BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0560.0024.p (865 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_pksJ [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid... 259 1e-68 PA2424_1 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-aci... 226 9e-59 CC1165 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ... 220 8e-57 AGl2326 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid... 204 5e-52 alr2678_1 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-ac... 197 7e-50 >BS_pksJ [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Length = 346 Score = 259 bits (663), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 0/542 (0%), Positives = 0/542 (0%), Gaps = 42/542 (7%) Query: 11 KGITFINGDNEHNYYSYKEMYTRATGALRFLRSKGIQSGDEVIFQIENNETFILAFWACI 70 + T L S+ + N + + Sbjct: 46 LIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQAL 105 Query: 71 LGGMIPVPVNV----GNNDEHKHKLIKIWNVLHRPFLITDSRSLLKPDLT-AHQTDFYRF 125 I ++ D + F S + Sbjct: 106 DRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLG-YSWTDSAPAVSLLDTLDAL 164 Query: 126 ASSITNRTIHTETLEYSGTEDVVLCSPESHDIAFIQFSSGTTGDPKGVTLTHDNLLTNLY 185 A I G + + + Sbjct: 165 AEYQRAGKIRYI-----GVSNETAFGVM--RYLHLADKHDLPRIVTIQNPYSLLNRSFEV 217 Query: 186 DLKKSRNYSANDSFLSWMPLTHDMGMIVFHLLPLTCGGSQSLLPTWVFIRRPTLWIKKAS 245 L + Y + + + + + T A Sbjct: 218 GLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAY 277 Query: 246 DLKSTVIASPNFGMKYFLTFFYRSQCSYEWDLSNVRVLLDAAEPIDAKLCNEFLEVMDKF 305 + M + + + L +E + + Sbjct: 278 VDIARRHGLDPAQMA-LAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEIEA 336 Query: 306 SLPRTSLLPGYGLAEATAAVTLYKPGHEFTSYNIVRSSINTGSRVQIVEENYKDKEYIVS 365 Sbjct: 337 ------------------------ 372 Query: 366 LVDLGNPIENCFVRVCDEEDRVLDECVVGHVQIKGNNVTNGYYNNEEATRKVFTKDGWLR 425 Sbjct: 373 432 Query: 426 TGDLGFMKDGSLIVTGREKDIIFINGQNFYPQDIERVAEEFTGTRIGVTAACGVYNEQLA 485 Sbjct: 433 492 Query: 486 TEEVILF--VNYKDKPETFVPLAIELRKQLNLKVGIHVRHIIPVKK--ILKTTSGKVQRY 541 Sbjct: 493 552 Query: 542 KM 543 Sbjct: 553 554 >PA2424_1 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Length = 346 Score = 226 bits (577), Expect = 9e-59, Method: Compositional matrix adjust. Identities = 0/669 (0%), Positives = 0/669 (0%), Gaps = 66/669 (9%) Query: 19 DNEHNYYSYKEMYTRATGALRFLRSKGIQSGDEVIFQIENNETFILAFWACILGGMIPVP 78 ++ + A Q E + P Sbjct: 35 QLDYAVAQGINLIDVAE-MYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSR 93 Query: 79 VNVGNNDE--HKHKLIKIWNVLHRPFLITDSRSLLKPDLTAHQTDFYRFASSITNRTIHT 136 N + + + L + Q R + Sbjct: 94 NNDKGIRPDQALDRKNIREALHD-SLKRLQTDYLDLYQVHWPQ----RPTNCFGKLGYSW 148 Query: 137 ETLEYSGTE--DVVLCSPESHDIAFIQFSSGTTGDPKGVTLTHDNLLTNLYDLKKSRNYS 194 + + + + A G + + + + +L + + Sbjct: 149 TDSAPAVSLLDTLDALAEYQ--RAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQN 206 Query: 195 ANDSFLSWMPLTHDMGMIVFHLLPLTCGGSQSLLPTWVFIRRPTLWIKKASDLKSTVIAS 254 + + L T ++ + + S Sbjct: 207 PYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYS 266 Query: 255 