BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0560.0016.p (328 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC0372 [T] COG0642 Signal transduction histidine kinase 221 2e-57 BH0754 [T] COG0642 Signal transduction histidine kinase 168 1e-41 CAC0225 [T] COG0642 Signal transduction histidine kinase 166 4e-41 lin1852 [T] COG0642 Signal transduction histidine kinase 132 9e-31 ML0132 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ... 125 8e-29 >CAC0372 [T] COG0642 Signal transduction histidine kinase Length = 334 Score = 221 bits (562), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 106/317 (33%), Positives = 197/317 (62%), Gaps = 11/317 (3%) Query: 21 YVLNTVILVMASYVFYGVNDILYPLFVSFFILLVYLAVTAVKLNQFTGKLSAAKSSPHIE 80 + L++ I++ YV + D++YP+F+ I ++++ K +F L+ + + Sbjct: 20 FFLSSGIILFYFYVSKNITDVVYPIFMVVSIYIIFIVTEWFKYYRFNANLTRGIEREYYD 79 Query: 81 VDDTNVKDKLIFGAINEIHEQYNGRLHQMNSSIKERNTLFSQWIHNMKVSISIIGLAAEK 140 ++ + ++ AI +IH+ Y ++ +++ + SQWIHNMK +S+I L ++ Sbjct: 80 LESVTPEQRMAQEAILKIHKNYAAKISEIDYKNSDIKYFISQWIHNMKTPVSVIDLIVQR 139 Query: 141 GTE----EAISDIKEENRRLTQNLEECLNLLRLDDFARDYLPEKVNLHKVVNKAVNARKR 196 E + I +I+EEN ++ +++ LN++RLD+F+RDY PE V++ ++V + +N +K Sbjct: 140 EKEKLSGDVIKNIEEENYKIKNGMDQVLNIIRLDEFSRDYEPEIVDIVELVKRTINLKKN 199 Query: 197 EFIYKGVYPQIDIAKHLE---ICTDEKWCGYMLEQVLSNAVKYSDKE--GRVTVRSELAG 251 +FIY +P+++ H++ + TD+KW ++++Q++SN++KYS KE G V + Sbjct: 200 QFIYGNAFPKVEF--HVDKALVLTDKKWSTFIIDQIISNSIKYSKKEEKGYVYFNIVQSN 257 Query: 252 DRVLLSIQDYGVGIDQEDLPRVFDPFFTGKNGRDYRSATGIGLYMVKHIARKLGHRVSID 311 ++ L I+D GVGI + DL R+F+PFFTG+NGR++ ++TGIGLY+ K IA LGH +S++ Sbjct: 258 NKTHLVIKDNGVGIPKYDLKRIFEPFFTGENGRNFENSTGIGLYICKKIADNLGHEISVE 317 Query: 312 SQKGAGTRVTFAFLSKV 328 S+ GT V +++KV Sbjct: 318 SEINKGTIVKITYMAKV 334 >BH0754 [T] COG0642 Signal transduction histidine kinase Length = 353 Score = 168 bits (425), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 110/336 (32%), Positives = 177/336 (52%), Gaps = 23/336 (6%) Query: 5 RRIAELLHVQKGMLLVYVLNTVILVMASYVFYGVNDILYPLFVSFFILLVYLAVTAVKLN 64 RR L ++ +L++ V+ + + +F I Y +S L ++L ++ Sbjct: 10 RRFYSLFYIGNTLLILLVIK--LALNEEGLFISNATIGYIGLLSVVSLALFLFADYIRQR 67 Query: 65 QFTGKLSAAKSSPHIE----VDDTNVKDKL----IFGAINEIHEQYNGRLHQMNSSIKER 116 F +L AK + +E V + ++L +FG + E Y+ RLH Sbjct: 68 PFFQELRQAKENVSLESLLKVKEGITNEQLAMIKLFG---QAFEDYSTRLHTAQEQHSRS 124 Query: 117 NTLFSQWIHNMKVSISIIGLAAEKGTEEA----ISDIKEENRRLTQNLEECLNLLRLDDF 172 ++W H MK +S+I L ++ E + + I+EE +L L L+ +RL F Sbjct: 125 YHFANRWTHQMKTPLSVIRLILQEQDEWSKEVLVDQIEEEVGKLEDGLHMMLSTVRLQKF 184 Query: 173 ARDYLPEKVNLHKVVNKAVNARKREFIYKGVYPQ-IDIAKHLEICTDEKWCGYMLEQVLS 231 D+ E++ L V+ ++ +++ FI +YP+ I A+ + TD+KW ++LEQ++ Sbjct: 185 ELDFSIERIALLSVIRSVLHGKRKTFIRYRIYPRLISEAEEVFAATDKKWLIFVLEQLIQ 244 Query: 232 NAVKYSDKEGR-----VTVRSELAGDRVLLSIQDYGVGIDQEDLPRVFDPFFTGKNGRDY 286 NA+KYS R +TVR E + V +SIQD G+GI EDLPRVFDPFFTG+NGR Sbjct: 245 NALKYSSTLAREPSKYITVRVEETDNGVTVSIQDEGIGIVAEDLPRVFDPFFTGENGRKL 304 Query: 287 RSATGIGLYMVKHIARKLGHRVSIDSQKGAGTRVTF 322 + +TG+GLY+VK I +LGH ++I+S GT VT Sbjct: 305 QESTGMGLYLVKQICDQLGHSITIESTVNEGTTVTL 340 >CAC0225 [T] COG0642 Signal transduction histidine