BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0562.0002.p (386 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ppsE_2 [Q] COG3319 Thioesterase domains of type I polyketide ... 649 0.0 BS_ppsA_1 [Q] COG1020 Non-ribosomal peptide synthetase modules a... 649 0.0 BS_ppsA_2 [Q] COG1020 Non-ribosomal peptide synthetase modules a... 649 0.0 BS_pps [G] COG0574 Phosphoenolpyruvate synthase/pyruvate phospha... 649 0.0 CAC0534 [G] COG0574 Phosphoenolpyruvate synthase/pyruvate phosph... 522 e-148 >BS_ppsE_2 [Q] COG3319 Thioesterase domains of type I polyketide synthases or non-ribosomal peptide synthetases Length = 211 Score = 649 bits (1673), Expect = 0.0, Method: Compositional matrix adjust. Identities = 0/370 (0%), Positives = 0/370 (0%) Query: 1 MSSLVLGFQEMDKTQLWLVGGKGLHLGELSKIQGIQVPEGFCVTTAGYQKAIEQNETYHA 60 M+ + L+ ++ + + + + + Sbjct: 1 MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANT 60 Query: 61 LLDQLTRLKVEDRAQIGEISRQIRQIITEAEIPSDVVKAVAQYLSLFGEKYAYAVRSSAT 120 L + I I + + +++ + Sbjct: 61 PAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNGEVPEDRAALEALPGVGRK 120 Query: 121 AEDLPHASFAGQQDTYLNIIGKDAILQHISKCWASLFTERAVIYRMQNGFDHRQVYLSVV 180 ++ + G ++ + ++ + ++ + ++ Sbjct: 121 TANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVEEKLLKVVPAEFKVDCHHWLI 180 Query: 181 IQKMVFPQASGIVFTADPMTSNRKLLSIDASFGLGEALVSGLVSADCYKVREEEIVEKRI 240 Sbjct: 181 240 Query: 241 ATKKLAIYGRQEGGTETRQLDPGQQTAQTLTDGEILELARIGRQIEAYFGQPQDIEWCLA 300 Sbjct: 241 300 Query: 301 DGTFYIVQSRPITTLYPIPEADDQENHVYVSVGHQQMMTDPIKPLGLSFYLLMTPALMRK 360 Sbjct: 301 360 Query: 361 AGGRLFLDVA 370 Sbjct: 361 370 >BS_ppsA_1 [Q] COG1020 Non-ribosomal peptide synthetase modules and related proteins Length = 792 Score = 649 bits (1673), Expect = 0.0, Method: Compositional matrix adjust. Identities = 26/370 (7%), Positives = 78/370 (21%) Query: 1 MSSLVLGFQEMDKTQLWLVGGKGLHLGELSKIQGIQVPEGFCVTTAGYQKAIEQNETYHA 60 MS+ + QL + + S + I G V+ Sbjct: 1 MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVSVPNGFATTADAFNQFL 60 Query: 61 LLDQLTRLKVEDRAQIGEISRQIRQIITEAEIPSDVVKAVAQYLSLFGEKYAYAVRSSAT 120 + + E + + L + + + Sbjct: 61 DQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQPELENAIREAYAQLSADDE 120 Query: 121 AEDLPHASFAGQQDTYLNIIGKDAILQHISKCWASLFTERAVIYRMQNGFDHRQVYLSVV 180 S A +D + + ++ ++ + Sbjct: 121 NASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAVKHVFASLFNDRAISYRVHQG 180 Query: 181 IQKMVFPQASGIVFTADPMTSNRKLLSIDASFGLGEALVSGLVSADCYKVREEEIVEKRI 240 ++G+ ++ ++ + + +V + ++ + V Sbjct: 181 YDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQVVFITSAWGLGEMVVQGAVNPDE 240 Query: 241 ATKKLAIYGRQEGGTETRQLDPGQQTAQTLTDGEILELARIGRQIEAYFGQPQDIEWCLA 300 R + + E + +I + + Sbjct: 241 FYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHGKQVKIEDVPQEQRDIFSLTNEEVQ 300 Query: 301 DGTFYIVQSRPITTLYPIPEADDQENHVYVSVGHQQMMTDPIKPLGLSFYLLMTPALMRK 360 + VQ E + + + + T + + Y L + + Sbjct: 301 ELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQARPETVRSRGQVMERYTLHSQGKIIA 360 Query: 361 AGGRLFLDVA 370 G + + Sbjct: 361 EGRAIGHRIG 370 >BS_ppsA_2 [Q] COG1020 Non-ribosomal peptide synthetase modules and related proteins Length = 792 Score = 649 bits (1673), Expect = 0.0, Method: Compositional matrix adjust. Identities = 26/370 (7%), Positives = 78/370 (21%) Query: 1 MSSLVLGFQEMDKTQLWLVGGKGLHLGELSKIQGIQVPEGFCVTTAGYQKAIEQNETYHA 60 MS+ + QL + + S + I G V+ Sbjct: 1 MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVSVPNGFATTADAFNQFL 60 Query: 61 LLDQLTRLKVEDRAQIGEISRQIRQIITEAEIPSDVVKAVAQYLSLFGEKYAYAVRSSAT 120 + + E + + L + + + Sbjct: 61 DQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQPELENAIREAYAQLSADDE 120 Query: 121 AEDLPHASFAGQQDTYLNIIGKDAILQHISKCWASLFTERAVIYRMQNGFDHRQVYLSVV 180 S A +D + + ++ ++ + Sbjct: 121 NASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAVKHVFASLFNDRAISYRVHQG 180 Query: 181 IQKMVFPQASGIVFTADPMTSNRKLLSIDASFGLGEALVSGLVSADCYKVREEEIVEKRI 240 ++G+ ++ ++ + + +V + ++ + V Sbjct: 181 YDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQVVFITSAWGLGEMVVQGAVNPDE 240 Query: 241 ATKKLAIYGRQEGGTETRQLDPGQQTAQTLTDGEILELARIGRQIEAYFGQPQDIEWCLA 300 R + + E + +I + + Sbjct: 241 FYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHGKQVKIEDVPQEQRDIFSLTNEEVQ 300 Query: 301 DGTFYIVQSRPITTLYPIPEADDQENHVYVSVGHQQMMTDPIKPLGLSFYLLMTPALMRK 360 + VQ E + + + + T + + Y L + + Sbjct: 301 ELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQARPETVRSRGQVMERYTLHSQGKIIA 360 Query: 361 AGGRLFLDVA 370 G + + Sbjct: 361 EGRAIGHRIG 370 >BS_pps [G] COG0574 Phosphoenolpyruvate synthase/pyruvate phosphate dikinase Length = 866 Score = 649 bits (1673), Expect = 0.0, Method: Compositional matrix adjust. Identities = 311/370 (84%), Positives = 341/370 (92%) Query: 1 MSSLVLGFQEMDKTQLWLVGGKGLHLGELSKIQGIQVPEGFCVTTAGYQKAIEQNETYHA 60 MSSLVLG E++KTQL LVGGKGLHLGELSKIQGIQVPEGFCVTT GYQKAIEQNET Sbjct: 1 MSSLVLGLHEIEKTQLSLVGGKGLHLGELSKIQGIQVPEGFCVTTVGYQKAIEQNETLQV 60 Query: 61 LLDQLTRLKVEDRAQIGEISRQIRQIITEAEIPSDVVKAVAQYLSLFGEKYAYAVRSSAT 120 LLDQLT LKVEDR QIG ISR+IRQII E +IPSDVVKAVAQYLS FGE++AYAVRSSAT Sbjct: 61 LLDQLTMLKVEDRDQIGNISRKIRQIIMEVDIPSDVVKAVAQYLSQFGEEHAYAVRSSAT 120 Query: 121 AEDLPHASFAGQQDTYLNIIGKDAILQHISKCWASLFTERAVIYRMQNGFDHRQVYLSVV 180 AEDLPHASFAGQQDTYLNI G DAILQHISKCWASLFT+RAVIYRMQNGFDH QVYLSV+ Sbjct: 121 AEDLPHASFAGQQDTYLNITGVDAILQHISKCWASLFTDRAVIYRMQNGFDHSQVYLSVI 180 Query: 181 IQKMVFPQASGIVFTADPMTSNRKLLSIDASFGLGEALVSGLVSADCYKVREEEIVEKRI 