BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0562.0004.p (590 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ZuidAm [G] COG3250 Beta-galactosidase/beta-glucuronidase 85 4e-16 uidA [G] COG3250 Beta-galactosidase/beta-glucuronidase 84 9e-16 ZuidAm [G] COG3250 Beta-galactosidase/beta-glucuronidase 84 9e-16 ZebgA [G] COG3250 Beta-galactosidase/beta-glucuronidase 82 2e-15 ebgA [G] COG3250 Beta-galactosidase/beta-glucuronidase 82 2e-15 >ZuidAm [G] COG3250 Beta-galactosidase/beta-glucuronidase Length = 603 Score = 84.7 bits (208), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 60/608 (9%), Positives = 142/608 (23%), Gaps = 94/608 (15%) Query: 26 LNGEWDFSFDDDEAGVAERWMEH-FPAAQKINVPFTYETRASGIGEEKFHAQIWYRRKWI 84 L+G W FS D + G+ +RW E ++ I VP ++ + + + +WY+R+ Sbjct: 15 LDGLWAFSLDRENCGIDQRWWESALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVF 74 Query: 85 LSPEAEGKQTILHFEGVDYKATCYVNGSFAGEHEGAYARFSLDISGLLKQDAENEIVLKV 144 + G++ +L F+ V + +VN EH+G Y F D++ + I + V Sbjct: 75 IPKGWAGQRIVLRFDAVTHYGKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCV 134 Query: 145 EDSDS--CLQP-------RGKQRWAGRNHDSFYVQTTGIWKSVWLEHVHETRLDTVKMTP 195 + + + P GK++ HD F GI +SV L T +D + + Sbjct: 135 NNELNWQTIPPGMVITDENGKKK-QSYFHDFF--NYAGIHRSVMLYTTPNTWVDDITVV- 190 Query: 196 DIDRQMIRFDFRMHGTKNKKDLRLETEITFKGETVRKLSLSVDRAWLTAETGMMSGINGP 255 H ++ ++ ++ G+ +L + + T + + Sbjct: 191 ------------THVAQDCNHASVDWQVVANGDVSVELRDADQQVVATGQGT-SGTLQVV 237 Query: 256 WLQNLWSPDHPNLFDVEFVLYEGDRELDRVGSYFGMRKISIRDGQILLNNIPLYQRLILD 315 + + ++ + + Sbjct: 238 NPHLWQPGEGYLYELC-VTAKSQTECDIYPLRVGIRSVAVKGEQFLINHKPFYF---TGF 293 Query: 316 QGYWTESHLTPPSXXXXXXXXXXXXG--MGYNGLRKHMKIEDARFLYWCDVKGMLVWSEM 373 + + H + + + +++ Sbjct: 294 GRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWADEHGIVVIDETA 353 Query: 374 AA---------AFE--------YNDEAXXXRFTKEWI----EVVQQQYNHPCIITWVPFN 412 A + + + + + ++ + Sbjct: 354 AVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDKNHPSVVMWSIANE 413 Query: 413 ESWGIQNISHDDRQQKFTEGIYYL---TKSIDPHRP-----VITNDGWEHTIS---DILT 461 Q K + + + + Sbjct: 414 PD--------TRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLFDVLCL 465 Query: 462 LHDYVERGEDLYKTYKDKDSITGSSGTYNEWKFAFAHGYSYKGQPIIISEFGGIAFRSEH 521 Y + D T E + II Sbjct: 466 NRYY-------GWYVQSGDLETAEKVLEKELLAWQEKLHQ---PIIITEYGVDTLAGLHS 515 Query: 522 G-----WGYGNQ---VDNEEAFLERFGSITGAIKAIPFISGYCYTQ-ITDVQHEVNGLLT 572 F + + F + ++ Sbjct: 516 MYTDMWSEEYQCAWLDMYHRVFDRVSAVVGE--QVWNFADFATSQGILRVGGNKKGIFTR 573 Query: 573 EGRKPKVA 580 + + A Sbjct: 574 DRKPKSAA 581 >uidA [G] COG3250 Beta-galactosidase/beta-glucuronidase Length = 603 Score = 83.6 bits (205), Expect = 9e-16, Method: Compositional matrix adjust. Identities = 133/610 (21%), Positives = 229/610 (37%), Gaps = 99/610 (16%) Query: 26 