BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0560.0029.p (364 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value rfaB [M] COG0438 Glycosyltransferase 215 1e-55 STM3719 [M] COG0438 Glycosyltransferase 196 3e-50 aq_1641 [M] COG0438 Glycosyltransferase 100 5e-21 TM0622 [M] COG0438 Glycosyltransferase 86 1e-16 STM0622 [G] COG2301 Citrate lyase beta subunit 86 1e-16 >rfaB [M] COG0438 Glycosyltransferase Length = 369 Score = 215 bits (547), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 129/367 (35%), Positives = 203/367 (55%), Gaps = 24/367 (6%) Query: 7 SMRISIVCRELSGRGGMETVFKNVITHLMQKGDEVELIIF--GGSHDKKWLESVPHHI-I 63 SM+I+ + +SG GGMETV ++VIT Q+ + E+ F DK WLE + + Sbjct: 10 SMKIAFIGEAVSGFGGMETVIRDVITTFRQQHIQSEMFFFCRNDKMDKGWLEGIKYSCSF 69 Query: 64 EMPRSNLIKIYYNSLVVYSRLLRNFNPEVILVTDPYFIYQTKLVTSRMKRKPAIG----S 119 R ++ + S+ L+ + P++++ D + L ++ ++K I S Sbjct: 70 SNIRLGFLR-RAKHIHALSKWLQEYQPDIVICIDVI----SCLFAAKARKKSGIDMPVFS 124 Query: 120 WVHFELSALIKVQQLASAHFHLAISKGIGRQIEKVNGGRSDN-VHIIYNPVAISEMTVPR 178 W HF L + + A +HLAIS GI +Q+ +N G +++ +++I+NPV + +P Sbjct: 125 WPHFSLDHKKHAEYITCADYHLAISSGIKQQM--INRGVAESTINVIFNPVETKDSVIPA 182 Query: 179 PD---IATFLYVGRLEDE-YKKISDFLKALSQLKGEFKTIMIGDGPDHEPLVALSRQLGI 234 P+ ATF+YVGR++ E K++ D L LSQ KG +K ++GDG D E A R+L I Sbjct: 183 PEEGETATFIYVGRMKFEGQKRVKDLLDGLSQAKGNWKLHVLGDGSDFEKCQAYGRELNI 242 Query: 235 NDRVEWRGWQYNPWD----QLPPVSCLVLTSRLEGFGLVLVEAMSKGIPCISTDCPSGPS 290 +DR+ W GWQ PW+ + VS L+LTS EGF + L+EA+S GIPCIS DC SGP+ Sbjct: 243 DDRIVWYGWQQYPWELVQQDIEKVSALLLTSSFEGFPMTLLEALSWGIPCISADCVSGPA 302 Query: 291 EIIRGGENGWLVPPGDVGAISSMLQNVIDQPEILPSQEAVRESVKHFDTSLLMNKLRDVL 350 +II+ NG L PGD+ ++L I EI E + S+ F S ++L V+ Sbjct: 303 DIIQPDVNGHLYQPGDIAGFVTLLNKYI-AGEIHIEHEKIPASIDEFYQSKYYDRLHKVI 361 Query: 351 QAEINKK 357 + I+++ Sbjct: 362 ISAISRR 368 >STM3719 [M] COG0438 Glycosyltransferase Length = 359 Score = 196 bits (499), Expect = 3e-50, Method: Compositional matrix adjust. Identities = 123/365 (33%), Positives = 187/365 (51%), Gaps = 22/365 (6%) Query: 8 MRISIVCRELSGRGGMETVFKNVITHLMQKGDEVELIIF----GGSHDKKWLESVPH--- 60 M+I+ + +SG GGMETV NVI ++ +F DK WL+ + + Sbjct: 1 MKIAFIGEAVSGFGGMETVISNVIHTFENSSPKINCEMFFFCRNDKMDKAWLKEIKYAQS 60 Query: 61 -HIIEMPRSNLIKIYYNSLVVYSRLLRNFNPEVILVTDPYFIYQTKLVTSRMKRKPAIGS 119 I++ K YN +S+ L+ +P++++ D + + I S Sbjct: 61 FSNIKLSFLRRAKHVYN----FSQWLKETSPDIVICIDVISCLYANKARKKSGKHFTIFS 116 Query: 120 WVHFELSALIKVQQLASAHFHLAISKGIGRQIEKVNGGRSDNVHIIYNPVAISEMTVPRP 179 W HF L + + A +HLAIS GI QI G + ++ ++YNPV+I + VP P Sbjct: 117 WPHFSLDHKKHAECITYADYHLAISSGIKEQI-MARGISAQDISVVYNPVSIKTVIVPPP 175 Query: 180 DI---ATFLYVGRLEDE-YKKISDFLKALSQLKGEFKTIMIGDGPDHEPLVALSRQLGIN 235 + A FLYVGRL+ E K++ D L++ GE++ +IGDG D E A SR+LGI Sbjct: 176 ERDKPAVFLYVGRLKFEGQKRVKDLFDGLARTTGEWQLHIIGDGSDFEKCQAYSRELGIE 235 Query: 236 DRVEWRGWQYNPWD----QLPPVSCLVLTSRLEGFGLVLVEAMSKGIPCISTDCPSGPSE 291 RV W GWQ PW ++ V+ L+LTS EGF + L+EAMS GIPCIS+DC SGP + Sbjct: 236 QRVIWYGWQSAPWQVVQQKIKNVTALLLTSAFEGFPMTLLEAMSYGIPCISSDCMSGPRD 295 Query: 292 IIRGGENGWLVPPGDVGAISSMLQNVIDQPEILPSQEAVRESVKHFDTSLLMNKLRDVLQ 