kinase Length = 339 Score = 166 bits (421), Expect = 4e-41, Method: Compositional matrix adjust. Identities = 93/290 (32%), Positives = 165/290 (56%), Gaps = 5/290 (1%) Query: 39 NDILYPLFVSFFILLVYLAVTAVKLNQFTGKLSA---AKSSPHIEVDDTNVKDKLIFGAI 95 ++I Y +FV FFILL++L V N+ KL A K I + KD++ I Sbjct: 39 SNIGYMIFVMFFILLIFLCVDYSIKNKHIKKLIANGEIKDKTPILPEPLEYKDEVYISII 98 Query: 96 NEIHEQYNGRLHQMNSSIKERNTLFSQWIHNMKVSISIIGLAAEKGTEEAISDIKEENRR 155 ++++ YN R+ + ++ N + WIH +K I+ L E G ++ EE + Sbjct: 99 EDLYKDYNERIVSLEKEFEDNNEFMTAWIHEIKTPIATAKLLLESGEIDS-QLFMEEIEK 157 Query: 156 LTQNLEECLNLLRLDDFARDYLPEKVNLHKVVNKAVNARKREFIYKGVYPQIDIAKHLEI 215 + +E+ L R D+F++DY+ +VN+ K++ + + + + FI K + +++I + + Sbjct: 158 IDDYVEKVLYYSRSDNFSKDYIISEVNISKLIKECIKSHSKIFIKKHIKLKLEIDERFNV 217 Query: 216 CTDEKWCGYMLEQVLSNAVKYSDKEGRVTVRSELAGDRVLLSIQDYGVGIDQEDLPRVFD 275 TD+KW ++L Q++SNA+KY++ G + +++ + ++ I+D G+GI +EDL R+F Sbjct: 218 ETDKKWFLFILNQIISNALKYTNDMGNIIIKAFEDDNEKVVIIEDNGIGIKKEDLDRIFS 277 Query: 276 PFFTGKNGRDYRS-ATGIGLYMVKHIARKLGHRVSIDSQKGAGTRVTFAF 324 FTG NGR S ATG+GLY+ +A KLGH ++I+S+ GT++ F Sbjct: 278 KSFTGYNGRKENSKATGMGLYLSNKLAEKLGHNITIESKYNEGTKLYIHF 327 >lin1852 [T] COG0642 Signal transduction histidine kinase Length = 182 Score = 132 bits (331), Expect = 9e-31, Method: Compositional matrix adjust. Identities = 0/236 (0%), Positives = 0/236 (0%), Gaps = 10/236 (4%) Query: 99 HEQYNGRLHQMNSSIKERNTLFSQWIHNMKVSI----SIIGLAAEKGTEEAISDIKEENR 154 + + S N + + + Sbjct: 105 RATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSPWMVM 164 Query: 155 RLTQNLEECLNLLRLDDFARDYLPEKVNLHKVVNKAVNARKREFIYKGVYPQIDIAK-HL 213 Sbjct: 165 -- 222 Query: 214 EICTDEKWCGYMLEQVLSNAVKYSDKEGRVTVR--SELAGDRVLLSIQDYGVGIDQEDLP 271 Sbjct: 223 - 281 Query: 272 RVFDPFFTGKNGRDYRSATGIGLYMVKHIARKLGHRVSIDSQKGAGTRVTFAFLSK 327 Sbjct: 282 337 >ML0132 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Length = 211 Score = 125 bits (314), Expect = 8e-29, Method: Compositional matrix adjust. Identities = 0/223 (0%), Positives = 0/223 (0%), Gaps = 9/223 (4%) Query: 99 HEQYNGRLHQMNSSIKERNTLFSQWIHNMKVSISIIGLAAEKGTEEAISDIKEENRRLTQ 158 L+ + + HN +V L A Sbjct: 74 TYIKTIGLYNSKAENIIKTCRILLEQHNGEVPEDRAALEAL--PGVGRKTANVVLNTAFG 131 Query: 159 NLEECLNLLRLDDFARDYLPEKVNLHKVVNKAVNARKREFIYKGVYPQIDIAKHLEICTD 218 ++ R N+ +V K + EF + I C Sbjct: 132 WPTIAVDTHIFRVCNRTQFAPGKNVEQVEEKLLKVVPAEFKVDCHHWLIL--HGRYTCIA 189 Query: 219 EKWCGYMLEQVLSNAVKYSDKEGRVTVRSELAGDRVLLSIQDYGVGIDQEDLPRVFDPFF 278 K K Sbjct: 190 RKPRCGSCIIEDLCEYKEK----- 244 Query: 279 TGKNGRDYRSATGIGLYMVKHIARKLGHRVSIDSQKGAGTRVT 321 Sbjct: 245 287 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.138 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,268,655 Number of extensions: 963969 Number of successful extensions: 2606 Number of sequences better than 1.0e-05: 147 Number of HSP's gapped: 2572 Number of HSP's successfully gapped: 147 Length of query: 328 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 221 Effective length of database: 26,037,693 Effective search space: 5754330153 Effective search space used: 5754330153 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 116 (49.3 bits)