BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0557.0099.p (298 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BMEI1818 [L] COG1643 HrpA-like helicases 208 9e-54 PA3961 [L] COG1643 HrpA-like helicases 207 1e-53 XF1229 [L] COG1643 HrpA-like helicases 197 1e-50 AGc867 [S] COG1284 Uncharacterized conserved protein 191 1e-48 AGc866 [R] COG4976 Predicted methyltransferase (contains TPR rep... 191 1e-48 >BMEI1818 [L] COG1643 HrpA-like helicases Length = 710 Score = 208 bits (529), Expect = 9e-54, Method: Compositional matrix adjust. Identities = 121/253 (47%), Positives = 153/253 (60%), Gaps = 5/253 (1%) Query: 1 MKQLPITQVLPELKTILNTSTAAVLTAEPGAGKTTGTPPAFLDEPWMAGKTILMLEPRRL 60 + LP+T++LP+L L +A+L A PGAGKTT P L W I++LEPRRL Sbjct: 96 LPDLPVTRILPQLDEALAHHASALLVAPPGAGKTTLVPLHMLGADWRGEGVIVLLEPRRL 155 Query: 61 XXXXXXXXXXXXXXXXXXQTVGYRMRNDTKVGPDTRIVVVTEGVLTRMLQSDPSLGEVGL 120 +TVGYRMR ++KV TRI+VVTEGV RM+ DP L V Sbjct: 156 AARAAARRMAQLLGEEPGETVGYRMRLESKVSAKTRILVVTEGVFARMILDDPDLNGVAA 215 Query: 121 VIFDEFHERSLHADLGLALTLEAQAVLREDLRILIMSATLDSGRVSAMLXXXXGAPVVNC 180 V+FDEFHERSL AD GLAL L+ QA LR+DL+IL+MSATLD RV+++L APV+ Sbjct: 216 VLFDEFHERSLDADFGLALALDVQAALRDDLKILVMSATLDGARVASLLGD---APVIES 272 Query: 181 PGRTFPVETIYLPGSGDRQLEQAXXXXAVRRALAEQPGDVLVFLPGEREIRRTGRELESG 240 GR +PV+ Y D Q+E A A+R AL ++ G +L FLPG+ EI RT R LE Sbjct: 273 EGRAYPVDIRYRDRKADEQIEDA-MARAIRDALVDETGSILAFLPGQGEIERTARLLEE- 330 Query: 241 TLPPGTVLRPLYG 253 LP +L PLYG Sbjct: 331 RLPANVLLAPLYG 343 >PA3961 [L] COG1643 HrpA-like helicases Length = 838 Score = 207 bits (527), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 129/257 (50%), Positives = 163/257 (63%), Gaps = 6/257 (2%) Query: 1 MKQLPITQVLPELKTILNTSTAAVLTAEPGAGKTTGTPPAFLDEPWMAGKTILMLEPRRL 60 M LPI V+P L+ L AVL A PGAGKTT P A LDEPW+AG+ ILMLEPRRL Sbjct: 1 MISLPIDAVVPALRQALGAQHQAVLEAPPGAGKTTRVPLALLDEPWLAGQRILMLEPRRL 60 Query: 61 XXXXXXXXXXXXXXXXXXQTVGYRMRNDTKVGPDTRIVVVTEGVLTRMLQSDPSLGEVGL 120 +TVGYR+R +++VGP TRI VVTEG+L R LQ DP+L VGL Sbjct: 61 AARAAAERLAAELGEKVGETVGYRIRLESRVGPKTRIEVVTEGILARRLQDDPALDGVGL 120 Query: 121 VIFDEFHERSLHADLGLALTLEAQAVLRED--LRILIMSATLDSGRVSAMLXXXXGAPVV 178 VIFDEFHERSL ADL LALTL + +LR++ L++L+MSATL+ R++A+L APVV Sbjct: 121 VIFDEFHERSLDADLALALTLNGRELLRDEPPLKVLVMSATLEGERLAALLGE---APVV 177 Query: 179 NCPGRTFPVETIYLPGSGDRQLEQAXXXXAVRRALAEQPGDVLVFLPGEREIRRTGREL- 237 GR FPV+ + + + + AV +ALAE+ G VLVFLPG+ EIRR L Sbjct: 178 RSEGRMFPVDIRWGRPAQPGEFIEPRVQQAVLQALAEESGSVLVFLPGQAEIRRVHEGLR 237 Query: 238 ESGTLPPGTVLRPLYGQ 254 E+ P +L PL+G+ Sbjct: 238 EALGGRPEVLLCPLHGE 254 >XF1229 [L] COG1643 HrpA-like helicases Length = 833 Score = 197 bits (502), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 114/251 (45%), Positives = 148/251 (58%), Gaps = 6/251 (2%) Query: 4 LPITQVLPELKTILNTSTAAVLTAEPGAGKTTGTPPAFLDEPWMAGKTILMLEPRRLXXX 63 LPI +LP + T L+ +L A PGAGKTT P A LD PW+ G++I++LEPRR+ Sbjct: 6 LPIDPLLPAITTSLSNHPRLILEAPPGAGKTTQVPLALLDAPWLNGRSIILLEPRRVAAR 65 Query: 64 XXXXXXXXXXXXXXXQTVGYRMRNDTKVGPDTRIVVVTEGVLTRMLQSDPSLGEVGLVIF 123 VGY +R ++KV TRI VVTEG+LTR++Q DP L +G ++F Sbjct: 66 NAALFMARQCGEEVGGLVGYHIRFESKVSARTRIQVVTEGILTRIIQDDPLLDGIGALLF 125 Query: 124 