351 +I+ G NG L PG + L VI E+ + + +++ F L + + Sbjct: 296 MIKPGLNGELYTPGAIDDFVGHLNRVISG-EVKYQHDIIPGTIERFYDVLYFKNFNNAIF 354 Query: 352 AEINK 356 +++ K Sbjct: 355 SKLQK 359 >aq_1641 [M] COG0438 Glycosyltransferase Length = 442 Score = 100 bits (248), Expect = 5e-21, Method: Compositional matrix adjust. Identities = 7/209 (3%), Positives = 33/209 (15%), Gaps = 11/209 (5%) Query: 118 GSWVHFELSALIKVQQLASAHFHLAISKGIGRQIEKVNGGRSDNVHIIYNPVAISEMTVP 177 V ++ A + + + E+ Sbjct: 75 VVIVITGHGSVDTAV-KAIKKGAYEFLEKPFSVERFLLTIKHAFEEYSKKAPPQEEIEFV 133 Query: 178 RP-----DIATFLYVGRLEDEYKKISDFLKALSQLKGEFKTI---MIGDGPDHEPLVALS 229 +I + + + ++ Sbjct: 134 GEHPKILEIKRLIPKIAKSKA-PVLITGESGTGKEIVARLIHRYSGRKGAFVDLNCASIP 192 Query: 230 RQLGINDRVEWRGWQYNPWDQLPPVSCLVLTSRLEGFGLVLVEAMSKGIPCISTDCPSGP 289 ++L ++ + + V + Sbjct: 193 QELAESELFGHEKGAFTGALTRKKGKLELADQGTLFLDEVGELDQRVQAKLLRVLETG-S 251 Query: 290 SEIIRGGENGWLVPPGDVGAISSMLQNVI 318 + G + + ++ + + Sbjct: 252 FTRLGGNQKIEVDIRVISATNKNLEEEIK 280 >TM0622 [M] COG0438 Glycosyltransferase Length = 388 Score = 85.5 bits (210), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 56/182 (30%), Positives = 98/182 (53%), Gaps = 11/182 (6%) Query: 141 LAISKGIGRQIEKVNGGRSDNVHIIYNPVAISEMTVPRP-----DIATFLYVGRLEDEYK 195 ++IS+ + ++K+ G R + +IYN + + + ++ +P D + V RL E K Sbjct: 152 VSISQEVAESVKKLYG-RKISTPVIYNGIDVQKFSIDQPKRVDRDKTILINVARLSRE-K 209 Query: 196 KISDFLKALSQLKGEFKTI---MIGDGPDHEPLVALSRQLGINDRVEWRGWQYNPWDQLP 252 + ++A S+ + ++GDG + L +QLG+ ++V++ G + + + L Sbjct: 210 NHALLVRAFSKAVQSCPNLELWLVGDGELRRDIEELVKQLGLEEKVKFFGVRSDVPELLS 269 Query: 253 PVSCLVLTSRLEGFGLVLVEAMSKGIPCISTDCPSGPSEIIRGGENGWLVPPGDVGAISS 312 VL+S EGFGLV+ EAM+ G+P I+T G EI+ GG G LVPP DV A++ Sbjct: 270 QADIFVLSSDYEGFGLVVAEAMAAGLPVIAT-AIGGIPEILEGGRAGILVPPKDVDALAK 328 Query: 313 ML 314 + Sbjct: 329 AI 330 >STM0622 [G] COG2301 Citrate lyase beta subunit Length = 302 Score = 85.5 bits (210), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 0/182 (0%), Positives = 0/182 (0%), Gaps = 11/182 (6%) Query: 141 LAISKGIGRQIEKVNGGRSDNVHIIYNPVAISEMTVPRP-----DIATFLYVGRLEDEYK 195 A + IG + + + + D Sbjct: 152 HASERLIGIALGAEDY-VRNLRTERSPEGTELLFARCAILQAARSAGIQAFDTVYSDA-N 209 Query: 196 KISDFLKALSQLKGEFKTI---MIGDGPDHEPLVALSRQLGINDRVEWRGWQYNPWDQLP 252 + FL+ + +K + + + + + + Sbjct: 210 NEAGFLQEAAHIKQLGFDGKSLINPRQIELLHNLYAPTRKEVAHARLVVEAAEAAAREGL 269 Query: 253 PVSCLVLTSRLEGFGLVLVEAMSKGIPCISTDCPSGPSEIIRGGENGWLVPPGDVGAISS 312 V L +S+ Sbjct: 270 GVVSLNGKMVDSPVIERARLVLSRAELSGIR- 328 Query: 313 ML 314 Sbjct: 329 330 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.138 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,360,463 Number of extensions: 1154051 Number of successful extensions: 2831 Number of sequences better than 1.0e-05: 87 Number of HSP's gapped: 2793 Number of HSP's successfully gapped: 87 Length of query: 364 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 256 Effective length of database: 25,922,359 Effective search space: 6636123904 Effective search space used: 6636123904 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)