BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0562.0003.p (220 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value NMA2151 [EH] COG0115 Branched-chain amino acid aminotransferase/... 85 9e-17 NMA1319 [R] COG4228 Mu-like prophage DNA circulation protein 84 2e-16 all0772 [QR] COG0500 SAM-dependent methyltransferases 80 2e-15 AF0140 [QR] COG0500 SAM-dependent methyltransferases 74 2e-13 ML0551 [QR] COG0500 SAM-dependent methyltransferases 70 3e-12 >NMA2151 [EH] COG0115 Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase Length = 261 Score = 84.7 bits (208), Expect = 9e-17, Method: Compositional matrix adjust. Identities = 58/145 (40%), Positives = 81/145 (55%), Gaps = 16/145 (11%) Query: 46 KKALS---GLDLKPGQQVLLVGAGTGADLPLISGRGLSITAIDLSPAMLAEARSK-IKEG 101 KK LS G DLK +L VG+GTG ++ G +TA+D S M+ AR K +++G Sbjct: 45 KKLLSSSIGPDLK---NILDVGSGTGIIAMYLAELGYGVTAVDFSEGMMDIARKKALEKG 101 Query: 102 EKITFLEMDAQDLRFGDETFDVVIANLILSVVPDAERCMQEIVRVTSHGGKIVIFD-KFA 160 KI F+E D ++L F DETFD V A +L +P E+ ++E VRV GG+IVI D K+ Sbjct: 102 AKIRFMEGDIENLSFEDETFDCVTARYVLWTLPHPEKALKEWVRVVKPGGRIVIIDGKWV 161 Query: 161 SNGRVSPVMKVMRPLISVLGTDIGR 185 + G + P IS + I R Sbjct: 162 TRG--------LLPRISAMNYHIYR 178 >NMA1319 [R] COG4228 Mu-like prophage DNA circulation protein Length = 199 Score = 84.0 bits (206), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 58/175 (33%), Positives = 96/175 (54%), Gaps = 13/175 (7%) Query: 45 RKKALSGLDLKPGQQVLLVGAGTGADLPLISGRGLSITAIDLSPAMLAEARSKIKEGEKI 104 R++ LS L +VL VG GTG +L S G S+T ID+S ML +AR K + + + Sbjct: 30 REETLSDLQ----GRVLEVGVGTGRNLKYYSS-GSSVTGIDVSEGMLEKARKKAEGMKNV 84 Query: 105 TFLEMDAQDLRFGDETFDVVIANLILSVVPDAERCMQEIVRVTSHGGKIVIFDKF-ASNG 163 L MDA+ L F D+TFD VI +L +PD + ++E+ RV G+++ + +SN Sbjct: 85 NLLLMDAEHLEFSDKTFDYVITTFVLCSIPDPVKALKEMRRVLKPSGELIAIEHMRSSNN 144 Query: 164 RVSPVMKVMRPLI-SVLGTDIGRDF--NLLFLPYNEQLSMKEESPLLMRGMYRKI 215 +S ++ P++ S++G ++ RD N+ +KEE L + +++KI Sbjct: 145 LISIFETLINPIMFSIIGDEVTRDTVGNI----RKAGFIIKEEKNLAFKDVFKKI 195 >all0772 [QR] COG0500 SAM-dependent methyltransferases Length = 205 Score = 80.5 bits (197), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 49/162 (30%), Positives = 81/162 (50%), Gaps = 4/162 (2%) Query: 12 TGDYMNNSWNRIIYKLWAPLYDRVFNSGAFAGARKKALSGLDLKPGQQVLLVGAGTGADL 71 T D++ N + I+ WA YD +F S + K+ L +DL +L +G GTG L Sbjct: 3 TNDFLKN--KKFIFDRWANSYDWLFPSIFYQAIHKRLLEFVDLSQPANILDLGCGTGRLL 60 Query: 72 PLISGR--GLSITAIDLSPAMLAEARSKIKEGEKITFLEMDAQDLRFGDETFDVVIANLI 129 ++ + L T +DLS ML +AR + ++ FLE A+ L FGD FD V + Sbjct: 61 ERLANKFPELRGTGLDLSSNMLRQARLSNRHHPRLIFLEGKAESLPFGDGQFDAVFNTIS 120 Query: 130 LSVVPDAERCMQEIVRVTSHGGKIVIFDKFASNGRVSPVMKV 171 + E+ +QE+ RV S GG+ + D + ++ ++ + Sbjct: 121 FLHYREPEKVLQEVSRVLSPGGRFYLVDFTTTREKLPEILPI 162 >AF0140 [QR] COG0500 SAM-dependent methyltransferases Length = 261 Score = 73.9 bits (180), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 42/128 (32%), Positives = 66/128 (51%), Gaps = 5/128 (3%) Query: 60 VLLVGAGTGADLPLISGRGLSITAIDLSPAMLAEARSKIKE-GEKITFLEMDAQDLRFGD 118 VL +GAGTG ++P ++ A+D+S ML A + KE G+K+ FL DA++L F Sbjct: 64 VLEIGAGTGKNIPHYPS---NVVALDISEKMLERAVRRAKESGKKVDFLLADAENLPFRS 120 Query: 119 ETFDVVIANLILSVVPDAERCMQEIVRVTSHGGKIVIFDKFASNGR-VSPVMKVMRPLIS 177 +FDVV + V D R ++E RV GG+ + R + P+ ++ P+ Sbjct: 121 NSFDVVFTTFVFCSVDDPVRGLREAFRVLKKGGRAYFLEHMLPKSRFLHPLFNLLNPIFR 180 Query: 178 VLGTDIGR 185 G +I R Sbjct: 181 AFGPEINR 188 >ML0551 [QR] COG0500 SAM-dependent methyltransferases Length = 274 Score = 69.7 bits (169), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 45/134 (33%), Positives = 73/134 (54%), Gaps = 5/134 (3%) Query: 49 LSGLDLKPGQQVLLVGAGTG-ADLPLISGRGLSITAIDLSPAMLAEARSKIKE-GEKITF 106 ++ ++PG QVL V AG+G LP + G +I + DL+P +L ++++ E G + + Sbjct: 42 VTATGIRPGMQVLDVAAGSGNISLP-AAKTGATIVSTDLTPELLQRSQARATELGLTLEY 100 Query: 107 LEMDAQDLRFGDETFDVVIANLILSVVPDAERCMQEIVRVTSHGGKI--VIFDKFASNGR 164 E DAQ L FGD FDVV++ + + PD R E+VRV G I + + + GR Sbjct: 101 READAQALPFGDGEFDVVMSAIGVMFAPDHRRAASELVRVCRPNGTIGVISWTQEGFFGR 160 Query: 165 VSPVMKVMRPLISV 178 + ++ RP +S Sbjct: 161 MLATIRPYRPSLSA 174 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.139 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 16,136,257 Number of extensions: 644599 Number of successful extensions: 2958 Number of sequences better than 1.0e-05: 32 Number of HSP's gapped: 4280 Number of HSP's successfully gapped: 32 Length of query: 220 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 118 Effective length of database: 26,614,363 Effective search space: 3140494834 Effective search space used: 3140494834 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 114 (48.5 bits)