LNGEWDFSFDDDEAGVAERWMEH-FPAAQKINVPFTYETRASGIGEEKFHAQIWYRRKWI 84 L+G W FS D + G+ +RW E ++ I VP ++ + + + +WY+R+ Sbjct: 15 LDGLWAFSLDRENCGIDQRWWESALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVF 74 Query: 85 LSPEAEGKQTILHFEGVDYKATCYVNGSFAGEHEGAYARFSLDISGLLKQDAENEIVLKV 144 + G++ +L F+ V + +VN EH+G Y F D++ + I + V Sbjct: 75 IPKGWAGQRIVLRFDAVTHYGKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCV 134 Query: 145 EDSDS--CLQP-------RGKQRWAGRNHDSFYVQTTGIWKSVWLEHVHETRLDTVKMTP 195 + + + P GK++ HD F GI +SV L T +D + + Sbjct: 135 NNELNWQTIPPGMVITDENGKKK-QSYFHDFF--NYAGIHRSVMLYTTPNTWVDDITVV- 190 Query: 196 DIDRQMIRFDFRMHGTKNKKDLRLETEITFKGETVRKLSLSVDRAWLTAE--TGMMSGIN 253 H ++ ++ ++ G+ +L + + T + +G + +N Sbjct: 191 ------------THVAQDCNHASVDWQVVANGDVSVELRDADQQVVATGQGTSGTLQVVN 238 Query: 254 GPWLQNLWSPDHPNLFDVEFVLYEGDRELDRVGSYFGMRKISIRDGQILLNNIPLYQRLI 313 +LW P L+++ V + E D G+R ++++ Q L+N+ P Y Sbjct: 239 ----PHLWQPGEGYLYEL-CVTAKSQTECDIYPLRVGIRSVAVKGEQFLINHKPFY---F 290 Query: 314 LDQGYWTESHLTPPSXXXXXXXXXXXXG--MGYNGLRKHMKIEDARFLYWCDVKGMLVWS 371 G ++ L +G N R L W D G++V Sbjct: 291 TGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWADEHGIVVID 350 Query: 372 EMAA---------AFE--------YNDEAXXXRFTKEWI----EVVQQQYNHPCIITWVP 410 E AA FE Y++EA + + E++ + NHP ++ W Sbjct: 351 ETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDKNHPSVVMWSI 410 Query: 411 FNESWGIQNISHDDRQQKFTEGIYYL---TKSIDPHRP-----VITNDGWEHTIS---DI 459 NE D R Q E L T+ +DP RP V+ D TIS D+ Sbjct: 411 ANEP--------DTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLFDV 462 Query: 460 LTLHDYVERGEDLYKTYKDKDSITGSSGTYNEWKFAFAHGYSYKGQPIIISEFGGIAFRS 519 L L+ Y Y Y + + + A+ QPIII+E+G Sbjct: 463 LCLNRY-------YGWYVQSGDLETAEKVLEKELLAWQEKLH---QPIIITEYGVDTLAG 512 Query: 520 EHG-----WGYGNQ---VDNEEAFLERFGSITGAIKAIPFISGYCYTQ-ITDVQHEVNGL 570 H W Q +D +R ++ G + + + + +Q I V G+ Sbjct: 513 LHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVG--EQVWNFADFATSQGILRVGGNKKGI 570 Query: 571 LTEGRKPKVA 580 T RKPK A Sbjct: 571 FTRDRKPKSA 580 Score = 83.6 bits (205), Expect = 9e-16, Method: Compositional matrix adjust. Identities = 133/610 (21%), Positives = 229/610 (37%), Gaps = 99/610 (16%) Query: 26 LNGEWDFSFDDDEAGVAERWMEH-FPAAQKINVPFTYETRASGIGEEKFHAQIWYRRKWI 84 L+G W FS D + G+ +RW E ++ I VP ++ + + + +WY+R+ Sbjct: 15 LDGLWAFSLDRENCGIDQRWWESALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVF 74 Query: 85 LSPEAEGKQTILHFEGVDYKATCYVNGSFAGEHEGAYARFSLDISGLLKQDAENEIVLKV 144 + G++ +L F+ V + +VN EH+G Y F D++ + I + V Sbjct: 75 IPKGWAGQRIVLRFDAVTHYGKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCV 134 Query: 145 EDSDS--CLQP-------RGKQRWAGRNHDSFYVQTTGIWKSVWLEHVHETRLDTVKMTP 195 + + + P GK++ HD F GI +SV L T +D + + Sbjct: 135 NNELNWQTIPPGMVITDENGKKK-QSYFHDFF--NYAGIHRSVMLYTTPNTWVDDITVV- 190 Query: 196 