PNFGMKYFLTFFYRSQCSYEWDLSNVRVLLDAAEPIDAKLCNEFLEVMDKFSLPRTSLLP 314 K + ++ D + + + +P A + + + L Sbjct: 267 GEQTQKAVAAYVDIARRH-GLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLE 325 Query: 315 GYGLAEATAAVTLYKPGHEFTSYNI-----VRSSINTGSRVQIVEENYKDKEYIVSLVDL 369 Sbjct: 326 385 Query: 370 GNPIENCFVRVCDEEDRVLDECVVGHVQIKGNNVTNGYYNNEEATRKVFT-KDG--WLRT 426 Sbjct: 386 ------------- 432 Query: 427 GDLGFMKDGSLIVTGREKDIIFINGQNFYPQDIERVAE-EFTGTRIGVTAACGVYNEQLA 485 Sbjct: 433 -- 490 Query: 486 TEEVILFVNYKDKPETFVPLAIELRKQLNLKVGIHVRHIIPVKKIL------KTTSGKVQ 539 Sbjct: 491 - 549 Query: 540 RYKMKNLFLEGQ------FTGLLAEMEEVTNTITSRIDADQPK---DTVEAALLSIWANV 590 Sbjct: 550 ------------ 597 Query: 591 LGNDRLGVKDNFFEIGGTSTLLAQMFEQVDREYPGKITLTDLFGHPSISKLAGLI-RQDG 649 Sbjct: 598 657 Query: 650 SDINASAHI 658 Sbjct: 658 666 >CC1165 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Length = 346 Score = 220 bits (560), Expect = 8e-57, Method: Compositional matrix adjust. Identities = 0/537 (0%), Positives = 0/537 (0%), Gaps = 30/537 (5%) Query: 27 YKEMYTRATGALRFLRSKGIQSGDEVIFQIENNETFILAFWACILGGMIPVPVNVGNNDE 86 G+ + + I + + D Sbjct: 48 DVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDR 107 Query: 87 HKHKLIKIWNVLHRPFLITDSRSLLKPDLTAHQTDFYRFASSITNRTIHTETLEYSGT-- 144 + ++ R + Sbjct: 108 KNIREALHDSLKRLQ---------TDYLDLYQVHWPQRPTNCFGKLG----YSWTDSAPA 154 Query: 145 ---EDVVLCSPE--SHDIAFIQFSSGTTGDPKGVTLTHDNLLTNLYDLKK-SRNYSANDS 198 D + E S T L + + + N S Sbjct: 155 VSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRS 214 Query: 199 FLSWMPLTHDMGMIVFHLLPLTCGGSQSLLPTWVFIRRPTLWIKKASDLKSTVIASPNFG 258 F + + G+ + + + + + Sbjct: 215 FEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAV 274 Query: 259 MKYFLTFFYRSQCSYEWDLSNVRVLLDAAEPIDAKLCNEFLEVMDKFSLPRTSLLPGYGL 318 ++ R + SL Sbjct: 275 AA-YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAE 333 Query: 319 AEATAAVTLYKPGHEFTSYNIVRSSINTGSRVQIVEENYKDKEYIVSLVDLGNPIENCFV 378 Sbjct: 334 --- 390 Query: 379 RVCDEEDRVLDECVVGHVQIKGNNVTNGYYNNEEATRKVFTKDGWLRTGDLGFMKDGSLI 438 Sbjct: 391 450 Query: 439 VTGREKDIIFINGQNFYPQDIERVAE-EFTGTRIGVTAACGVYNEQLATEEVILFVNYK- 496 Sbjct: 451 -- 508 Query: 497 DKPETFVPLAIELRKQLNLKVGIHVR-HIIPVKKILKTTSGKVQRYKMKNLFLEGQF 552 Sbjct: 509 565 >AGl2326 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Length = 346 Score = 204 bits (519), Expect = 5e-52, Method: Compositional matrix adjust. Identities = 0/659 (0%), Positives = 0/659 (0%), Gaps = 45/659 (6%) Query: 18 GDNEHNYYSYKEMYTRATGALRFLRSKG-IQSGDEVIFQIENNETFILAFWACILGGMIP 76 + + +N + + + Sbjct: 68 YVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLD 127 Query: 77 VPVNVGNNDEHKHKLIKIWNVLHRPFLITDSRSLLKPDLTAHQTDF-YRFASSITNRTIH 135 + ++ ++ +L Y S+ T + Sbjct: 128 LYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVM 187 Query: 136 TETLEYSGTEDVVLCSPESHDIAFIQFSSGTTGDPKGVTLTHDNLLTNLYDLKKSRNYSA 195 + + + + + V+ L L Sbjct: 188 RYLHLADKHDLPRI---VTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGK 244 Query: 196 