DEFHERSLHADLGLALTLEAQAVLREDLRILIMSATLDSGRVSAMLXXXXGAPVVNCPGR 183 DEFHER L +DLGLAL L+ Q LREDLRI++MSATL R++ L AP + GR Sbjct: 126 DEFHERHLASDLGLALALDVQTQLREDLRIVVMSATLQGERLAQFL----DAPRLTSEGR 181 Query: 184 TFPVETIYLPGSGDRQLEQAXXXXAVRRALAEQPGDVLVFLPGEREIRRTGRELESGTLP 243 +PV + P D LE A++ AL GDVL FLPG+REI R LE TL Sbjct: 182 AYPVAITHFPARRDESLE-THARRAIQHALDTHQGDVLTFLPGQREIARLQAILEK-TLS 239 Query: 244 PGTVLRPLYGQ 254 P + PL+G+ Sbjct: 240 PELHVMPLHGE 250 >AGc867 [S] COG1284 Uncharacterized conserved protein Length = 866 Score = 191 bits (486), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 117/254 (46%), Positives = 146/254 (57%), Gaps = 5/254 (1%) Query: 1 MKQLPITQVLPELKTILNTSTAAVLTAEPGAGKTTGTPPAFLDEPWMAGKTILMLEPRRL 60 + +LP++ VL ++ +L AVL+A PGAGKTT P L E W I++LEPRRL Sbjct: 50 LPELPVSAVLGDIAQVLAAKKRAVLSAPPGAGKTTLVPLRLLHEDWRGDGKIILLEPRRL 109 Query: 61 XXXXXXXXXXXXXXXXXXQTVGYRMRNDTKVGPDTRIVVVTEGVLTRMLQSDPSLGEVGL 120 +TVGYRMR D +V TRI VVTEGV RM+ DP L V Sbjct: 110 AARAAAGRMAELLRENVGETVGYRMRLDNRVSSKTRIEVVTEGVFARMVLDDPELSGVST 169 Query: 121 VIFDEFHERSLHADLGLALTLEAQAVLREDLRILIMSATLDSGRVSAMLXXXXGAPVVNC 180 VIFDEFHERSL D GLAL L+ QA LREDLRIL+MSATLD R+ A++ APV+ Sbjct: 170 VIFDEFHERSLDGDFGLALALDVQAGLREDLRILVMSATLDVERIGALM---GDAPVIQS 226 Query: 181 PGRTFPVETIYLPGSGDRQLEQAXXXXAVRRALAEQPGDVLVFLPGEREIRRTGRELESG 240 GRTFP++ Y +++ A+ A A + G +L FLPG+ EI RT LE G Sbjct: 227 LGRTFPIDIRYEERWQGIHVDE-KVAKAITEAHASETGSILAFLPGQAEITRTASRLE-G 284 Query: 241 TLPPGTVLRPLYGQ 254 P T + PLYG Sbjct: 285 RFPDDTDIIPLYGN 298 >AGc866 [R] COG4976 Predicted methyltransferase (contains TPR repeat) Length = 866 Score = 191 bits (486), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 117/254 (46%), Positives = 146/254 (57%), Gaps = 5/254 (1%) Query: 1 MKQLPITQVLPELKTILNTSTAAVLTAEPGAGKTTGTPPAFLDEPWMAGKTILMLEPRRL 60 + +LP++ VL ++ +L AVL+A PGAGKTT P L E W I++LEPRRL Sbjct: 50 LPELPVSAVLGDIAQVLAAKKRAVLSAPPGAGKTTLVPLRLLHEDWRGDGKIILLEPRRL 109 Query: 61 XXXXXXXXXXXXXXXXXXQTVGYRMRNDTKVGPDTRIVVVTEGVLTRMLQSDPSLGEVGL 120 +TVGYRMR D +V TRI VVTEGV RM+ DP L V Sbjct: 110 AARAAAGRMAELLRENVGETVGYRMRLDNRVSSKTRIEVVTEGVFARMVLDDPELSGVST 169 Query: 121 VIFDEFHERSLHADLGLALTLEAQAVLREDLRILIMSATLDSGRVSAMLXXXXGAPVVNC 180 VIFDEFHERSL D GLAL L+ QA LREDLRIL+MSATLD R+ A++ APV+ Sbjct: 170 VIFDEFHERSLDGDFGLALALDVQAGLREDLRILVMSATLDVERIGALM---GDAPVIQS 226 Query: 181 PGRTFPVETIYLPGSGDRQLEQAXXXXAVRRALAEQPGDVLVFLPGEREIRRTGRELESG 240 GRTFP++ Y +++ A+ A A + G +L FLPG+ EI RT LE G Sbjct: 227 LGRTFPIDIRYEERWQGIHVDE-KVAKAITEAHASETGSILAFLPGQAEITRTASRLE-G 284 Query: 241 TLPPGTVLRPLYGQ 254 P T + PLYG Sbjct: 285 RFPDDTDIIPLYGN 298 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.139 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,145,450 Number of extensions: 736526 Number of successful extensions: 1444 Number of sequences better than 1.0e-05: 44 Number of HSP's gapped: 1305 Number of HSP's successfully gapped: 44 Length of query: 298 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 192 Effective length of database: 26,153,027 Effective search space: 5021381184 Effective search space used: 5021381184 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)