DIDRQMIRFDFRMHGTKNKKDLRLETEITFKGETVRKLSLSVDRAWLTAE--TGMMSGIN 253 H ++ ++ ++ G+ +L + + T + +G + +N Sbjct: 191 ------------THVAQDCNHASVDWQVVANGDVSVELRDADQQVVATGQGTSGTLQVVN 238 Query: 254 GPWLQNLWSPDHPNLFDVEFVLYEGDRELDRVGSYFGMRKISIRDGQILLNNIPLYQRLI 313 +LW P L+++ V + E D G+R ++++ Q L+N+ P Y Sbjct: 239 ----PHLWQPGEGYLYEL-CVTAKSQTECDIYPLRVGIRSVAVKGEQFLINHKPFY---F 290 Query: 314 LDQGYWTESHLTPPSXXXXXXXXXXXXG--MGYNGLRKHMKIEDARFLYWCDVKGMLVWS 371 G ++ L +G N R L W D G++V Sbjct: 291 TGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWADEHGIVVID 350 Query: 372 EMAA---------AFE--------YNDEAXXXRFTKEWI----EVVQQQYNHPCIITWVP 410 E AA FE Y++EA + + E++ + NHP ++ W Sbjct: 351 ETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDKNHPSVVMWSI 410 Query: 411 FNESWGIQNISHDDRQQKFTEGIYYL---TKSIDPHRP-----VITNDGWEHTIS---DI 459 NE D R Q E L T+ +DP RP V+ D TIS D+ Sbjct: 411 ANEP--------DTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLFDV 462 Query: 460 LTLHDYVERGEDLYKTYKDKDSITGSSGTYNEWKFAFAHGYSYKGQPIIISEFGGIAFRS 519 L L+ Y Y Y + + + A+ QPIII+E+G Sbjct: 463 LCLNRY-------YGWYVQSGDLETAEKVLEKELLAWQEKLH---QPIIITEYGVDTLAG 512 Query: 520 EHG-----WGYGNQ---VDNEEAFLERFGSITGAIKAIPFISGYCYTQ-ITDVQHEVNGL 570 H W Q +D +R ++ G + + + + +Q I V G+ Sbjct: 513 LHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVG--EQVWNFADFATSQGILRVGGNKKGI 570 Query: 571 LTEGRKPKVA 580 T RKPK A Sbjct: 571 FTRDRKPKSA 580 >ZebgA [G] COG3250 Beta-galactosidase/beta-glucuronidase Length = 1042 Score = 82.0 bits (201), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 116/485 (23%), Positives = 187/485 (38%), Gaps = 80/485 (16%) Query: 21 SEWLDLNGEWDFSFDDDEAGVAERWMEHFPAA-QKINVPFTYETRASG---IGEEKFHAQ 76 S +L L+G+W+F F D V E + A I VP ++ G +E F Sbjct: 51 SLFLPLSGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFP 110 Query: 77 I------------WYRRKWILSPEAEGKQTILHFEGVDYKATCYVNGSFAGEHEGAYARF 124 I Y+R + LS +GKQT++ F+GV+ YVNG + G +G+ Sbjct: 111 IDVPFVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTA 170 Query: 125 SLDISGLLKQDAENEIVLKVEDSDSCLQPRGKQRWAGRNHDSFYVQ------TTGIWKSV 178 DIS ++K +N + ++V +WA DS YV+ + GI++ V Sbjct: 171 EFDISAMVKT-GDNLLCVRV------------MQWA----DSTYVEDQDMWWSAGIFRDV 213 Query: 179 WLEHVHETRLDTVKMTPDIDRQMIRFDFRMHGTKNKKDLRLETEIT------FKGETVRK 232 +L H T ++ + D D D + ++L +T F GE V Sbjct: 214 YLVGKHLTHINDFTVRTDFDEAYC--DATLSCEVVLENLAASPVVTTLEYTLFDGERVVH 271 Query: 233 LSLSVDRAWLTAETGMMSGINGPWLQNLWSPDHPNLFDVEFVLYEGD-RELDRVGSYFGM 291 S ++D + T Q WS + P L+ + L + + L+ V G Sbjct: 272 -SSAIDHLAIEKLTSASFAFTVEQPQQ-WSAESPYLYHLVMTLKDANGNVLEVVPQRVGF 329 Query: 292 RKISIRDGQILLNNIPLYQRLILDQGYWTESHLTPPSXXXXXXXXXXXXGM----GYNGL 347 R I +RDG +NN R ++ G + + N + Sbjct: 330 RDIKVRDGLFWINN-----RYVMLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSV 384 Query: 348 