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGE 670 Query: 184 YAWMNHYFIYKQALLKEAARLVQAGVIHEKEDIYHLSFEELHEAVRTHKLDYQIISKRKE 243 + A + ++ + + Sbjct: 671 NGLKIIMMCEIPSNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAV 730 Query: 244 EYKLYDKLTPPRVITSDGEIIAGEYKRENLPAGAIVGLAVSSEI 287 + L + + I A ++ + S Sbjct: 731 KALLSMAIRAAKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLS 774 >BS_ppsA_2 [Q] COG1020 Non-ribosomal peptide synthetase modules and related proteins Length = 792 Score = 488 bits (1256), Expect = e-138, Method: Compositional matrix adjust. Identities = 20/284 (7%), Positives = 54/284 (19%) Query: 4 MNASRWINENMNEWLGEKNAADTLSQSVRDNVTSEMGLELMDVADVIRPYPEVIDYLQYV 63 N R + G A + V LE D ++ + Sbjct: 491 GNPDRAFDFACLPNEGVGLARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDS 550 Query: 64 KADNFLDELHKFDGGQETRDAIYAFLQKYGMHCTGEIDITRTRWSEKPSMLVPMILGNIR 123 + ++ L + + + + E +P PM+ Sbjct: 551 PREFYVGRLTEGIATLGAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGA 610 Query: 124 NFEPHAGGLKFEHGRQEAMAKEQELLERLKGLPDGETKAKETKRMIDIVRNFSGYREYPK 183 EA+ + + + + + + + Sbjct: 611 GRYVSDSFRDCFALECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGE 670 Query: 184 YAWMNHYFIYKQALLKEAARLVQAGVIHEKEDIYHLSFEELHEAVRTHKLDYQIISKRKE 243 + A + ++ + + Sbjct: 671 NGLKIIMMCEIPSNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAV 730 Query: 244 EYKLYDKLTPPRVITSDGEIIAGEYKRENLPAGAIVGLAVSSEI 287 + L + + I A ++ + S Sbjct: 731 KALLSMAIRAAKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLS 774 >BS_pps [G] COG0574 Phosphoenolpyruvate synthase/pyruvate phosphate dikinase Length = 866 Score = 488 bits (1256), Expect = e-138, Method: Compositional matrix adjust. Identities = 229/284 (80%), Positives = 253/284 (89%) Query: 4 MNASRWINENMNEWLGEKNAADTLSQSVRDNVTSEMGLELMDVADVIRPYPEVIDYLQYV 63 MNAS WINE MNEWLGEKNAADTLSQSV N+TSEMGL L+DVADVIRPYPEVIDYLQ+V Sbjct: 491 MNASLWINEKMNEWLGEKNAADTLSQSVPHNITSEMGLALLDVADVIRPYPEVIDYLQHV 550 Query: 64 KADNFLDELHKFDGGQETRDAIYAFLQKYGMHCTGEIDITRTRWSEKPSMLVPMILGNIR 123 K DNFLDEL KFDGG +TRDAIY +L KYGM CTGEIDITRTRWSEKP+ LVPMIL NI+ Sbjct: 551 KDDNFLDELAKFDGGSKTRDAIYDYLSKYGMRCTGEIDITRTRWSEKPTTLVPMILNNIK 610 Query: 124 NFEPHAGGLKFEHGRQEAMAKEQELLERLKGLPDGETKAKETKRMIDIVRNFSGYREYPK 183 NFEP+ G KFE GRQEA+ KEQELL+RLK LPDGE KAKETKR IDI+RNFSG+REYPK Sbjct: 611 NFEPNVGHRKFEQGRQEALKKEQELLDRLKQLPDGEQKAKETKRAIDIIRNFSGFREYPK 670 Query: 184 YAWMNHYFIYKQALLKEAARLVQAGVIHEKEDIYHLSFEELHEAVRTHKLDYQIISKRKE 243 Y +N YF+YKQALLKEA +L++AGVIHEKEDIY+L+FEELHE VRTHKLDYQIIS RK+ Sbjct: 671 YGMVNRYFVYKQALLKEAEQLIEAGVIHEKEDIYYLTFEELHEVVRTHKLDYQIISTRKD 730 Query: 244 EYKLYDKLTPPRVITSDGEIIAGEYKRENLPAGAIVGLAVSSEI 287 EY LY+KL+PPRVITSDGEI+ GEYKRENLPAGAIVGL VSS + Sbjct: 731 EYTLYEKLSPPRVITSDGEIVTGEYKRENLPAGAIVGLPVSSGV 774 >CAC0534 [G] COG0574 Phosphoenolpyruvate synthase/pyruvate phosphate dikinase Length = 868 Score = 436 bits (1122), Expect = e-122, Method: Compositional matrix adjust. Identities = 201/287 (70%), Positives = 242/287 (84%) Query: 1 MAVMNASRWINENMNEWLGEKNAADTLSQSVRDNVTSEMGLELMDVADVIRPYPEVIDYL 60 M +NAS WINE M +WLGEKNAAD LSQSV +N+TS+MGLEL+++ADVIRPYPE+IDYL Sbjct: 490 MTAINASHWINEKMRDWLGEKNAADILSQSVPNNITSKMGLELLEIADVIRPYPEIIDYL 549 Query: 61 QYVKADNFLDELHKFDGGQETRDAIYAFLQKYGMHCTGEIDITRTRWSEKPSMLVPMILG 120 Q+VK DNFL+E+ KF+GG E ++AI ++L KYGM C+GEIDITRTR SEKP LVPMIL Sbjct: 550 QHVKDDNFLEEIVKFNGGHEVKNAILSYLSKYGMRCSGEIDITRTRLSEKPITLVPMILN 609 Query: 121 NIRNFEPHAGGLKFEHGRQEAMAKEQELLERLKGLPDGETKAKETKRMIDIVRNFSGYRE 180 NI+NFE A +KF+ G+Q+A+ KEQELLERLK LPDGE KAKETKRMI ++RNF GYRE Sbjct: 610 NIKNFETGAANIKFQQGKQKALEKEQELLERLKQLPDGEQKAKETKRMIKLIRNFIGYRE 669 Query: 181 YPKYAWMNHYFIYKQALLKEAARLVQAGVIHEKEDIYHLSFEELHEAVRTHKLDYQIISK 240 YPKY +N YFIYKQAL+KEA +L G IHEKEDIY+L+FEEL E +RT K+DY II+K Sbjct: 670 YPKYGMVNRYFIYKQALMKEAEKLANIGAIHEKEDIYYLTFEELREVIRTKKVDYSIINK 729 Query: 241 RKEEYKLYDKLTPPRVITSDGEIIAGEYKRENLPAGAIVGLAVSSEI 287 RK E+KLY+KL PPRVITSDGEI+ GEYKREN+P GAI+GL+VSS + Sbjct: 730 RKNEHKLYEKLIPPRVITSDGEIVEGEYKRENVPEGAIIGLSVSSGV 776 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.135 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,073,525 Number of extensions: 927410 Number of successful extensions: 1749 Number of sequences better than 1.0e-05: 8 Number of HSP's gapped: 1748 Number of HSP's successfully gapped: 8 Length of query: 288 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 183 Effective length of database: 26,268,361 Effective search space: 4807110063 Effective search space used: 4807110063 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 115 (48.9 bits)