NDSFLSWMPLTHDMGMIVFHLLPLTCGGSQSLLPTWVFIRRPTLWIKKASDLKSTVIASP 255 + + + + + + ++ + Sbjct: 245 YLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVAS 304 Query: 256 NFGMKYFLTFFYRSQCSYEWDLSNVRVLLDAAEPIDAKLCNEFLEVMDKFSLPRTSLLPG 315 Sbjct: 305 TLLGAT- 363 Query: 316 YGLAEATAAVTLYKPGHEFTSYNIVRSSI-NTGSRVQIVEENYKDKEYIVSLVDLGNPIE 374 Sbjct: 364 -- 421 Query: 375 NCFVRVCD-EEDRVLDECVVGHVQIKGNNVTNGYYNNEEAT-----RKVFTKDGWLRTGD 428 Sbjct: 422 481 Query: 429 LGFMKDGSLIVTGREKDIIFINGQNFYPQDIERVAEEFTGTRIGVTAACGVYNEQLATEE 488 Sbjct: 482 -- 539 Query: 489 VILFV--------NYKDKPETFVPLAIELRKQLNLKVGIHVRHIIPVKKILKTTSGKVQR 540 Sbjct: 540 -- 597 Query: 541 YKMKNLFLEGQFTGL----LAEMEEVTNTITSRIDADQPKDTVE------AALLSIW--- 587 Sbjct: 598 657 Query: 588 -----ANVLGNDRLGVKDNFFEIGGTSTLLAQMFEQVDREYPGKITLTDLFGHPSISKL 641 Sbjct: 658 716 >alr2678_1 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Length = 346 Score = 197 bits (501), Expect = 7e-50, Method: Compositional matrix adjust. Identities = 0/564 (0%), Positives = 0/564 (0%), Gaps = 85/564 (15%) Query: 19 DNEHNYYSYKEMYTRATGALRFLRSKGIQSGDEVIFQIENNETFILAFWACILGGMIPVP 78 N EMY ++ G+ + + I + + Sbjct: 41 AQGINLIDVAEMYPVPPRPETQGLTETYV-GNWLAKHGSREKLIIASKVSGPSRNNDKGI 99 Query: 79 VNVGNNDEHKHKLIKIWNVLHRPFLITDSRSLLKPDLTAHQTDFYRFASSITNRTIHTET 138 D + ++ D + P + Sbjct: 100 RPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGK--------------LG 145 Query: 139 LEYSGTEDVVLCSPES--------HDIAFIQFSSGTTGDPKGVTLTHDNLLTNLYDLKKS 190 ++ + V S T L + + Sbjct: 146 YSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQ 205 Query: 191 RNYSANDSFLSWMPLTHDMGMIVFHLLPLTCGGSQSLLPTWVFIRRPTLWIKKASDLKST 250 YS + V L G + S Sbjct: 206 NPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRY 265 Query: 251 VIASPNFGMKYFLTFFYRSQCSYEWDLSNVRVLLDAAEPIDAKLCNEFLEVMDKFSLPRT 310 + ++ R ++ V ++ Sbjct: 266 SGEQTQKAVAAYVDIARRHGL-DPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHL 324 Query: 311 SLLPGYGLAEATAAVTLYKPGHEFTSYNIVRSSINTGSRVQIVEENYKDKEYIV------ 364 L Sbjct: 325 ELSEDVLAEIEAVHQV------------------------------ 354 Query: 365 SLVDLGNPIENCFVRVCDEEDRVLDECVV---GHVQIKGNNVTNGYYNNEEATRKVFT-- 419 Sbjct: 355 -- 412 Query: 420 ----KDGWLRTGDLGFMKDGSLIVTGREKDIIFINGQNFYPQDIERVAEEFTGTRIGVTA 475 Sbjct: 413 --- 469 Query: 476 ACG-VYNEQLATEEVILFV------NYKDKPETFVPLAIELRKQLNLKVGIHVRHIIPVK 528 Sbjct: 470 -- 527 Query: 529 --KILKTTSGKVQRYKMKNLFLEG 550 Sbjct: 528 551 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.137 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 64,762,172 Number of extensions: 2852587 Number of successful extensions: 7992 Number of sequences better than 1.0e-05: 167 Number of HSP's gapped: 7769 Number of HSP's successfully gapped: 190 Length of query: 865 Length of database: 38,378,431 Length adjustment: 115 Effective length of query: 750 Effective length of database: 25,115,021 Effective search space: 18836265750 Effective search space used: 18836265750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 120 (50.8 bits)