RKHMKIEDARFLYWCDVKGMLVWSEM-AAAFEYNDEAXXXRFT--KEWIEV--------V 396 R D RF CD+ G+ V +E + + + R T +W +V + Sbjct: 385 RTAHYPNDPRFYELCDIYGLFVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHI 444 Query: 397 QQQYNHPCIITWVPFNESWGIQNISHDDRQQKFTEGIYYLTKSIDPHRPVITNDGWEHTI 456 Q NHP II W NES NI +Y+ K++D R V + + + Sbjct: 445 HAQKNHPSIIIWSLGNESGYGCNI----------RAMYHAAKALDDTRLVHYEEDRDAEV 494 Query: 457 SDILT 461 DI++ Sbjct: 495 VDIIS 499 >ebgA [G] COG3250 Beta-galactosidase/beta-glucuronidase Length = 1042 Score = 82.0 bits (201), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 116/485 (23%), Positives = 187/485 (38%), Gaps = 80/485 (16%) Query: 21 SEWLDLNGEWDFSFDDDEAGVAERWMEHFPAA-QKINVPFTYETRASG---IGEEKFHAQ 76 S +L L+G+W+F F D V E + A I VP ++ G +E F Sbjct: 51 SLFLPLSGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFP 110 Query: 77 I------------WYRRKWILSPEAEGKQTILHFEGVDYKATCYVNGSFAGEHEGAYARF 124 I Y+R + LS +GKQT++ F+GV+ YVNG + G +G+ Sbjct: 111 IDVPFVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTA 170 Query: 125 SLDISGLLKQDAENEIVLKVEDSDSCLQPRGKQRWAGRNHDSFYVQ------TTGIWKSV 178 DIS ++K +N + ++V +WA DS YV+ + GI++ V Sbjct: 171 EFDISAMVKT-GDNLLCVRV------------MQWA----DSTYVEDQDMWWSAGIFRDV 213 Query: 179 WLEHVHETRLDTVKMTPDIDRQMIRFDFRMHGTKNKKDLRLETEIT------FKGETVRK 232 +L H T ++ + D D D + ++L +T F GE V Sbjct: 214 YLVGKHLTHINDFTVRTDFDEAYC--DATLSCEVVLENLAASPVVTTLEYTLFDGERVVH 271 Query: 233 LSLSVDRAWLTAETGMMSGINGPWLQNLWSPDHPNLFDVEFVLYEGD-RELDRVGSYFGM 291 S ++D + T Q WS + P L+ + L + + L+ V G Sbjct: 272 -SSAIDHLAIEKLTSATFAFTVEQPQQ-WSAESPYLYHLVMTLKDANGNVLEVVPQRVGF 329 Query: 292 RKISIRDGQILLNNIPLYQRLILDQGYWTESHLTPPSXXXXXXXXXXXXGM----GYNGL 347 R I +RDG +NN R ++ G + + N + Sbjct: 330 RDIKVRDGLFWINN-----RYVMLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSV 384 Query: 348 RKHMKIEDARFLYWCDVKGMLVWSEM-AAAFEYNDEAXXXRFT--KEWIEV--------V 396 R D RF CD+ G+ V +E + + + R T +W +V + Sbjct: 385 RTAHYPNDPRFYELCDIYGLFVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHI 444 Query: 397 QQQYNHPCIITWVPFNESWGIQNISHDDRQQKFTEGIYYLTKSIDPHRPVITNDGWEHTI 456 Q NHP II W NES NI +Y+ K++D R V + + + Sbjct: 445 HAQKNHPSIIIWSLGNESGYGCNI----------RAMYHAAKALDDTRLVHYEEDRDAEV 494 Query: 457 SDILT 461 DI++ Sbjct: 495 VDIIS 499 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.137 0.436 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 46,764,423 Number of extensions: 2065573 Number of successful extensions: 3078 Number of sequences better than 1.0e-05: 9 Number of HSP's gapped: 3066 Number of HSP's successfully gapped: 9 Length of query: 590 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 478 Effective length of database: 25,461,023 Effective search space: 12170368994 Effective search space used: 12170368994 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 119 (50.4 bits)