240 +Q+MVFPQASGI+FTADP+TSNRK+LSIDA FGLGEALVSGLVSADC+KV++ +I++KRI Sbjct: 181 VQRMVFPQASGILFTADPITSNRKVLSIDAGFGLGEALVSGLVSADCFKVQDGQIIDKRI 240 Query: 241 ATKKLAIYGRQEGGTETRQLDPGQQTAQTLTDGEILELARIGRQIEAYFGQPQDIEWCLA 300 ATKK+AIYGR+EGGTET+Q+D QQ AQTLTD +IL+LARIGRQIEA+FGQPQDIEWCLA Sbjct: 241 ATKKMAIYGRKEGGTETQQIDSDQQKAQTLTDEQILQLARIGRQIEAHFGQPQDIEWCLA 300 Query: 301 DGTFYIVQSRPITTLYPIPEADDQENHVYVSVGHQQMMTDPIKPLGLSFYLLMTPALMRK 360 TFYIVQSRPITTL+PIPEA DQENHVY+SVGHQQMMTDPIKPLGLSF+LL T A MRK Sbjct: 301 RDTFYIVQSRPITTLFPIPEASDQENHVYISVGHQQMMTDPIKPLGLSFFLLTTVAPMRK 360 Query: 361 AGGRLFLDVA 370 AGGRLF+DV Sbjct: 361 AGGRLFVDVT 370 >CAC0534 [G] COG0574 Phosphoenolpyruvate synthase/pyruvate phosphate dikinase Length = 868 Score = 522 bits (1344), Expect = e-148, Method: Compositional matrix adjust. Identities = 240/370 (64%), Positives = 307/370 (82%) Query: 1 MSSLVLGFQEMDKTQLWLVGGKGLHLGELSKIQGIQVPEGFCVTTAGYQKAIEQNETYHA 60 M+S VLGF+E+DKT++ +VGGKG +LGELS++ GI +PEGFCVTT Y+K IE+++ ++ Sbjct: 1 MNSYVLGFKEIDKTKISMVGGKGANLGELSRVAGILIPEGFCVTTEAYKKIIEKSKEFNE 60 Query: 61 LLDQLTRLKVEDRAQIGEISRQIRQIITEAEIPSDVVKAVAQYLSLFGEKYAYAVRSSAT 120 LLD+L+ L++ED ++ E+S++IR +I IP D+V+ + YL+ GEK AYAVRSSAT Sbjct: 61 LLDELSCLRLEDGEKVYEVSKKIRMVIERISIPKDIVEEIDVYLTRLGEKDAYAVRSSAT 120 Query: 121 AEDLPHASFAGQQDTYLNIIGKDAILQHISKCWASLFTERAVIYRMQNGFDHRQVYLSVV 180 AEDLP ASFAGQQDTYLNIIGK +IL+HISKCWASLFT+RAVIYR+QNGFDHR+VY+SVV Sbjct: 121 AEDLPTASFAGQQDTYLNIIGKKSILEHISKCWASLFTDRAVIYRLQNGFDHRKVYISVV 180 Query: 181 IQKMVFPQASGIVFTADPMTSNRKLLSIDASFGLGEALVSGLVSADCYKVREEEIVEKRI 240 IQKM+FP+A+GI+FTADP+ SNRK+LSIDASFGLGEALVSGLV+AD YKV +I +K++ Sbjct: 181 IQKMIFPKAAGILFTADPVNSNRKVLSIDASFGLGEALVSGLVNADMYKVLSGKITDKKV 240 Query: 241 ATKKLAIYGRQEGGTETRQLDPGQQTAQTLTDGEILELARIGRQIEAYFGQPQDIEWCLA 300 + KKLAIY ++GGT+ ++L+ +Q Q LT+ +IL+L IGR IE +F PQDIEWCL Sbjct: 241 SKKKLAIYASKDGGTKAQELEIEKQNKQVLTEEQILQLEHIGRSIEEHFSFPQDIEWCLN 300 Query: 301 DGTFYIVQSRPITTLYPIPEADDQENHVYVSVGHQQMMTDPIKPLGLSFYLLMTPALMRK 360 TFYI+QSRPITTLYPIP+A+D+ENHVY+SVGHQQMMTDP+KPLG+S Y L + K Sbjct: 301 QDTFYILQSRPITTLYPIPKANDEENHVYISVGHQQMMTDPLKPLGMSLYELTSFGNRFK 360 Query: 361 AGGRLFLDVA 370 AGGRLF DV Sbjct: 361 AGGRLFFDVT 370 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,403,996 Number of extensions: 1095232 Number of successful extensions: 4453 Number of sequences better than 1.0e-05: 28 Number of HSP's gapped: 4394 Number of HSP's successfully gapped: 28 Length of query: 386 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 278 Effective length of database: 25,922,359 Effective search space: 7206415802 Effective search space used: 7206415802 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)